UniProtKB - Q04957 (DPO1_BACCA)
Protein
DNA polymerase I
Gene
polA
Organism
Bacillus caldotenax
Status
Functioni
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.
Catalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA-dependent DNA replication Source: InterPro
- DNA repair Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:polA |
Organismi | Bacillus caldotenax |
Taxonomic identifieri | 1395 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Geobacillus thermoleovorans group |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101233 | 1 – 877 | DNA polymerase IAdd BLAST | 877 |
Interactioni
Subunit structurei
Single-chain monomer with multiple functions.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q04957 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q04957 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 310 | 5'-3' exonucleaseAdd BLAST | 310 | |
Domaini | 311 – 465 | 3'-5' exonucleaseAdd BLAST | 155 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 469 – 877 | PolymeraseBy similarityAdd BLAST | 409 |
Sequence similaritiesi
Belongs to the DNA polymerase type-A family.Curated
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
i Sequence
Sequence statusi: Complete.
Q04957-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKKLVLIDG SSVAYRAFFA LPLLHNDKGI HTNAVYGFTM MLNKILAEEE
60 70 80 90 100
PTHMLVAFDA GKTTFRHEAF QEYKGGRQQT PPELSEQFPL LRELLRAYRI
110 120 130 140 150
PAYELENYEA DDIIGTLAAR AEQEGFEVKV ISGDRDLTQL ASPHVTVDIT
160 170 180 190 200
KKGITDIEPY TPEAVREKYG LTPEQIVDLK GLMGDKSDNI PGVPGIGEKT
210 220 230 240 250
AVKLLRQFGT VENVLASIDE IKGEKLKETL RQHREMALLS KKLAAIRRDA
260 270 280 290 300
PVELSLDDIA YQGEDREKVV ALFKELGFQS FLEKMESPSS EEEKPLAKMA
310 320 330 340 350
FTLADRVTEE MLADKAALVV EVVEENYHDA PIVGIAVVNE HGRFFLRPET
360 370 380 390 400
ALADPQFVAW LGDETKKKSM FDSKRAAVAL KWKGIELCGV SFDLLLAAYL
410 420 430 440 450
LDPAQGVDDV AAAAKMKQYE AVRPDEAVYG KGAKRAVPDE PVLAEHLVRK
460 470 480 490 500
AAAIWALERP FLDELRRNEQ DRLLVELEQP LSSILAEMEF AGVKVDTKRL
510 520 530 540 550
EQMGEELAEQ LRTVEQRIYE LAGQEFNINS PKQLGVILFE KLQLPVLKKS
560 570 580 590 600
KTGYSTSADV LEKLAPYHEI VENILQHYRQ LGKLQSTYIE GLLKVVRPDT
610 620 630 640 650
KKVHTIFNQA LTQTGRLSST EPNLQNIPIR LEEGRKIRQA FVPSESDWLI
660 670 680 690 700
FAADYSQIEL RVLAHIAEDD NLMEAFRRDL DIHTKTAMDI FQVSEDEVTP
710 720 730 740 750
NMRRQAKAVN FGIVYGISDY GLAQNLNISR KEAAEFIERY FESFPGVKRY
760 770 780 790 800
MENIVQEAKQ KGYVTTLLHR RRYLPDITSR NFNVRSFAER MAMNTPIQGS
810 820 830 840 850
AADIIKKAMI DLNARLKEER LQARLLLQVH DELILEAPKE EMERLCRLVP
860 870
EVMEQAVTLR VPLKVDYHYG STWYDAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12982 Genomic DNA Translation: BAA02361.1 |
PIRi | JX0256 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12982 Genomic DNA Translation: BAA02361.1 |
PIRi | JX0256 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HHX | X-ray | 2.26 | A | 298-877 | [»] | |
SMRi | Q04957 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q04957 |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO1_BACCA | |
Accessioni | Q04957Primary (citable) accession number: Q04957 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | October 7, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families