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Protein

Transducin-like enhancer protein 1

Gene

TLE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • repressing transcription factor binding Source: GO_Central
  • transcription corepressor activity Source: BHF-UCL
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-HSA-3769402 Deactivation of the beta-catenin transactivating complex
R-HSA-4641265 Repression of WNT target genes
SignaLinkiQ04724
SIGNORiQ04724

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 1
Alternative name(s):
E(Sp1) homolog
Enhancer of split groucho-like protein 1
Short name:
ESG1
Gene namesi
Name:TLE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000196781.13
HGNCiHGNC:11837 TLE1
MIMi600189 gene
neXtProtiNX_Q04724

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi486V → S: Abolishes HESX1 binding. 1 Publication1
Mutagenesisi532Y → H: Abolishes HESX1 binding. 1 Publication1
Mutagenesisi702L → S: Abolishes HESX1 binding. 1 Publication1
Mutagenesisi715S → P: Abolishes HESX1 binding. 1 Publication1

Organism-specific databases

DisGeNETi7088
OpenTargetsiENSG00000196781
PharmGKBiPA36539

Polymorphism and mutation databases

BioMutaiTLE1
DMDMi29840816

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512761 – 770Transducin-like enhancer protein 1Add BLAST770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei239PhosphoserineCombined sources1
Modified residuei259Phosphoserine; by CDK1Sequence analysis1
Modified residuei263Phosphoserine; by CDK1Sequence analysis1
Modified residuei267Phosphoserine; by CDK1Sequence analysis1
Modified residuei286PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
Ubiquitinated by XIAP/BIRC4.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ04724
MaxQBiQ04724
PaxDbiQ04724
PeptideAtlasiQ04724
PRIDEiQ04724
ProteomicsDBi58266

PTM databases

iPTMnetiQ04724
PhosphoSitePlusiQ04724

Expressioni

Tissue specificityi

In all tissues examined, mostly in brain, liver and muscle.

Gene expression databases

BgeeiENSG00000196781 Expressed in 236 organ(s), highest expression level in endometrium
CleanExiHS_TLE1
ExpressionAtlasiQ04724 baseline and differential
GenevisibleiQ04724 HS

Organism-specific databases

HPAiCAB011482
HPA071396

Interactioni

Subunit structurei

Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif). Interacts with SIX3. Interacts with EFNB1.12 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112943, 125 interactors
CORUMiQ04724
DIPiDIP-36665N
IntActiQ04724, 71 interactors
MINTiQ04724
STRINGi9606.ENSP00000365682

Structurei

Secondary structure

1770
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ04724
SMRiQ04724
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04724

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati470 – 501WD 1Add BLAST32
Repeati528 – 558WD 2Add BLAST31
Repeati572 – 602WD 3Add BLAST31
Repeati614 – 644WD 4Add BLAST31
Repeati696 – 726WD 5Add BLAST31
Repeati737 – 767WD 6Add BLAST31

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 131Q domain1 PublicationAdd BLAST131
Regioni132 – 199GP domain1 PublicationAdd BLAST68
Regioni200 – 268CcN domain1 PublicationAdd BLAST69
Regioni269 – 450SP domain1 PublicationAdd BLAST182

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 228Nuclear localization signalSequence analysis4

Domaini

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639 Eukaryota
ENOG410XPX3 LUCA
GeneTreeiENSGT00550000074465
HOGENOMiHOG000293211
HOVERGENiHBG004689
InParanoidiQ04724
OMAiRRNGPEY
OrthoDBiEOG091G181Y
PhylomeDBiQ04724
TreeFamiTF314167

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR005617 Groucho/TLE_N
IPR009146 Groucho_enhance
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR10814 PTHR10814, 1 hit
PfamiView protein in Pfam
PF03920 TLE_N, 1 hit
PF00400 WD40, 2 hits
PRINTSiPR01850 GROUCHOFAMLY
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q04724-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFPQSRHPTP HQAAGQPFKF TIPESLDRIK EEFQFLQAQY HSLKLECEKL
60 70 80 90 100
ASEKTEMQRH YVMYYEMSYG LNIEMHKQTE IAKRLNTICA QVIPFLSQEH
110 120 130 140 150
QQQVAQAVER AKQVTMAELN AIIGQQQLQA QHLSHGHGPP VPLTPHPSGL
160 170 180 190 200
QPPGIPPLGG SAGLLALSSA LSGQSHLAIK DDKKHHDAEH HRDREPGTSN
210 220 230 240 250
SLLVPDSLRG TDKRRNGPEF SNDIKKRKVD DKDSSHYDSD GDKSDDNLVV
260 270 280 290 300
DVSNEDPSSP RASPAHSPRE NGIDKNRLLK KDASSSPAST ASSASSTSLK
310 320 330 340 350
SKEMSLHEKA STPVLKSSTP TPRSDMPTPG TSATPGLRPG LGKPPAIDPL
360 370 380 390 400
VNQAAAGLRT PLAVPGPYPA PFGMVPHAGM NGELTSPGAA YASLHNMSPQ
410 420 430 440 450
MSAAAAAAAV VAYGRSPMVG FDPPPHMRVP TIPPNLAGIP GGKPAYSFHV
460 470 480 490 500
TADGQMQPVP FPPDALIGPG IPRHARQINT LNHGEVVCAV TISNPTRHVY
510 520 530 540 550
TGGKGCVKVW DISHPGNKSP VSQLDCLNRD NYIRSCKLLP DGCTLIVGGE
560 570 580 590 600
ASTLSIWDLA APTPRIKAEL TSSAPACYAL AISPDSKVCF SCCSDGNIAV
610 620 630 640 650
WDLHNQTLVR QFQGHTDGAS CIDISNDGTK LWTGGLDNTV RSWDLREGRQ
660 670 680 690 700
LQQHDFTSQI FSLGYCPTGE WLAVGMESSN VEVLHVNKPD KYQLHLHESC
710 720 730 740 750
VLSLKFAYCG KWFVSTGKDN LLNAWRTPYG ASIFQSKESS SVLSCDISVD
760 770
DKYIVTGSGD KKATVYEVIY
Length:770
Mass (Da):83,201
Last modified:March 25, 2003 - v2
Checksum:i695FD1A37410EFE5
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T3G3Q5T3G3_HUMAN
Transducin-like enhancer protein 1
TLE1
242Annotation score:
F6T2C8F6T2C8_HUMAN
Transducin-like enhancer protein 1
TLE1
127Annotation score:
Q5T3G2Q5T3G2_HUMAN
Transducin-like enhancer protein 1
TLE1
144Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti407 – 412AAAVVA → RGRRGR in AAA61192 (PubMed:1303260).Curated6
Sequence conflicti464 – 465DA → TP in AAA61192 (PubMed:1303260).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99435 mRNA Translation: AAA61192.1
AK290860 mRNA Translation: BAF83549.1
AL365190 Genomic DNA No translation available.
AL353682 Genomic DNA No translation available.
CH471089 Genomic DNA Translation: EAW62636.1
BC010100 mRNA Translation: AAH10100.1
BC015747 mRNA Translation: AAH15747.1
CCDSiCCDS6661.1
PIRiB56695
RefSeqiNP_001290032.1, NM_001303103.1
NP_001290033.1, NM_001303104.1
NP_005068.2, NM_005077.4
UniGeneiHs.197320
Hs.689805

Genome annotation databases

EnsembliENST00000376499; ENSP00000365682; ENSG00000196781
GeneIDi7088
KEGGihsa:7088
UCSCiuc004aly.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99435 mRNA Translation: AAA61192.1
AK290860 mRNA Translation: BAF83549.1
AL365190 Genomic DNA No translation available.
AL353682 Genomic DNA No translation available.
CH471089 Genomic DNA Translation: EAW62636.1
BC010100 mRNA Translation: AAH10100.1
BC015747 mRNA Translation: AAH15747.1
CCDSiCCDS6661.1
PIRiB56695
RefSeqiNP_001290032.1, NM_001303103.1
NP_001290033.1, NM_001303104.1
NP_005068.2, NM_005077.4
UniGeneiHs.197320
Hs.689805

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GXRX-ray1.65A/B443-770[»]
2CE8X-ray2.03A/B/C/D443-770[»]
2CE9X-ray2.12A/B/C/D443-770[»]
4OM2X-ray4.00A/B/C/D1-156[»]
4OM3X-ray2.86A/B/C/D15-156[»]
5MWJX-ray2.04A/B443-770[»]
ProteinModelPortaliQ04724
SMRiQ04724
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112943, 125 interactors
CORUMiQ04724
DIPiDIP-36665N
IntActiQ04724, 71 interactors
MINTiQ04724
STRINGi9606.ENSP00000365682

PTM databases

iPTMnetiQ04724
PhosphoSitePlusiQ04724

Polymorphism and mutation databases

BioMutaiTLE1
DMDMi29840816

Proteomic databases

EPDiQ04724
MaxQBiQ04724
PaxDbiQ04724
PeptideAtlasiQ04724
PRIDEiQ04724
ProteomicsDBi58266

Protocols and materials databases

DNASUi7088
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376499; ENSP00000365682; ENSG00000196781
GeneIDi7088
KEGGihsa:7088
UCSCiuc004aly.4 human

Organism-specific databases

CTDi7088
DisGeNETi7088
EuPathDBiHostDB:ENSG00000196781.13
GeneCardsiTLE1
HGNCiHGNC:11837 TLE1
HPAiCAB011482
HPA071396
MIMi600189 gene
neXtProtiNX_Q04724
OpenTargetsiENSG00000196781
PharmGKBiPA36539
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0639 Eukaryota
ENOG410XPX3 LUCA
GeneTreeiENSGT00550000074465
HOGENOMiHOG000293211
HOVERGENiHBG004689
InParanoidiQ04724
OMAiRRNGPEY
OrthoDBiEOG091G181Y
PhylomeDBiQ04724
TreeFamiTF314167

Enzyme and pathway databases

ReactomeiR-HSA-201722 Formation of the beta-catenin:TCF transactivating complex
R-HSA-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-HSA-3769402 Deactivation of the beta-catenin transactivating complex
R-HSA-4641265 Repression of WNT target genes
SignaLinkiQ04724
SIGNORiQ04724

Miscellaneous databases

ChiTaRSiTLE1 human
EvolutionaryTraceiQ04724
GeneWikiiTLE1
GenomeRNAii7088
PROiPR:Q04724
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196781 Expressed in 236 organ(s), highest expression level in endometrium
CleanExiHS_TLE1
ExpressionAtlasiQ04724 baseline and differential
GenevisibleiQ04724 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR005617 Groucho/TLE_N
IPR009146 Groucho_enhance
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR10814 PTHR10814, 1 hit
PfamiView protein in Pfam
PF03920 TLE_N, 1 hit
PF00400 WD40, 2 hits
PRINTSiPR01850 GROUCHOFAMLY
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTLE1_HUMAN
AccessioniPrimary (citable) accession number: Q04724
Secondary accession number(s): A8K495, Q5T3G4, Q969V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 25, 2003
Last modified: November 7, 2018
This is version 194 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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