PRKCZ

Functionⁱ

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro (PubMed:17313651).7 Publications

Isoform 2: Involved in late synaptic long term potention phasae in CA1 hippocampal cells and long term memory maintenance.By similarity

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.3 Publications

Activity regulationⁱ

Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to the interaction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-410 (activation loop of the kinase domain) and Thr-560 (turn motif), need to be phosphorylated for its full activation. Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator (By similarity). Isoform 2: Constitutively active (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	281	ATPPROSITE-ProRule annotation		1
Active siteⁱ	376	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	130 – 180	Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST		51
Nucleotide bindingⁱ	258 – 266	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

14-3-3 protein binding Source: Ensembl
ATP binding Source: UniProtKB-KW
insulin receptor substrate binding
Source: BHF-UCL
Inferred by curatorⁱ
- 15069075
metal ion binding Source: UniProtKB-KW
phospholipase binding Source: Ensembl
potassium channel regulator activity Source: Ensembl
protein domain specific binding Source: Ensembl
protein kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 10770950
protein kinase binding Source: Ensembl
protein kinase C activity Source: Reactome
protein serine/threonine kinase activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 15069075

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of phospholipase D activity Source: Ensembl
activation of protein kinase B activity Source: Ensembl
cell migration Source: Ensembl
cellular response to insulin stimulus Source: Ensembl
establishment of cell polarity Source: UniProtKB
inflammatory response Source: UniProtKB-KW
intracellular signal transduction
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
long-term memory Source: Ensembl
long-term synaptic potentiation Source: UniProtKB
membrane depolarization Source: Ensembl
membrane hyperpolarization Source: Ensembl
microtubule cytoskeleton organization Source: Ensembl
negative regulation of apoptotic process
Source: ProtInc
Traceable author statementⁱ
- 10770953
negative regulation of hydrolase activity Source: Ensembl
negative regulation of insulin receptor signaling pathway
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 15069075
negative regulation of peptidyl-tyrosine phosphorylation
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 15069075
negative regulation of protein complex assembly
Source: BHF-UCL
Inferred from mutant phenotypeⁱ
- 15069075
neuron projection extension Source: Ensembl
peptidyl-serine phosphorylation
Source: BHF-UCL
Inferred from direct assayⁱ
- 15069075
positive regulation of cell-matrix adhesion Source: Ensembl
positive regulation of cell proliferation Source: Ensembl
positive regulation of ERK1 and ERK2 cascade
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11035106
positive regulation of excitatory postsynaptic potential Source: UniProtKB
positive regulation of insulin receptor signaling pathway Source: UniProtKB
positive regulation of interleukin-10 secretion Source: UniProtKB
positive regulation of interleukin-13 secretion Source: UniProtKB
positive regulation of interleukin-4 production Source: UniProtKB
positive regulation of interleukin-5 secretion Source: UniProtKB
positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
positive regulation of T-helper 2 cell cytokine production Source: UniProtKB
positive regulation of T-helper 2 cell differentiation Source: UniProtKB
protein heterooligomerization Source: Ensembl
protein kinase C signaling Source: Ensembl
protein localization to plasma membrane Source: Ensembl
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 10770950
- 17313651
regulation of neurotransmitter receptor localization to postsynaptic specialization membrane Source: Ensembl
signal transduction
Source: ProtInc
Traceable author statementⁱ
- 8224878
transforming growth factor beta receptor signaling pathway Source: Reactome
vesicle transport along microtubule Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Inflammatory response
Ligand	ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAⁱ	2.7.11.13 2681
Reactomeⁱ	R-HSA-114604 GPVI-mediated activation cascade R-HSA-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-HSA-5218921 VEGFR2 mediated cell proliferation
SignaLinkⁱ	Q05513
SIGNORⁱ	Q05513

