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Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Activity regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226Charge relay systemBy similarity1
Active sitei277Charge relay systemBy similarity1
Active sitei378Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processPlasminogen activation

Enzyme and pathway databases

ReactomeiR-BTA-6798695 Neutrophil degranulation
R-BTA-75205 Dissolution of Fibrin Clot

Protein family/group databases

MEROPSiS01.231

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:PLAU
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 28

Organism-specific databases

VGNCiVGNC:32975 PLAU

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
ChainiPRO_000002831521 – 433Urokinase-type plasminogen activatorAdd BLAST413
ChainiPRO_000002831621 – 179Urokinase-type plasminogen activator long chain ABy similarityAdd BLAST159
ChainiPRO_0000285895158 – 179Urokinase-type plasminogen activator short chain ABy similarityAdd BLAST22
ChainiPRO_0000028317181 – 433Urokinase-type plasminogen activator chain BBy similarityAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 41By similarity
Disulfide bondi35 ↔ 53By similarity
Disulfide bondi55 ↔ 64By similarity
Disulfide bondi72 ↔ 153By similarity
Disulfide bondi93 ↔ 135By similarity
Disulfide bondi124 ↔ 148By similarity
Modified residuei160PhosphoserineBy similarity1
Disulfide bondi170 ↔ 301Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi211 ↔ 227By similarity
Disulfide bondi219 ↔ 290By similarity
Disulfide bondi315 ↔ 384By similarity
Disulfide bondi347 ↔ 363By similarity
Disulfide bondi374 ↔ 402By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

PaxDbiQ05589
PRIDEiQ05589

Expressioni

Inductioni

By retinoic acid.

Gene expression databases

BgeeiENSBTAG00000005947 Expressed in 10 organ(s), highest expression level in spleen

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007806

Structurei

3D structure databases

ProteinModelPortaliQ05589
SMRiQ05589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 65EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini72 – 153KringlePROSITE-ProRule annotationAdd BLAST82
Domaini181 – 426Peptidase S1PROSITE-ProRule annotationAdd BLAST246

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 59Binds urokinase plasminogen activator surface receptorBy similarityAdd BLAST24
Regioni154 – 180Connecting peptideAdd BLAST27

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiQ05589
KOiK01348
OMAiQPWFAAI
OrthoDBiEOG091G0AH5
TreeFamiTF329901

Family and domain databases

CDDicd00108 KR, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05589-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVLLACLLV CALVVSDSDG SNEVHKESGE SNCGCLNGGK CVTYKYFSNI
60 70 80 90 100
QRCSCPKKFQ GEHCEIDTSK TCYQGNGHSY RGKANRDLSG RPCLAWDSPT
110 120 130 140 150
VLLKMYHAHR SDAIQLGLGK HNYCRNPDNQ RRPWCYVQIG LKQFVQFCMV
160 170 180 190 200
QDCSVGKSPS SPREKEEFQC GQKALRPRFK IVGGQVTNAE NQPWFAAIYR
210 220 230 240 250
RHRGGSITYL CGGSLISPCW VVSATHCFID HPKKENYIVY LGQSRLNSDT
260 270 280 290 300
RGEMQFEVEK LILHEDYSAE SLAHHNDIAL LKIRTSRGQC AQPSRSIQTI
310 320 330 340 350
CLPPEHEDAH SRTRCEITGF GKENPSDYRY SDELKMTFVS LVSHEVCQQP
360 370 380 390 400
HYYGAEVTDK MLCAADPQWE TDSCQGDSGG PLVCTIQGRL TLTGIVSWGR
410 420 430
DCAMKYKPGV YTRVSKFLPW INTHTRGEIN LVL
Length:433
Mass (Da):48,730
Last modified:February 1, 1994 - v1
Checksum:i4DE1B8D4DA47027A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189A → T in CAA59796 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03546 mRNA Translation: AAA51419.1
BT030737 mRNA Translation: ABS45053.1
BC122657 mRNA Translation: AAI22658.1
X85801 mRNA Translation: CAA59796.1
PIRiJN0560
RefSeqiNP_776572.1, NM_174147.2
XP_005226381.1, XM_005226324.1
XP_015316474.1, XM_015460988.1
UniGeneiBt.2519

Genome annotation databases

EnsembliENSBTAT00000007806; ENSBTAP00000007806; ENSBTAG00000005947
GeneIDi281408
KEGGibta:281408

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03546 mRNA Translation: AAA51419.1
BT030737 mRNA Translation: ABS45053.1
BC122657 mRNA Translation: AAI22658.1
X85801 mRNA Translation: CAA59796.1
PIRiJN0560
RefSeqiNP_776572.1, NM_174147.2
XP_005226381.1, XM_005226324.1
XP_015316474.1, XM_015460988.1
UniGeneiBt.2519

3D structure databases

ProteinModelPortaliQ05589
SMRiQ05589
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007806

Protein family/group databases

MEROPSiS01.231

Proteomic databases

PaxDbiQ05589
PRIDEiQ05589

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007806; ENSBTAP00000007806; ENSBTAG00000005947
GeneIDi281408
KEGGibta:281408

Organism-specific databases

CTDi5328
VGNCiVGNC:32975 PLAU

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000119133
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiQ05589
KOiK01348
OMAiQPWFAAI
OrthoDBiEOG091G0AH5
TreeFamiTF329901

Enzyme and pathway databases

ReactomeiR-BTA-6798695 Neutrophil degranulation
R-BTA-75205 Dissolution of Fibrin Clot

Gene expression databases

BgeeiENSBTAG00000005947 Expressed in 10 organ(s), highest expression level in spleen

Family and domain databases

CDDicd00108 KR, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
IPR034814 Urokinase
PANTHERiPTHR24264:SF38 PTHR24264:SF38, 1 hit
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUROK_BOVIN
AccessioniPrimary (citable) accession number: Q05589
Secondary accession number(s): A7E3W6, Q0IIG3, Q28209
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 12, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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