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UniProtKB - Q05031 (DFG5_YEAST)
Protein
Mannan endo-1,6-alpha-mannosidase DFG5
Gene
DFG5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for normal synthesis of the cell wall.
1 PublicationMiscellaneous
Present with 2950 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans. EC:3.2.1.101
GO - Molecular functioni
- mannan endo-1,6-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- budding cell bud growth Source: SGD
- carbohydrate catabolic process Source: InterPro
- cell wall organization Source: UniProtKB-KW
- fungal-type cell wall biogenesis Source: SGD
- pseudohyphal growth Source: SGD
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Protein family/group databases
CAZyi | GH76, Glycoside Hydrolase Family 76 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannan endo-1,6-alpha-mannosidase DFG5 (EC:3.2.1.101)Alternative name(s): Endo-alpha-1->6-D-mannanase DFG5 |
Gene namesi | Name:DFG5 Ordered Locus Names:YMR238W ORF Names:YM9959.20 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004851, DFG5 |
VEuPathDBi | FungiDB:YMR238W |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; GPI-anchor 1 Publication
Note: GPI-anchored plasma membrane protein (GPI-PMP).
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Plasma Membrane
- anchored component of plasma membrane Source: SGD
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000012127 | 27 – 437 | Mannan endo-1,6-alpha-mannosidase DFG5Add BLAST | 411 | |
PropeptideiPRO_0000012128 | 438 – 458 | Removed in mature formSequence analysisAdd BLAST | 21 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 89 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 138 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 208 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 231 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 245 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 270 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 273 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 417 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 437 | GPI-anchor amidated glycineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.2 Publications
Keywords - PTMi
Glycoprotein, GPI-anchor, LipoproteinProteomic databases
MaxQBi | Q05031 |
PaxDbi | Q05031 |
PRIDEi | Q05031 |
Interactioni
Binary interactionsi
Q05031
With | #Exp. | IntAct |
---|---|---|
ORM2 [Q06144] | 3 | EBI-27512,EBI-34916 |
Protein-protein interaction databases
BioGRIDi | 35416, 130 interactors |
DIPi | DIP-2739N |
IntActi | Q05031, 15 interactors |
MINTi | Q05031 |
STRINGi | 4932.YMR238W |
Miscellaneous databases
RNActi | Q05031, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 399 – 418 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 76 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QWHG, Eukaryota |
HOGENOMi | CLU_025694_1_2_1 |
InParanoidi | Q05031 |
OMAi | WNAGEAF |
Family and domain databases
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR005198, Glyco_hydro_76 IPR014480, Mannan-1_6-alpha_mannosidase |
PANTHERi | PTHR12145, PTHR12145, 1 hit |
Pfami | View protein in Pfam PF03663, Glyco_hydro_76, 1 hit |
PIRSFi | PIRSF016302, Man_a_manosd, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q05031-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVNISAKMI LSICFTFLSF FKATHAMDLD TTSKTSICDA TALIQGGMLD
60 70 80 90 100
YYEGTRYGGT VGMFQSPYYW WHAGEAFGGM LENWFLCEND TYQELLYDAL
110 120 130 140 150
LAQTGSNYDY IPSNQTMVEG NDDQGIWGIT VMGAVERNFT DPGDGKPGWL
160 170 180 190 200
AMVQAVFNTM YSRWDSEHCG GGLRWQIFTW NSGYNYKNTV SNACLFQIAA
210 220 230 240 250
RLGRYTGNTT YLEVAEQVFD WLVDVGYVVL NDTANVFDGA EIDTNCTDIT
260 270 280 290 300
KIEWTYNHGI VLGGLAYMYN ATNGTGEWET SLTKILNGAK SYFFKDSIMY
310 320 330 340 350
ESACQDYGTC NTDQRTFKSI FSRMLGLTSV MAPFTRDTID DLIKTSAEAA
360 370 380 390 400
AKSCNGGTDG HTCGLNWQKQ TNDGYYGLGE QMSALEVIQN LLIHDRPAPY
410 420 430 440 450
KEDNGGTSKG DANAGMNSST TNVLQNNLNI KKGDRAGAAI ITAVILSVLT
GGAVWMLF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49939 Genomic DNA Translation: CAA90209.1 BK006946 Genomic DNA Translation: DAA10138.1 |
PIRi | S57605 |
RefSeqi | NP_013965.1, NM_001182745.1 |
Genome annotation databases
EnsemblFungii | YMR238W_mRNA; YMR238W; YMR238W |
GeneIDi | 855278 |
KEGGi | sce:YMR238W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49939 Genomic DNA Translation: CAA90209.1 BK006946 Genomic DNA Translation: DAA10138.1 |
PIRi | S57605 |
RefSeqi | NP_013965.1, NM_001182745.1 |
3D structure databases
SMRi | Q05031 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35416, 130 interactors |
DIPi | DIP-2739N |
IntActi | Q05031, 15 interactors |
MINTi | Q05031 |
STRINGi | 4932.YMR238W |
Protein family/group databases
CAZyi | GH76, Glycoside Hydrolase Family 76 |
Proteomic databases
MaxQBi | Q05031 |
PaxDbi | Q05031 |
PRIDEi | Q05031 |
Genome annotation databases
EnsemblFungii | YMR238W_mRNA; YMR238W; YMR238W |
GeneIDi | 855278 |
KEGGi | sce:YMR238W |
Organism-specific databases
SGDi | S000004851, DFG5 |
VEuPathDBi | FungiDB:YMR238W |
Phylogenomic databases
eggNOGi | ENOG502QWHG, Eukaryota |
HOGENOMi | CLU_025694_1_2_1 |
InParanoidi | Q05031 |
OMAi | WNAGEAF |
Miscellaneous databases
PROi | PR:Q05031 |
RNActi | Q05031, protein |
Family and domain databases
InterProi | View protein in InterPro IPR008928, 6-hairpin_glycosidase_sf IPR005198, Glyco_hydro_76 IPR014480, Mannan-1_6-alpha_mannosidase |
PANTHERi | PTHR12145, PTHR12145, 1 hit |
Pfami | View protein in Pfam PF03663, Glyco_hydro_76, 1 hit |
PIRSFi | PIRSF016302, Man_a_manosd, 1 hit |
SUPFAMi | SSF48208, SSF48208, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DFG5_YEAST | |
Accessioni | Q05031Primary (citable) accession number: Q05031 Secondary accession number(s): D6W064 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | September 29, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families