Protein family/group databases

MoonDBⁱ	Q05513 Predicted

MoonDBⁱ

Q05513 Predicted

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein kinase C zeta type (EC:2.7.11.133 Publications) Alternative name(s): nPKC-zeta
Gene namesⁱ	Name:PRKCZ Synonyms:PKC2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000067606.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:9412 PRKCZ
MIMⁱ	176982 gene
neXtProtⁱ	NX_Q05513

Keywords - Cellular componentⁱ

Cell junction, Cytoplasm, Endosome, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	19	K → A: No effect on interaction with SQSTM1 and PARD6B. 1 Publication	1
Mutagenesisⁱ	62	D → A: Loss of interaction with SQSTM1 and PARD6B. 1 Publication	1
Mutagenesisⁱ	66	D → A: Loss of interaction with SQSTM1 and PARD6B. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	5590
Open Targets More...OpenTargetsⁱ	ENSG00000067606
PharmGKBⁱ	PA33775

Chemistry databases

ChEMBLⁱ	CHEMBL3438
Drug and drug target database More...DrugBankⁱ	DB00675 Tamoxifen
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	1491

Polymorphism and mutation databases

BioMutaⁱ	PRKCZ
DMDMⁱ	68067736

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000055701	1 – 592	Protein kinase C zeta typeAdd BLAST		592

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	410	Phosphothreonine; by PDPK1 and PI3K3 Publications	1
Modified residueⁱ	560	PhosphothreonineCombined sources	1
Modified residueⁱ	591	PhosphoserineBy similarity	1

Post-translational modificationⁱ

CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.4 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q05513
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q05513
PaxDbⁱ	Q05513
PeptideAtlas More...PeptideAtlasⁱ	Q05513
PRIDEⁱ	Q05513
ProteomicsDBⁱ	58330 58331 [Q05513-2]

PTM databases

iPTMnetⁱ	Q05513
PhosphoSitePlusⁱ	Q05513

Miscellaneous databases

PMAP-CutDBⁱ	Q05513

PMAP-CutDBⁱ

Q05513

Expressionⁱ

Tissue specificityⁱ

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Gene expression databases

Bgeeⁱ	ENSG00000067606 Expressed in 191 organ(s), highest expression level in chorionic villus
CleanExⁱ	HS_PRKCZ
ExpressionAtlasⁱ	Q05513 baseline and differential
Genevisibleⁱ	Q05513 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA021851

HPAⁱ

HPA021851

Interactionⁱ

Subunit structureⁱ

Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminal region). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529). Interacts with VAMP2 (PubMed:17313651). Forms a complex with WDFY2 and VAMP2 (PubMed:17313651).By similarityCurated12 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
AGER	Q15109	6	EBI-295351,EBI-1646426
AKT1	P31749	2	EBI-295351,EBI-296087
BCL10	O95999	3	EBI-295351,EBI-958922
C1QBP	Q07021	2	EBI-295351,EBI-347528
F11R	Q9Y624	2	EBI-295351,EBI-742600
HSP90AB1	P08238	2	EBI-295351,EBI-352572
NCF1	P14598	3	EBI-295351,EBI-395044
NUMB	P49757-1	4	EBI-295351,EBI-7199607
numb	P16554	2	EBI-295351,EBI-429581	From Drosophila melanogaster.
PARD6A	Q9NPB6	8	EBI-295351,EBI-81876
PARD6B	Q9BYG5	9	EBI-295351,EBI-295391
PARD6G	Q9BYG4	3	EBI-295351,EBI-295417
PRKCI	P41743	4	EBI-295351,EBI-286199
PRKCQ	Q04759	3	EBI-295351,EBI-374762
VHL	P40337	3	EBI-295351,EBI-301246

GO - Molecular functionⁱ

14-3-3 protein binding Source: Ensembl
insulin receptor substrate binding
Source: BHF-UCL
Inferred by curatorⁱ
- 15069075
phospholipase binding Source: Ensembl
protein domain specific binding Source: Ensembl
protein kinase binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	111576, 87 interactors
CORUMⁱ	Q05513
ELMⁱ	Q05513
IntActⁱ	Q05513, 37 interactors
Molecular INTeraction database More...MINTⁱ	Q05513
STRINGⁱ	9606.ENSP00000367830

