UniProtKB - Q04692 (SMRCD_MOUSE)
Protein
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1
Gene
Smarcad1
Organism
Mus musculus (Mouse)
Status
Functioni
DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity).By similarity
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 516 – 524 | ATPPROSITE-ProRule annotation | 9 | |
Nucleotide bindingi | 892 – 899 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: GO_Central
- DNA binding Source: MGI
- DNA-dependent ATPase activity Source: GO_Central
- DNA helicase activity Source: UniProtKB-EC
- ubiquitin binding Source: InterPro
GO - Biological processi
- ATP-dependent chromatin remodeling Source: UniProtKB
- chromosome separation Source: UniProtKB
- DNA double-strand break processing Source: UniProtKB
- histone H3 deacetylation Source: UniProtKB
- histone H4 deacetylation Source: UniProtKB
- regulation of DNA recombination Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (EC:3.6.4.12)Alternative name(s): ATP-dependent helicase SMARCAD1 Enhancer trap locus homolog 1 Short name: Etl-1 |
Gene namesi | Name:Smarcad1 Synonyms:Etl1, Kiaa1122 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95453, Smarcad1 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Other locations
- Chromosome By similarity
Note: Colocalizes with PCNA at replication forks during S phase. Recruited to double-strand breaks (DSBs) sites of DNA damage (By similarity).By similarity
Nucleus
- nuclear replication fork Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- heterochromatin Source: UniProtKB
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Disruption phenotypei
Deficient mice have reduced viability and show growth retardation, skeletal dysplasia and impaired fertility.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074357 | 1 – 1021 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1Add BLAST | 1021 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 54 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 57 | PhosphoserineBy similarity | 1 | |
Cross-linki | 77 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 79 | PhosphoserineBy similarity | 1 | |
Modified residuei | 124 | PhosphoserineCombined sources | 1 | |
Modified residuei | 127 | PhosphoserineCombined sources | 1 | |
Modified residuei | 132 | PhosphoserineBy similarity | 1 | |
Modified residuei | 144 | PhosphoserineCombined sources | 1 | |
Modified residuei | 145 | PhosphoserineCombined sources | 1 | |
Modified residuei | 151 | PhosphoserineCombined sources | 1 | |
Modified residuei | 210 | PhosphoserineBy similarity | 1 | |
Modified residuei | 213 | PhosphoserineBy similarity | 1 | |
Modified residuei | 235 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphoserineBy similarity | 1 | |
Modified residuei | 298 | PhosphoserineBy similarity | 1 | |
Cross-linki | 330 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 466 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 719 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 991 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q04692 |
jPOSTi | Q04692 |
MaxQBi | Q04692 |
PaxDbi | Q04692 |
PeptideAtlasi | Q04692 |
PRIDEi | Q04692 |
PTM databases
iPTMneti | Q04692 |
PhosphoSitePlusi | Q04692 |
Expressioni
Developmental stagei
Detected at low levels in fertilized and unfertilized eggs. Levels increased in two-cell embryos, decreased up to morula stage and were highest in blastocysts. Highly expressed in the inner cell mass of 3.5 day old blastocysts. Highly expressed in ectoderm and visceral endoderm at day 5.5. Detected throughout the brain and spinal cord at day 10 to 15. Detected in the basal layer of the epidermis after day 12.5, in particular on snout and distal on fore- and hindlimbs.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029920, Expressed in undifferentiated genital tubercle and 318 other tissues |
ExpressionAtlasi | Q04692, baseline and differential |
Genevisiblei | Q04692, MM |
Interactioni
Subunit structurei
Binds to DNA preferentially in the vicinity of transcriptional start sites.
Interacts with MSH2 and TRIM28.
Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2.
Interacts with PCNA (By similarity).
