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UniProtKB - Q05683 (DCE2_RAT)
Protein
Glutamate decarboxylase 2
Gene
Gad2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the production of GABA.
1 PublicationCatalytic activityi
Cofactori
pyridoxal 5'-phosphate1 Publication
Kineticsi
- KM=1.02 mM for glutamate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 558 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- glutamate binding Source: RGD
- glutamate decarboxylase activity Source: RGD
- protein-containing complex binding Source: RGD
- pyridoxal phosphate binding Source: RGD
GO - Biological processi
- chemical synaptic transmission Source: RGD
- glutamate decarboxylation to succinate Source: RGD
- neurotransmitter biosynthetic process Source: UniProtKB-KW
- response to xenobiotic stimulus Source: RGD
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Neurotransmitter biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 4.1.1.15, 5301 |
Reactomei | R-RNO-888568, GABA synthesis R-RNO-888590, GABA synthesis, release, reuptake and degradation |
SABIO-RKi | Q05683 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate decarboxylase 2Curated (EC:4.1.1.151 Publication)Alternative name(s): 65 kDa glutamic acid decarboxylase Short name: GAD-65 Glutamate decarboxylase 65 kDa isoform |
Gene namesi | Name:Gad2 Synonyms:Gad65 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2653, Gad2 |
Subcellular locationi
Plasma membrane
- presynaptic cell membrane By similarity; Lipid-anchor By similarity
Cytoplasm and Cytosol
- cytosol 1 Publication
Golgi apparatus
- Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Other locations
- Cytoplasmic vesicle By similarity
Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.By similarity
Cytosol
- cytosol Source: RGD
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- presynaptic membrane Source: UniProtKB-SubCell
Other locations
- anchored component of membrane Source: RGD
- axon Source: RGD
- cytoplasm Source: RGD
- cytoplasmic vesicle Source: RGD
- inhibitory synapse Source: MGI
- perinuclear region of cytoplasm Source: RGD
- synapse Source: RGD
- synaptic vesicle membrane Source: RGD
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 – 17 | SFGSEDGS → AFGAEDGA: No effect on glutamate decarboxylase activity. 1 Publication | 8 | |
Mutagenesisi | 30 | C → A: Abolishes palmitoylation but not membrane-association; when associates with A-45. No effect on glutamate decarboxylase activity; when associated with A-45. 2 Publications | 1 | |
Mutagenesisi | 45 | C → A: Abolishes palmitoylation but not membrane-association; when associates with A-30. No effect on glutamate decarboxylase activity; when associated with A-30. 2 Publications | 1 | |
Mutagenesisi | 73 | C → S: No effect on palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 75 | C → S: No effect on palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 80 | C → S: No effect on palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 82 | C → S: No effect on palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 101 | C → S: No effect on palmitoylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000146971 | 1 – 585 | Glutamate decarboxylase 2Add BLAST | 585 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 6 | Phosphoserine1 Publication | 1 | |
Modified residuei | 10 | Phosphoserine1 Publication | 1 | |
Modified residuei | 13 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 17 | Phosphoserine1 Publication | 1 | |
Lipidationi | 30 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 45 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 396 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked.
