UniProtKB - Q05195 (MAD1_HUMAN)
Max dimerization protein 1
MXD1
Functioni
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SB
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q05195 |
SIGNORi | Q05195 |
Names & Taxonomyi
Protein namesi | Recommended name: Max dimerization protein 1Short name: Max dimerizer 1 Alternative name(s): Protein MAD |
Gene namesi | Name:MXD1 Synonyms:MAD |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6761, MXD1 |
MIMi | 600021, gene |
neXtProti | NX_Q05195 |
VEuPathDBi | HostDB:ENSG00000059728.10 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: HPA
Mitochondrion
- mitochondrion Source: HPA
Nucleus
- nuclear chromatin Source: BHF-UCL
- nucleoplasm Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4084 |
OpenTargetsi | ENSG00000059728 |
PharmGKBi | PA30520 |
Miscellaneous databases
Pharosi | Q05195, Tbio |
Genetic variation databases
BioMutai | MXD1 |
DMDMi | 729978 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127264 | 1 – 221 | Max dimerization protein 1Add BLAST | 221 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
MassIVEi | Q05195 |
PaxDbi | Q05195 |
PeptideAtlasi | Q05195 |
PRIDEi | Q05195 |
ProteomicsDBi | 58314 [Q05195-1] 58315 [Q05195-2] |
PTM databases
iPTMneti | Q05195 |
PhosphoSitePlusi | Q05195 |
Expressioni
Gene expression databases
Bgeei | ENSG00000059728, Expressed in buccal mucosa cell and 219 other tissues |
ExpressionAtlasi | Q05195, baseline and differential |
Genevisiblei | Q05195, HS |
Organism-specific databases
HPAi | ENSG00000059728, Tissue enhanced (blood, bone marrow, esophagus) |
Interactioni
Subunit structurei
Heterodimer with MAX; the interaction is required for DNA-binding (PubMed:8425218). DNA binding requires dimerization with another bHLH protein; does not form homodimers, and does not bind to DNA in the absence of MAX in vitro (PubMed:8425218).
Interacts with RNF17 (By similarity).
By similarity1 PublicationBinary interactionsi
Q05195
With | #Exp. | IntAct |
---|---|---|
MAX [P61244] | 3 | EBI-8833637,EBI-751711 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 110259, 38 interactors |
ComplexPortali | CPX-104, Transcriptional repressor Mad-Max complex |
CORUMi | Q05195 |
DIPi | DIP-204N |
ELMi | Q05195 |
IntActi | Q05195, 14 interactors |
STRINGi | 9606.ENSP00000264444 |
Miscellaneous databases
RNActi | Q05195, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q05195 |
SMRi | Q05195 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q05195 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 108 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 21 – 49 | Nuclear localization signalSequence analysisAdd BLAST | 29 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000159446 |
HOGENOMi | CLU_082604_0_1_1 |
InParanoidi | Q05195 |
OMAi | SERKAQH |
OrthoDBi | 1311585at2759 |
PhylomeDBi | Q05195 |
TreeFami | TF315654 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
IDEALi | IID00165 |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040157, MXD1 |
PANTHERi | PTHR11969:SF18, PTHR11969:SF18, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAVRMNIQ MLLEAADYLE RREREAEHGY ASMLPYNNKD RDALKRRNKS
60 70 80 90 100
KKNNSSSRST HNEMEKNRRA HLRLCLEKLK GLVPLGPESS RHTTLSLLTK
110 120 130 140 150
AKLHIKKLED CDRKAVHQID QLQREQRHLK RQLEKLGIER IRMDSIGSTV
160 170 180 190 200
SSERSDSDRE EIDVDVESTD YLTGDLDWSS SSVSDSDERG SMQSLGSDEG
210 220
YSSTSIKRIK LQDSHKACLG L
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JBE8 | C9JBE8_HUMAN | Max dimerization protein 1 | MXD1 | 181 | Annotation score: | ||
F8WBI0 | F8WBI0_HUMAN | Max dimerization protein 1 | MXD1 | 73 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_043074 | 58 – 67 | Missing in isoform 2. 1 Publication | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06895 mRNA Translation: AAA36194.1 CR536495 mRNA Translation: CAG38734.1 CR541692 mRNA Translation: CAG46493.1 AK312734 mRNA Translation: BAG35605.1 AC019206 Genomic DNA Translation: AAY14867.1 CH471053 Genomic DNA Translation: EAW99839.1 BC069377 mRNA Translation: AAH69377.1 BC069433 mRNA Translation: AAH69433.1 BC098396 mRNA Translation: AAH98396.1 BC113531 mRNA Translation: AAI13532.1 BC117260 mRNA Translation: AAI17261.1 BC143831 mRNA Translation: AAI43832.1 |
