UniProtKB - Q05860 (FMN1_MOUSE)
Protein
Formin-1
Gene
Fmn1
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in the formation of adherens junction and the polymerization of linear actin cables.2 Publications
Miscellaneous
Was originally thought to play a role in limb bud development based on the fact that limb deformity (ld) mutants are associated with Fmn1 gene disruption. However, PubMed:15198975 shows that limb deformity mutations rather affects Grem1 cis-regulatory regions localized in Fmn1 gene and that loss of Grem1 (gremlin-1) expression is the cause of limb malformations.
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- microtubule binding Source: MGI
- SH3 domain binding Source: MGI
GO - Biological processi
- actin nucleation Source: InterPro
- dendrite morphogenesis Source: MGI
- forelimb morphogenesis Source: MGI
- gene expression Source: MGI
- hindlimb morphogenesis Source: MGI
- kidney development Source: MGI
- limb development Source: MGI
- positive regulation of actin filament polymerization Source: MGI
- positive regulation of actin nucleation Source: MGI
- positive regulation of focal adhesion assembly Source: MGI
- skeletal system morphogenesis Source: MGI
- ureteric bud invasion Source: MGI
Keywordsi
Molecular function | Actin-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Formin-1Alternative name(s): Limb deformity protein |
Gene namesi | Name:Fmn1 Synonyms:Fmn, Ld |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101815, Fmn1 |
Subcellular locationi
Cytoplasm and Cytosol
Nucleus
Plasma membrane
Other locations
Note: Localization to the adherens junctions is alpha-catenin-dependent. Also localizes to F-actin bundles originating from adherens junctions.
Cytoskeleton
Note: Isoform 2 localizes to microtubules.
Cytoskeleton
- actin filament Source: InterPro
- microtubule cytoskeleton Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- adherens junction Source: UniProtKB-SubCell
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194886 | 1 – 1466 | Formin-1Add BLAST | 1466 |
Post-translational modificationi
Phosphorylated on serine and possibly threonine residues.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q05860 |
PaxDbi | Q05860 |
PeptideAtlasi | Q05860 |
PRIDEi | Q05860 |
ProteomicsDBi | 267599 [Q05860-1] 267600 [Q05860-2] 267601 [Q05860-3] 267602 [Q05860-4] 267603 [Q05860-5] 267604 [Q05860-6] |
PTM databases
iPTMneti | Q05860 |
PhosphoSitePlusi | Q05860 |
Expressioni
Tissue specificityi
It is present in the adult kidney, testis, limb, ovary, brain, small intestine, salivary gland and harderian gland. Isoforms 1, 2 and 5 are detected in skin and keratinocytes. Isoform 5 is found throughout the embryo.2 Publications
Developmental stagei
It is present throughout the embryo. In the developing limb bud, the protein is expressed in the apical ectodermal ridge and the mesenchymal compartment, predominantly in the posterior region. During kidney morphogenesis, expression is initially restricted to the epithelial compartment of the pronephros and mesonephros. Isoform 5 is found in the apical ectodermal ridge and the mesenchymal compartment of the developing limb bud.
Gene expression databases
Bgeei | ENSMUSG00000044042, Expressed in substantia nigra and 219 other tissues |
ExpressionAtlasi | Q05860, baseline and differential |
Genevisiblei | Q05860, MM |
Interactioni
Subunit structurei
Interacts with alpha-catenin and may interact with tubulin.
