Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-amylase

Gene

amy

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi182CalciumBy similarity1
Metal bindingi281CalciumBy similarity1
Active sitei312NucleophileBy similarity1
Metal bindingi316Calcium; via carbonyl oxygenBy similarity1
Active sitei346Proton donorBy similarity1
Sitei417Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiCBM41 Carbohydrate-Binding Module Family 41
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amy
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000000134534 – 919Alpha-amylaseAdd BLAST886

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ05884
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd10315 CBM41_pullulanase, 2 hits
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR013784 Carb-bd-like_fold
IPR005323 CBM41_pullulanase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF03714 PUD, 2 hits
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF49452 SSF49452, 2 hits
SSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05884-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPATRRTARV RRVAAVTVTA LAAALLPPLA ARADTPPAPP SDAKLAKTAA
60 70 80 90 100
RHDLTREQFY FSCRTLRQRG RRERPRRLTG TRLTTGYDPT DKGFYQGGDL
110 120 130 140 150
KGLTEKLDYI KGLGTTSIWM APIFKNQPVQ GTGKDASAGY HGYWITDFTQ
160 170 180 190 200
VDPHFGTNKD LKNLISKAHA KGMKVFFDVI TNHTADVVDY EEKSYDYLSK
210 220 230 240 250
GAFPYLTKDG QPFDDADYAD GERRFPRVDS GSFPRTPTVP TAKKNLKVPS
260 270 280 290 300
WLNDPAMYHN RGDSTWAGES ATYGDFNGLD DLWTERPEVV GGMEKIYQRW
310 320 330 340 350
VEDFAIDGFR IDTVKHVDME FWTQWATALD AYAAKKGRDD FFMFGEVYSA
360 370 380 390 400
DTSVTAPYVT QGRLDSTLDF PFQDAARAYA SQGGSARKLA AVFGDDYKYT
410 420 430 440 450
TDKANAYEQV TFLGNHDMGR IGTFLKQDAP EAGDAELLKK DRLANELMFL
460 470 480 490 500
SRGNPVIYYG DEQGFTGAGG DKDARQPMFA SRTADYLDDD QLGTDRTHAE
510 520 530 540 550
AAYDTSAPLY RQISALAELR KANPALADGV QTERYAADGA GIYAFSRTDA
560 570 580 590 600
KTGTEYVVAF NNAGTEPSAA FATGSAGMTF RGLYGTDATV KSGADSKVTV
610 620 630 640 650
TVPARSAVVL KAAGRLAAPA AEPTISLHAP DPGATGTVEL SADVAGGQLN
660 670 680 690 700
RVVFAAQTGD GKWRTLGTAD HAPYKVTHTV DADTPAGTAL RYKAVVVDSA
710 720 730 740 750
GRTASGRLHH RHPARRGGAH RRLPGPRGRP LQARRRELRR LGPVRLGRPR
760 770 780 790 800
RREAHHLARH PPLHRPGRLR AFAYVKLKPG ASTVGFLVID KDGNKDVAAD
810 820 830 840 850
RTIDVTETGE VWIEQGEEQL VTERPEYPAQ DTTKAVLHYK RADGNYDGWG
860 870 880 890 900
LHVWGDAANP TDWAKPLQPV RTDPYGAVFE VPLTDGASSL SYMVHKGDEK
910
DLPTDQAWTS RPTATRCGC
Length:919
Mass (Da):100,642
Last modified:February 1, 1995 - v1
Checksum:i035B59FB80F0BD10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70255 Genomic DNA Translation: CAA49759.1
PIRiS28179

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70255 Genomic DNA Translation: CAA49759.1
PIRiS28179

3D structure databases

ProteinModelPortaliQ05884
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM41 Carbohydrate-Binding Module Family 41
GH13 Glycoside Hydrolase Family 13

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd10315 CBM41_pullulanase, 2 hits
Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR013784 Carb-bd-like_fold
IPR005323 CBM41_pullulanase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF03714 PUD, 2 hits
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF49452 SSF49452, 2 hits
SSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY_STRLI
AccessioniPrimary (citable) accession number: Q05884
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again