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Protein

Clathrin coat assembly protein AP180

Gene

Snap91

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: InterPro
  • clathrin adaptor activity Source: CAFA
  • clathrin heavy chain binding Source: CAFA
  • inositol hexakisphosphate binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  • phospholipase binding Source: RGD
  • SH2 domain binding Source: RGD
  • SNARE binding Source: RGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin coat assembly protein AP180
Alternative name(s):
91 kDa synaptosomal-associated protein
Clathrin coat-associated protein AP180
Gene namesi
Name:Snap91
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69276 Snap91

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001938661 – 915Clathrin coat assembly protein AP180Add BLAST915

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineCombined sources1
Modified residuei300PhosphoserineCombined sources1
Modified residuei306Phosphoserine1 Publication1
Glycosylationi310O-linked (GlcNAc) threonine1 Publication1
Modified residuei313PhosphoserineCombined sources1 Publication1
Modified residuei317PhosphothreonineBy similarity1
Modified residuei594PhosphoserineCombined sources1
Modified residuei600Phosphoserine1 Publication1
Modified residuei640Phosphoserine1 Publication1
Modified residuei646Phosphoserine1 Publication1
Modified residuei775PhosphoserineCombined sources1
Modified residuei873Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei873Omega-N-methylarginine; alternateBy similarity1
Isoform 2 (identifier: Q05140-2)
Modified residuei600PhosphoserineCombined sources1
Modified residuei627PhosphoserineCombined sources1

Post-translational modificationi

Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. The form with phosphorylated O-linked N-acetylglucosamine is predominant in brain synaptosomes. There is no evidence for direct Thr-310 phosphorylation (PubMed:21500857).1 Publication

Keywords - PTMi

Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ05140
PRIDEiQ05140

PTM databases

iPTMnetiQ05140
PhosphoSitePlusiQ05140
SwissPalmiQ05140

Interactioni

Subunit structurei

Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249290, 7 interactors
CORUMiQ05140
ELMiQ05140
IntActiQ05140, 4 interactors
MINTiQ05140
STRINGi10116.ENSRNOP00000030821

Structurei

3D structure databases

DisProtiDP00225
ProteinModelPortaliQ05140
SMRiQ05140
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 145ENTHPROSITE-ProRule annotationAdd BLAST132

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi410 – 413Poly-Thr4
Compositional biasi535 – 539Poly-Ala5
Compositional biasi547 – 550Poly-Ala4
Compositional biasi678 – 683Poly-Ser6
Compositional biasi723 – 729Poly-Ser7

Domaini

Possesses a three domain structure: the N-terminal 300 residues harbor a clathrin binding site, an acidic middle domain 450 residues, interrupted by an Ala-rich segment, and the C-terminal domain (166 residues).

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
HOGENOMiHOG000015763
HOVERGENiHBG049391
InParanoidiQ05140
KOiK20043
PhylomeDBiQ05140

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q05140-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN
60 70 80 90 100
ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK
160 170 180 190 200
GADGVMRTMV PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL
210 220 230 240 250
FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF
260 270 280 290 300
LKVADEVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS
310 320 330 340 350
PLSKSSPATT VTSPNSTPAK TIDTSPPVDI FATASAAAPV SSAKPSSDLL
360 370 380 390 400
DLQPDFSGAR AGAAAPVPPP TGGATAWGDL LGEDSLAALS SVPSEAPISD
410 420 430 440 450
PFAPEPSPPT TTTEPASASA SATTAVTAAT TEVDLFGDAF AASPGEAPAA
460 470 480 490 500
SEGATAPATP APVAAALDAC SGNDPFAPSE GSAEAAPELD LFAMKPPETS
510 520 530 540 550
APVVTPTAST APPVPATAPS PAPTAVAATA ATTTAAAAAT TTATTSAAAA
560 570 580 590 600
TTAAAPPALD IFGDLFDSAP EVAAASKPDV APSIDLFGTD AFSSPPRGAS
610 620 630 640 650
PVPESSLTAD LLSGSGFHCA EDDRHVPLFF TAVDAFAAPS PASTASPAKA
660 670 680 690 700
ESSGVIDLFG DAFGSSASET QPAPQAVSSS SASADLLAGF GGSFMAPSTT
710 720 730 740 750
PVTPAQNNLL QPNFEAAFGT TPSTSSSSSF DPSGDLLMPT MAPSGQPAPV
760 770 780 790 800
SMVPPSPAMS ASKGLGSDLD SSLASLVGNL GISGTTSKKG DLQWNAGEKK
810 820 830 840 850
LTGGANWQPK VTPATWSAGV PPQGTVPPTS SVPPGAGAPS VGQPGAGYGM
860 870 880 890 900
PPAGTGMTMM PQQPVMFAQP MMRPPFGAAA VPGTQLSPSP TPATQSPKKP
910
PAKDPLADLN IKDFL
Length:915
Mass (Da):93,519
Last modified:November 1, 1996 - v1
Checksum:i32EC1B38EC5DF8C0
GO
Isoform 2 (identifier: Q05140-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     614-632: Missing.

Show »
Length:896
Mass (Da):91,431
Checksum:i41417E79D1044984
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1LRK0F1LRK0_RAT
Clathrin coat assembly protein AP18...
Snap91
918Annotation score:
A0A0G2K0B6A0A0G2K0B6_RAT
Clathrin coat assembly protein AP18...
Snap91
897Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000173614 – 632Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68877 mRNA Translation: CAA48748.1
X68878 mRNA Translation: CAA48749.1
PIRiS36326
S36327
RefSeqiNP_113916.1, NM_031728.1 [Q05140-1]
UniGeneiRn.11022
Rn.164718

Genome annotation databases

GeneIDi65178
KEGGirno:65178
UCSCiRGD:69276 rat [Q05140-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68877 mRNA Translation: CAA48748.1
X68878 mRNA Translation: CAA48749.1
PIRiS36326
S36327
RefSeqiNP_113916.1, NM_031728.1 [Q05140-1]
UniGeneiRn.11022
Rn.164718

3D structure databases

DisProtiDP00225
ProteinModelPortaliQ05140
SMRiQ05140
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249290, 7 interactors
CORUMiQ05140
ELMiQ05140
IntActiQ05140, 4 interactors
MINTiQ05140
STRINGi10116.ENSRNOP00000030821

PTM databases

iPTMnetiQ05140
PhosphoSitePlusiQ05140
SwissPalmiQ05140

Proteomic databases

PaxDbiQ05140
PRIDEiQ05140

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65178
KEGGirno:65178
UCSCiRGD:69276 rat [Q05140-1]

Organism-specific databases

CTDi9892
RGDi69276 Snap91

Phylogenomic databases

eggNOGiKOG0251 Eukaryota
ENOG410XQ90 LUCA
HOGENOMiHOG000015763
HOVERGENiHBG049391
InParanoidiQ05140
KOiK20043
PhylomeDBiQ05140

Miscellaneous databases

PROiPR:Q05140

Family and domain databases

Gene3Di1.20.58.150, 1 hit
1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR011417 ANTH_dom
IPR014712 Clathrin_AP_dom2
IPR013809 ENTH
IPR008942 ENTH_VHS
PfamiView protein in Pfam
PF07651 ANTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAP180_RAT
AccessioniPrimary (citable) accession number: Q05140
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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