UniProtKB - Q04736 (YES_MOUSE)
Protein
Tyrosine-protein kinase Yes
Gene
Yes1
Organism
Mus musculus (Mouse)
Status
Functioni
Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).By similarity
Catalytic activityi
- EC:2.7.10.2PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 303 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 394 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 281 – 289 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- enzyme binding Source: MGI
- epidermal growth factor receptor binding Source: MGI
- ion channel binding Source: MGI
- non-membrane spanning protein tyrosine kinase activity Source: GO_Central
- phosphotyrosine residue binding Source: MGI
- protein tyrosine kinase activity Source: MGI
- signaling receptor binding Source: GO_Central
- transmembrane receptor protein tyrosine kinase activity Source: RHEA
GO - Biological processi
- cell differentiation Source: GO_Central
- cellular response to platelet-derived growth factor stimulus Source: MGI
- cellular response to retinoic acid Source: MGI
- cellular response to transforming growth factor beta stimulus Source: MGI
- innate immune response Source: GO_Central
- positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein autophosphorylation Source: MGI
- regulation of glucose transmembrane transport Source: MGI
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 3474 |
Reactomei | R-MMU-1227986, Signaling by ERBB2 R-MMU-1433557, Signaling by SCF-KIT R-MMU-1433559, Regulation of KIT signaling R-MMU-2029481, FCGR activation R-MMU-210990, PECAM1 interactions R-MMU-389356, CD28 co-stimulation R-MMU-389513, CTLA4 inhibitory signaling R-MMU-3928662, EPHB-mediated forward signaling R-MMU-3928663, EPHA-mediated growth cone collapse R-MMU-3928665, EPH-ephrin mediated repulsion of cells R-MMU-912631, Regulation of signaling by CBL |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine-protein kinase Yes (EC:2.7.10.2)Alternative name(s): Proto-oncogene c-Yes p61-Yes |
Gene namesi | Name:Yes1 Synonyms:Yes |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:99147, Yes1 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Plasma membrane
- Cell membrane By similarity
Note: Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway.By similarity
Cytoskeleton
- actin filament Source: MGI
- microtubule organizing center Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
- plasma membrane Source: MGI
Other locations
- glutamatergic synapse Source: MGI
- postsynaptic specialization, intracellular component Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Disruption phenotypei
Mice are viable, fertile, and display no apparent phenotypes. This lack of phenotype may be attributable to compensatory roles of the other SRC-family members.1 Publication
Keywords - Diseasei
Proto-oncogenePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000088182 | 2 – 541 | Tyrosine-protein kinase YesAdd BLAST | 540 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Lipidationi | 3 | S-palmitoyl cysteine; in membrane formBy similarity | 1 | |
Modified residuei | 32 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 334 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 343 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 424 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 535 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-424 maintains enzyme activity by blocking CSK-mediated inhibition (By similarity).By similarity
Palmitoylation at Cys-3 promotes membrane localization.By similarity
Keywords - PTMi
Lipoprotein, Myristate, Palmitate, PhosphoproteinProteomic databases
jPOSTi | Q04736 |
PaxDbi | Q04736 |
PeptideAtlasi | Q04736 |
PRIDEi | Q04736 |
ProteomicsDBi | 299631 |
PTM databases
iPTMneti | Q04736 |
PhosphoSitePlusi | Q04736 |
SwissPalmi | Q04736 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000014932, Expressed in cumulus cell and 286 other tissues |
Genevisiblei | Q04736, MM |
Interactioni
Subunit structurei
Interacts with YAP1.
Interacts with FASLG.
Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1 (By similarity).
Interacts with CSF1R.
Interacts with IL6ST/gp130 (By similarity).
By similarity1 PublicationGO - Molecular functioni
- enzyme binding Source: MGI
- epidermal growth factor receptor binding Source: MGI
- ion channel binding Source: MGI
- phosphotyrosine residue binding Source: MGI
- signaling receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 204615, 7 interactors |
IntActi | Q04736, 4 interactors |
MINTi | Q04736 |
STRINGi | 10090.ENSMUSP00000072154 |
Miscellaneous databases
RNActi | Q04736, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q04736 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q04736 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 89 – 150 | SH3PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 156 – 253 | SH2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 275 – 528 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Keywords - Domaini
SH2 domain, SH3 domainPhylogenomic databases
eggNOGi | KOG0197, Eukaryota |
GeneTreei | ENSGT00940000154920 |
InParanoidi | Q04736 |