By similarityGO - Molecular functioni
- ubiquitin binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 199524, 8 interactors |
STRINGi | 10090.ENSMUSP00000031984 |
Miscellaneous databases
RNActi | Q04692, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 156 – 198 | CUE 1PROSITE-ProRule annotationAdd BLAST | 43 | |
Domaini | 247 – 290 | CUE 2PROSITE-ProRule annotationAdd BLAST | 44 | |
Domaini | 504 – 672 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 169 | |
Domaini | 853 – 1005 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 153 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 623 – 626 | DEGH box | 4 | |
Motifi | 716 – 733 | Nuclear localization signalSequence analysisAdd BLAST | 18 | |
Motifi | 1000 – 1003 | DEAD box | 4 |
Sequence similaritiesi
Belongs to the SNF2/RAD54 helicase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0389, Eukaryota |
GeneTreei | ENSGT00910000144252 |
HOGENOMi | CLU_000315_16_3_1 |
InParanoidi | Q04692 |
OMAi | HAKLIMK |
OrthoDBi | 61251at2759 |
PhylomeDBi | Q04692 |
TreeFami | TF105768 |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR003892, CUE IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51140, CUE, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q04692-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNLFNLDRFR FEKRSKIEEA PEAAPQPSQA RPSSPISLSA EEENAEGEGS
60 70 80 90 100
RANTPDSDVT EKTEDSSVPE PPDNERKASL SCFQNQRAIQ EYIDLSSDTE
110 120 130 140 150
DVSPNCSSTV QEKKFSKDTV IIVSEPSEDE ESHDLPSVTR RNDSSELEDL
160 170 180 190 200
SELEDLKDAK LQTLKELFPQ RSDSDLLKLI ESTSTMDGAI AAALLMFGDA
210 220 230 240 250
GGGPRKRKLS SSSEEDDVND DQSVKQPRGD RGEESNESAE ASSNWEKQES
260 270 280 290 300
IVLKLQKEFP NFDKQELREV LKEHEWMYTE ALESLKVFAE DQDVQCASQS
310 320 330 340 350
EVTNGKEVAR NQNYSKNATK IKMKQKISVK PQNGFNKKRK KNVFNPKKAV
360 370 380 390 400
EDSEYDSGSD AGSSLDEDYS SCEEVMEDGY KGKILHFLQV SSIAELTLIP
410 420 430 440 450
KCSQKKAQKI TELRPFNNWE ALFTKMSKIN GLSEDLIWNC KTVIQERDVV
460 470 480 490 500
IRLMNKCEDI SNKLTKQVTM LTGNGGGWNR EQPSLLNQSL SLKPYQKVGL
510 520 530 540 550
NWLALVHKHG LNGILADEMG LGKTIQAIAF LAYLFQEGNK GPHLIVVPAS
560 570 580 590 600
TIDNWLREVN LWCPSLNVLC YYGSQEERKQ IRFNIHNKYE DYNVIVTTYN
610 620 630 640 650
CAISSSDDRS LFRRLKLNYA IFDEGHMLKN MGSIRYQHLM TINARNRLLL
660 670 680 690 700
TGTPVQNNLL ELMSLLNFVM PHMFSSSTSE IRRMFSSKTK PADEQSIYEK
710 720 730 740 750
ERIAHAKQII KPFILRRVKE EVLKLLPPKK DRIELCAMSE KQEQLYSGLF
760 770 780 790 800
NRLKKSINNL EKNTEMCNVM MQLRKMANHP LLHRQYYTPE KLKEMSQLML
810 820 830 840 850
KEPTHCEANP DLIFEDMEVM TDFELHVLCK QYQHINSYQL DMDLILDSGK
860 870 880 890 900
FRALGCILSE LKQKGDRVVL FSQFTMMLDI LEVLLKHHQH RYLRLDGKTQ
910 920 930 940 950
ISERIHLIDE FNTDMDIFVF LLSTKAGGLG INLTSANVVI LHDIDCNPYN
960 970 980 990 1000
DKQAEDRCHR VGQTKEVLVI KLISQGTIEE SMLKINQQKL KLEQDMTTVD
1010 1020
EADEGSMPAD IATLLKTSMG L
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SUP7 | A0A0N4SUP7_MOUSE | SWI/SNF-related matrix-associated a... | Smarcad1 | 123 | Annotation score: | ||
A0A0N4SVP9 | A0A0N4SVP9_MOUSE | SWI/SNF-related matrix-associated a... | Smarcad1 | 134 | Annotation score: | ||
A0A0N4SUQ5 | A0A0N4SUQ5_MOUSE | SWI/SNF-related matrix-associated a... | Smarcad1 | 73 | Annotation score: | ||
A0A0N4SVS7 | A0A0N4SVS7_MOUSE | SWI/SNF-related matrix-associated a... | Smarcad1 | 68 | Annotation score: | ||
A0A0N4SVV9 | A0A0N4SVV9_MOUSE | SWI/SNF-related matrix-associated a... | Smarcad1 | 46 | Annotation score: |
Sequence cautioni