2 PublicationsGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- microtubule binding Source: MGI
- SH3 domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199711, 13 interactors |
DIPi | DIP-646N |
ELMi | Q05860 |
IntActi | Q05860, 1 interactor |
STRINGi | 10090.ENSMUSP00000099606 |
Miscellaneous databases
RNActi | Q05860, protein |
Structurei
3D structure databases
SMRi | Q05860 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q05860 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 870 – 970 | FH1Add BLAST | 101 | |
Domaini | 983 – 1435 | FH2PROSITE-ProRule annotationAdd BLAST | 453 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 624 | Microtubule-bindingAdd BLAST | 624 | |
Regioni | 458 – 842 | Mediates interaction with alpha-cateninAdd BLAST | 385 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 722 – 786 | Sequence analysisAdd BLAST | 65 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 870 – 995 | Pro-richAdd BLAST | 126 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1922, Eukaryota |
GeneTreei | ENSGT00940000154289 |
HOGENOMi | CLU_271027_0_0_1 |
InParanoidi | Q05860 |
OMAi | DKMLMDE |
OrthoDBi | 249504at2759 |
PhylomeDBi | Q05860 |
TreeFami | TF351317 |
Family and domain databases
Gene3Di | 1.20.58.2220, 2 hits |
InterProi | View protein in InterPro IPR015425, FH2_Formin IPR042201, FH2_Formin_sf IPR001265, Formin_Cappuccino_subfam |
Pfami | View protein in Pfam PF02181, FH2, 1 hit |
PRINTSi | PR00828, FORMIN |
SMARTi | View protein in SMART SM00498, FH2, 1 hit |
PROSITEi | View protein in PROSITE PS51444, FH2, 1 hit |
s (6+)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q05860-1) [UniParc]FASTAAdd to basket
Also known as: IA
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGTHCTLQL HNPIAELCYI SFYLPKGEVR GFSYKGTVTL DRSNNAFHNC
60 70 80 90 100
YQVREGPDIT SLSQQPNEHP GDIFFKQTPT KNILTELYKL TAEKERLLDS
110 120 130 140 150
LLRSDNILGV SMGSQEGKLQ ELSVILATGD EYFQSAGNWR RELPVSSLIR
160 170 180 190 200
RSTQENKKPR RSGRRRESPE ELRQKRTRRK GRGCQESAFQ MGKDQVCSSS
210 220 230 240 250
SLSFRARPNL RLLEERGNLV PRGTLTSSLR RRESCPANIL RTPDADLAFG
260 270 280 290 300
NSGRTSEDTD LEGPLSPDSS PTEVGDADVG GQLKSSHQQE PPQPNVSESH
310 320 330 340 350
GKHAGAERWS SRTRKSKSLE RTCSKKPVSK VVAKIQEPSA PVKRIVRAHH
360 370 380 390 400
DGKGRVAYGP ETQTEFIPKA DFLTLPGGET ETHSSGRLEE EQPGIKSLRS
410 420 430 440 450
SAPERASITK EPASTEAAVN KVLRKVIESE KLDEATEGKR LGFSLNTRAT
460 470 480 490 500
HTFPETRSQR KAGLPQSGHK FLLLDLPHTV GPDSPQPKCD EKKPTPQVPT
510 520 530 540 550
ALGMVFNNSS PQSSAHKRLS PVPSPLSPRC PSPQQHHRIL LLPPLPSEGE
560 570 580 590 600
VVFNEYPSRK NDVSSGFPSA DTLEPSSTTK VTETKGASPT SLRASQTWLV
610 620 630 640 650
SEEASEKGLG PEKITAPPQH QLPPGIASEG FPCDNFKEQT AKDLPNKDGG
660 670 680 690 700
VWVPGYRAGP PCPFLLHEEK EKTSRSELYL DLNPDQSPTE QDDRTPGRLQ
710 720 730 740 750
AVWPPPKTKD TEEKVGLKYT EAEYQAAILH LKREHKEEIE TLQAQFELKT
760 770 780 790 800
FHIRGEHALV TARLEEAIEN LKQQLGKRRE GCEEMRDVCI STDDDCSPKA
810 820 830 840 850
FRNVCIQTDR ETFLKPCDAE SKATRSSQIV PKKLTISLTQ LSPSKDSKDI
860 870 880 890 900
HAPFQTREGT SSSSQHKISP PAPPTPPPLP PPLIPPPPPL PPGLGPLPPA
910 920 930 940 950
PPIPPVCPVS PPPPPPPPPP TPVPPSDGPP PPPPPPPPLP NVLALPNSGG
960 970 980 990 1000
PPPPPPPPPP GLAPPPPPGL SFGLSSSSSQ YPRKPAIEPS CPMKPLYWTR
1010 1020 1030 1040 1050
IQINDKSQDA APTLWDSLEE PHIRDTSEFE YLFSKDTTQQ KKKPLSEAYE
