UniProtKB - Q04859 (MAK_MOUSE)
Serine/threonine-protein kinase MAK
Mak
Functioni
Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR (By similarity).
By similarity1 PublicationCatalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 125 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
- transcription coactivator activity Source: UniProtKB
GO - Biological processi
- cilium assembly Source: GO_Central
- intracellular signal transduction Source: GO_Central
- intraciliary transport Source: GO_Central
- negative regulation of non-motile cilium assembly Source: MGI
- photoreceptor cell maintenance Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 3474 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase MAK (EC:2.7.11.1By similarity)Alternative name(s): Male germ cell-associated kinase Protein kinase RCK |
Gene namesi | Name:Mak Synonyms:Rck |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96913, Mak |
VEuPathDBi | HostDB:ENSMUSG00000021363 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- centrosome By similarity
- spindle By similarity
Other locations
- Midbody By similarity
- photoreceptor outer segment 1 Publication
- Photoreceptor inner segment By similarity
Note: Localizes in both the connecting cilia and the outer segment axonemes.
Cytoskeleton
- axoneme Source: MGI
- centrosome Source: UniProtKB
- mitotic spindle Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- cilium Source: GO_Central
- cytoplasm Source: GO_Central
- midbody Source: UniProtKB
- motile cilium Source: MGI
- photoreceptor connecting cilium Source: MGI
- photoreceptor inner segment Source: UniProtKB
- photoreceptor outer segment Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL2176784 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086285 | 1 – 622 | Serine/threonine-protein kinase MAKAdd BLAST | 622 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 157 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 159 | Phosphotyrosine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q04859 |
MaxQBi | Q04859 |
PaxDbi | Q04859 |
PRIDEi | Q04859 |
ProteomicsDBi | 292163 [Q04859-1] 292164 [Q04859-2] 292165 [Q04859-3] |
PTM databases
iPTMneti | Q04859 |
PhosphoSitePlusi | Q04859 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000021363, Expressed in retina and 118 other tissues |
Genevisiblei | Q04859, MM |
Interactioni
Subunit structurei
Interacts with AR and CDK20.
Found in a complex containing MAK, AR and NCOA3.
Interacts with FZR1 (via WD repeats) (By similarity).
Interacts with RP1.
By similarity1 PublicationProtein-protein interaction databases
DIPi | DIP-59494N |
IntActi | Q04859, 1 interactor |
STRINGi | 10090.ENSMUSP00000129615 |
Miscellaneous databases
RNActi | Q04859, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 284 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 281 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 301 – 371 | DisorderedSequence analysisAdd BLAST | 71 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 332 – 351 | Polar residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0661, Eukaryota |
GeneTreei | ENSGT00940000156581 |
HOGENOMi | CLU_000288_181_25_1 |
InParanoidi | Q04859 |
OMAi | INPYTWS |
OrthoDBi | 76933at2759 |
TreeFami | TF328769 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNRYTTMKQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE
60 70 80 90 100
VKSLKKLNHA NVIKLKEVIR ENDHLYFVFE YMKENLYQLM KDRNKLFPES
110 120 130 140 150
VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL
160 170 180 190 200
RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYTFRPLF
210 220 230 240 250
PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CIPINLKTLI
260 270 280 290 300
PNASSEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GSSAHHLDTK
310 320 330 340 350
QTLHKQLQPL EPKPSSSERD PKPLPNILDQ PAGQPQPKQG HQPLQTIQPP
360 370 380 390 400
QNTVTHPPPK QQGHQKPPQT MFPSIIKTIP VNSVSTLGHK GARRRWGQTV
410 420 430 440 450
FKSGDSCDDI EDDLGASHSK KPSMEACKEK KKESPFRFPD SGLPVSNHFK
460 470 480 490 500
GENRNLHASV SLKSDPNLST ASTAKQYYLK QSRYLPGVNP KNVSLVAGGK
510 520 530 540 550
DINSHSWNNQ LFPKSLGSMG ADLSFKRSNA AGNLGSYTTY NQTGYMPSFL
560 570 580 590 600
