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UniProtKB - Q04692 (SMRCD_MOUSE)

Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

Gene

Smarcad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi516 – 524ATPPROSITE-ProRule annotation9
Nucleotide bindingi892 – 899ATPPROSITE-ProRule annotation8

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase SMARCAD1
Enhancer trap locus homolog 1
Short name:
Etl-1
Gene namesi
Name:Smarcad1
Synonyms:Etl1, Kiaa1122
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95453 Smarcad1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Deficient mice have reduced viability and show growth retardation, skeletal dysplasia and impaired fertility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743571 – 1021SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1Add BLAST1021

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei54PhosphothreonineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei79PhosphoserineBy similarity1
Modified residuei124PhosphoserineCombined sources1
Modified residuei127PhosphoserineCombined sources1
Modified residuei132PhosphoserineBy similarity1
Modified residuei144PhosphoserineCombined sources1
Modified residuei145PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei210PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei238PhosphoserineBy similarity1
Modified residuei298PhosphoserineBy similarity1
Cross-linki330Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki466Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki719Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki991Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ04692
MaxQBiQ04692
PaxDbiQ04692
PeptideAtlasiQ04692
PRIDEiQ04692

PTM databases

iPTMnetiQ04692
PhosphoSitePlusiQ04692

Expressioni

Developmental stagei

Detected at low levels in fertilized and unfertilized eggs. Levels increased in two-cell embryos, decreased up to morula stage and were highest in blastocysts. Highly expressed in the inner cell mass of 3.5 day old blastocysts. Highly expressed in ectoderm and visceral endoderm at day 5.5. Detected throughout the brain and spinal cord at day 10 to 15. Detected in the basal layer of the epidermis after day 12.5, in particular on snout and distal on fore- and hindlimbs.1 Publication

Gene expression databases

BgeeiENSMUSG00000029920 Expressed in 302 organ(s), highest expression level in embryonic stem cell
ExpressionAtlasiQ04692 baseline and differential
GenevisibleiQ04692 MM

Interactioni

Subunit structurei

Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA (By similarity).By similarity

Protein-protein interaction databases

BioGridi199524, 3 interactors
STRINGi10090.ENSMUSP00000031984

Structurei

3D structure databases

ProteinModelPortaliQ04692
SMRiQ04692
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 198CUE 1PROSITE-ProRule annotationAdd BLAST43
Domaini247 – 290CUE 2PROSITE-ProRule annotationAdd BLAST44
Domaini504 – 672Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini853 – 1005Helicase C-terminalPROSITE-ProRule annotationAdd BLAST153

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi623 – 626DEGH box4
Motifi716 – 733Nuclear localization signalSequence analysisAdd BLAST18
Motifi1000 – 1003DEAD box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0389 Eukaryota
ENOG410XNUT LUCA
GeneTreeiENSGT00910000144252
HOGENOMiHOG000172362
HOVERGENiHBG085229
InParanoidiQ04692
KOiK14439
OMAiSEFPNGK
OrthoDBiEOG091G0307
PhylomeDBiQ04692
TreeFamiTF105768

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR003892 CUE
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51140 CUE, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q04692-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNLFNLDRFR FEKRSKIEEA PEAAPQPSQA RPSSPISLSA EEENAEGEGS 
        60         70         80         90        100
RANTPDSDVT EKTEDSSVPE PPDNERKASL SCFQNQRAIQ EYIDLSSDTE 
       110        120        130        140        150
DVSPNCSSTV QEKKFSKDTV IIVSEPSEDE ESHDLPSVTR RNDSSELEDL 
       160        170        180        190        200
SELEDLKDAK LQTLKELFPQ RSDSDLLKLI ESTSTMDGAI AAALLMFGDA 
       210        220        230        240        250
GGGPRKRKLS SSSEEDDVND DQSVKQPRGD RGEESNESAE ASSNWEKQES 
       260        270        280        290        300
IVLKLQKEFP NFDKQELREV LKEHEWMYTE ALESLKVFAE DQDVQCASQS 
       310        320        330        340        350
EVTNGKEVAR NQNYSKNATK IKMKQKISVK PQNGFNKKRK KNVFNPKKAV 
       360        370        380        390        400
EDSEYDSGSD AGSSLDEDYS SCEEVMEDGY KGKILHFLQV SSIAELTLIP 
       410        420        430        440        450
KCSQKKAQKI TELRPFNNWE ALFTKMSKIN GLSEDLIWNC KTVIQERDVV 
       460        470        480        490        500
IRLMNKCEDI SNKLTKQVTM LTGNGGGWNR EQPSLLNQSL SLKPYQKVGL 
       510        520        530        540        550
NWLALVHKHG LNGILADEMG LGKTIQAIAF LAYLFQEGNK GPHLIVVPAS 
       560        570        580        590        600
TIDNWLREVN LWCPSLNVLC YYGSQEERKQ IRFNIHNKYE DYNVIVTTYN 
       610        620        630        640        650
CAISSSDDRS LFRRLKLNYA IFDEGHMLKN MGSIRYQHLM TINARNRLLL 
       660        670        680        690        700
TGTPVQNNLL ELMSLLNFVM PHMFSSSTSE IRRMFSSKTK PADEQSIYEK 
       710        720        730        740        750
ERIAHAKQII KPFILRRVKE EVLKLLPPKK DRIELCAMSE KQEQLYSGLF 
       760        770        780        790        800
NRLKKSINNL EKNTEMCNVM MQLRKMANHP LLHRQYYTPE KLKEMSQLML 
       810        820        830        840        850
KEPTHCEANP DLIFEDMEVM TDFELHVLCK QYQHINSYQL DMDLILDSGK 
       860        870        880        890        900
FRALGCILSE LKQKGDRVVL FSQFTMMLDI LEVLLKHHQH RYLRLDGKTQ 
       910        920        930        940        950
ISERIHLIDE FNTDMDIFVF LLSTKAGGLG INLTSANVVI LHDIDCNPYN 
       960        970        980        990       1000
DKQAEDRCHR VGQTKEVLVI KLISQGTIEE SMLKINQQKL KLEQDMTTVD 
      1010       1020 
EADEGSMPAD IATLLKTSMG L
Length:1,021
Mass (Da):116,451
Last modified:March 28, 2003 - v2
Checksum:iE3237AA2B135538A
GO
Isoform 2 (identifier: Q04692-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-185: Missing.