Chemistry databases

BindingDBⁱ

Q05513

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q05513
SMRⁱ	Q05513
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	15 – 98	PB1PROSITE-ProRule annotationAdd BLAST	84
Domainⁱ	252 – 518	Protein kinasePROSITE-ProRule annotationAdd BLAST	267
Domainⁱ	519 – 590	AGC-kinase C-terminalAdd BLAST	72

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	79 – 145	Interaction with SQSTM1By similarityAdd BLAST		67

Domainⁱ

The PB1 domain mediate mutually exclusive interactions with SQSTM1 and PARD6B.1 Publication

The C1 domain does not bind the diacylglycerol (DAG).1 Publication

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	130 – 180	Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST		51

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0695 Eukaryota ENOG410ZMG2 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150815
HOGENOMⁱ	HOG000233033
HOVERGENⁱ	HBG108317
InParanoidⁱ	Q05513
KEGG Orthology (KO) More...KOⁱ	K18952
OMAⁱ	CQRRDEG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03Q9
PhylomeDBⁱ	Q05513
TreeFamⁱ	TF102004

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00029 C1, 1 hit cd06404 PB1_aPKC, 1 hit cd05617 STKc_aPKC_zeta, 1 hit
InterProⁱ	View protein in InterPro IPR000961 AGC-kinase_C IPR034662 aPKC_zeta IPR020454 DAG/PE-bd IPR011009 Kinase-like_dom_sf IPR034877 PB1_aPKC IPR000270 PB1_dom IPR002219 PE/DAG-bd IPR012233 PKC IPR017892 Pkinase_C IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00130 C1_1, 1 hit PF00564 PB1, 1 hit PF00069 Pkinase, 1 hit PF00433 Pkinase_C, 1 hit
PIRSFⁱ	PIRSF000554 PKC_zeta, 1 hit
PRINTSⁱ	PR00008 DAGPEDOMAIN
SMARTⁱ	View protein in SMART SM00109 C1, 1 hit SM00666 PB1, 1 hit SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS51745 PB1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS00479 ZF_DAG_PE_1, 1 hit PS50081 ZF_DAG_PE_2, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative promoter usage and alternative splicing. Align Add to basket

This entry has 3 described isoforms and 15 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q05513-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MPSRTGPKME GSGGRVRLKA HYGGDIFITS VDAATTFEEL CEEVRDMCRL 
        60         70         80         90        100
HQQHPLTLKW VDSEGDPCTV SSQMELEEAF RLARQCRDEG LIIHVFPSTP 
       110        120        130        140        150
EQPGLPCPGE DKSIYRRGAR RWRKLYRANG HLFQAKRFNR RAYCGQCSER 
       160        170        180        190        200
IWGLARQGYR CINCKLLVHK RCHGLVPLTC RKHMDSVMPS QEPPVDDKNE 
       210        220        230        240        250
DADLPSEETD GIAYISSSRK HDSIKDDSED LKPVIDGMDG IKISQGLGLQ 
       260        270        280        290        300
DFDLIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE 
       310        320        330        340        350
KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE 
       360        370        380        390        400
EHARFYAAEI CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE 
       410        420        430        440        450
GLGPGDTTST FCGTPNYIAP EILRGEEYGF SVDWWALGVL MFEMMAGRSP 
       460        470        480        490        500
FDIITDNPDM NTEDYLFQVI LEKPIRIPRF LSVKASHVLK GFLNKDPKER 
       510        520        530        540        550
LGCRPQTGFS DIKSHAFFRS IDWDLLEKKQ ALPPFQPQIT DDYGLDNFDT 
       560        570        580        590 
QFTSEPVQLT PDDEDAIKRI DQSEFEGFEY INPLLLSTEE SV

Length:592

Mass (Da):67,660

Last modified:June 21, 2005 - v4

Checksum:ⁱ88CCD0577E6A596C

GO

Isoform 2 (identifier: Q05513-2) [UniParc]FASTA Add to basket

Also known as: PKMzetaBy similarity

The sequence of this isoform differs from the canonical sequence as follows:
1-183: Missing.

