Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-3-methyladenine glycosylase

Gene

Mpg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Enzyme regulationi

Binding to SSBP1 in mitochondria inhibits glycosylase activity in the context of a single-stranded DNA (ssDNA), but not a double-stranded DNA (dsDNA) substrates.By similarity

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: MGI

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-110331 Cleavage of the damaged purine
R-MMU-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase (EC:3.2.2.21)
Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase
Gene namesi
Name:Mpg
Synonyms:Mid1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97073 Mpg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001000661 – 333DNA-3-methyladenine glycosylaseAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei98PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ04841
MaxQBiQ04841
PaxDbiQ04841
PeptideAtlasiQ04841
PRIDEiQ04841

PTM databases

iPTMnetiQ04841
PhosphoSitePlusiQ04841

Expressioni

Gene expression databases

BgeeiENSMUSG00000020287
CleanExiMM_MID1
MM_MPG
ExpressionAtlasiQ04841 baseline and differential
GenevisibleiQ04841 MM

Interactioni

Subunit structurei

Binds MBD1. Binds SSBP1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020528

Structurei

3D structure databases

ProteinModelPortaliQ04841
SMRiQ04841
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA glycosylase MPG family.Curated

Phylogenomic databases

eggNOGiKOG4486 Eukaryota
COG2094 LUCA
GeneTreeiENSGT00390000009825
HOGENOMiHOG000224224
HOVERGENiHBG000019
InParanoidiQ04841
KOiK03652
OMAiTAYVYII
OrthoDBiEOG091G0NE4
TreeFamiTF331768

Family and domain databases

CDDicd00540 AAG, 1 hit
Gene3Di3.10.300.10, 1 hit
HAMAPiMF_00527 3MGH, 1 hit
InterProiView protein in InterPro
IPR011034 Formyl_transferase-like_C_sf
IPR003180 MPG
IPR036995 MPG_sf
PANTHERiPTHR10429 PTHR10429, 1 hit
PfamiView protein in Pfam
PF02245 Pur_DNA_glyco, 1 hit
SUPFAMiSSF50486 SSF50486, 1 hit
TIGRFAMsiTIGR00567 3mg, 1 hit

Sequencei

Sequence statusi: Complete.

Q04841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPARGGSARP GRGALKPVSV TLLPDTEQPP FLGRARRPGN ARAGSLVTGY
60 70 80 90 100
HEVGQMPAPL SRKIGQKKQR LADSEQQQTP KERLLSTPGL RRSIYFSSPE
110 120 130 140 150
DHSGRLGPEF FDQPAVTLAR AFLGQVLVRR LADGTELRGR IVETEAYLGP
160 170 180 190 200
EDEAAHSRGG RQTPRNRGMF MKPGTLYVYL IYGMYFCLNV SSQGAGACVL
210 220 230 240 250
LRALEPLEGL ETMRQLRNSL RKSTVGRSLK DRELCSGPSK LCQALAIDKS
260 270 280 290 300
FDQRDLAQDD AVWLEHGPLE SSSPAVVVAA ARIGIGHAGE WTQKPLRFYV
310 320 330
QGSPWVSVVD RVAEQMDQPQ QTACSEGLLI VQK
Length:333
Mass (Da):36,472
Last modified:July 27, 2011 - v3
Checksum:i195E96174BFA0576
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14A → S in CAA52615 (PubMed:7832991).Curated1
Sequence conflicti14A → S in AAA19487 (PubMed:8435858).Curated1
Sequence conflicti14A → S in AAH14754 (PubMed:15489334).Curated1
Sequence conflicti319 – 324PQQTAC → LANSLL in AAA02577 (PubMed:8318735).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74509
, X75038, X75039, X75040 Genomic DNA Translation: CAA52615.1
U10420 mRNA Translation: AAA19487.1
S81162 Genomic DNA No translation available.
S81120 Genomic DNA No translation available.
S81133 Genomic DNA No translation available.
S81134 Genomic DNA No translation available.
AL929446 Genomic DNA Translation: CAM16547.1
BC014754 mRNA Translation: AAH14754.1
M99625 mRNA Translation: AAA02577.1
CCDSiCCDS24520.1
PIRiA49003
I62129
RefSeqiNP_034952.2, NM_010822.3
UniGeneiMm.263161
Mm.491092

Genome annotation databases

EnsembliENSMUST00000020528; ENSMUSP00000020528; ENSMUSG00000020287
GeneIDi268395
KEGGimmu:268395
UCSCiuc007ijd.2 mouse

Similar proteinsi

Entry informationi

Entry namei3MG_MOUSE
AccessioniPrimary (citable) accession number: Q04841
Secondary accession number(s): A2AVE7, Q64182
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: November 22, 2017
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health