The sequence BAC65736 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA49560 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 857 | I → S in CAA49560 (PubMed:1489724).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007080 | 1 – 185 | Missing in isoform 2. 1 PublicationAdd BLAST | 185 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69942 mRNA Translation: CAA49560.1 Different initiation. AK122454 mRNA Translation: BAC65736.1 Different initiation. BC042442 mRNA Translation: AAH42442.1 AK134442 mRNA Translation: BAE22146.1 AK147884 mRNA Translation: BAE28202.1 |
CCDSi | CCDS20204.1 [Q04692-1] |
PIRi | A56559 |
RefSeqi | NP_001240321.1, NM_001253392.1 [Q04692-2] NP_031984.1, NM_007958.1 [Q04692-1] XP_006505572.1, XM_006505509.3 XP_006505573.1, XM_006505510.2 [Q04692-1] XP_006505575.1, XM_006505512.3 [Q04692-2] XP_011239507.1, XM_011241205.2 [Q04692-2] |
Genome annotation databases
Ensembli | ENSMUST00000031984; ENSMUSP00000031984; ENSMUSG00000029920 [Q04692-1] |
GeneIDi | 13990 |
KEGGi | mmu:13990 |
UCSCi | uc009ced.1, mouse [Q04692-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69942 mRNA Translation: CAA49560.1 Different initiation. AK122454 mRNA Translation: BAC65736.1 Different initiation. BC042442 mRNA Translation: AAH42442.1 AK134442 mRNA Translation: BAE22146.1 AK147884 mRNA Translation: BAE28202.1 |
CCDSi | CCDS20204.1 [Q04692-1] |
PIRi | A56559 |
RefSeqi | NP_001240321.1, NM_001253392.1 [Q04692-2] NP_031984.1, NM_007958.1 [Q04692-1] XP_006505572.1, XM_006505509.3 XP_006505573.1, XM_006505510.2 [Q04692-1] XP_006505575.1, XM_006505512.3 [Q04692-2] XP_011239507.1, XM_011241205.2 [Q04692-2] |
3D structure databases
SMRi | Q04692 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199524, 8 interactors |
STRINGi | 10090.ENSMUSP00000031984 |
PTM databases
iPTMneti | Q04692 |
PhosphoSitePlusi | Q04692 |
Proteomic databases
EPDi | Q04692 |
jPOSTi | Q04692 |
MaxQBi | Q04692 |
PaxDbi | Q04692 |
PeptideAtlasi | Q04692 |
PRIDEi | Q04692 |
Protocols and materials databases
Antibodypediai | 14709, 131 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031984; ENSMUSP00000031984; ENSMUSG00000029920 [Q04692-1] |
GeneIDi | 13990 |
KEGGi | mmu:13990 |
UCSCi | uc009ced.1, mouse [Q04692-1] |
Organism-specific databases
CTDi | 56916 |
MGIi | MGI:95453, Smarcad1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0389, Eukaryota |
GeneTreei | ENSGT00910000144252 |
HOGENOMi | CLU_000315_16_3_1 |
InParanoidi | Q04692 |
OMAi | HAKLIMK |
OrthoDBi | 61251at2759 |
PhylomeDBi | Q04692 |
TreeFami | TF105768 |
Miscellaneous databases
BioGRID-ORCSi | 13990, 2 hits in 23 CRISPR screens |
ChiTaRSi | Smarcad1, mouse |
PROi | PR:Q04692 |
RNActi | Q04692, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029920, Expressed in undifferentiated genital tubercle and 318 other tissues |
ExpressionAtlasi | Q04692, baseline and differential |
Genevisiblei | Q04692, MM |
Family and domain databases
Gene3Di | 3.40.50.10810, 1 hit |
InterProi | View protein in InterPro IPR003892, CUE IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF00176, SNF2_N, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51140, CUE, 2 hits PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SMRCD_MOUSE | |
Accessioni | Q04692Primary (citable) accession number: Q04692 Secondary accession number(s): Q3UGK6, Q3UYR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 28, 2003 |
Last sequence update: | March 28, 2003 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families