1060 1070 1080 1090 1100
KKNKVKKIIK LLDGKRSQTV GILISSLHLE MKDIQQAIFT VDDSVVDLET
1110 1120 1130 1140 1150
LAALYENRAQ EDELTKIRKY YETSKEEDLK LLDKPEQFLH ELAQIPNFAE
1160 1170 1180 1190 1200
RAQCIIFRAV FSEGITSLHR KVEIVTRASK GLLHMKSVKD ILALILAFGN
1210 1220 1230 1240 1250
YMNGGNRTRG QADGYSLEIL PKLKDVKSRD NGMNLVDYVV KYYLRYYDQC
1260 1270 1280 1290 1300
KHHDQEASCR GKDLFSLYFH IAVHPQRKSG LELKQEAGTD KSVFPLPEPQ
1310 1320 1330 1340 1350
DFFLASQVKF EDLLKDLRKL KRQLEASEQQ MKLVCKESPR EYLQPFKDKL
1360 1370 1380 1390 1400
EEFFKKAKKE HKMEESHLEN AQKSFETTVG YFGMKPKTGE KEVTPSYVFM
1410 1420 1430 1440 1450
VWFEFCSDFK TIWKRESKNI SKERLKMAQA SVSKLTSEKK VETKKINPTA
1460
SLKERLRQKE ASVATN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q7P6 | E9Q7P6_MOUSE | Formin-1 | Fmn1 | 1,332 | Annotation score: | ||
A0A5F8MPL0 | A0A5F8MPL0_MOUSE | Formin-1 | Fmn1 | 850 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 220 | V → A in CAA37668 (PubMed:2392150).Curated | 1 | |
Sequence conflicti | 776 | G → E in CAA37668 (PubMed:2392150).Curated | 1 | |
Sequence conflicti | 776 | G → E in CAA44244 (PubMed:1339380).Curated | 1 | |
Sequence conflicti | 866 | H → Q in CAA37668 (PubMed:2392150).Curated | 1 | |
Sequence conflicti | 866 | H → Q in CAA44244 (PubMed:1339380).Curated | 1 | |
Sequence conflicti | 950 | G → GPP in CAA37668 (PubMed:2392150).Curated | 1 | |
Sequence conflicti | 950 | G → GPP in CAA44244 (PubMed:1339380).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027214 | 1 – 682 | Missing in isoform 5. 1 PublicationAdd BLAST | 682 | |
Alternative sequenceiVSP_029424 | 1 – 623 | Missing in isoform 6. CuratedAdd BLAST | 623 | |
Alternative sequenceiVSP_029425 | 624 | P → MVTTGSPPFNTMGACYRYNP RYGQPISVPKVWKCRHRRSV TPDE in isoform 6. Curated | 1 | |
Alternative sequenceiVSP_001570 | 625 – 722 | Missing in isoform 3. CuratedAdd BLAST | 98 | |
Alternative sequenceiVSP_001571 | 626 – 627 | IA → SV in isoform 4. Curated | 2 | |
Alternative sequenceiVSP_001572 | 628 – 1466 | Missing in isoform 4. CuratedAdd BLAST | 839 | |
Alternative sequenceiVSP_027215 | 683 | N → MEEVGNSLSSRDVLEPDKSE AGLEMAQSILSKFSMKSLFG FTNKLDSLEPEEEDAVLKAF RSLEGDPAPERGDPSKGSDQ PQAEAPVPPDLKNDGKSARA ETGSEGSQGKGRSNTSSPGY ELSPATVSVDNEEVIWVRGT LVHTTSDSDSEDGDQEAEEE SSLDTQKPTTVVLCEPSQEP KDRAGDSEENTDTGNTDDTE LCAEESQRTLPETSSKLELG GDGSHPAEHSPRQDQAAEEG SQIPPAATDQTVGALASTVS KREAPEEKPFQLPAFFSGLR VLKKGATAEAGETITEIKPK DGDLALLKLTQRVQKSLGQG GPQTVKSPGRATDPKATPTL LEQLSQLLNIDMPRTEQKEA DPEFHGADEMGYSTDQESHK SPRDAHVQGGQVKARTPETA LEAFKALFIRPPKKGSTADT SELEALKRKMKHEKESLRAV FERSKSRPADSPSDPKS in isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_027216 | 1250 – 1285 | Missing in isoform 2, isoform 5 and isoform 6. 1 PublicationAdd BLAST | 36 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53599 mRNA Translation: CAA37668.1 X62379 mRNA Translation: CAA44244.1 AL672253 Genomic DNA No translation available. AL691437 Genomic DNA No translation available. AL691466 Genomic DNA No translation available. |
CCDSi | CCDS16559.1 [Q05860-1] CCDS71113.1 [Q05860-5] |
PIRi | S11515 S24407 |
RefSeqi | NP_001272388.1, NM_001285459.1 [Q05860-5] NP_034360.2, NM_010230.2 [Q05860-1] XP_011237596.1, XM_011239294.1 [Q05860-2] XP_011237597.1, XM_011239295.1 [Q05860-2] |
Genome annotation databases