« Hide

        10         20         30         40         50
MDSVMPSQEP PVDDKNEDAD LPSEETDGIA YISSSRKHDS IKDDSEDLKP 
        60         70         80         90        100
VIDGMDGIKI SQGLGLQDFD LIRVIGRGSY AKVLLVRLKK NDQIYAMKVV 
       110        120        130        140        150
KKELVHDDED IDWVQTEKHV FEQASSNPFL VGLHSCFQTT SRLFLVIEYV 
       160        170        180        190        200
NGGDLMFHMQ RQRKLPEEHA RFYAAEICIA LNFLHERGII YRDLKLDNVL 
       210        220        230        240        250
LDADGHIKLT DYGMCKEGLG PGDTTSTFCG TPNYIAPEIL RGEEYGFSVD 
       260        270        280        290        300
WWALGVLMFE MMAGRSPFDI ITDNPDMNTE DYLFQVILEK PIRIPRFLSV 
       310        320        330        340        350
KASHVLKGFL NKDPKERLGC RPQTGFSDIK SHAFFRSIDW DLLEKKQALP 
       360        370        380        390        400
PFQPQITDDY GLDNFDTQFT SEPVQLTPDD EDAIKRIDQS EFEGFEYINP 

LLLSTEESV

Note: Produced by alternative promoter usage.By similarity

Show »

Length:409

Mass (Da):46,622

Checksum:ⁱ21C316A4A2B418BD

GO

Isoform 3 (identifier: Q05513-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-112: MPSRTGPKME...PGLPCPGEDK → MLTPRTDE

« Hide

        10         20         30         40         50
MLTPRTDESI YRRGARRWRK LYRANGHLFQ AKRFNRRAYC GQCSERIWGL 
        60         70         80         90        100
ARQGYRCINC KLLVHKRCHG LVPLTCRKHM DSVMPSQEPP VDDKNEDADL 
       110        120        130        140        150
PSEETDGIAY ISSSRKHDSI KDDSEDLKPV IDGMDGIKIS QGLGLQDFDL 
       160        170        180        190        200
IRVIGRGSYA KVLLVRLKKN DQIYAMKVVK KELVHDDEDI DWVQTEKHVF 
       210        220        230        240        250
EQASSNPFLV GLHSCFQTTS RLFLVIEYVN GGDLMFHMQR QRKLPEEHAR 
       260        270        280        290        300
FYAAEICIAL NFLHERGIIY RDLKLDNVLL DADGHIKLTD YGMCKEGLGP 
       310        320        330        340        350
GDTTSTFCGT PNYIAPEILR GEEYGFSVDW WALGVLMFEM MAGRSPFDII 
       360        370        380        390        400
TDNPDMNTED YLFQVILEKP IRIPRFLSVK ASHVLKGFLN KDPKERLGCR 
       410        420        430        440        450
PQTGFSDIKS HAFFRSIDWD LLEKKQALPP FQPQITDDYG LDNFDTQFTS 
       460        470        480 
EPVQLTPDDE DAIKRIDQSE FEGFEYINPL LLSTEESV

Note: No experimental confirmation available.

Show »

Length:488

Mass (Da):56,136

Checksum:ⁱ219A7C60FCEF0905

GO

Computationally mapped potential isoform sequencesⁱ

There are 15 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name	Protein names	Gene names	Length	Annotation

	E9PBE1	E9PBE1_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	347	Annotation score:
	F2Z3C5	F2Z3C5_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	181	Annotation score:
	D6RAU1	D6RAU1_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	136	Annotation score:
	F2Z2H9	F2Z2H9_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	105	Annotation score:
	D6RGG1	D6RGG1_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	109	Annotation score:
	D6RBD4	D6RBD4_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	98	Annotation score:
	D6REZ8	D6REZ8_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	65	Annotation score:
	D6RG01	D6RG01_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	82	Annotation score:
	J3KRP7	J3KRP7_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	61	Annotation score:
	D6RDM0	D6RDM0_HUMAN	Protein kinase C zeta type Protein kinase C zeta type	PRKCZ	73	Annotation score:
There are more potential isoformsShow all

Sequence cautionⁱ

The sequence CAA78813 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	6	G → D in AAA36488 (PubMed:8224878).Curated	1
Sequence conflictⁱ	198	K → R in BAF83684 (PubMed:14702039).Curated	1
Sequence conflictⁱ	262	S → T in AAA36488 (PubMed:8224878).Curated	1
Sequence conflictⁱ	281	K → R in BAH11883 (PubMed:14702039).Curated	1
Sequence conflictⁱ	366	F → L in BAF83684 (PubMed:14702039).Curated	1
Sequence conflictⁱ	500	R → G in BAF83684 (PubMed:14702039).Curated	1
Sequence conflictⁱ	522	D → G in BAF83684 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_050560	49	R → H. Corresponds to variant dbSNP:rs35271800Ensembl.	1
Natural variantⁱVAR_042310	84	R → H1 PublicationCorresponds to variant dbSNP:rs56017162Ensembl.	1
Natural variantⁱVAR_035467	514	S → F in a colorectal cancer sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_042311	519	R → C in a colorectal adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs376894109Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_041904	1 – 183	Missing in isoform 2. 1 PublicationAdd BLAST		183
Alternative sequenceⁱVSP_046347	1 – 112	MPSRT…PGEDK → MLTPRTDE in isoform 3. 1 PublicationAdd BLAST		112

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L14283 mRNA Translation: AAA36488.1 BT007082 mRNA Translation: AAP35745.1 AK290995 mRNA Translation: BAF83684.1 AK294649 mRNA Translation: BAH11833.1 AK294782 mRNA Translation: BAH11883.1 AL162271 Genomic DNA No translation available. AL391845 Genomic DNA No translation available. AL590822 Genomic DNA No translation available. AL645703 Genomic DNA No translation available. BC008058 mRNA Translation: AAH08058.1 BC014270 mRNA Translation: AAH14270.1 Z15108 mRNA Translation: CAA78813.1 Different initiation.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37.1 [Q05513-1] CCDS41229.1 [Q05513-2] CCDS55563.1 [Q05513-3]
PIRⁱ	JN0877
NCBI Reference Sequences More...RefSeqⁱ	NP_001028753.1, NM_001033581.1 [Q05513-2] NP_001028754.1, NM_001033582.1 [Q05513-2] NP_001229803.1, NM_001242874.1 [Q05513-3] NP_002735.3, NM_002744.4 [Q05513-1] XP_016857288.1, XM_017001799.1
UniGeneⁱ	Hs.496255

Genome annotation databases

Ensemblⁱ	ENST00000378567; ENSP00000367830; ENSG00000067606 [Q05513-1] ENST00000400921; ENSP00000383712; ENSG00000067606 [Q05513-2] ENST00000461106; ENSP00000426412; ENSG00000067606 [Q05513-3]
GeneIDⁱ	5590
KEGGⁱ	hsa:5590
UCSC genome browser More...UCSCⁱ	uc001aiq.3 human [Q05513-1]

Keywords - Coding sequence diversityⁱ

Alternative promoter usage, Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q05513	Protein kinase C		PONAB		592	UniRef100_Q05513
Protein kinase C		GORGO		592
Non-specific serine/threonine protein kinase		PANTR		409
Non-specific serine/threonine protein kinase		MACNE		409
Isoform 2 of Protein kinase C zeta type		HUMAN		409
+4
Q05513-3	Protein kinase C		PONAB		488	UniRef100_A0A2J8URG9
Protein kinase C		CHLSB		488
Protein kinase C		GORGO		488
Protein kinase C		MACNE		488

