MXD1

Functionⁱ

Transcriptional repressor. MAD binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MAD thus antagonizes MYC transcriptional activity by competing for MAX.

GO - Molecular functionⁱ

DNA-binding transcription factor activity
Source: ProtInc
Traceable author statementⁱ
- 7829091
- 8425218
DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
protein dimerization activity Source: InterPro
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- 10723141
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 10723141
transcription coregulator activity
Source: ProtInc
Traceable author statementⁱ
- 7829091
transcription corepressor activity
Source: ProtInc
Traceable author statementⁱ
- 8425218

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell proliferation
Source: ProtInc
Traceable author statementⁱ
- 7829091
multicellular organism development
Source: ProtInc
Traceable author statementⁱ
- 7829091
negative regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 10723141

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

SIGNORⁱ	Q05195

SIGNORⁱ

Q05195

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Max dimerization protein 1 Short name: Max dimerizer 1 Alternative name(s): Protein MAD
Gene namesⁱ	Name:MXD1 Synonyms:MAD
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 2

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000059728.10
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:6761 MXD1
MIMⁱ	600021 gene
neXtProtⁱ	NX_Q05195

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	4084
Open Targets More...OpenTargetsⁱ	ENSG00000059728
PharmGKBⁱ	PA30520

Polymorphism and mutation databases

BioMutaⁱ	MXD1
DMDMⁱ	729978

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127264	1 – 221	Max dimerization protein 1Add BLAST		221

Post-translational modificationⁱ

Ubiquitinated by BIRC2/c-IAP1, leading to its subsequent degradation by the proteasome.1 Publication

Keywords - PTMⁱ

Ubl conjugation

Proteomic databases

PaxDbⁱ	Q05195
PeptideAtlas More...PeptideAtlasⁱ	Q05195
PRIDEⁱ	Q05195
ProteomicsDBⁱ	58314 58315 [Q05195-2]

PTM databases

iPTMnetⁱ	Q05195
PhosphoSitePlusⁱ	Q05195

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000059728 Expressed in 205 organ(s), highest expression level in buccal mucosa cell
CleanExⁱ	HS_MXD1
ExpressionAtlasⁱ	Q05195 baseline and differential
Genevisibleⁱ	Q05195 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA001599

HPAⁱ

HPA001599

Interactionⁱ

Subunit structureⁱ

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with RNF17 (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
MAX	P61244	3	EBI-8833637,EBI-751711

With

Entry

#Exp.

IntAct

Notes

MAX

P61244

3

EBI-8833637,EBI-751711

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	110259, 37 interactors
ComplexPortalⁱ	CPX-104 Transcriptional repressor Mad-Max complex
CORUMⁱ	Q05195
Database of interacting proteins More...DIPⁱ	DIP-204N
ELMⁱ	Q05195
IntActⁱ	Q05195, 6 interactors
STRINGⁱ	9606.ENSP00000264444

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	9 – 19	Combined sources	11
Helixⁱ	58 – 81	Combined sources	24
Beta strandⁱ	87 – 89	Combined sources	3
Helixⁱ	95 – 134	Combined sources	40

Show more details

3D structure databases

ProteinModelPortalⁱ	Q05195
SMRⁱ	Q05195
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q05195

EvolutionaryTraceⁱ

Q05195

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	56 – 108	bHLHPROSITE-ProRule annotationAdd BLAST		53

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	21 – 49	Nuclear localization signalSequence analysisAdd BLAST		29

Phylogenomic databases

eggNOGⁱ	ENOG410IPD8 Eukaryota ENOG410XSF5 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046360
HOGENOMⁱ	HOG000247060
HOVERGENⁱ	HBG006314
InParanoidⁱ	Q05195
KEGG Orthology (KO) More...KOⁱ	K09114
OMAⁱ	DNHKTCL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0QBK
PhylomeDBⁱ	Q05195
TreeFamⁱ	TF315654

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q05195-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAVRMNIQ MLLEAADYLE RREREAEHGY ASMLPYNNKD RDALKRRNKS 
        60         70         80         90        100
KKNNSSSRST HNEMEKNRRA HLRLCLEKLK GLVPLGPESS RHTTLSLLTK 
       110        120        130        140        150
AKLHIKKLED CDRKAVHQID QLQREQRHLK RQLEKLGIER IRMDSIGSTV 
       160        170        180        190        200
SSERSDSDRE EIDVDVESTD YLTGDLDWSS SSVSDSDERG SMQSLGSDEG 
       210        220 
YSSTSIKRIK LQDSHKACLG L

Length:221

Mass (Da):25,254

Last modified:February 1, 1995 - v1

Checksum:ⁱB39FAEBFD708B6AB

GO

Isoform 2 (identifier: Q05195-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
58-67: Missing.