OMAi | SVNFNSH |
OrthoDBi | 539311at2759 |
PhylomeDBi | Q04736 |
TreeFami | TF351634 |
Family and domain databases
CDDi | cd12007, SH3_Yes, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR035751, Yes_SH3 |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00017, SH2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF55550, SSF55550, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50001, SH2, 1 hit PS50002, SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q04736-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGCIKSKENK SPAIKYTPEN LTEPVSPSAS HYGVEHATVA PTSSTKGASV
60 70 80 90 100
NFNSLSMTPF GGSSGVTPFG GASSSFSVVS SSYPTGLTGG VTIFVALYDY
110 120 130 140 150
EARTTEDLSF KKGERFQIIN NTEGDWWEAR SIATGKSGYI PSNYVVPADS
160 170 180 190 200
IQAEEWYFGK MGRKDAERLL LNPGNQRGIF LVRESETTKG AYSLSIRDWD
210 220 230 240 250
EVRGDNVKHY KIRKLDNGGY YITTRAQFDT LQKLVKHYTE HADGLCHKLT
260 270 280 290 300
TVCPTVKPQT QGLAKDAWEI PRESLRLEVK LGQGCFGEVW MGTWNGTTKV
310 320 330 340 350
AIKTLKPGTM MPEAFLQEAQ IMKKLRHDKL VPLYAVVSEE PIYIVTEFMS
360 370 380 390 400
KGSLLDFLKE GDGKYLKLPQ LVDMAAQIAD GMAYIERMNY IHRDLRAANI
410 420 430 440 450
LVGENLICKI ADFGLARLIE DNEYTARQGA KFPIKWTAPE AALYGRFTIK
460 470 480 490 500
SDVWSFGILQ TELVTKGRVP YPGMVNREVL EQVERGYRMP CPQGCPESLH
510 520 530 540
ELMNLCWKKD PDERPTFEYI QSFLEDYFTA TEPQYQPGEN L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YUV2 | A0A0J9YUV2_MOUSE | Tyrosine-protein kinase Yes | Yes1 | 198 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67677 mRNA Translation: CAA47909.1 BC010594 mRNA Translation: AAH10594.1 L25762 mRNA Translation: AAA40020.1 |
CCDSi | CCDS39061.1 |
PIRi | I48318, S31645 |
RefSeqi | NP_001192061.1, NM_001205132.1 NP_001192062.1, NM_001205133.1 NP_033561.1, NM_009535.3 |
Genome annotation databases
Ensembli | ENSMUST00000072311; ENSMUSP00000072154; ENSMUSG00000014932 ENSMUST00000168707; ENSMUSP00000132161; ENSMUSG00000014932 ENSMUST00000202543; ENSMUSP00000144001; ENSMUSG00000014932 |
GeneIDi | 22612 |
KEGGi | mmu:22612 |
UCSCi | uc008wzs.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67677 mRNA Translation: CAA47909.1 BC010594 mRNA Translation: AAH10594.1 L25762 mRNA Translation: AAA40020.1 |
CCDSi | CCDS39061.1 |
PIRi | I48318, S31645 |
RefSeqi | NP_001192061.1, NM_001205132.1 NP_001192062.1, NM_001205133.1 NP_033561.1, NM_009535.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YT6 | NMR | - | A | 71-167 | [»] | |
5MTJ | X-ray | 1.95 | A | 147-260 | [»] | |
SMRi | Q04736 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204615, 7 interactors |
IntActi | Q04736, 4 interactors |
MINTi | Q04736 |
STRINGi | 10090.ENSMUSP00000072154 |
PTM databases
iPTMneti | Q04736 |
PhosphoSitePlusi | Q04736 |
SwissPalmi | Q04736 |
Proteomic databases
jPOSTi | Q04736 |
PaxDbi | Q04736 |
PeptideAtlasi | Q04736 |
PRIDEi | Q04736 |
ProteomicsDBi | 299631 |
Protocols and materials databases
Antibodypediai | 3819, 485 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000072311; ENSMUSP00000072154; ENSMUSG00000014932 ENSMUST00000168707; ENSMUSP00000132161; ENSMUSG00000014932 ENSMUST00000202543; ENSMUSP00000144001; ENSMUSG00000014932 |
GeneIDi | 22612 |
KEGGi | mmu:22612 |
UCSCi | uc008wzs.2, mouse |
Organism-specific databases
CTDi | 7525 |
MGIi | MGI:99147, Yes1 |
Phylogenomic databases
eggNOGi | KOG0197, Eukaryota |
GeneTreei | ENSGT00940000154920 |
InParanoidi | Q04736 |
OMAi | SVNFNSH |
OrthoDBi | 539311at2759 |
PhylomeDBi | Q04736 |
TreeFami | TF351634 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2, 3474 |
Reactomei | R-MMU-1227986, Signaling by ERBB2 R-MMU-1433557, Signaling by SCF-KIT R-MMU-1433559, Regulation of KIT signaling R-MMU-2029481, FCGR activation R-MMU-210990, PECAM1 interactions R-MMU-389356, CD28 co-stimulation R-MMU-389513, CTLA4 inhibitory signaling R-MMU-3928662, EPHB-mediated forward signaling R-MMU-3928663, EPHA-mediated growth cone collapse R-MMU-3928665, EPH-ephrin mediated repulsion of cells R-MMU-912631, Regulation of signaling by CBL |
Miscellaneous databases
BioGRID-ORCSi | 22612, 1 hit in 53 CRISPR screens |
ChiTaRSi | Yes1, mouse |
EvolutionaryTracei | Q04736 |
PROi | PR:Q04736 |
RNActi | Q04736, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014932, Expressed in cumulus cell and 286 other tissues |
Genevisiblei | Q04736, MM |
Family and domain databases
CDDi | cd12007, SH3_Yes, 1 hit |
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR000980, SH2 IPR036860, SH2_dom_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR035751, Yes_SH3 |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF00017, SH2, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00401, SH2DOMAIN PR00452, SH3DOMAIN PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00326, SH3, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF55550, SSF55550, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS50001, SH2, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YES_MOUSE | |
Accessioni | Q04736Primary (citable) accession number: Q04736 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 212 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families