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Protein

Matrix protein VP40

Gene

VP40

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication.2 Publications

Miscellaneous

Most abundant protein in the virion.

GO - Molecular functioni

GO - Biological processi

  • intracellular transport of virus Source: CACAO
  • suppression of host defenses Source: CACAO
  • viral budding Source: CACAO
  • viral budding from plasma membrane Source: UniProtKB
  • viral budding via host ESCRT complex Source: UniProtKB-KW

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell, Viral RNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein VP40
Alternative name(s):
Membrane-associated protein VP40
Gene namesi
Name:VP40
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Proteomesi
  • UP000007209 Componenti: Genome
  • UP000109874 Componenti: Genome
  • UP000149419 Componenti: Genome
  • UP000150973 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7P → A: Partial loss of budding. 1 Publication1
Mutagenesisi10 – 11PP → AA: 90% loss of budding. 1 Publication2
Mutagenesisi11P → A: Complete loss of budding. 1
Mutagenesisi13Y → A: Complete loss of interaction with WW domain containing proteins. 1 Publication1
Mutagenesisi125F → A: Partial loss of RNA-binding. Complete loss of virus infectivity. 1 Publication1
Mutagenesisi134R → A: Complete loss of RNA-binding. Complete loss of virus infectivity. 1 Publication1
Mutagenesisi212 – 214KLR → AAA: 85% loss of budding efficiency. Impaired oligomerization. 1 Publication3
Mutagenesisi212 – 214KLR → ALA: 80% loss of budding efficiency. No effect on oligomerization. 1 Publication3
Mutagenesisi212 – 213KL → AA: 84% loss of budding efficiency. Impaired oligomerization. 2
Mutagenesisi212K → A: 40% loss of budding efficiency. No effect on oligomerization. 1
Mutagenesisi213 – 214LR → AA: 84% loss of budding efficiency. Impaired oligomerization. 2
Mutagenesisi213L → A: 87% loss of budding efficiency. Impaired oligomerization. 1
Mutagenesisi213L → I: 40% loss of budding efficiency. 1
Mutagenesisi214R → A: 65% loss of budding efficiency. No effect on oligomerization. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002221641 – 326Matrix protein VP40Add BLAST326

Interactioni

Subunit structurei

Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35.5 Publications

Protein-protein interaction databases

ELMiQ05128

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ05128
SMRiQ05128
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ05128

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 214Important for oligomerization3
Regioni213 – 326Membrane-bindingBy similarityAdd BLAST114

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 10PTAP/PSAP motif4
Motifi10 – 13PPXY motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000FQ

Family and domain databases

Gene3Di2.60.510.10, 1 hit
InterProiView protein in InterPro
IPR008986 EV_matrix
IPR035092 EV_matrix_protein_C-terminal
IPR038057 EV_matrix_sf
PfamiView protein in Pfam
PF07447 VP40, 1 hit
PIRSFiPIRSF018327 VP40_FiloV, 1 hit
SUPFAMiSSF50012 SSF50012, 2 hits

Sequencei

Sequence statusi: Complete.

Q05128-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP
60 70 80 90 100
LRPIADDTID HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV
110 120 130 140 150
ADQKTYSFDS TTAAIMLASY TITHFGKATN PLVRVNRLGP GIPDHPLRLL
160 170 180 190 200
RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK LITQPLPAAT WTDDTPTGSN
210 220 230 240 250
GALRPGISFH PKLRPILLPN KSGKKGNSAD LTSPEKIQAI MTSLQDFKIV
260 270 280 290 300
PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP
310 320
GDLTMVITQD CDTCHSPASL PAVIEK
Length:326
Mass (Da):35,183
Last modified:February 1, 1994 - v1
Checksum:i1AB132C0DB8E9003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61274 Genomic RNA Translation: CAA43579.1
L11365 Genomic RNA Translation: AAB81003.1
AF086833 Genomic RNA Translation: AAD14583.1
AF272001 Genomic RNA Translation: AAG40166.1
AF499101 Genomic RNA Translation: AAM76033.1
AY142960 Genomic RNA Translation: AAN37506.1
RefSeqiNP_066245.1, NC_002549.1

Genome annotation databases

GeneIDi911825
KEGGivg:911825

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61274 Genomic RNA Translation: CAA43579.1
L11365 Genomic RNA Translation: AAB81003.1
AF086833 Genomic RNA Translation: AAD14583.1
AF272001 Genomic RNA Translation: AAG40166.1
AF499101 Genomic RNA Translation: AAM76033.1
AY142960 Genomic RNA Translation: AAN37506.1
RefSeqiNP_066245.1, NC_002549.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H2CX-ray1.60A55-194[»]
1H2DX-ray2.60A/B31-212[»]
2KQ0NMR-B5-16[»]
4EJEX-ray2.20C/D5-13[»]
4LDBX-ray3.10A/B/C/D44-326[»]
4LDDX-ray3.50A/B/C44-326[»]
4LDIX-ray4.15A/B44-326[»]
4LDMX-ray1.85A44-188[»]
ProteinModelPortaliQ05128
SMRiQ05128
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiQ05128

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911825
KEGGivg:911825

Phylogenomic databases

OrthoDBiVOG090000FQ

Miscellaneous databases

EvolutionaryTraceiQ05128

Family and domain databases

Gene3Di2.60.510.10, 1 hit
InterProiView protein in InterPro
IPR008986 EV_matrix
IPR035092 EV_matrix_protein_C-terminal
IPR038057 EV_matrix_sf
PfamiView protein in Pfam
PF07447 VP40, 1 hit
PIRSFiPIRSF018327 VP40_FiloV, 1 hit
SUPFAMiSSF50012 SSF50012, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiVP40_EBOZM
AccessioniPrimary (citable) accession number: Q05128
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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