KKEVGSAGQR IQLAPLGASA SDYTWSTKTG RGQFSGRTYN PTAKNLNIVN
610 620
RTQPVPSVHG RTDWVAKYGG HR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A286YD80 | A0A286YD80_MOUSE | Serine/threonine-protein kinase MAK | Mak | 621 | Annotation score: | ||
A0A286YCF7 | A0A286YCF7_MOUSE | Serine/threonine-protein kinase MAK | Mak | 194 | Annotation score: | ||
A0A286YCX7 | A0A286YCX7_MOUSE | Serine/threonine-protein kinase MAK | Mak | 168 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 128 | P → H in BAC26662 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 235 | N → D in BAC26662 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 386 | T → A in CAA47392 (PubMed:8359591).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042472 | 94 – 124 | Missing in isoform 3. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_042473 | 531 – 571 | Missing in isoform 3. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_042474 | 531 | A → EDSIIKPIENLSCTGKSAEQ LEDPQ in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66983 mRNA Translation: CAA47392.1 AK029894 mRNA Translation: BAC26662.1 AC133496 Genomic DNA No translation available. |
CCDSi | CCDS26471.1 [Q04859-1] CCDS49244.1 [Q04859-3] CCDS49245.1 [Q04859-2] |
PIRi | I48733 |
RefSeqi | NP_001139274.1, NM_001145802.1 [Q04859-3] NP_001139275.1, NM_001145803.1 [Q04859-2] NP_032573.2, NM_008547.2 [Q04859-1] XP_006516934.1, XM_006516871.2 [Q04859-1] |
Genome annotation databases
Ensembli | ENSMUST00000021792; ENSMUSP00000021792; ENSMUSG00000021363 [Q04859-1] ENSMUST00000070193; ENSMUSP00000064750; ENSMUSG00000021363 [Q04859-3] ENSMUST00000165087; ENSMUSP00000129615; ENSMUSG00000021363 [Q04859-2] ENSMUST00000225084; ENSMUSP00000152946; ENSMUSG00000021363 [Q04859-2] |
GeneIDi | 17152 |
KEGGi | mmu:17152 |
UCSCi | uc007qev.2, mouse [Q04859-1] uc007qew.2, mouse [Q04859-2] uc007qex.2, mouse [Q04859-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66983 mRNA Translation: CAA47392.1 AK029894 mRNA Translation: BAC26662.1 AC133496 Genomic DNA No translation available. |
CCDSi | CCDS26471.1 [Q04859-1] CCDS49244.1 [Q04859-3] CCDS49245.1 [Q04859-2] |
PIRi | I48733 |
RefSeqi | NP_001139274.1, NM_001145802.1 [Q04859-3] NP_001139275.1, NM_001145803.1 [Q04859-2] NP_032573.2, NM_008547.2 [Q04859-1] XP_006516934.1, XM_006516871.2 [Q04859-1] |
3D structure databases
AlphaFoldDBi | Q04859 |
SMRi | Q04859 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-59494N |
IntActi | Q04859, 1 interactor |
STRINGi | 10090.ENSMUSP00000129615 |
Chemistry databases
ChEMBLi | CHEMBL2176784 |
PTM databases
iPTMneti | Q04859 |
PhosphoSitePlusi | Q04859 |
Proteomic databases
jPOSTi | Q04859 |
MaxQBi | Q04859 |
PaxDbi | Q04859 |
PRIDEi | Q04859 |
ProteomicsDBi | 292163 [Q04859-1] 292164 [Q04859-2] 292165 [Q04859-3] |
Protocols and materials databases
Antibodypediai | 24855, 432 antibodies from 30 providers |
DNASUi | 17152 |
Genome annotation databases
Ensembli | ENSMUST00000021792; ENSMUSP00000021792; ENSMUSG00000021363 [Q04859-1] ENSMUST00000070193; ENSMUSP00000064750; ENSMUSG00000021363 [Q04859-3] ENSMUST00000165087; ENSMUSP00000129615; ENSMUSG00000021363 [Q04859-2] ENSMUST00000225084; ENSMUSP00000152946; ENSMUSG00000021363 [Q04859-2] |
GeneIDi | 17152 |
KEGGi | mmu:17152 |
UCSCi | uc007qev.2, mouse [Q04859-1] uc007qew.2, mouse [Q04859-2] uc007qex.2, mouse [Q04859-3] |
Organism-specific databases
CTDi | 4117 |
MGIi | MGI:96913, Mak |
VEuPathDBi | HostDB:ENSMUSG00000021363 |
Phylogenomic databases
eggNOGi | KOG0661, Eukaryota |
GeneTreei | ENSGT00940000156581 |
HOGENOMi | CLU_000288_181_25_1 |
InParanoidi | Q04859 |
OMAi | INPYTWS |
OrthoDBi | 76933at2759 |
TreeFami | TF328769 |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 17152, 0 hits in 74 CRISPR screens |
ChiTaRSi | Ddx6, mouse |
PROi | PR:Q04859 |
RNActi | Q04859, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021363, Expressed in retina and 118 other tissues |
Genevisiblei | Q04859, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAK_MOUSE | |
Accessioni | Q04859Primary (citable) accession number: Q04859 Secondary accession number(s): E9Q4B2 Q8CDL5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families