UniProtKB - Q05127 (VP35_EBOZM)
Polymerase cofactor VP35
VP35
Functioni
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- negative regulation of gene expression Source: CACAO
- negative regulation of gene silencing by miRNA Source: CACAO
- positive regulation of protein sumoylation Source: CACAO
- suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II Source: CACAO
- suppression by virus of host cytokine production Source: CACAO
- suppression by virus of host IKBKE activity Source: UniProtKB-KW
- suppression by virus of host IRF7 activity Source: UniProtKB-KW
- suppression by virus of host protein phosphorylation Source: CACAO
- suppression by virus of host TBK1 activity Source: UniProtKB-KW
- suppression by virus of host toll-like receptor signaling pathway Source: UniProtKB-KW
- suppression by virus of host type I interferon production Source: CACAO
- suppression of host defenses by symbiont Source: CACAO
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Polymerase cofactor VP35Alternative name(s): Ebola VP351 Publication Short name: eVP351 Publication |
Gene namesi | Name:VP35 |
Organismi | Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) |
Taxonomic identifieri | 128952 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Haploviricotina › Monjiviricetes › Mononegavirales › Filoviridae › Ebolavirus › |
Virus hosti | Epomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231] Homo sapiens (Human) [TaxID: 9606] Myonycteris torquata (Little collared fruit bat) [TaxID: 77243] |
Proteomesi |
|
Subcellular locationi
- Virion
- Host cytoplasm 2 Publications
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-SubCell
- viral nucleocapsid Source: CACAO
Keywords - Cellular componenti
Host cytoplasm, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 – 93 | LASL → AASA: Complete loss of homotrimerization; when associated with A-107. 1 Publication | 4 | |
Mutagenesisi | 107 | L → A: Complete loss of homotrimerization; when associated with 90-AASA-93. 1 Publication | 1 | |
Mutagenesisi | 187 | S → A: Impaired viral replication. 1 Publication | 1 | |
Mutagenesisi | 187 | S → D: No effect on viral replication. 1 Publication | 1 | |
Mutagenesisi | 210 | T → A: Loss of viral transcription and reduced binding to the nucleoprotein. 1 Publication | 1 | |
Mutagenesisi | 210 | T → D: No effect on viral transcription and binding to the nucleoprotein. 1 Publication | 1 | |
Mutagenesisi | 239 | F → A: Complete loss of interaction with host PRKRA and subsequent immune response inhibition. 1 Publication | 1 | |
Mutagenesisi | 305 | R → A: No effect on IRF3 promoter inhibition. 1 Publication | 1 | |
Mutagenesisi | 309 | K → A: Partial loss of IRF3 promoter inhibition. Complete loss of dsRNA-binding. 2 Publications | 1 | |
Mutagenesisi | 312 | R → A: Complete loss of IRF3 promoter inhibition; dsRNA-binding and interaction with host PRKRA. 3 Publications | 1 | |
Mutagenesisi | 317 | S → A: Impaired viral replication. 1 Publication | 1 | |
Mutagenesisi | 317 | S → D: No effect on viral replication. 1 Publication | 1 | |
Mutagenesisi | 319 | K → A: Complete loss of dsRNA binding activity; when associated with A-322. 1 Publication | 1 | |
Mutagenesisi | 322 | R → A: Complete loss of dsRNA binding activity; when associated with A-319. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000222161 | 1 – 340 | Polymerase cofactor VP35Add BLAST | 340 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 187 | Phosphoserine; by host2 Publications | 1 | |
Modified residuei | 205 | Phosphoserine; by host2 Publications | 1 | |
Modified residuei | 206 | Phosphothreonine; by host2 Publications | 1 | |
Modified residuei | 207 | Phosphothreonine; by host1 Publication | 1 | |
Modified residuei | 208 | Phosphoserine; by host2 Publications | 1 | |
Modified residuei | 210 | Phosphothreonine; by host1 Publication | 1 | |
Cross-linki | 309 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 310 | Phosphoserine; by host1 Publication | 1 | |
Modified residuei | 317 | Phosphoserine; by host2 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationPTM databases
iPTMneti | Q05127 |
Interactioni
Subunit structurei
Homodimer (By similarity). Homooligomer; via the coiled coil domain (PubMed:16095644).
