UniProtKB - Q05861 (A85A_MYCLE)
Diacylglycerol acyltransferase/mycolyltransferase Ag85A
fbpA
Functioni
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA (By similarity).
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 168 | NucleophileBy similarity | 1 | |
Binding sitei | 168 | SubstrateBy similarity | 1 | |
Binding sitei | 196 | SubstrateBy similarity | 1 | |
Active sitei | 272 | By similarity | 1 | |
Binding sitei | 281 | SubstrateBy similarity | 1 | |
Active sitei | 304 | By similarity | 1 |
GO - Molecular functioni
- diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
- trehalose O-mycolyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Acyltransferase, Transferase |
Protein family/group databases
ESTHERi | mycle-a85a, A85-Mycolyl-transferase |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fbpA Ordered Locus Names:ML0097 |
Organismi | Mycobacterium leprae (strain TN) |
Taxonomic identifieri | 272631 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › |
Proteomesi |
|
Organism-specific databases
Lepromai | ML0097 |
Subcellular locationi
Extracellular region or secreted
- extracellular region Source: UniProtKB-KW
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell wall, Cytoplasm, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 42 | Sequence analysisAdd BLAST | 42 | |
ChainiPRO_0000000213 | 43 – 330 | Diacylglycerol acyltransferase/mycolyltransferase Ag85AAdd BLAST | 288 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 129 ↔ 134 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PRIDEi | Q05861 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 272631.ML0097 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 84 – 85 | Substrate bindingBy similarity | 2 | |
Regioni | 100 – 110 | Fibronectin-bindingAdd BLAST | 11 | |
Regioni | 274 – 277 | Substrate bindingBy similarity | 4 | |
Regioni | 304 – 306 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0627, Bacteria |
HOGENOMi | CLU_026624_3_1_11 |
OMAi | AWARNDP |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MKFVDRFRGA VAGMLRRLVV EAMGVALLSA LIGVVGSAPA EAFSRPGLPV
60 70 80 90 100
EYLQVPSPSM GRDIKVQFQN GGANSPALYL LDGLRAQDDF SGWDINTTAF
110 120 130 140 150
EWYYQSGISV VMPVGGQSSF YSDWYSPACG KAGCQTYKWE TFLTSELPQY
160 170 180 190 200
LQSNKQIKPT GSAAVGLSMA GLSALTLAIY HPDQFIYVGS MSGLLDPSNA
210 220 230 240 250
MGPSLIGLAM GDAGGYKAAD MWGPSTDPAW KRNDPTVNVG TLIANNTRIW
260 270 280 290 300
MYCGNGKPTE LGGNNLPAKL LEGLVRTSNI KFQDGYNAGG GHNAVFNFPD
310 320 330
SGTHSWEYWG EQLNDMKPDL QQYLGATPGA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 149 | Q → E in BAA07864 (PubMed:7829901).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D43841 Genomic DNA Translation: BAA07864.1 M90648 Genomic DNA Translation: AAA91864.1 AL583917 Genomic DNA Translation: CAC29605.1 Z21950 Genomic DNA Translation: CAA79948.1 |
PIRi | A86921 S32107 |
RefSeqi | NP_301195.1, NC_002677.1 WP_010907520.1, NC_002677.1 |
Genome annotation databases
EnsemblBacteriai | CAC29605; CAC29605; CAC29605 |
KEGGi | mle:ML0097 |
PATRICi | fig|272631.5.peg.151 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D43841 Genomic DNA Translation: BAA07864.1 M90648 Genomic DNA Translation: AAA91864.1 AL583917 Genomic DNA Translation: CAC29605.1 Z21950 Genomic DNA Translation: CAA79948.1 |
PIRi | A86921 S32107 |
RefSeqi | NP_301195.1, NC_002677.1 WP_010907520.1, NC_002677.1 |
3D structure databases
SMRi | Q05861 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 272631.ML0097 |
Protein family/group databases
ESTHERi | mycle-a85a, A85-Mycolyl-transferase |
Proteomic databases
PRIDEi | Q05861 |
Genome annotation databases
EnsemblBacteriai | CAC29605; CAC29605; CAC29605 |
KEGGi | mle:ML0097 |
PATRICi | fig|272631.5.peg.151 |
Organism-specific databases
Lepromai | ML0097 |
Phylogenomic databases
eggNOGi | COG0627, Bacteria |
HOGENOMi | CLU_026624_3_1_11 |
OMAi | AWARNDP |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A85A_MYCLE | |
Accessioni | Q05861Primary (citable) accession number: Q05861 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 114 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families