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UniProtKB - Q05927 (5NTD_BOVIN)
Protein
5'-nucleotidase
Gene
NT5E
Organism
Bos taurus (Bovine)
Status
Functioni
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Caution
The homodimer was initially thought to be disulfide bonded; however it is not the case.1 Publication
Catalytic activityi
- EC:3.1.3.5
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 36 | Zinc 1By similarity | 1 | |
Metal bindingi | 38 | Zinc 1By similarity | 1 | |
Metal bindingi | 85 | Zinc 1By similarity | 1 | |
Metal bindingi | 85 | Zinc 2By similarity | 1 | |
Metal bindingi | 117 | Zinc 2By similarity | 1 | |
Sitei | 118 | Transition state stabilizerBy similarity | 1 | |
Sitei | 121 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 220 | Zinc 2By similarity | 1 | |
Metal bindingi | 243 | Zinc 2By similarity | 1 | |
Binding sitei | 417 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- 5'-nucleotidase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- nucleotide catabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
SABIO-RKi | Q05927 |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-nucleotidase (EC:3.1.3.5)Short name: 5'-NT Alternative name(s): Ecto-5'-nucleotidase CD_antigen: CD73 |
Gene namesi | Name:NT5E Synonyms:NT5, NTE |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- anchored component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Add BLAST | 26 | |
ChainiPRO_0000000013 | 27 – 549 | 5'-nucleotidaseAdd BLAST | 523 | |
PropeptideiPRO_0000000014 | 550 – 574 | Removed in mature formBy similarityAdd BLAST | 25 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 51 ↔ 57 | By similarity | ||
Glycosylationi | 53 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 311 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 333 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 353 ↔ 358 | By similarity | ||
Disulfide bondi | 365 ↔ 387 | By similarity | ||
Glycosylationi | 403 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 476 ↔ 479 | By similarity | ||
Lipidationi | 549 | GPI-anchor amidated serineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, LipoproteinProteomic databases
PaxDbi | Q05927 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000054917 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 500 – 506 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Belongs to the 5'-nucleotidase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4419, Eukaryota |
InParanoidi | Q05927 |
OrthoDBi | 484599at2759 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit 3.90.780.10, 1 hit |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 1 hit |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 1 hit PF00149, Metallophos, 1 hit |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 1 hit SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 1 hit PS00786, 5_NUCLEOTIDASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q05927-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPGAARTPA LRILALGALL WPAARPWELT ILHTNDVHSR LEQTSEDSSK
60 70 80 90 100
CVNASRCVGG VARLATKVHQ IRRAEPHVLL LDAGDQYQGT IWFTVYKGTE
110 120 130 140 150
VAHFMNALGY DAMALGNHEF DNGVEGLIDP LLKEVNFPIL SANIKAKGPL
160 170 180 190 200
ASKISGLYSP YKILTVGDEV VGIVGYTSKE TPFLSNPGTN LVFEDEITAL
210 220 230 240 250
QPEVDKLKTL NVNKIIALGH SGFEVDKLIA QKVKGVDVVV GGHSNTFLYT
260 270 280 290 300
GNPPSKEVPA GQYPFIVTSD DGRKVPVVQA YAFGKYLGYL KVEFDEKGNV
310 320 330 340 350
VTSHGNPILL NSSIPEDPNI KADINKWRVK LDNYSTQELG KTIVYLDGTA
360 370 380 390 400
QSCRFRECNM GNLICDAMIN NNLRHPDEMS WNHVSMCILN GGGIRSPIDE
410 420 430 440 450
RNNGTITWEN LAAVLPFGGT FDLVQLKGST LKKAFEHSVH RYGQATGEFL
460 470 480 490 500
QVGGIHVVYD ISRNPGDRVV KLEVLCTQCR VPSYEPLRMD KVYKVILPSF
510 520 530 540 550
LVSGGDGFQM IKDEKIKHDS GDQDINVVSG YISKMKVLYP AVEGRIQFSA
560 570
GSHCCGSFSL IFLSVLAVII ILYQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | A → P in BAA03408 (PubMed:8340354).Curated | 1 | |
Sequence conflicti | 15 | A → P in AAB27698 (PubMed:8340354).Curated | 1 | |
Sequence conflicti | 111 – 112 | DA → ES in BAA03408 (PubMed:8340354).Curated | 2 | |
Sequence conflicti | 111 – 112 | DA → ES in AAB27698 (PubMed:8340354).Curated | 2 | |
Sequence conflicti | 436 – 438 | EHS → DDT in BAA03408 (PubMed:8340354).Curated | 3 | |
Sequence conflicti | 436 – 438 | EHS → DDT in AAB27698 (PubMed:8340354).Curated | 3 | |
Sequence conflicti | 445 | A → R in BAA03408 (PubMed:8340354).Curated | 1 | |
Sequence conflicti | 445 | A → R in AAB27698 (PubMed:8340354).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14541 mRNA Translation: BAA03408.1 S64302 mRNA Translation: AAB27698.1 BC114093 mRNA Translation: AAI14094.1 BT026240 mRNA Translation: ABG67079.1 |
PIRi | JX0269 |
RefSeqi | NP_776554.2, NM_174129.3 |
Genome annotation databases
GeneIDi | 281363 |
KEGGi | bta:281363 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14541 mRNA Translation: BAA03408.1 S64302 mRNA Translation: AAB27698.1 BC114093 mRNA Translation: AAI14094.1 BT026240 mRNA Translation: ABG67079.1 |
PIRi | JX0269 |
RefSeqi | NP_776554.2, NM_174129.3 |
3D structure databases
SMRi | Q05927 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000054917 |
Proteomic databases
PaxDbi | Q05927 |
Genome annotation databases
GeneIDi | 281363 |
KEGGi | bta:281363 |
Organism-specific databases
CTDi | 4907 |
Phylogenomic databases
eggNOGi | KOG4419, Eukaryota |
InParanoidi | Q05927 |
OrthoDBi | 484599at2759 |
Enzyme and pathway databases
SABIO-RKi | Q05927 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit 3.90.780.10, 1 hit |
InterProi | View protein in InterPro IPR008334, 5'-Nucleotdase_C IPR036907, 5'-Nucleotdase_C_sf IPR006146, 5'-Nucleotdase_CS IPR006179, 5_nucleotidase/apyrase IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like |
PANTHERi | PTHR11575, PTHR11575, 1 hit |
Pfami | View protein in Pfam PF02872, 5_nucleotid_C, 1 hit PF00149, Metallophos, 1 hit |
PRINTSi | PR01607, APYRASEFAMLY |
SUPFAMi | SSF55816, SSF55816, 1 hit SSF56300, SSF56300, 1 hit |
PROSITEi | View protein in PROSITE PS00785, 5_NUCLEOTIDASE_1, 1 hit PS00786, 5_NUCLEOTIDASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 5NTD_BOVIN | |
Accessioni | Q05927Primary (citable) accession number: Q05927 Secondary accession number(s): Q24K05 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | April 3, 2007 | |
Last modified: | September 29, 2021 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families