Protein	Similar proteins		Species	Score	Length	Source
Q05513	Protein kinase C zeta type	)	RAT		592	UniRef90_P09217
Protein kinase C zeta type		MOUSE		592
Protein kinase C zeta type		RABIT		591
protein kinase C zeta type		URSMA		650
Protein kinase C		Cebus capucinus imitator		605
+187
Q05513-3	Protein kinase C		FUKDA		609	UniRef90_A0A091DNE0
protein kinase C zeta type-like		DIPOR		96
Protein kinase C		PONAB		488
Protein kinase C		CHLSB		488
Protein kinase C		GORGO		488
+18

Protein	Similar proteins		Species	Score	Length	Source
Q05513	Protein kinase C zeta type	)	RAT		592	UniRef50_P09217
Protein kinase C zeta type		MOUSE		592
Protein kinase C zeta type		RABIT		591
Protein kinase C-like 3		CAEEL		597
Protein kinase C-like 3		CAEBR		597
+834
Q05513-3	Atypical protein kinase C		DROME		606	UniRef50_A1Z9X0
Protein kinase C		DROPS		609
Protein kinase C		DROER		608
Protein kinase C		DROSI		608
Protein kinase C		DROGR		607
+321

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L14283 mRNA Translation: AAA36488.1 BT007082 mRNA Translation: AAP35745.1 AK290995 mRNA Translation: BAF83684.1 AK294649 mRNA Translation: BAH11833.1 AK294782 mRNA Translation: BAH11883.1 AL162271 Genomic DNA No translation available. AL391845 Genomic DNA No translation available. AL590822 Genomic DNA No translation available. AL645703 Genomic DNA No translation available. BC008058 mRNA Translation: AAH08058.1 BC014270 mRNA Translation: AAH14270.1 Z15108 mRNA Translation: CAA78813.1 Different initiation.
CCDSⁱ	CCDS37.1 [Q05513-1] CCDS41229.1 [Q05513-2] CCDS55563.1 [Q05513-3]
PIRⁱ	JN0877
RefSeqⁱ	NP_001028753.1, NM_001033581.1 [Q05513-2] NP_001028754.1, NM_001033582.1 [Q05513-2] NP_001229803.1, NM_001242874.1 [Q05513-3] NP_002735.3, NM_002744.4 [Q05513-1] XP_016857288.1, XM_017001799.1
UniGeneⁱ	Hs.496255

3D structure databases

ProteinModelPortalⁱ	Q05513
SMRⁱ	Q05513
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	111576, 87 interactors
CORUMⁱ	Q05513
ELMⁱ	Q05513
IntActⁱ	Q05513, 37 interactors
MINTⁱ	Q05513
STRINGⁱ	9606.ENSP00000367830

Chemistry databases

BindingDBⁱ	Q05513
ChEMBLⁱ	CHEMBL3438
DrugBankⁱ	DB00675 Tamoxifen
GuidetoPHARMACOLOGYⁱ	1491

Protein family/group databases

MoonDBⁱ	Q05513 Predicted

MoonDBⁱ

Q05513 Predicted

PTM databases

iPTMnetⁱ	Q05513
PhosphoSitePlusⁱ	Q05513

Polymorphism and mutation databases

BioMutaⁱ	PRKCZ
DMDMⁱ	68067736

Proteomic databases

EPDⁱ	Q05513
MaxQBⁱ	Q05513
PaxDbⁱ	Q05513
PeptideAtlasⁱ	Q05513
PRIDEⁱ	Q05513
ProteomicsDBⁱ	58330 58331 [Q05513-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	5590
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000378567; ENSP00000367830; ENSG00000067606 [Q05513-1] ENST00000400921; ENSP00000383712; ENSG00000067606 [Q05513-2] ENST00000461106; ENSP00000426412; ENSG00000067606 [Q05513-3]
GeneIDⁱ	5590
KEGGⁱ	hsa:5590
UCSCⁱ	uc001aiq.3 human [Q05513-1]

