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Protein

Synaptic vesicular amine transporter

Gene

SLC18A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.

GO - Molecular functioni

GO - Biological processi

  • aminergic neurotransmitter loading into synaptic vesicle Source: GO_Central
  • chemical synaptic transmission Source: ParkinsonsUK-UCL
  • dopamine transport Source: ParkinsonsUK-UCL
  • locomotory behavior Source: Ensembl
  • monoamine transport Source: ProtInc
  • negative regulation of reactive oxygen species biosynthetic process Source: ParkinsonsUK-UCL
  • neurotransmitter loading into synaptic vesicle Source: ParkinsonsUK-UCL
  • neurotransmitter secretion Source: Reactome
  • post-embryonic development Source: Ensembl
  • response to amphetamine Source: Ensembl
  • sequestering of neurotransmitter Source: ParkinsonsUK-UCL

Keywordsi

Biological processNeurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-181429 Serotonin Neurotransmitter Release Cycle
R-HSA-181430 Norepinephrine Neurotransmitter Release Cycle
R-HSA-212676 Dopamine Neurotransmitter Release Cycle
R-HSA-442660 Na+/Cl- dependent neurotransmitter transporters
SIGNORiQ05940

Protein family/group databases

TCDBi2.A.1.2.29 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:SLC18A2
Synonyms:SVMT, VMAT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000165646.11
HGNCiHGNC:10935 SLC18A2
MIMi193001 gene
neXtProtiNX_Q05940

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 129Lumenal, vesicleSequence analysisAdd BLAST88
Transmembranei130 – 150HelicalSequence analysisAdd BLAST21
Topological domaini151 – 159CytoplasmicSequence analysis9
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 189Lumenal, vesicleSequence analysis9
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 219CytoplasmicSequence analysis9
Transmembranei220 – 242HelicalSequence analysisAdd BLAST23
Topological domaini243 – 248Lumenal, vesicleSequence analysis6
Transmembranei249 – 271HelicalSequence analysisAdd BLAST23
Topological domaini272 – 291CytoplasmicSequence analysisAdd BLAST20
Transmembranei292 – 311HelicalSequence analysisAdd BLAST20
Topological domaini312 – 328Lumenal, vesicleSequence analysisAdd BLAST17
Transmembranei329 – 352HelicalSequence analysisAdd BLAST24
Topological domaini353 – 357CytoplasmicSequence analysis5
Transmembranei358 – 378HelicalSequence analysisAdd BLAST21
Topological domaini379 – 389Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei390 – 410HelicalSequence analysisAdd BLAST21
Topological domaini411 – 414CytoplasmicSequence analysis4
Transmembranei415 – 435HelicalSequence analysisAdd BLAST21
Topological domaini436 – 440Lumenal, vesicleSequence analysis5
Transmembranei441 – 462HelicalSequence analysisAdd BLAST22
Topological domaini463 – 514CytoplasmicSequence analysisAdd BLAST52

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6571
MalaCardsiSLC18A2
OpenTargetsiENSG00000165646
Orphaneti352649 Brain dopamine-serotonin vesicular transport disease
PharmGKBiPA325

Chemistry databases

ChEMBLiCHEMBL1893
DrugBankiDB01472 4-Methoxyamphetamine
DB00386 Alseroxylon
DB00182 Amphetamine
DB00865 Benzphetamine
DB01089 Deserpidine
DB01576 Dextroamphetamine
DB01363 Ephedra
DB01364 Ephedrine
DB06706 Isometheptene
DB01577 Methamphetamine
DB01442 MMDA
DB00368 Norepinephrine
DB06714 Propylhexedrine
DB00206 Reserpine
DB04844 Tetrabenazine
GuidetoPHARMACOLOGYi1012

Polymorphism and mutation databases

BioMutaiSLC18A2
DMDMi1722742

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275141 – 514Synaptic vesicular amine transporterAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi84N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi117 ↔ 3241 Publication
Modified residuei511PhosphoserineBy similarity1
Modified residuei513PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ05940
PaxDbiQ05940
PeptideAtlasiQ05940
PRIDEiQ05940
ProteomicsDBi58362

PTM databases

iPTMnetiQ05940
PhosphoSitePlusiQ05940

Expressioni

Gene expression databases

BgeeiENSG00000165646
CleanExiHS_SLC18A2
GenevisibleiQ05940 HS

Organism-specific databases

HPAiHPA073224

Interactioni

Subunit structurei

Interacts with SLC6A3.By similarity

Protein-protein interaction databases

BioGridi112459, 1 interactor
IntActiQ05940, 9 interactors
STRINGi9606.ENSP00000298472

