UniProtKB - Q04666 (HES1_RAT)
Protein
Transcription factor HES-1
Gene
Hes1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).By similarity1 Publication
GO - Molecular functioni
- chaperone binding Source: RGD
- chromatin binding Source: RGD
- DNA binding Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription repressor activity Source: RGD
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: UniProtKB
- E-box binding Source: RGD
- histone deacetylase binding Source: RGD
- HLH domain binding Source: RGD
- identical protein binding Source: RGD
- JUN kinase binding Source: RGD
- N-box binding Source: RGD
- protein-containing complex binding Source: RGD
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription factor binding Source: RGD
- sequence-specific DNA binding Source: BHF-UCL
- sequence-specific double-stranded DNA binding Source: RGD
- transcription factor binding Source: RGD
GO - Biological processi
- adenohypophysis development Source: RGD
- anterior/posterior pattern specification Source: GO_Central
- aorta morphogenesis Source: RGD
- artery morphogenesis Source: RGD
- ascending aorta morphogenesis Source: RGD
- cardiac neural crest cell development involved in outflow tract morphogenesis Source: RGD
- cell adhesion Source: RGD
- cell fate commitment Source: RGD
- cell maturation Source: RGD
- cell migration Source: RGD
- cell morphogenesis involved in neuron differentiation Source: RGD
- cellular response to fatty acid Source: RGD
- cellular response to interleukin-1 Source: RGD
- cellular response to nerve growth factor stimulus Source: RGD
- cellular response to tumor necrosis factor Source: RGD
- cochlea development Source: RGD
- comma-shaped body morphogenesis Source: RGD
- common bile duct development Source: RGD
- embryonic heart tube morphogenesis Source: RGD
- establishment of epithelial cell polarity Source: RGD
- forebrain radial glial cell differentiation Source: UniProtKB
- glomerulus vasculature development Source: RGD
- hair cell differentiation Source: RGD
- hindbrain morphogenesis Source: RGD
- inner ear receptor cell stereocilium organization Source: RGD
- in utero embryonic development Source: RGD
- labyrinthine layer blood vessel development Source: RGD
- lateral inhibition Source: RGD
- liver development Source: RGD
- lung development Source: RGD
- metanephric nephron tubule morphogenesis Source: RGD
- midbrain development Source: RGD
- midbrain-hindbrain boundary morphogenesis Source: RGD
- negative regulation of calcium ion import Source: RGD
- negative regulation of cell differentiation Source: RGD
- negative regulation of cell fate determination Source: RGD
- negative regulation of DNA-binding transcription factor activity Source: RGD
- negative regulation of forebrain neuron differentiation Source: UniProtKB
- negative regulation of gene expression Source: RGD
- negative regulation of glial cell proliferation Source: UniProtKB
- negative regulation of inner ear auditory receptor cell differentiation Source: RGD
- negative regulation of inner ear receptor cell differentiation Source: RGD
- negative regulation of neurogenesis Source: RGD
- negative regulation of neuron differentiation Source: RGD
- negative regulation of neuron projection development Source: RGD
- negative regulation of oligodendrocyte differentiation Source: RGD
- negative regulation of pancreatic A cell differentiation Source: RGD
- negative regulation of pro-B cell differentiation Source: UniProtKB
- negative regulation of stem cell differentiation Source: RGD
- negative regulation of stomach neuroendocrine cell differentiation Source: RGD
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- neural tube development Source: RGD
- neuronal stem cell population maintenance Source: UniProtKB
- neuron differentiation Source: RGD
- Notch signaling pathway Source: RGD
- oculomotor nerve development Source: RGD
- outflow tract morphogenesis Source: RGD
- pancreas development Source: RGD
- pattern specification process Source: RGD
- pharyngeal arch artery morphogenesis Source: RGD
- pituitary gland development Source: RGD
- positive regulation of astrocyte differentiation Source: UniProtKB
- positive regulation of BMP signaling pathway Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of DNA binding Source: UniProtKB
- positive regulation of gene expression Source: RGD
- positive regulation of glial cell differentiation Source: RGD
- positive regulation of mitotic cell cycle, embryonic Source: RGD
- positive regulation of Notch signaling pathway Source: RGD
- positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
- positive regulation of T cell proliferation Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
- protein-containing complex assembly Source: UniProtKB
- regulation of epithelial cell proliferation Source: RGD
- regulation of fat cell differentiation Source: RGD
- regulation of neurogenesis Source: RGD
- regulation of protein-containing complex assembly Source: RGD
- regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
- regulation of secondary heart field cardioblast proliferation Source: RGD
- regulation of timing of cell differentiation Source: RGD
- regulation of timing of neuron differentiation Source: RGD
- regulation of transcription by RNA polymerase II Source: RGD
- renal interstitial fibroblast development Source: RGD
- response to alkaloid Source: RGD
- response to organic cyclic compound Source: RGD
- response to thyroid hormone Source: RGD
- smoothened signaling pathway Source: RGD
- somatic stem cell population maintenance Source: RGD
- S-shaped body morphogenesis Source: RGD
- telencephalon development Source: RGD
- thymus development Source: RGD
- trochlear nerve development Source: RGD
- ureteric bud morphogenesis Source: RGD
- vascular associated smooth muscle cell development Source: RGD
- ventricular septum development Source: RGD
- ventricular septum morphogenesis Source: RGD
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor HES-1Alternative name(s): Hairy and enhancer of split 1 Hairy-like protein RHL |
Gene namesi | Name:Hes1 Synonyms:Hes-1, Hl |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 62081, Hes1 |
Subcellular locationi
Nucleus
Nucleus
- nuclear matrix Source: RGD
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- chromatin Source: RGD
- cytoplasm Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127204 | 1 – 281 | Transcription factor HES-1Add BLAST | 281 |
Proteomic databases
PaxDbi | Q04666 |
PRIDEi | Q04666 |
PTM databases
iPTMneti | Q04666 |
PhosphoSitePlusi | Q04666 |
Expressioni
Tissue specificityi
Present in all tissues examined but highest in epithelial cells and in mesoderm-derived tissues such as embryonal muscle cells.
