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Protein

Glutamate decarboxylase 2

Gene

GAD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=2.15 mM for glutamate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei558Substrate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • chemical synaptic transmission Source: ProtInc
    • glutamate decarboxylation to succinate Source: ProtInc
    • neurotransmitter biosynthetic process Source: UniProtKB-KW
    • response to drug Source: Ensembl

    Keywordsi

    Molecular functionDecarboxylase, Lyase
    Biological processNeurotransmitter biosynthesis
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:HS06208-MONOMER
    BRENDAi4.1.1.15 2681
    ReactomeiR-HSA-888568 GABA synthesis
    R-HSA-888590 GABA synthesis, release, reuptake and degradation
    SABIO-RKiQ05329

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase 2 (EC:4.1.1.15)
    Alternative name(s):
    65 kDa glutamic acid decarboxylase
    Short name:
    GAD-65
    Glutamate decarboxylase 65 kDa isoform
    Gene namesi
    Name:GAD2
    Synonyms:GAD65
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 10

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000136750.11
    HGNCiHGNC:4093 GAD2
    MIMi138275 gene
    neXtProtiNX_Q05329

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi2572
    OpenTargetsiENSG00000136750
    PharmGKBiPA28508

    Chemistry databases

    ChEMBLiCHEMBL2952
    DrugBankiDB00142 L-Glutamic Acid

    Polymorphism and mutation databases

    BioMutaiGAD2
    DMDMi1352216

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001469681 – 585Glutamate decarboxylase 2Add BLAST585

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei3Phosphoserine1 Publication1
    Modified residuei6Phosphoserine1 Publication1
    Modified residuei10Phosphoserine1 Publication1
    Modified residuei13Phosphoserine1 Publication1
    Lipidationi30S-palmitoyl cysteine1 Publication1
    Lipidationi45S-palmitoyl cysteine1 Publication1
    Modified residuei396N6-(pyridoxal phosphate)lysine1

    Post-translational modificationi

    Phosphorylated; which does not affect kinetic parameters or subcellular location.1 Publication
    Palmitoylated; which is required for presynaptic clustering.1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ05329
    PeptideAtlasiQ05329
    PRIDEiQ05329
    ProteomicsDBi58319

    PTM databases

    iPTMnetiQ05329
    PhosphoSitePlusiQ05329
    SwissPalmiQ05329

    Expressioni

    Gene expression databases

    BgeeiENSG00000136750
    CleanExiHS_GAD2
    ExpressionAtlasiQ05329 baseline and differential
    GenevisibleiQ05329 HS

    Organism-specific databases

    HPAiCAB078177
    HPA044637

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Binary interactionsi

    Show more details

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi108846, 8 interactors
    ComplexPortaliCPX-3033 Glutamate decarboxylase 2 complex
    CPX-3065 Glutamate decarboxylase 1/2 complex
    DIPiDIP-29293N
    IntActiQ05329, 39 interactors
    STRINGi9606.ENSP00000259271

    Structurei

    Secondary structure

    1585
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi89 – 91Combined sources3
    Helixi94 – 96Combined sources3
    Helixi104 – 126Combined sources23
    Helixi138 – 144Combined sources7
    Helixi156 – 169Combined sources14
    Beta strandi178 – 183Combined sources6
    Helixi188 – 200Combined sources13
    Turni207 – 209Combined sources3
    Helixi211 – 228Combined sources18
    Helixi231 – 233Combined sources3
    Beta strandi235 – 242Combined sources8
    Helixi243 – 258Combined sources16
    Helixi262 – 265Combined sources4
    Helixi267 – 269Combined sources3
    Beta strandi273 – 278Combined sources6
    Helixi284 – 291Combined sources8
    Helixi296 – 298Combined sources3
    Beta strandi299 – 302Combined sources4
    Helixi312 – 324Combined sources13
    Beta strandi328 – 337Combined sources10
    Turni339 – 341Combined sources3
    Helixi347 – 357Combined sources11
    Beta strandi360 – 365Combined sources6
    Helixi368 – 373Combined sources6
    Turni375 – 377Combined sources3
    Helixi378 – 381Combined sources4
    Helixi384 – 386Combined sources3
    Beta strandi388 – 392Combined sources5
    Beta strandi405 – 411Combined sources7
    Helixi414 – 419Combined sources6
    Helixi435 – 437Combined sources3
    Helixi440 – 442Combined sources3
    Helixi452 – 486Combined sources35
    Beta strandi491 – 497Combined sources7
    Beta strandi500 – 502Combined sources3
    Beta strandi504 – 508Combined sources5
    Turni511 – 515Combined sources5
    Helixi523 – 526Combined sources4
    Helixi529 – 540Combined sources12
    Beta strandi544 – 550Combined sources7
    Beta strandi553 – 559Combined sources7
    Helixi568 – 581Combined sources14

