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Protein

Methylosome subunit pICln

Gene

Clns1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also indirectly participate in cellular volume control by activation of a swelling-induced chloride conductance pathway (By similarity).By similarity

Caution

Was originally thought to be a chloride channel.Curated

GO - Biological processi

  • cell volume homeostasis Source: RGD
  • chloride transport Source: RGD
  • spliceosomal snRNP assembly Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome subunit pICln
Alternative name(s):
Chloride channel, nucleotide sensitive 1A
Chloride conductance regulatory protein ICln
Short name:
I(Cln)
Gene namesi
Name:Clns1a
Synonyms:Icln, Rcl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61884 Clns1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001851572 – 236Methylosome subunit pIClnAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei95PhosphoserineCombined sources1
Modified residuei143PhosphoserineBy similarity1
Modified residuei192PhosphoserineBy similarity1
Modified residuei194PhosphoserineBy similarity1
Modified residuei197PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Modified residuei222PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ04753
PRIDEiQ04753

PTM databases

iPTMnetiQ04753
PhosphoSitePlusiQ04753

Expressioni

Tissue specificityi

Expressed in most tissues.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least PRMT5/SKB1, WDR77/MEP50 and CLNS1A/pICln. May mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts with LSM10 and LSM11 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017702

Structurei

3D structure databases

ProteinModelPortaliQ04753
SMRiQ04753
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3238 Eukaryota
ENOG4111R94 LUCA
HOVERGENiHBG003108
InParanoidiQ04753
KOiK05019

Family and domain databases

InterProiView protein in InterPro
IPR003521 ICln
PRINTSiPR01348 ICLNCHANNEL

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q04753-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFLKSFPPP GSADGLRLQQ PDTEAVLNGK GLGTGTLYIA ESRLSWLDGS
60 70 80 90 100
GLGFSLEYPT ISLHAVSRDP NAYPQEHLYV MVNARFGEES KEPFSDEDED
110 120 130 140 150
DNDDVEPISE FRFVPSDKSA LEAMFTAMCE CQALHPDPED EDSDDYDGEE
160 170 180 190 200
YDVEAHEQGQ GDIPTFYTYE EGLSHLTAEG QATLERLEGM LSQSVSSQYN
210 220 230
MAGVRTEDSV RTYEDGMEVE TTPTVAGQFE DADVDH
Length:236
Mass (Da):26,092
Last modified:June 1, 1994 - v1
Checksum:i7430F2B96B5212A7
GO

Sequence cautioni

The sequence AAC37642 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17698 mRNA Translation: BAA04561.1
D13985 mRNA Translation: BAA03092.1
L26450 mRNA Translation: AAC37642.1 Different initiation.
PIRiB53014
RefSeqiNP_113907.1, NM_031719.1
XP_006229854.1, XM_006229792.2
UniGeneiRn.4089

Genome annotation databases

GeneIDi65160
KEGGirno:65160
UCSCiRGD:61884 rat

Similar proteinsi

Entry informationi

Entry nameiICLN_RAT
AccessioniPrimary (citable) accession number: Q04753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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