Interactioni
Subunit structurei
Interacts with SIRT1.
Interacts weakly with TLE2.
Interacts with HES6 (By similarity). Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.
Interacts (via WPRW motif) with TLE1.
Interacts with an FA complex, composed of FANCA, FANCF, FANCG and FANCL, but not of FANCC, nor FANCE (By similarity).
By similarityGO - Molecular functioni
- chaperone binding Source: RGD
- histone deacetylase binding Source: RGD
- HLH domain binding Source: RGD
- identical protein binding Source: RGD
- JUN kinase binding Source: RGD
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II transcription factor binding Source: RGD
- transcription factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248210, 8 interactors |
CORUMi | Q04666 |
ELMi | Q04666 |
IntActi | Q04666, 3 interactors |
MINTi | Q04666 |
STRINGi | 10116.ENSRNOP00000002346 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 91 | bHLHPROSITE-ProRule annotationAdd BLAST | 58 | |
Domaini | 110 – 143 | OrangePROSITE-ProRule annotationAdd BLAST | 34 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 276 – 279 | WRPW motif | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 156 – 247 | Pro-richAdd BLAST | 92 | |
Compositional biasi | 250 – 274 | Ser/Thr-richAdd BLAST | 25 |
Domaini
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).1 Publication
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.1 Publication
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.By similarity
Phylogenomic databases
eggNOGi | KOG4304, Eukaryota |
InParanoidi | Q04666 |
OrthoDBi | 1427802at2759 |
PhylomeDBi | Q04666 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR003650, Orange_dom |
Pfami | View protein in Pfam PF07527, Hairy_orange, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00511, ORANGE, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS51054, ORANGE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q04666-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI
60 70 80 90 100
NESLSQLKTL ILDALKKDSS RHSKLEKADI LEMTVKHLRN LQRAQMTAAL
110 120 130 140 150
STDPSVLGKY RAGFSECMNE VTRFLSTCEG VNTEVRTRLL GHLANCMTQI
160 170 180 190 200
NAMTYPGQAH PALQAPPPPP PSGPGGPQHA PFAPPPPLVP IPGGAAPPPG
210 220 230 240 250
SAPCKLGSQA GEAAKVFGGF QVVPAPDGQF AFLIPNGAFA HSGPVIPVYT
260 270 280
SNSGTSVGPN AVSPSSGSSL TADSMWRPWR N
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD4ADT9 | D4ADT9_RAT | Transcription factor HES-1 | Hes1 | 281 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 86 | K → N in AAA41307 (PubMed:8417318).Curated | 1 | |
Sequence conflicti | 93 | R → W in AAA41307 (PubMed:8417318).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13417 mRNA Translation: BAA02682.1 L04527 mRNA Translation: AAA41307.1 BC061730 mRNA Translation: AAH61730.1 |
PIRi | S36748 |
RefSeqi | NP_077336.3, NM_024360.3 |
Genome annotation databases
GeneIDi | 29577 |
KEGGi | rno:29577 |
UCSCi | RGD:62081, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13417 mRNA Translation: BAA02682.1 L04527 mRNA Translation: AAA41307.1 BC061730 mRNA Translation: AAH61730.1 |
PIRi | S36748 |
RefSeqi | NP_077336.3, NM_024360.3 |
3D structure databases
BMRBi | Q04666 |
SMRi | Q04666 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248210, 8 interactors |
CORUMi | Q04666 |
ELMi | Q04666 |
IntActi | Q04666, 3 interactors |
MINTi | