UniProtKB - Q05825 (ATPB_DROME)
ATP synthase subunit beta, mitochondrial
ATPsynbeta
Functioni
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Catalytic activityi
- EC:7.1.2.2
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 183 – 190 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- proton-transporting ATPase activity, rotational mechanism Source: UniProtKB-EC
- proton-transporting ATP synthase activity, rotational mechanism Source: UniProtKB-EC
GO - Biological processi
- ATP synthesis coupled proton transport Source: FlyBase
- mitochondrial ATP synthesis coupled proton transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | ATP synthesis, Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-1268020, Mitochondrial protein import R-DME-163210, Formation of ATP by chemiosmotic coupling R-DME-8949613, Cristae formation |
SignaLinki | Q05825 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:CG11154Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0010217, ATPsynbeta |
VEuPathDBi | VectorBase:FBgn0010217 |
Subcellular locationi
Mitochondrion
Note: Peripheral membrane protein.
Mitochondrion
- mitochondrial proton-transporting ATP synthase complex Source: GO_Central
- mitochondrial proton-transporting ATP synthase, catalytic core Source: FlyBase
- mitochondrion Source: FlyBase
Other locations
- proton-transporting ATP synthase complex, catalytic core F(1) Source: GO_Central
Keywords - Cellular componenti
CF(1), Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 31 | MitochondrionSequence analysisAdd BLAST | 31 | |
ChainiPRO_0000002448 | 32 – 505 | ATP synthase subunit beta, mitochondrialAdd BLAST | 474 |
Proteomic databases
PaxDbi | Q05825 |
PRIDEi | Q05825 |
Expressioni
Gene expression databases
Bgeei | FBgn0010217, Expressed in adult tagma (Drosophila) and 44 other tissues |
ExpressionAtlasi | Q05825, baseline and differential |
Genevisiblei | Q05825, DM |
Interactioni
Subunit structurei
F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.
Binary interactionsi
Q05825
With | #Exp. | IntAct |
---|---|---|
anon2A5 [Q9VES8] | 3 | EBI-86574,EBI-173806 |
Protein-protein interaction databases
BioGRIDi | 68655, 69 interactors |
IntActi | Q05825, 31 interactors |
MINTi | Q05825 |
STRINGi | 7227.FBpp0305828 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1350, Eukaryota |
HOGENOMi | CLU_022398_0_2_1 |
InParanoidi | Q05825 |
OMAi | FNMIMDG |
OrthoDBi | 495235at2759 |
PhylomeDBi | Q05825 |
Family and domain databases
Gene3Di | 1.10.1140.10, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01347, ATP_synth_beta_bact, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR005722, ATP_synth_F1_bsu IPR020003, ATPase_a/bsu_AS IPR004100, ATPase_F1/V1/A1_a/bsu_N IPR036121, ATPase_F1/V1/A1_a/bsu_N_sf IPR000194, ATPase_F1/V1/A1_a/bsu_nucl-bd IPR024034, ATPase_F1/V1_b/a_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00006, ATP-synt_ab, 1 hit PF02874, ATP-synt_ab_N, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF50615, SSF50615, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01039, atpD, 1 hit |
PROSITEi | View protein in PROSITE PS00152, ATPASE_ALPHA_BETA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MFALRAASKA DKNLLPFLGQ LSRSHAAKAA KAAAAANGKI VAVIGAVVDV
60 70 80 90 100
QFDDNLPPIL NALEVDNRSP RLVLEVAQHL GENTVRTIAM DGTEGLVRGQ
110 120 130 140 150
KVLDTGYPIR IPVGAETLGR IINVIGEPID ERGPIDTDKT AAIHAEAPEF
160 170 180 190 200
VQMSVEQEIL VTGIKVVDLL APYAKGGKIG LFGGAGVGKT VLIMELINNV
210 220 230 240 250
AKAHGGYSVF AGVGERTREG NDLYNEMIEG GVISLKDKTS KVALVYGQMN
260 270 280 290 300
EPPGARARVA LTGLTVAEYF RDQEGQDVLL FIDNIFRFTQ AGSEVSALLG
310 320 330 340 350
RIPSAVGYQP TLATDMGSMQ ERITTTKKGS ITSVQAIYVP ADDLTDPAPA
360 370 380 390 400
TTFAHLDATT VLSRAIAELG IYPAVDPLDS TSRIMDPNII GQEHYNVARG
410 420 430 440 450
VQKILQDYKS LQDIIAILGM DELSEEDKLT VARARKIQRF LSQPFQVAEV
460 470 480 490 500
FTGHAGKLVP LEQTIKGFSA ILAGDYDHLP EVAFYMVGPI EEVVEKADRL
AKEAA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketX2JH42 | X2JH42_DROME | ATP synthase subunit beta | ATPsynbeta ATP, ATP syn-beta, ATP-B, ATP-syn-B, ATP-syn-beta | 505 | Annotation score: | ||
L0MQ04 | L0MQ04_DROME | ATP synthase subunit beta | ATPsynbeta ATP, ATP syn-beta, ATP-B, ATP-syn-B, ATP-syn-beta | 511 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 494 – 495 | VE → CR in CAA50332 (PubMed:8373413).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71013 mRNA Translation: CAA50332.1 AE014135 Genomic DNA Translation: AAF59391.1 AY118367 mRNA Translation: AAM48396.1 |
PIRi | JN0760 |
RefSeqi | NP_001284719.1, NM_001297790.1 NP_726631.1, NM_166808.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0089186; FBpp0088250; FBgn0010217 FBtr0344754; FBpp0311086; FBgn0010217 |
GeneIDi | 43829 |
KEGGi | dme:Dmel_CG11154 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71013 mRNA Translation: CAA50332.1 AE014135 Genomic DNA Translation: AAF59391.1 AY118367 mRNA Translation: AAM48396.1 |
PIRi | JN0760 |
RefSeqi | NP_001284719.1, NM_001297790.1 NP_726631.1, NM_166808.3 |
3D structure databases
SMRi | Q05825 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68655, 69 interactors |
IntActi | Q05825, 31 interactors |
MINTi | Q05825 |
STRINGi | 7227.FBpp0305828 |
Proteomic databases
PaxDbi | Q05825 |
PRIDEi | Q05825 |
Protocols and materials databases
DNASUi | 43829 |
Genome annotation databases
EnsemblMetazoai | FBtr0089186; FBpp0088250; FBgn0010217 FBtr0344754; FBpp0311086; FBgn0010217 |
GeneIDi | 43829 |
KEGGi | dme:Dmel_CG11154 |
Organism-specific databases
CTDi | 43829 |
FlyBasei | FBgn0010217, ATPsynbeta |
VEuPathDBi | VectorBase:FBgn0010217 |
Phylogenomic databases
eggNOGi | KOG1350, Eukaryota |
HOGENOMi | CLU_022398_0_2_1 |
InParanoidi | Q05825 |
OMAi | FNMIMDG |
OrthoDBi | 495235at2759 |
PhylomeDBi | Q05825 |
Enzyme and pathway databases
Reactomei | R-DME-1268020, Mitochondrial protein import R-DME-163210, Formation of ATP by chemiosmotic coupling R-DME-8949613, Cristae formation |
SignaLinki | Q05825 |
Miscellaneous databases
BioGRID-ORCSi | 43829, 1 hit in 1 CRISPR screen |
ChiTaRSi | ATPsynbeta, fly |
GenomeRNAii | 43829 |
PROi | PR:Q05825 |
Gene expression databases
Bgeei | FBgn0010217, Expressed in adult tagma (Drosophila) and 44 other tissues |
ExpressionAtlasi | Q05825, baseline and differential |
Genevisiblei | Q05825, DM |
Family and domain databases
Gene3Di | 1.10.1140.10, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01347, ATP_synth_beta_bact, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR005722, ATP_synth_F1_bsu IPR020003, ATPase_a/bsu_AS IPR004100, ATPase_F1/V1/A1_a/bsu_N IPR036121, ATPase_F1/V1/A1_a/bsu_N_sf IPR000194, ATPase_F1/V1/A1_a/bsu_nucl-bd IPR024034, ATPase_F1/V1_b/a_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00006, ATP-synt_ab, 1 hit PF02874, ATP-synt_ab_N, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF50615, SSF50615, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01039, atpD, 1 hit |
PROSITEi | View protein in PROSITE PS00152, ATPASE_ALPHA_BETA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATPB_DROME | |
Accessioni | Q05825Primary (citable) accession number: Q05825 Secondary accession number(s): Q9V494 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | May 24, 2004 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families