Organism-specific databases

CTDⁱ	5590
DisGeNETⁱ	5590
EuPathDBⁱ	HostDB:ENSG00000067606.15
GeneCardsⁱ	PRKCZ
H-InvDBⁱ	HIX0000049 HIX0029201
HGNCⁱ	HGNC:9412 PRKCZ
HPAⁱ	HPA021851
MIMⁱ	176982 gene
neXtProtⁱ	NX_Q05513
OpenTargetsⁱ	ENSG00000067606
PharmGKBⁱ	PA33775
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0695 Eukaryota ENOG410ZMG2 LUCA
GeneTreeⁱ	ENSGT00930000150815
HOGENOMⁱ	HOG000233033
HOVERGENⁱ	HBG108317
InParanoidⁱ	Q05513
KOⁱ	K18952
OMAⁱ	CQRRDEG
OrthoDBⁱ	EOG091G03Q9
PhylomeDBⁱ	Q05513
TreeFamⁱ	TF102004

Enzyme and pathway databases

BRENDAⁱ	2.7.11.13 2681
Reactomeⁱ	R-HSA-114604 GPVI-mediated activation cascade R-HSA-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-HSA-5218921 VEGFR2 mediated cell proliferation
SignaLinkⁱ	Q05513
SIGNORⁱ	Q05513

Miscellaneous databases

ChiTaRSⁱ	PRKCZ human
GeneWikiⁱ	Protein_kinase_M_zeta/Protein_kinase_C_zeta
GenomeRNAiⁱ	5590
PMAP-CutDBⁱ	Q05513
Protein Ontology More...PROⁱ	PR:Q05513
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000067606 Expressed in 191 organ(s), highest expression level in chorionic villus
CleanExⁱ	HS_PRKCZ
ExpressionAtlasⁱ	Q05513 baseline and differential
Genevisibleⁱ	Q05513 HS

Family and domain databases

CDDⁱ	cd00029 C1, 1 hit cd06404 PB1_aPKC, 1 hit cd05617 STKc_aPKC_zeta, 1 hit
InterProⁱ	View protein in InterPro IPR000961 AGC-kinase_C IPR034662 aPKC_zeta IPR020454 DAG/PE-bd IPR011009 Kinase-like_dom_sf IPR034877 PB1_aPKC IPR000270 PB1_dom IPR002219 PE/DAG-bd IPR012233 PKC IPR017892 Pkinase_C IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfamⁱ	View protein in Pfam PF00130 C1_1, 1 hit PF00564 PB1, 1 hit PF00069 Pkinase, 1 hit PF00433 Pkinase_C, 1 hit
PIRSFⁱ	PIRSF000554 PKC_zeta, 1 hit
PRINTSⁱ	PR00008 DAGPEDOMAIN
SMARTⁱ	View protein in SMART SM00109 C1, 1 hit SM00666 PB1, 1 hit SM00133 S_TK_X, 1 hit SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS51285 AGC_KINASE_CTER, 1 hit PS51745 PB1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS00479 ZF_DAG_PE_1, 1 hit PS50081 ZF_DAG_PE_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KPCZ_HUMAN
Accessionⁱ	Q05513Primary (citable) accession number: Q05513 Secondary accession number(s): A8K4N0 Q969S4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
	Last sequence update:	June 21, 2005
	Last modified:	November 7, 2018
	This is version 213 of the entry and version 4 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

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UniProtKB - Q05513 (KPCZ_HUMAN)

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Protein kinase C zeta type

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

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Keywords - Cellular componenti

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

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Amino acid modifications

Keywords - PTMi

Proteomic databases

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Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

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<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

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Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