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Protein

Exosome complex component RRP45

Gene

RRP45

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP45 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation.2 Publications

Miscellaneous

Present with 4800 molecules/cell in log phase SD medium.1 Publication

Caution

According to PubMed:17173052 and PubMed:17174896, only DIS3/RRP44 subunit of the exosome core has exonuclease activity.Curated

GO - Molecular functioni

GO - Biological processi

  • exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay Source: GO_Central
  • exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • nonfunctional rRNA decay Source: SGD
  • nuclear mRNA surveillance Source: GO_Central
  • nuclear polyadenylation-dependent mRNA catabolic process Source: GO_Central
  • nuclear polyadenylation-dependent rRNA catabolic process Source: SGD
  • nuclear polyadenylation-dependent tRNA catabolic process Source: SGD
  • nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay Source: SGD
  • nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: GO_Central
  • nuclear-transcribed mRNA catabolic process, non-stop decay Source: SGD
  • polyadenylation-dependent snoRNA 3'-end processing Source: SGD
  • U1 snRNA 3'-end processing Source: SGD
  • U4 snRNA 3'-end processing Source: SGD
  • U5 snRNA 3'-end processing Source: SGD

Keywordsi

Molecular functionRNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29845-MONOMER
ReactomeiR-SCE-429958 mRNA decay by 3' to 5' exoribonuclease
R-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Exosome complex component RRP45
Alternative name(s):
Ribosomal RNA-processing protein 45
Gene namesi
Name:RRP45
Ordered Locus Names:YDR280W
ORF Names:D9954.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR280W
SGDiS000002688 RRP45

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Exosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001399741 – 305Exosome complex component RRP45Add BLAST305

Proteomic databases

MaxQBiQ05636
PaxDbiQ05636
PRIDEiQ05636

Interactioni

Subunit structurei

Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which associates with catalytic subunits DIS3 and RRP6 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits and peripheral S1 domain-containing components CSL4, RRP4 and RRP40 located on the top of the ring structure. Interacts with LRP1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32333, 103 interactors
ComplexPortaliCPX-599 Nuclear/nucleolar exosome complex, DIS3-RRP6 variant
CPX-603 Cytoplasmic exosome complex, DIS3 variant
DIPiDIP-2084N
IntActiQ05636, 52 interactors
MINTiQ05636
STRINGi4932.YDR280W

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 20Combined sources12
Beta strandi36 – 40Combined sources5
Beta strandi46 – 51Combined sources6
Beta strandi54 – 65Combined sources12
Beta strandi69 – 71Combined sources3
Beta strandi76 – 80Combined sources5
Helixi85 – 87Combined sources3
Helixi99 – 112Combined sources14
Turni113 – 116Combined sources4
Beta strandi123 – 126Combined sources4
Turni127 – 129Combined sources3
Beta strandi130 – 142Combined sources13
Helixi147 – 161Combined sources15
Beta strandi167 – 170Combined sources4
Beta strandi173 – 176Combined sources4
Turni179 – 181Combined sources3
Beta strandi194 – 201Combined sources8
Helixi206 – 211Combined sources6
Beta strandi218 – 222Combined sources5
Helixi225 – 228Combined sources4
Beta strandi232 – 239Combined sources8
Beta strandi243 – 257Combined sources15
Helixi258 – 298Combined sources41
Beta strandi299 – 302Combined sources4

3D structure databases

ProteinModelPortaliQ05636
SMRiQ05636
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05636

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063093
HOGENOMiHOG000229504
InParanoidiQ05636
KOiK03678
OMAiMFMNSNL
OrthoDBiEOG092C46HA

Family and domain databases

CDDicd11368 RNase_PH_RRP45, 1 hit
Gene3Di3.30.230.70, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR033100 Rrp45
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 2 hits
SSF55666 SSF55666, 1 hit

Sequencei

Sequence statusi: Complete.

Q05636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDIEISAS ESKFILEALR QNYRLDGRSF DQFRDVEITF GKEFGDVSVK
60 70 80 90 100
MGNTKVHCRI SCQIAQPYED RPFEGLFVIS TEISPMAGSQ FENGNITGED
110 120 130 140 150
EVLCSRIIEK SVRRSGALDV EGLCIVAGSK CWAVRADVHF LDCDGGFIDA
160 170 180 190 200
SCIAVMAGLM HFKKPDITVH GEQIIVHPVN EREPVPLGIL HIPICVTFSF
210 220 230 240 250
FNPQDTEENI KGETNSEISI IDATLKEELL RDGVLTVTLN KNREVVQVSK
260 270 280 290 300
AGGLPMDALT LMKCCHEAYS IIEKITDQIL QLLKEDSEKR NKYAAMLTSE

NAREI
Length:305
Mass (Da):33,962
Last modified:November 1, 1996 - v1
Checksum:i65C9DB5A63678F2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51030 Genomic DNA Translation: AAB64446.1
AY557748 Genomic DNA Translation: AAS56074.1
BK006938 Genomic DNA Translation: DAA12120.1
PIRiS70136
RefSeqiNP_010566.1, NM_001180588.1

Genome annotation databases

EnsemblFungiiYDR280W; YDR280W; YDR280W
GeneIDi851874
KEGGisce:YDR280W

Similar proteinsi

Entry informationi

Entry nameiRRP45_YEAST
AccessioniPrimary (citable) accession number: Q05636
Secondary accession number(s): D6VSR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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