Note: No experimental confirmation available.
Show »
Length:836
Mass (Da):95,787
Checksum:iDD7B56518C73242B
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0N4SUP7A0A0N4SUP7_MOUSE
SWI/SNF-related matrix-associated a...
Smarcad1
123Annotation score:
A0A0N4SVP9A0A0N4SVP9_MOUSE
SWI/SNF-related matrix-associated a...
Smarcad1
134Annotation score:
A0A0N4SVV9A0A0N4SVV9_MOUSE
SWI/SNF-related matrix-associated a...
Smarcad1
46Annotation score:
A0A0N4SVS7A0A0N4SVS7_MOUSE
SWI/SNF-related matrix-associated a...
Smarcad1
68Annotation score:
A0A0N4SUQ5A0A0N4SUQ5_MOUSE
SWI/SNF-related matrix-associated a...
Smarcad1
73Annotation score:

Sequence cautioni

The sequence BAC65736 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA49560 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti857I → S in CAA49560 (PubMed:1489724).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0070801 – 185Missing in isoform 2. 1 PublicationAdd BLAST185

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69942 mRNA Translation: CAA49560.1 Different initiation.
AK122454 mRNA Translation: BAC65736.1 Different initiation.
BC042442 mRNA Translation: AAH42442.1
AK134442 mRNA Translation: BAE22146.1
AK147884 mRNA Translation: BAE28202.1
CCDSiCCDS20204.1 [Q04692-1]
PIRiA56559
RefSeqiNP_001240321.1, NM_001253392.1 [Q04692-2]
NP_031984.1, NM_007958.1 [Q04692-1]
XP_006505572.1, XM_006505509.3
XP_006505573.1, XM_006505510.2 [Q04692-1]
XP_006505575.1, XM_006505512.3 [Q04692-2]
XP_011239507.1, XM_011241205.2 [Q04692-2]
UniGeneiMm.99113

Genome annotation databases

EnsembliENSMUST00000031984; ENSMUSP00000031984; ENSMUSG00000029920 [Q04692-1]
GeneIDi13990
KEGGimmu:13990
UCSCiuc009ced.1 mouse [Q04692-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69942 mRNA Translation: CAA49560.1 Different initiation.
AK122454 mRNA Translation: BAC65736.1 Different initiation.
BC042442 mRNA Translation: AAH42442.1
AK134442 mRNA Translation: BAE22146.1
AK147884 mRNA Translation: BAE28202.1
CCDSiCCDS20204.1 [Q04692-1]
PIRiA56559
RefSeqiNP_001240321.1, NM_001253392.1 [Q04692-2]
NP_031984.1, NM_007958.1 [Q04692-1]
XP_006505572.1, XM_006505509.3
XP_006505573.1, XM_006505510.2 [Q04692-1]
XP_006505575.1, XM_006505512.3 [Q04692-2]
XP_011239507.1, XM_011241205.2 [Q04692-2]
UniGeneiMm.99113

3D structure databases

ProteinModelPortaliQ04692
SMRiQ04692
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199524, 3 interactors
STRINGi10090.ENSMUSP00000031984

PTM databases

iPTMnetiQ04692
PhosphoSitePlusiQ04692

Proteomic databases

EPDiQ04692
MaxQBiQ04692
PaxDbiQ04692
PeptideAtlasiQ04692
PRIDEiQ04692

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031984; ENSMUSP00000031984; ENSMUSG00000029920 [Q04692-1]
GeneIDi13990
KEGGimmu:13990
UCSCiuc009ced.1 mouse [Q04692-1]

Organism-specific databases

CTDi56916
MGIiMGI:95453 Smarcad1
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0389 Eukaryota
ENOG410XNUT LUCA
GeneTreeiENSGT00910000144252
HOGENOMiHOG000172362
HOVERGENiHBG085229
InParanoidiQ04692
KOiK14439
OMAiSEFPNGK
OrthoDBiEOG091G0307
PhylomeDBiQ04692
TreeFamiTF105768

Miscellaneous databases

ChiTaRSiSmarcad1 mouse
PROiPR:Q04692
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029920 Expressed in 302 organ(s), highest expression level in embryonic stem cell
ExpressionAtlasiQ04692 baseline and differential
GenevisibleiQ04692 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR003892 CUE
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51140 CUE, 2 hits
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSMRCD_MOUSE
AccessioniPrimary (citable) accession number: Q04692
Secondary accession number(s): Q3UGK6, Q3UYR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 28, 2003
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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