CCDSi | CCDS1896.1 [Q05195-1] CCDS56123.1 [Q05195-2] |
PIRi | A45181 |
RefSeqi | NP_001189442.1, NM_001202513.1 NP_001189443.1, NM_001202514.1 [Q05195-2] NP_002348.1, NM_002357.3 [Q05195-1] |
Genome annotation databases
Ensembli | ENST00000264444; ENSP00000264444; ENSG00000059728 [Q05195-1] ENST00000540449; ENSP00000443935; ENSG00000059728 [Q05195-2] |
GeneIDi | 4084 |
KEGGi | hsa:4084 |
UCSCi | uc002sfy.4, human [Q05195-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06895 mRNA Translation: AAA36194.1 CR536495 mRNA Translation: CAG38734.1 CR541692 mRNA Translation: CAG46493.1 AK312734 mRNA Translation: BAG35605.1 AC019206 Genomic DNA Translation: AAY14867.1 CH471053 Genomic DNA Translation: EAW99839.1 BC069377 mRNA Translation: AAH69377.1 BC069433 mRNA Translation: AAH69433.1 BC098396 mRNA Translation: AAH98396.1 BC113531 mRNA Translation: AAI13532.1 BC117260 mRNA Translation: AAI17261.1 BC143831 mRNA Translation: AAI43832.1 |
CCDSi | CCDS1896.1 [Q05195-1] CCDS56123.1 [Q05195-2] |
PIRi | A45181 |
RefSeqi | NP_001189442.1, NM_001202513.1 NP_001189443.1, NM_001202514.1 [Q05195-2] NP_002348.1, NM_002357.3 [Q05195-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E91 | NMR | - | B | 8-20 | [»] | |
1G1E | NMR | - | A | 6-21 | [»] | |
1NLW | X-ray | 2.00 | A/D | 57-136 | [»] | |
1PD7 | NMR | - | B | 5-28 | [»] | |
1S5Q | NMR | - | A | 6-21 | [»] | |
BMRBi | Q05195 | |||||
SMRi | Q05195 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110259, 38 interactors |
ComplexPortali | CPX-104, Transcriptional repressor Mad-Max complex |
CORUMi | Q05195 |
DIPi | DIP-204N |
ELMi | Q05195 |
IntActi | Q05195, 14 interactors |
STRINGi | 9606.ENSP00000264444 |
PTM databases
iPTMneti | Q05195 |
PhosphoSitePlusi | Q05195 |
Genetic variation databases
BioMutai | MXD1 |
DMDMi | 729978 |
Proteomic databases
MassIVEi | Q05195 |
PaxDbi | Q05195 |
PeptideAtlasi | Q05195 |
PRIDEi | Q05195 |
ProteomicsDBi | 58314 [Q05195-1] 58315 [Q05195-2] |
Protocols and materials databases
Antibodypediai | 893, 176 antibodies |
DNASUi | 4084 |
Genome annotation databases
Ensembli | ENST00000264444; ENSP00000264444; ENSG00000059728 [Q05195-1] ENST00000540449; ENSP00000443935; ENSG00000059728 [Q05195-2] |
GeneIDi | 4084 |
KEGGi | hsa:4084 |
UCSCi | uc002sfy.4, human [Q05195-1] |
Organism-specific databases
CTDi | 4084 |
DisGeNETi | 4084 |
GeneCardsi | MXD1 |
HGNCi | HGNC:6761, MXD1 |
HPAi | ENSG00000059728, Tissue enhanced (blood, bone marrow, esophagus) |
MIMi | 600021, gene |
neXtProti | NX_Q05195 |
OpenTargetsi | ENSG00000059728 |
PharmGKBi | PA30520 |
VEuPathDBi | HostDB:ENSG00000059728.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000159446 |
HOGENOMi | CLU_082604_0_1_1 |
InParanoidi | Q05195 |
OMAi | SERKAQH |
OrthoDBi | 1311585at2759 |
PhylomeDBi | Q05195 |
TreeFami | TF315654 |
Enzyme and pathway databases
PathwayCommonsi | Q05195 |
SIGNORi | Q05195 |
Miscellaneous databases
BioGRID-ORCSi | 4084, 1 hit in 896 CRISPR screens |
ChiTaRSi | MXD1, human |
EvolutionaryTracei | Q05195 |
GeneWikii | MXD1 |
GenomeRNAii | 4084 |
Pharosi | Q05195, Tbio |
PROi | PR:Q05195 |
RNActi | Q05195, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000059728, Expressed in buccal mucosa cell and 219 other tissues |
ExpressionAtlasi | Q05195, baseline and differential |
Genevisiblei | Q05195, HS |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
IDEALi | IID00165 |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR040157, MXD1 |
PANTHERi | PTHR11969:SF18, PTHR11969:SF18, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAD1_HUMAN | |
Accessioni | Q05195Primary (citable) accession number: Q05195 Secondary accession number(s): B2R6V8 Q6FI41 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM