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Protein

Thrombospondin-3

Gene

Thbs3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.

GO - Molecular functioni

GO - Biological processi

  • bone trabecula formation Source: MGI
  • cell adhesion Source: UniProtKB-KW
  • growth plate cartilage development Source: MGI
  • ossification involved in bone maturation Source: MGI

Keywordsi

Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-186797 Signaling by PDGF

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-3
Gene namesi
Name:Thbs3
Synonyms:Tsp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:98739 Thbs3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000003585022 – 956Thrombospondin-3Add BLAST935

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi266InterchainCurated
Disulfide bondi269InterchainCurated
Disulfide bondi278 ↔ 289PROSITE-ProRule annotation
Disulfide bondi283 ↔ 300PROSITE-ProRule annotation
Disulfide bondi303 ↔ 314PROSITE-ProRule annotation
Glycosylationi310N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi320 ↔ 332PROSITE-ProRule annotation
Disulfide bondi326 ↔ 341PROSITE-ProRule annotation
Disulfide bondi344 ↔ 368PROSITE-ProRule annotation
Disulfide bondi374 ↔ 388PROSITE-ProRule annotation
Disulfide bondi382 ↔ 397PROSITE-ProRule annotation
Disulfide bondi400 ↔ 412PROSITE-ProRule annotation
Glycosylationi407N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi418 ↔ 432PROSITE-ProRule annotation
Disulfide bondi426 ↔ 442PROSITE-ProRule annotation
Disulfide bondi444 ↔ 455PROSITE-ProRule annotation
Disulfide bondi471 ↔ 478PROSITE-ProRule annotation
Disulfide bondi483 ↔ 503PROSITE-ProRule annotation
Disulfide bondi519 ↔ 539PROSITE-ProRule annotation
Disulfide bondi542 ↔ 562PROSITE-ProRule annotation
Disulfide bondi578 ↔ 598PROSITE-ProRule annotation
Disulfide bondi601 ↔ 621PROSITE-ProRule annotation
Disulfide bondi639 ↔ 659PROSITE-ProRule annotation
Glycosylationi644N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi679 ↔ 699PROSITE-ProRule annotation
Disulfide bondi715 ↔ 936PROSITE-ProRule annotation
Glycosylationi937N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ05895
PaxDbiQ05895
PRIDEiQ05895

PTM databases

PhosphoSitePlusiQ05895

Expressioni

Tissue specificityi

Brain, lung and cartilage.

Gene expression databases

BgeeiENSMUSG00000028047 Expressed in 226 organ(s), highest expression level in humerus cartilage element
CleanExiMM_THBS3
ExpressionAtlasiQ05895 baseline and differential
GenevisibleiQ05895 MM

Interactioni

Subunit structurei

Oligomer; disulfide-linked.

Protein-protein interaction databases

ComplexPortaliCPX-3024 Thrombospondin 3 complex
STRINGi10090.ENSMUSP00000029682

Structurei

3D structure databases

ProteinModelPortaliQ05895
SMRiQ05895
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 193Laminin G-likeAdd BLAST172
Domaini316 – 354EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini370 – 410EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini414 – 456EGF-like 3PROSITE-ProRule annotationAdd BLAST43
Repeati457 – 491TSP type-3 1Add BLAST35
Repeati492 – 527TSP type-3 2Add BLAST36
Repeati528 – 550TSP type-3 3Add BLAST23
Repeati551 – 586TSP type-3 4Add BLAST36
Repeati587 – 609TSP type-3 5Add BLAST23
Repeati610 – 647TSP type-3 6Add BLAST38
Repeati648 – 687TSP type-3 7Add BLAST40
Repeati688 – 723TSP type-3 8Add BLAST36
Domaini727 – 941TSP C-terminalPROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the thrombospondin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ Eukaryota
ENOG410XQKE LUCA
GeneTreeiENSGT00930000150838
HOGENOMiHOG000007542
HOVERGENiHBG000636
InParanoidiQ05895
KOiK04659
OMAiESMKMIL
OrthoDBiEOG091G00TV
TreeFamiTF324917