Interacts with nucleoprotein NP and polymerase L; VP35 bridges L and NP and allows the formation of the polymerase complex (PubMed:25865894) (Probable).
Also interacts with VP30; this interaction is regulated by VP30 phosphorylation (PubMed:23493393).
Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3.
Interacts with host DYNLL1; this interaction stabilizes VP35 N-terminal oligomerization domain, enhances viral RNA synthesis but does not participate in suppressing the host innate immune response (PubMed:19403681, PubMed:25741013).
Interacts with host PRKRA; this interaction inhibits the interaction between DDX58 and PRKRA.
Interacts with dsRNA (PubMed:19122151, PubMed:20071589, PubMed:23870315).
Interacts with host TRIM6; this interaction plays an important role in promoting efficient viral replication (PubMed:28679761).
Interacts with host STAU1 (PubMed:30301857).
Interacts with host IRF7, PIAS1 and UBE2I/UBC9; these interactions mediate the sumoylation of IRF7 and contribute to the inhibition of IFN-type I production (PubMed:19557165).
Interacts with host DICER1; this interaction prevents TARBP2/TRBP binding to DICER1 and thus allows the virus to counteract host RNA silencing (By similarity).
Interacts with host TARBP2/TRBP and PRKRA/PACT; these interactions prevent TARBP2 and PRKRA binding to DICER1 and thus allows the virus to counteract host RNA silencing (By similarity).
By similarity1 Publication11 PublicationsBinary interactionsi
Hide detailsQ05127
Protein-protein interaction databases
DIPi | DIP-48771N |
IntActi | Q05127, 62 interactors |
Structurei
Secondary structure
3D structure databases
SMRi | Q05127 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q05127 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 215 – 340 | VP35 IIDPROSITE-ProRule annotationAdd BLAST | 126 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 49 | Chaperones the nucleoproteinBy similarityAdd BLAST | 49 | |
Regioni | 33 – 48 | Intrinsically disordered1 PublicationAdd BLAST | 16 | |
Regioni | 33 – 48 | NP binding region1 PublicationAdd BLAST | 16 | |
Regioni | 83 – 145 | HomooligomerizationBy similarityAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 96 – 116 | Sequence analysisAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 71 – 75 | Required for host DYNLL1 interaction1 Publication | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilFamily and domain databases
DisProti | DP00998 |
Gene3Di | 1.10.8.950, 1 hit 2.10.10.70, 1 hit |
InterProi | View protein in InterPro IPR002953, Filo_VP35 IPR031163, VP35_IID IPR043061, VP35_IID_b-sht IPR043060, VP35_IID_hlx |
Pfami | View protein in Pfam PF02097, Filo_VP35, 1 hit |
PIRSFi | PIRSF018326, VP35_FiloV, 1 hit |
PRINTSi | PR01240, FILOVP35 |
PROSITEi | View protein in PROSITE PS51735, VP35_IID, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTTRTKGRGH TAATTQNDRM PGPELSGWIS EQLMTGRIPV SDIFCDIENN
60 70 80 90 100
PGLCYASQMQ QTKPNPKTRN SQTQTDPICN HSFEEVVQTL ASLATVVQQQ
110 120 130 140 150
TIASESLEQR ITSLENGLKP VYDMAKTISS LNRVCAEMVA KYDLLVMTTG
160 170 180 190 200
RATATAAATE AYWAEHGQPP PGPSLYEESA IRGKIESRDE TVPQSVREAF
210 220 230 240 250
NNLNSTTSLT EENFGKPDIS AKDLRNIMYD HLPGFGTAFH QLVQVICKLG