Ensembli | ENSMUST00000081349; ENSMUSP00000080093; ENSMUSG00000044042 [Q05860-5] ENSMUST00000099576; ENSMUSP00000097171; ENSMUSG00000044042 [Q05860-2] ENSMUST00000102547; ENSMUSP00000099606; ENSMUSG00000044042 [Q05860-1] |
GeneIDi | 14260 |
KEGGi | mmu:14260 |
UCSCi | uc008lpk.1, mouse [Q05860-4] uc008lpl.1, mouse [Q05860-1] uc008lpn.2, mouse [Q05860-5] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53599 mRNA Translation: CAA37668.1 X62379 mRNA Translation: CAA44244.1 AL672253 Genomic DNA No translation available. AL691437 Genomic DNA No translation available. AL691466 Genomic DNA No translation available. |
CCDSi | CCDS16559.1 [Q05860-1] CCDS71113.1 [Q05860-5] |
PIRi | S11515 S24407 |
RefSeqi | NP_001272388.1, NM_001285459.1 [Q05860-5] NP_034360.2, NM_010230.2 [Q05860-1] XP_011237596.1, XM_011239294.1 [Q05860-2] XP_011237597.1, XM_011239295.1 [Q05860-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JUP | NMR | - | P | 881-888 | [»] | |
2RLY | NMR | - | P | 874-881 | [»] | |
2RM0 | NMR | - | P | 880-888 | [»] | |
SMRi | Q05860 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199711, 13 interactors |
DIPi | DIP-646N |
ELMi | Q05860 |
IntActi | Q05860, 1 interactor |
STRINGi | 10090.ENSMUSP00000099606 |
PTM databases
iPTMneti | Q05860 |
PhosphoSitePlusi | Q05860 |
Proteomic databases
MaxQBi | Q05860 |
PaxDbi | Q05860 |
PeptideAtlasi | Q05860 |
PRIDEi | Q05860 |
ProteomicsDBi | 267599 [Q05860-1] 267600 [Q05860-2] 267601 [Q05860-3] 267602 [Q05860-4] 267603 [Q05860-5] 267604 [Q05860-6] |
Protocols and materials databases
Antibodypediai | 51683, 102 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081349; ENSMUSP00000080093; ENSMUSG00000044042 [Q05860-5] ENSMUST00000099576; ENSMUSP00000097171; ENSMUSG00000044042 [Q05860-2] ENSMUST00000102547; ENSMUSP00000099606; ENSMUSG00000044042 [Q05860-1] |
GeneIDi | 14260 |
KEGGi | mmu:14260 |
UCSCi | uc008lpk.1, mouse [Q05860-4] uc008lpl.1, mouse [Q05860-1] uc008lpn.2, mouse [Q05860-5] |
Organism-specific databases
CTDi | 342184 |
MGIi | MGI:101815, Fmn1 |
Phylogenomic databases
eggNOGi | KOG1922, Eukaryota |
GeneTreei | ENSGT00940000154289 |
HOGENOMi | CLU_271027_0_0_1 |
InParanoidi | Q05860 |
OMAi | DKMLMDE |
OrthoDBi | 249504at2759 |
PhylomeDBi | Q05860 |
TreeFami | TF351317 |
Miscellaneous databases
BioGRID-ORCSi | 14260, 2 hits in 54 CRISPR screens |
ChiTaRSi | Fmn1, mouse |
EvolutionaryTracei | Q05860 |
PROi | PR:Q05860 |
RNActi | Q05860, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000044042, Expressed in substantia nigra and 219 other tissues |
ExpressionAtlasi | Q05860, baseline and differential |
Genevisiblei | Q05860, MM |
Family and domain databases
Gene3Di | 1.20.58.2220, 2 hits |
InterProi | View protein in InterPro IPR015425, FH2_Formin IPR042201, FH2_Formin_sf IPR001265, Formin_Cappuccino_subfam |
Pfami | View protein in Pfam PF02181, FH2, 1 hit |
PRINTSi | PR00828, FORMIN |
SMARTi | View protein in SMART SM00498, FH2, 1 hit |
PROSITEi | View protein in PROSITE PS51444, FH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FMN1_MOUSE | |
Accessioni | Q05860Primary (citable) accession number: Q05860 Secondary accession number(s): A2AFY9, A2AFZ0, Q05859 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | July 24, 2007 | |
Last modified: | April 7, 2021 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families