Family and domain databases

Gene3Di4.10.1080.10, 3 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR018097 EGF_Ca-bd_CS
IPR001791 Laminin_G
IPR024665 Thbs/COMP_coiled-coil
IPR037349 Thrombospondin
IPR028507 Thrombospondin-3
IPR003367 Thrombospondin_3-like_rpt
IPR017897 Thrombospondin_3_rpt
IPR008859 Thrombospondin_C
IPR028974 TSP_type-3_rpt
PANTHERiPTHR10199 PTHR10199, 1 hit
PTHR10199:SF89 PTHR10199:SF89, 1 hit
PfamiView protein in Pfam
PF11598 COMP, 1 hit
PF07645 EGF_CA, 2 hits
PF02412 TSP_3, 6 hits
PF05735 TSP_C, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 4 hits
SM00179 EGF_CA, 2 hits
SM00210 TSPN, 1 hit
SUPFAMiSSF103647 SSF103647, 3 hits
SSF49899 SSF49899, 2 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 3 hits
PS01187 EGF_CA, 2 hits
PS51234 TSP3, 8 hits
PS51236 TSP_CTER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q05895-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEKPELWGVL ALLLLCSYTC GSQDLQVIDL LTVGESRQMV AVAEKIRTAL
60 70 80 90 100
LTAGDIYLLS TFRLPPKQGG VLFGLYSRQD NTRWLEASVV GKINKVLVRY
110 120 130 140 150
QREDGKVHAV NLQQAGLADG RTHTALLRLR GPSRPSPGLQ LYVDCKLGDQ
160 170 180 190 200
HAGLPALAPI PPAEVSGLEI RTGQKAYLRM QGFVESMKII LGGSMARVGA
210 220 230 240 250
LSECPFQGDD SIHNAVTSAL QSILGEQTKA LVTQLTLFNQ ILVELRDDIR
260 270 280 290 300
DQVKEMSLIR NTIMECQVCG FHEQRSHCSP SPCFRGVDCM EVYEYPGYRC
310 320 330 340 350
GPCPPGLQGN GTHCDDINEC AHADPCFPGS SCINTMPGFH CEACPPGYKG
360 370 380 390 400
TRVSGVGIDY ARASKQVCND IDECNDGNNG GCDPNSICTN TVGSFKCGPC
410 420 430 440 450
RLGFLGNQSQ GCVPARTCHS PAHSPCHIHA HCLFERNGAV SCQCNVGWAG
460 470 480 490 500
NGNVCGPDTD IDGYPDQALP CMDNNKHCKQ DNCLLTPNSG QEDADNDGVG
510 520 530 540 550
DQCDDDADGD GIKNVEDNCR LFPNKDQQNS DTDSFGDACD NCPNVPNNDQ
560 570 580 590 600
KDTDGNGEGD ACDNDVDGDG IPNGLDNCPK VPNPLQTDRD EDGVGDACDS
610 620 630 640 650
CPEMSNPTQT DADSDLVGDV CDTNEDSDGD GHQDTKDNCP QLPNSSQLDS
660 670 680 690 700
DNDGLGDECD GDDDNDGVPD YIPPGPDNCR LVPNPNQKDS DGNGVGDVCE
710 720 730 740 750
DDFDNDAVVD PLDVCPESAE VTLTDFRAYQ TVILDPEGDA QIDPNWVVLN
760 770 780 790 800
QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTVTDDD YAGFLFSYQD
810 820 830 840 850
SGRFYVVMWK QTEQTYWQAT PFRAVAQPGL QLKAVTSISG PGEHLRNALW
860 870 880 890 900
HTGHTPDQVR LLWTDPRNVG WRDKTSYRWR LLHRPQVGYI RVKLYEGPQL
910 920 930 940 950
VADSGVIIDT SMRGGRLGVF CFSQENIIWS NLQYRCNDTV PEDFEPFRRQ