260 270 280 290 300
KDSNSLDIIH AEFQASLAEG DSPQCALIQI TKRVPIFQDA APPVIHIRSR
310 320 330 340
GDIPRACQKS LRPVPPSPKI DRGWVCVFQL QDGKTLGLKI
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 12 | A → V in strain: Isolate mouse-adapted. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61274 Genomic RNA Translation: CAA43578.1 L11365 Genomic RNA Translation: AAB81002.1 AF086833 Genomic RNA Translation: AAD14582.1 AF272001 Genomic RNA Translation: AAG40165.1 AY142960 Genomic RNA Translation: AAN37505.1 AF499101 Genomic RNA Translation: AAM76032.1 |
RefSeqi | NP_066244.1, NC_002549.1 |
Genome annotation databases
GeneIDi | 911827 |
KEGGi | vg:911827 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61274 Genomic RNA Translation: CAA43578.1 L11365 Genomic RNA Translation: AAB81002.1 AF086833 Genomic RNA Translation: AAD14582.1 AF272001 Genomic RNA Translation: AAG40165.1 AY142960 Genomic RNA Translation: AAN37505.1 AF499101 Genomic RNA Translation: AAM76032.1 |
RefSeqi | NP_066244.1, NC_002549.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FKE | X-ray | 1.40 | A/B | 215-340 | [»] | |
3L25 | X-ray | 2.00 | A/B/D/E | 215-340 | [»] | |
3L26 | X-ray | 2.40 | A/B | 215-340 | [»] | |
3L27 | X-ray | 1.95 | A/B/C/D | 215-340 | [»] | |
3L28 | X-ray | 2.40 | A/B/C/D/E/F | 215-338 | [»] | |
3L29 | X-ray | 1.70 | A/B | 215-340 | [»] | |
4IBB | X-ray | 1.75 | A/B | 215-340 | [»] | |
4IBC | X-ray | 1.74 | A/B | 215-340 | [»] | |
4IBD | X-ray | 1.84 | A/B | 215-340 | [»] | |
4IBE | X-ray | 1.95 | A/B | 215-340 | [»] | |
4IBF | X-ray | 2.29 | A/B | 215-340 | [»] | |
4IBG | X-ray | 1.41 | A/B | 215-340 | [»] | |
4IBI | X-ray | 1.47 | A/B | 215-340 | [»] | |
4IBJ | X-ray | 1.54 | A/B | 215-340 | [»] | |
4IBK | X-ray | 1.85 | A/B | 215-340 | [»] | |
4IJE | X-ray | 1.90 | A/B/C/D | 218-340 | [»] | |
4IJF | X-ray | 2.51 | A | 218-340 | [»] | |
4YPI | X-ray | 3.71 | E/F/G/H | 20-47 | [»] | |
4ZTA | X-ray | 2.40 | A | 15-59 | [»] | |
4ZTG | X-ray | 2.80 | A | 15-59 | [»] | |
4ZTI | X-ray | 2.40 | A/B | 15-59 | [»] | |
6GBO | X-ray | 2.10 | A/B/C/D/E/F/G/H/I/J/K/L | 82-145 | [»] | |
6GBP | X-ray | 3.49 | A/B/C/D/E/F/G/H/I/J/K/L | 82-145 | [»] | |
SMRi | Q05127 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48771N |
IntActi | Q05127, 62 interactors |
PTM databases
iPTMneti | Q05127 |
Protocols and materials databases
ABCDi | Q05127, 5 sequenced antibodies |
Genome annotation databases
GeneIDi | 911827 |
KEGGi | vg:911827 |
Miscellaneous databases
EvolutionaryTracei | Q05127 |
Family and domain databases
DisProti | DP00998 |
Gene3Di | 1.10.8.950, 1 hit 2.10.10.70, 1 hit |
InterProi | View protein in InterPro IPR002953, Filo_VP35 IPR031163, VP35_IID IPR043061, VP35_IID_b-sht IPR043060, VP35_IID_hlx |
Pfami | View protein in Pfam PF02097, Filo_VP35, 1 hit |
PIRSFi | PIRSF018326, VP35_FiloV, 1 hit |
PRINTSi | PR01240, FILOVP35 |
PROSITEi | View protein in PROSITE PS51735, VP35_IID, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VP35_EBOZM | |
Accessioni | Q05127Primary (citable) accession number: Q05127 Secondary accession number(s): Q77LU7, Q8JS63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families