« Hide

        10         20         30         40         50
MAAAVRMNIQ MLLEAADYLE RREREAEHGY ASMLPYNNKD RDALKRRNKS 
        60         70         80         90        100
KKNNSSSRRA HLRLCLEKLK GLVPLGPESS RHTTLSLLTK AKLHIKKLED 
       110        120        130        140        150
CDRKAVHQID QLQREQRHLK RQLEKLGIER IRMDSIGSTV SSERSDSDRE 
       160        170        180        190        200
EIDVDVESTD YLTGDLDWSS SSVSDSDERG SMQSLGSDEG YSSTSIKRIK 
       210 
LQDSHKACLG L

Note: No experimental confirmation available.

Show »

Length:211

Mass (Da):24,027

Checksum:ⁱ0C30C9CD1A02730B

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

C9JBE8	C9JBE8_HUMAN	Max dimerization protein 1 Max dimerization protein 1	MXD1	181	Annotation score:
F8WBI0	F8WBI0_HUMAN	Max dimerization protein 1 Max dimerization protein 1	MXD1	73	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_043074	58 – 67	Missing in isoform 2. 1 Publication		10

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L06895 mRNA Translation: AAA36194.1 CR536495 mRNA Translation: CAG38734.1 CR541692 mRNA Translation: CAG46493.1 AK312734 mRNA Translation: BAG35605.1 AC019206 Genomic DNA Translation: AAY14867.1 CH471053 Genomic DNA Translation: EAW99839.1 BC069377 mRNA Translation: AAH69377.1 BC069433 mRNA Translation: AAH69433.1 BC098396 mRNA Translation: AAH98396.1 BC113531 mRNA Translation: AAI13532.1 BC117260 mRNA Translation: AAI17261.1 BC143831 mRNA Translation: AAI43832.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS1896.1 [Q05195-1] CCDS56123.1 [Q05195-2]
PIRⁱ	A45181
NCBI Reference Sequences More...RefSeqⁱ	NP_001189442.1, NM_001202513.1 NP_001189443.1, NM_001202514.1 [Q05195-2] NP_002348.1, NM_002357.3 [Q05195-1]
UniGeneⁱ	Hs.468908

Genome annotation databases

Ensemblⁱ	ENST00000264444; ENSP00000264444; ENSG00000059728 [Q05195-1] ENST00000540449; ENSP00000443935; ENSG00000059728 [Q05195-2]
GeneIDⁱ	4084
KEGGⁱ	hsa:4084
UCSC genome browser More...UCSCⁱ	uc002sfy.4 human [Q05195-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q05195	MAX dimerization protein 1		PANTR		221	UniRef100_Q05195
Uncharacterized protein		PANPA		221
MAX dimerization protein 1		GORGO		221
Max dimerization protein 1 (Fragment)		HUMAN		181
MXD1 isoform 1 (Fragment)		PANTR		181
Q05195-2	MAX dimerization protein 1		PANTR		211	UniRef100_A0A2I3TGR2
Uncharacterized protein		PANPA		211
MAX dimerization protein 1		GORGO		211

Protein	Similar proteins		Species	Score	Length	Source
Q05195	MAX dimerization protein 1		PANTR		221	UniRef90_Q05195
Uncharacterized protein		PANPA		221
MAX dimerization protein 1		GORGO		221
Max dimerization protein 1 (Fragment)		HUMAN		181
Uncharacterized protein		MYOLU		233
+113
Q05195-2	Uncharacterized protein		RABIT		229	UniRef90_G1TXD0
Uncharacterized protein		PROCO		211
Uncharacterized protein		FELCA		211
max dimerization protein 1 isoform X3		TARSY		211
Uncharacterized protein		RHIRO		211
+33

Protein	Similar proteins		Species	Score	Length	Source
Q05195	Max dimerization protein 1		XENLA		221	UniRef50_Q05195
Max dimerization protein 1		XENTR		221
Max dimerization protein 1		MOUSE		227
MAX dimerization protein 1		PANTR		221
Uncharacterized protein		PANPA		221
+379

