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Protein

Potassium voltage-gated channel subfamily A member 4

Gene

KCNA4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:1505668). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA4 forms a potassium channel that opens in response to membrane depolarization, followed by rapid spontaneous channel closure (PubMed:1505668). Likewise, a heterotetrameric channel formed by KCNA1 and KCNA4 shows rapid inactivation (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 4
Alternative name(s):
BAK4
Voltage-gated potassium channel subunit Kv1.4
Gene namesi
Name:KCNA4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 312CytoplasmicBy similarityAdd BLAST312
Transmembranei313 – 334Helical; Name=Segment S1By similarityAdd BLAST22
Topological domaini335 – 378ExtracellularBy similarityAdd BLAST44
Transmembranei379 – 400Helical; Name=Segment S2By similarityAdd BLAST22
Topological domaini401 – 411CytoplasmicBy similarityAdd BLAST11
Transmembranei412 – 432Helical; Name=Segment S3By similarityAdd BLAST21
Topological domaini433 – 447ExtracellularBy similarityAdd BLAST15
Transmembranei448 – 468Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST21
Topological domaini469 – 483CytoplasmicBy similarityAdd BLAST15
Transmembranei484 – 505Helical; Name=Segment S5By similarityAdd BLAST22
Topological domaini506 – 519ExtracellularBy similarityAdd BLAST14
Intramembranei520 – 531Helical; Name=Pore helixBy similarityAdd BLAST12
Intramembranei532 – 539By similarity8
Topological domaini540 – 546ExtracellularBy similarity7
Transmembranei547 – 575Helical; Name=Segment S6By similarityAdd BLAST29
Topological domaini576 – 660CytoplasmicBy similarityAdd BLAST85

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539801 – 660Potassium voltage-gated channel subfamily A member 4Add BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122PhosphoserineBy similarity1
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei607Phosphoserine; by PKABy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ05037
PRIDEiQ05037

Expressioni

Tissue specificityi

Detected in cerebellum, corpus striatum, hippocampus, cerebral cortex and brain stem (at protein level).1 Publication

Interactioni

Subunit structurei

Homotetramer and heterotetramer of potassium channel proteins (By similarity). Interacts with KCNAB1 and KCNAB2 (By similarity). Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity). Interacts with SIGMAR1 (By similarity). Detected in a complex with KCNA1 (PubMed:8110763). Interacts with KCNA2 (By similarity). Part of a complex containing KCNA1, KCNAB1 and LGI1 (By similarity). Interacts (via cytoplasmic N-terminal domain) with KCNRG (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027710

Structurei

3D structure databases

ProteinModelPortaliQ05037
SMRiQ05037
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni470 – 483S4-S5 linkerBy similarityAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi532 – 537Selectivity filterBy similarity6
Motifi658 – 660PDZ-bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi38 – 50Poly-AlaAdd BLAST13
Compositional biasi53 – 59Poly-Gly7
Compositional biasi62 – 65Poly-His4
Compositional biasi83 – 87Poly-Arg5
Compositional biasi131 – 137Poly-Glu7
Compositional biasi162 – 173Poly-GlyAdd BLAST12
Compositional biasi441 – 444Poly-Gln4

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.By similarity
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1545 Eukaryota
COG1226 LUCA
HOVERGENiHBG052230
InParanoidiQ05037

Family and domain databases

Gene3Di1.20.120.350, 1 hit
1.20.5.600, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR005821 Ion_trans_dom
IPR003968 K_chnl_volt-dep_Kv
IPR003972 K_chnl_volt-dep_Kv1
IPR020467 K_chnl_volt-dep_Kv1.4
IPR012897 K_chnl_volt-dep_Kv1.4_TID
IPR037065 K_chnl_volt-dep_Kv1.4_TID_sf
IPR011333 SKP1/BTB/POZ_sf
IPR003131 T1-type_BTB
IPR028325 VG_K_chnl
IPR027359 Volt_channel_dom_sf
PANTHERiPTHR11537 PTHR11537, 1 hit
PfamiView protein in Pfam
PF02214 BTB_2, 1 hit
PF00520 Ion_trans, 1 hit
PF07941 K_channel_TID, 1 hit
PRINTSiPR00169 KCHANNEL
PR01511 KV14CHANNEL
PR01491 KVCHANNEL
PR01496 SHAKERCHANEL
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit

Sequencei

Sequence statusi: Complete.

Q05037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVAMVSAES SGCNSHMPYG YAAQARARER ERLAHSRAAA AAAVAAATAA
60 70 80 90 100
VEGGGGSGGS QHHHHPSRGA CTSHDPQSGR GSRRRRRPHP EKKKVHHRQS
110 120 130 140 150
SFPHCSDLMP SGSEEKILRD LSEEDEEEDD EEDEEEEGRF YYSEEDHGEE
160 170 180 190 200
CSYTDLLAQD DGGGGGGGSG GGGYSSVRYS DCCERVVINV SGLRFETQMK
210 220 230 240 250
TLAQFPETLL GDPEKRTQYF DPLRNEYFFD RNRPSFDAIL YYYQSGGRLK
260 270 280 290 300
RPVNVPFDIF TEEVKFYQLG EEALLKFRED EGFVREEEDR ALPENEFKKQ
310 320 330 340 350
IWLLFEYPES SSPARGIAIV SVLVILISIV IFCLETLPEF RDDRDLIMAL
360 370 380 390 400
STGGHGGLLN DTSAPHPENS GHTIFNDPFF IVETVCIVWF SFEFVVRCFA
410 420 430 440 450
CPSQALFFKN IMNIIDIVSI LPYFITLGTD LAQQQGGGNG QQQQAMSFAI
460 470 480 490 500
LRIIRLVRVF RIFKLSRHSK GLQILGHTLR ASMRELGLLI FFLFIGVILF
510 520 530 540 550
SSAVYFAEAD EPTTHFQSIP DAFWWAVVTM TTVGYGDMKP ITVGGKIVGS
560 570 580 590 600
LCAIAGVLTI ALPVPVIVSN FNYFYHRETE NEEQTQLTQN AVSCPYLPSN
610 620 630 640 650
LLKKFRSSTS SSLGDKSEYL EMEEGVKESL CAKEKCQGKG DDSETDKNNV
660
SNAKAVETDV
Length:660
Mass (Da):73,513
Last modified:November 1, 1995 - v1
Checksum:iA1FAEE59677929D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57033 mRNA Translation: CAA40349.1
PIRiS24125
UniGeneiBt.309

Similar proteinsi

Entry informationi

Entry nameiKCNA4_BOVIN
AccessioniPrimary (citable) accession number: Q05037
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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