LLQGRV
Length:956
Mass (Da):104,119
Last modified:July 27, 2011 - v2
Checksum:i4FAC268D11C83CAA
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PWF0E9PWF0_MOUSE
Thrombospondin-3
Thbs3
919Annotation score:
D3Z6N1D3Z6N1_MOUSE
Thrombospondin-3
Thbs3
131Annotation score:
F6W6J3F6W6J3_MOUSE
Thrombospondin-3
Thbs3
104Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti433L → F in AAA40433 (PubMed:8288588).Curated1
Sequence conflicti563D → N in AAA40497 (PubMed:8468055).Curated1
Sequence conflicti563D → N in AAA40430 (PubMed:1601886).Curated1
Sequence conflicti720E → G in AAA40497 (PubMed:8468055).Curated1
Sequence conflicti720E → G in AAA40430 (PubMed:1601886).Curated1
Sequence conflicti871W → L in AAA40497 (PubMed:8468055).Curated1
Sequence conflicti871W → L in AAA40430 (PubMed:1601886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04302 mRNA Translation: AAA40497.1
L24434 mRNA Translation: AAA40433.1
CH466547 Genomic DNA Translation: EDL15225.1
BC053023 mRNA Translation: AAH53023.1
M86620
, M86611, M86612, M86613, M86614, M86615, M86616, M86617, M86618, M86619 Genomic DNA Translation: AAA40430.1
U16175 Genomic DNA Translation: AAA98537.1
U66257 Genomic DNA Translation: AAC52819.1
CCDSiCCDS17495.1
PIRiA46016
RefSeqiNP_038719.2, NM_013691.2
UniGeneiMm.2114

Genome annotation databases

EnsembliENSMUST00000029682; ENSMUSP00000029682; ENSMUSG00000028047
GeneIDi21827
KEGGimmu:21827
UCSCiuc008pyg.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04302 mRNA Translation: AAA40497.1
L24434 mRNA Translation: AAA40433.1
CH466547 Genomic DNA Translation: EDL15225.1
BC053023 mRNA Translation: AAH53023.1
M86620
, M86611, M86612, M86613, M86614, M86615, M86616, M86617, M86618, M86619 Genomic DNA Translation: AAA40430.1
U16175 Genomic DNA Translation: AAA98537.1
U66257 Genomic DNA Translation: AAC52819.1
CCDSiCCDS17495.1
PIRiA46016
RefSeqiNP_038719.2, NM_013691.2
UniGeneiMm.2114

3D structure databases

ProteinModelPortaliQ05895
SMRiQ05895
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-3024 Thrombospondin 3 complex
STRINGi10090.ENSMUSP00000029682

PTM databases

PhosphoSitePlusiQ05895

Proteomic databases

MaxQBiQ05895
PaxDbiQ05895
PRIDEiQ05895

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029682; ENSMUSP00000029682; ENSMUSG00000028047
GeneIDi21827
KEGGimmu:21827
UCSCiuc008pyg.2 mouse

Organism-specific databases

CTDi7059
MGIiMGI:98739 Thbs3

Phylogenomic databases

eggNOGiENOG410IFQQ Eukaryota
ENOG410XQKE LUCA
GeneTreeiENSGT00930000150838
HOGENOMiHOG000007542
HOVERGENiHBG000636
InParanoidiQ05895
KOiK04659
OMAiESMKMIL
OrthoDBiEOG091G00TV
TreeFamiTF324917

Enzyme and pathway databases

ReactomeiR-MMU-186797 Signaling by PDGF

Miscellaneous databases

PROiPR:Q05895
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028047 Expressed in 226 organ(s), highest expression level in humerus cartilage element
CleanExiMM_THBS3
ExpressionAtlasiQ05895 baseline and differential
GenevisibleiQ05895 MM

Family and domain databases

Gene3Di4.10.1080.10, 3 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR018097 EGF_Ca-bd_CS
IPR001791 Laminin_G
IPR024665 Thbs/COMP_coiled-coil
IPR037349 Thrombospondin
IPR028507 Thrombospondin-3
IPR003367 Thrombospondin_3-like_rpt
IPR017897 Thrombospondin_3_rpt
IPR008859 Thrombospondin_C
IPR028974 TSP_type-3_rpt
PANTHERiPTHR10199 PTHR10199, 1 hit
PTHR10199:SF89 PTHR10199:SF89, 1 hit
PfamiView protein in Pfam
PF11598 COMP, 1 hit
PF07645 EGF_CA, 2 hits
PF02412 TSP_3, 6 hits
PF05735 TSP_C, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 4 hits
SM00179 EGF_CA, 2 hits
SM00210 TSPN, 1 hit
SUPFAMiSSF103647 SSF103647, 3 hits
SSF49899 SSF49899, 2 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 3 hits
PS01187 EGF_CA, 2 hits
PS51234 TSP3, 8 hits
PS51236 TSP_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTSP3_MOUSE
AccessioniPrimary (citable) accession number: Q05895
Secondary accession number(s): Q6LCE0, Q7TN15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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