Phosphorylated; which does not affect kinetic parameters or subcellular location.1 Publication
Palmitoylated; which is required for presynaptic clustering.By similarity
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q05683 |
PTM databases
iPTMneti | Q05683 |
PhosphoSitePlusi | Q05683 |
SwissPalmi | Q05683 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000018200, Expressed in brain and 8 other tissues |
Genevisiblei | Q05683, RN |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 246551, 2 interactors |
ComplexPortali | CPX-3063, Glutamate decarboxylase 1/2 complex CPX-3067, Glutamate decarboxylase 2 complex |
IntActi | Q05683, 1 interactor |
STRINGi | 10116.ENSRNOP00000024901 |
Chemistry databases
BindingDBi | Q05683 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 25 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 181 – 183 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the group II decarboxylase family.Curated
Phylogenomic databases
eggNOGi | KOG0629, Eukaryota |
GeneTreei | ENSGT00940000157951 |
HOGENOMi | CLU_011856_0_0_1 |
InParanoidi | Q05683 |
OMAi | VACTCDQ |
OrthoDBi | 810772at2759 |
PhylomeDBi | Q05683 |
TreeFami | TF314688 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit |
InterProi | View protein in InterPro IPR002129, PyrdxlP-dep_de-COase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR021115, Pyridoxal-P_BS |
Pfami | View protein in Pfam PF00282, Pyridoxal_deC, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00392, DDC_GAD_HDC_YDC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q05683-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASPGSGFWS FGSEDGSGDP ENPGTARAWC QVAQKFTGGI GNKLCALLYG
60 70 80 90 100
DSEKPAESGG SVTSRAATRK VACTCDQKPC SCPKGDVNYA LLHATDLLPA
110 120 130 140 150
CEGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
160 170 180 190 200
DQPQNLEEIL THCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
210 220 230 240 250
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
260 270 280 290 300
MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
310 320 330 340 350
LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV
360 370 380 390 400
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LNGVERANSV TWNPHKMMGV
410 420 430 440 450
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
460 470 480 490 500
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYNIIKNREG YEMVFDGKPQ
510 520 530 540 550
HTNVCFWFVP PSLRVLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL
560 570 580
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 190 | G → V AA sequence (PubMed:3385490).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M72422 mRNA Translation: AAA63488.1 |
PIRi | JH0423 |
RefSeqi | NP_036695.1, NM_012563.1 |
Genome annotation databases
Ensembli | ENSRNOT00000024901; ENSRNOP00000024901; ENSRNOG00000018200 |
GeneIDi | 24380 |
KEGGi | rno:24380 |
UCSCi | RGD:2653, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M72422 mRNA Translation: AAA63488.1 |
PIRi | JH0423 |
RefSeqi | NP_036695.1, NM_012563.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3CUP | X-ray | 3.09 | B | 221-235 | [»] | |
AlphaFoldDBi | Q05683 | |||||
SMRi | Q05683 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 246551, 2 interactors |
ComplexPortali | CPX-3063, Glutamate decarboxylase 1/2 complex CPX-3067, Glutamate decarboxylase 2 complex |
IntActi | Q05683, 1 interactor |
STRINGi | 10116.ENSRNOP00000024901 |
Chemistry databases
BindingDBi | Q05683 |
PTM databases
iPTMneti | Q05683 |
PhosphoSitePlusi | Q05683 |
SwissPalmi | Q05683 |
Proteomic databases
PaxDbi | Q05683 |
Protocols and materials databases
ABCDi | Q05683, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000024901; ENSRNOP00000024901; ENSRNOG00000018200 |
GeneIDi | 24380 |
KEGGi | rno:24380 |
UCSCi | RGD:2653, rat |
Organism-specific databases
CTDi | 2572 |
RGDi | 2653, Gad2 |
Phylogenomic databases
eggNOGi | KOG0629, Eukaryota |
GeneTreei | ENSGT00940000157951 |
HOGENOMi | CLU_011856_0_0_1 |
InParanoidi | Q05683 |
OMAi | VACTCDQ |
OrthoDBi | 810772at2759 |
PhylomeDBi | Q05683 |
TreeFami | TF314688 |
Enzyme and pathway databases
BRENDAi | 4.1.1.15, 5301 |
Reactomei | R-RNO-888568, GABA synthesis R-RNO-888590, GABA synthesis, release, reuptake and degradation |
SABIO-RKi | Q05683 |
Miscellaneous databases
PROi | PR:Q05683 |
Gene expression databases
Bgeei | ENSRNOG00000018200, Expressed in brain and 8 other tissues |
Genevisiblei | Q05683, RN |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit |
InterProi | View protein in InterPro IPR002129, PyrdxlP-dep_de-COase IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR021115, Pyridoxal-P_BS |
Pfami | View protein in Pfam PF00282, Pyridoxal_deC, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00392, DDC_GAD_HDC_YDC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCE2_RAT | |
Accessioni | Q05683Primary (citable) accession number: Q05683 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1996 |
Last sequence update: | February 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families