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L06895 mRNA Translation: AAA36194.1 CR536495 mRNA Translation: CAG38734.1 CR541692 mRNA Translation: CAG46493.1 AK312734 mRNA Translation: BAG35605.1 AC019206 Genomic DNA Translation: AAY14867.1 CH471053 Genomic DNA Translation: EAW99839.1 BC069377 mRNA Translation: AAH69377.1 BC069433 mRNA Translation: AAH69433.1 BC098396 mRNA Translation: AAH98396.1 BC113531 mRNA Translation: AAI13532.1 BC117260 mRNA Translation: AAI17261.1 BC143831 mRNA Translation: AAI43832.1
CCDSⁱ	CCDS1896.1 [Q05195-1] CCDS56123.1 [Q05195-2]
PIRⁱ	A45181
RefSeqⁱ	NP_001189442.1, NM_001202513.1 NP_001189443.1, NM_001202514.1 [Q05195-2] NP_002348.1, NM_002357.3 [Q05195-1]
UniGeneⁱ	Hs.468908

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1E91	NMR	-	B	8-20	[»]
	1G1E	NMR	-	A	6-21	[»]
	1NLW	X-ray	2.00	A/D	57-136	[»]
	1PD7	NMR	-	B	5-28	[»]
	1S5Q	NMR	-	A	6-21	[»]
ProteinModelPortalⁱ	Q05195
SMRⁱ	Q05195
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	110259, 37 interactors
ComplexPortalⁱ	CPX-104 Transcriptional repressor Mad-Max complex
CORUMⁱ	Q05195
DIPⁱ	DIP-204N
ELMⁱ	Q05195
IntActⁱ	Q05195, 6 interactors
STRINGⁱ	9606.ENSP00000264444

PTM databases

iPTMnetⁱ	Q05195
PhosphoSitePlusⁱ	Q05195

Polymorphism and mutation databases

BioMutaⁱ	MXD1
DMDMⁱ	729978

Proteomic databases

PaxDbⁱ	Q05195
PeptideAtlasⁱ	Q05195
PRIDEⁱ	Q05195
ProteomicsDBⁱ	58314 58315 [Q05195-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	4084
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000264444; ENSP00000264444; ENSG00000059728 [Q05195-1] ENST00000540449; ENSP00000443935; ENSG00000059728 [Q05195-2]
GeneIDⁱ	4084
KEGGⁱ	hsa:4084
UCSCⁱ	uc002sfy.4 human [Q05195-1]

Organism-specific databases

CTDⁱ	4084
DisGeNETⁱ	4084
EuPathDBⁱ	HostDB:ENSG00000059728.10
GeneCardsⁱ	MXD1
HGNCⁱ	HGNC:6761 MXD1
HPAⁱ	HPA001599
MIMⁱ	600021 gene
neXtProtⁱ	NX_Q05195
OpenTargetsⁱ	ENSG00000059728
PharmGKBⁱ	PA30520
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IPD8 Eukaryota ENOG410XSF5 LUCA
GeneTreeⁱ	ENSGT00510000046360
HOGENOMⁱ	HOG000247060
HOVERGENⁱ	HBG006314
InParanoidⁱ	Q05195
KOⁱ	K09114
OMAⁱ	DNHKTCL
OrthoDBⁱ	EOG091G0QBK
PhylomeDBⁱ	Q05195
TreeFamⁱ	TF315654

Enzyme and pathway databases

SIGNORⁱ	Q05195

SIGNORⁱ

Q05195

Miscellaneous databases

ChiTaRSⁱ	MXD1 human
EvolutionaryTraceⁱ	Q05195
GeneWikiⁱ	MXD1
GenomeRNAiⁱ	4084
Protein Ontology More...PROⁱ	PR:Q05195
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000059728 Expressed in 205 organ(s), highest expression level in buccal mucosa cell
CleanExⁱ	HS_MXD1
ExpressionAtlasⁱ	Q05195 baseline and differential
Genevisibleⁱ	Q05195 HS

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAD1_HUMAN
Accessionⁱ	Q05195Primary (citable) accession number: Q05195 Secondary accession number(s): B2R6V8 , B7ZLI6, D6W5G2, Q6FI41
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
	Last sequence update:	February 1, 1995
	Last modified:	November 7, 2018
	This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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Supporting data

UniProtKB - Q05195 (MAD1_HUMAN)

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Max dimerization protein 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytosol

Mitochondrion

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