    3D structure databases

    ProteinModelPortaliQ05329
    SMRiQ05329
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05329

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni181 – 183Substrate binding3

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiKOG0629 Eukaryota
    COG0076 LUCA
    GeneTreeiENSGT00760000119205
    HOGENOMiHOG000005382
    HOVERGENiHBG004980
    InParanoidiQ05329
    KOiK01580
    OMAiWRAKGTT
    OrthoDBiEOG091G07ZU
    PhylomeDBiQ05329
    TreeFamiTF314688

    Family and domain databases

    Gene3Di3.40.640.10, 1 hit
    InterProiView protein in InterPro
    IPR002129 PyrdxlP-dep_de-COase
    IPR015424 PyrdxlP-dep_Trfase
    IPR015421 PyrdxlP-dep_Trfase_major
    IPR021115 Pyridoxal-P_BS
    PfamiView protein in Pfam
    PF00282 Pyridoxal_deC, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit
    PROSITEiView protein in PROSITE
    PS00392 DDC_GAD_HDC_YDC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q05329-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG
    60 70 80 90 100
    DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA
    110 120 130 140 150
    CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
    160 170 180 190 200
    DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
    210 220 230 240 250
    NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
    260 270 280 290 300
    MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
    310 320 330 340 350
    LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV
    360 370 380 390 400
    ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV
    410 420 430 440 450
    PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
    460 470 480 490 500
    DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ
    510 520 530 540 550
    HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL
    560 570 580
    GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
    Length:585
    Mass (Da):65,411
    Last modified:February 1, 1996 - v1
    Checksum:i0322509F0E2C32EE
    GO

    Sequence cautioni

    The sequence CAA49554 differs from that shown. Reason: Erroneous initiation.Curated

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_01882112G → R1 PublicationCorresponds to variant dbSNP:rs8190591Ensembl.1
    Natural variantiVAR_018822124K → N1 PublicationCorresponds to variant dbSNP:rs8190600Ensembl.1
    Natural variantiVAR_029176153P → Q. Corresponds to variant dbSNP:rs2839672Ensembl.1
    Natural variantiVAR_029177232G → E. Corresponds to variant dbSNP:rs2839673Ensembl.1
    Natural variantiVAR_018823286K → R1 PublicationCorresponds to variant dbSNP:rs8190671Ensembl.1
    Natural variantiVAR_029178326G → A. Corresponds to variant dbSNP:rs2839678Ensembl.1
    Natural variantiVAR_018824375R → Q1 PublicationCorresponds to variant dbSNP:rs8190730Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M81882 mRNA Translation: AAA62367.1
    M74826 mRNA Translation: AAA58491.1
    AY340073 Genomic DNA Translation: AAP88040.1
    X69936 mRNA Translation: CAA49554.1 Different initiation.
    M70435 mRNA Translation: AAA52513.1
    CCDSiCCDS7149.1
    PIRiA41935 A41292
    RefSeqiNP_000809.1, NM_000818.2
    NP_001127838.1, NM_001134366.1
    UniGeneiHs.231829

    Genome annotation databases

    EnsembliENST00000259271; ENSP00000259271; ENSG00000136750
    ENST00000376261; ENSP00000365437; ENSG00000136750
    GeneIDi2572
    KEGGihsa:2572

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiDCE2_HUMAN
    AccessioniPrimary (citable) accession number: Q05329
    Secondary accession number(s): Q9UD87
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: July 18, 2018
    This is version 158 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

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