UniProtKB - Q05900 (RU1C_YEAST)
Protein
U1 small nuclear ribonucleoprotein C
Gene
YHC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. YHC1/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.3 Publications
Miscellaneous
Present with 2140 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 4 – 36 | Matrin-typeUniRule annotationAdd BLAST | 33 |
GO - Molecular functioni
- mRNA binding Source: SGD
- pre-mRNA 5'-splice site binding Source: SGD
- U1 snRNA binding Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- mRNA 5'-splice site recognition Source: SGD
- mRNA splicing, via spliceosome Source: SGD
- positive regulation of RNA binding Source: SGD
- spliceosomal snRNP assembly Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: U1 small nuclear ribonucleoprotein CUniRule annotationShort name: U1 snRNP CUniRule annotation Short name: U1-CUniRule annotation Short name: U1CUniRule annotation |
Gene namesi | Name:YHC1UniRule annotation Ordered Locus Names:YLR298C ORF Names:L8003.21 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YLR298C |
SGDi | S000004289, YHC1 |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation1 Publication
Nucleus
- commitment complex Source: SGD
- U1 snRNP Source: SGD
- U2-type prespliceosome Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 13 | L → A, D, E, F, G, H, K, P, R, S, T, W or Y: Gives rise to unstable commitment complexes. 2 Publications | 1 | |
Mutagenesisi | 13 | L → C, I, M, N, Q or V: No effect. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097528 | 1 – 231 | U1 small nuclear ribonucleoprotein CAdd BLAST | 231 |
Proteomic databases
MaxQBi | Q05900 |
PaxDbi | Q05900 |
PRIDEi | Q05900 |
Interactioni
Subunit structurei
U1 snRNP is composed of the 7 core Sm proteins SMB1, SMD1, SMD2, SMD3, SME1, SMX3 and SMX2 (Sm proteins B, D1, D2, D3, E, F and G, respectively) that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 10 U1 snRNP-specific proteins SNP1/U1-70K, MUD1/U1-A, YHC1/U1-C, LUC7, NAM8, PRP39, PRP40, PRP42, SNU56 and SNU71. YHC1/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 31562, 768 interactors |
ComplexPortali | CPX-23, U1 snRNP |
DIPi | DIP-6661N |
IntActi | Q05900, 19 interactors |
MINTi | Q05900 |
STRINGi | 4932.YLR298C |
Miscellaneous databases
RNActi | Q05900, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q05900 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the U1 small nuclear ribonucleoprotein C family.UniRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 4 – 36 | Matrin-typeUniRule annotationAdd BLAST | 33 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3454, Eukaryota |
HOGENOMi | CLU_104749_0_0_1 |
InParanoidi | Q05900 |
OMAi | ADYYRNK |
Family and domain databases
HAMAPi | MF_03153, U1_C, 1 hit |
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR013085, U1-CZ_Znf_C2H2 IPR017340, U1_snRNP-C IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR31148, PTHR31148, 1 hit |
Pfami | View protein in Pfam PF06220, zf-U1, 1 hit |
SMARTi | View protein in SMART SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50171, ZF_MATRIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q05900-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRYYCEYCH SYLTHDTLSV RKSHLVGKNH LRITADYYRN KARDIINKHN
60 70 80 90 100
HKRRHIGKRG RKERENSSQN ETLKVTCLSN KEKRHIMHVK KMNQKELAQT
110 120 130 140 150
SIDTLKLLYD GSPGYSKVFV DANRFDIGDL VKASKLPQRA NEKSAHHSFK
160 170 180 190 200
QTSRSRDETC ESNPFPRLNN PKKLEPPKIL SQWSNTIPKT SIFYSVDILQ
210 220 230
TTIKESKKRM HSDGIRKPSS ANGYKRRRYG N
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17243 Genomic DNA Translation: AAB67343.1 AY692761 Genomic DNA Translation: AAT92780.1 BK006945 Genomic DNA Translation: DAA09608.1 |
PIRi | S50382 |
RefSeqi | NP_013401.1, NM_001182186.1 |
Genome annotation databases
EnsemblFungii | YLR298C_mRNA; YLR298C; YLR298C |
GeneIDi | 851005 |
KEGGi | sce:YLR298C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17243 Genomic DNA Translation: AAB67343.1 AY692761 Genomic DNA Translation: AAT92780.1 BK006945 Genomic DNA Translation: DAA09608.1 |
PIRi | S50382 |
RefSeqi | NP_013401.1, NM_001182186.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ZWN | electron microscopy | 3.30 | R | 1-231 | [»] | |
6G90 | electron microscopy | 4.00 | C | 1-231 | [»] | |
6N7P | electron microscopy | 3.60 | B | 1-231 | [»] | |
6N7R | electron microscopy | 3.20 | B | 1-231 | [»] | |
6N7X | electron microscopy | 3.60 | B | 1-231 | [»] | |
SMRi | Q05900 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31562, 768 interactors |
ComplexPortali | CPX-23, U1 snRNP |
DIPi | DIP-6661N |
IntActi | Q05900, 19 interactors |
MINTi | Q05900 |
STRINGi | 4932.YLR298C |
Proteomic databases
MaxQBi | Q05900 |
PaxDbi | Q05900 |
PRIDEi | Q05900 |
Genome annotation databases
EnsemblFungii | YLR298C_mRNA; YLR298C; YLR298C |
GeneIDi | 851005 |
KEGGi | sce:YLR298C |
Organism-specific databases
EuPathDBi | FungiDB:YLR298C |
SGDi | S000004289, YHC1 |
Phylogenomic databases
eggNOGi | KOG3454, Eukaryota |
HOGENOMi | CLU_104749_0_0_1 |
InParanoidi | Q05900 |
OMAi | ADYYRNK |
Miscellaneous databases
ChiTaRSi | YHC1, yeast |
PROi | PR:Q05900 |
RNActi | Q05900, protein |
Family and domain databases
HAMAPi | MF_03153, U1_C, 1 hit |
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR013085, U1-CZ_Znf_C2H2 IPR017340, U1_snRNP-C IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR31148, PTHR31148, 1 hit |
Pfami | View protein in Pfam PF06220, zf-U1, 1 hit |
SMARTi | View protein in SMART SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50171, ZF_MATRIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RU1C_YEAST | |
Accessioni | Q05900Primary (citable) accession number: Q05900 Secondary accession number(s): D6VYU2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | November 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD