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Protein

GTP cyclohydrolase 1

Gene

Gch1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence.By similarity

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.By similarity

Enzyme regulationi

GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity. Zinc is required for catalytic activity. Inhibited by Mg2+.By similarity

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (Gch1)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87Involved in pterin ring bindingBy similarity1
Sitei96Involved in pterin ring bindingBy similarity1
Metal bindingi132ZincBy similarity1
Metal bindingi135ZincBy similarity1
Metal bindingi203ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Hydrolase
Biological processTetrahydrobiopterin biosynthesis
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.16 3474
ReactomeiR-MMU-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
UniPathwayiUPA00848; UER00151

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16By similarity)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:Gch1
Synonyms:Gch
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:95675 Gch1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000107181 – 11By similarityAdd BLAST11
ChainiPRO_000001071912 – 241GTP cyclohydrolase 1Add BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineCombined sources1
Modified residuei72PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ05915
PeptideAtlasiQ05915
PRIDEiQ05915

PTM databases

iPTMnetiQ05915
PhosphoSitePlusiQ05915

Expressioni

Gene expression databases

BgeeiENSMUSG00000037580
CleanExiMM_GCH1
ExpressionAtlasiQ05915 baseline and differential
GenevisibleiQ05915 MM

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers. Interacts with AHSA1 and GCHFR/GFRP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199864, 1 interactor
STRINGi10090.ENSMUSP00000087405

Structurei

3D structure databases

ProteinModelPortaliQ05915
SMRiQ05915
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

eggNOGiKOG2698 Eukaryota
COG0302 LUCA
GeneTreeiENSGT00390000013481
HOGENOMiHOG000221222
HOVERGENiHBG003136
InParanoidiQ05915
KOiK01495
OMAiPFMGKVH
OrthoDBiEOG091G0PCE
PhylomeDBiQ05915
TreeFamiTF105616

Family and domain databases

HAMAPiMF_00223 FolE, 1 hit
InterProiView protein in InterPro
IPR001474 GTP_CycHdrlase_I
IPR018234 GTP_CycHdrlase_I_CS
IPR020602 GTP_CycHdrlase_I_dom
PANTHERiPTHR11109 PTHR11109, 1 hit
PfamiView protein in Pfam
PF01227 GTP_cyclohydroI, 1 hit
TIGRFAMsiTIGR00063 folE, 1 hit
PROSITEiView protein in PROSITE
PS00859 GTP_CYCLOHYDROL_1_1, 1 hit
PS00860 GTP_CYCLOHYDROL_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKPRGVRCT NGFSERELPR PGASPPAEKS RPPEAKGAQP ADAWKAGRHR
60 70 80 90 100
SEEENQVNLP KLAAAYSSIL LSLGEDPQRQ GLLKTPWRAA TAMQYFTKGY
110 120 130 140 150
QETISDVLND AIFDEDHDEM VIVKDIDMFS MCEHHLVPFV GRVHIGYLPN
160 170 180 190 200
KQVLGLSKLA RIVEIYSRRL QVQERLTKQI AVAITEALQP AGVGVVIEAT
210 220 230 240
HMCMVMRGVQ KMNSKTVTST MLGVFREDPK TREEFLTLIR S
Length:241
Mass (Da):27,014
Last modified:February 1, 1994 - v1
Checksum:iB4366F102B4A6FE6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54E → D in AAC60677 (PubMed:8304101).Curated1
Sequence conflicti56Q → E in AAC60677 (PubMed:8304101).Curated1
Sequence conflicti235F → S in AAC60677 (PubMed:8304101).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09737 mRNA Translation: AAA37757.1
BC005643 mRNA Translation: AAH05643.1
BC069921 mRNA Translation: AAH69921.1
S71373 mRNA Translation: AAC60677.2
CCDSiCCDS26984.1
PIRiJQ1934
RefSeqiNP_032128.1, NM_008102.3
UniGeneiMm.10651

Genome annotation databases

EnsembliENSMUST00000089959; ENSMUSP00000087405; ENSMUSG00000037580
GeneIDi14528
KEGGimmu:14528
UCSCiuc007tht.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGCH1_MOUSE
AccessioniPrimary (citable) accession number: Q05915
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 20, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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