Chemistry databases

BindingDBiQ05940

Structurei

3D structure databases

ProteinModelPortaliQ05940
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3764 Eukaryota
ENOG410XW69 LUCA
GeneTreeiENSGT00390000003851
HOGENOMiHOG000018545
HOVERGENiHBG055082
InParanoidiQ05940
KOiK08155
OMAiRIGYQIP
OrthoDBiEOG091G0534
PhylomeDBiQ05940
TreeFamiTF313494

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05940-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSELALVR WLQESRRSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNATEI QTARPVHTAS ISDSFQSIFS YYDNSTMVTG NATRDLTLHQ
110 120 130 140 150
TATQHMVTNA SAVPSDCPSE DKDLLNENVQ VGLLFASKAT VQLITNPFIG
160 170 180 190 200
LLTNRIGYPI PIFAGFCIMF VSTIMFAFSS SYAFLLIARS LQGIGSSCSS
210 220 230 240 250
VAGMGMLASV YTDDEERGNV MGIALGGLAM GVLVGPPFGS VLYEFVGKTA
260 270 280 290 300
PFLVLAALVL LDGAIQLFVL QPSRVQPESQ KGTPLTTLLK DPYILIAAGS
310 320 330 340 350
ICFANMGIAM LEPALPIWMM ETMCSRKWQL GVAFLPASIS YLIGTNIFGI
360 370 380 390 400
LAHKMGRWLC ALLGMIIVGV SILCIPFAKN IYGLIAPNFG VGFAIGMVDS
410 420 430 440 450
SMMPIMGYLV DLRHVSVYGS VYAIADVAFC MGYAIGPSAG GAIAKAIGFP
460 470 480 490 500
WLMTIIGIID ILFAPLCFFL RSPPAKEEKM AILMDHNCPI KTKMYTQNNI
510
QSYPIGEDEE SESD
Length:514
Mass (Da):55,713
Last modified:October 1, 1996 - v2
Checksum:i42EA91A225485DB2
GO
Isoform 2 (identifier: Q05940-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     204-209: MGMLAS → RCGMPE
     210-514: Missing.

Note: No experimental confirmation available.
Show »
Length:209
Mass (Da):22,980
Checksum:iE5C6638E4F86FF66
GO

Sequence cautioni

The sequence BAB19009 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17R → H in CAA50489 (PubMed:8373557).Curated1
Sequence conflicti302C → S in AAA59877 (PubMed:8095030).Curated1
Sequence conflicti354K → T in AAA59877 (PubMed:8095030).Curated1
Sequence conflicti378A → P in AAA59877 (PubMed:8095030).Curated1
Sequence conflicti395I → N in AAA59877 (PubMed:8095030).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057151204 – 209MGMLAS → RCGMPE in isoform 2. 1 Publication6
Alternative sequenceiVSP_057152210 – 514Missing in isoform 2. 1 PublicationAdd BLAST305

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09118 mRNA Translation: AAA59877.1
L23205 mRNA Translation: AAA61290.1
L14269 mRNA Translation: AAA91853.1
X71354 mRNA Translation: CAA50489.1
AB044401 Genomic DNA Translation: BAB19009.1 Sequence problems.
AK314997 mRNA Translation: BAG37493.1
AL391988 Genomic DNA No translation available.
AL731557 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49428.1
CH471066 Genomic DNA Translation: EAW49429.1
BC030593 mRNA Translation: AAH30593.1
BC108927 mRNA Translation: AAI08928.1
BC108928 mRNA Translation: AAI08929.1
CCDSiCCDS7599.1 [Q05940-1]
PIRiA49368
S29810
RefSeqiNP_003045.2, NM_003054.4 [Q05940-1]
UniGeneiHs.596992

Genome annotation databases

EnsembliENST00000298472; ENSP00000298472; ENSG00000165646 [Q05940-1]
ENST00000644641; ENSP00000496339; ENSG00000165646 [Q05940-1]
GeneIDi6571
KEGGihsa:6571
UCSCiuc001ldd.3 human [Q05940-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVMAT2_HUMAN
AccessioniPrimary (citable) accession number: Q05940
Secondary accession number(s): B2RC96
, D3DRC4, Q15876, Q4G147, Q5VW49, Q9H3P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: July 18, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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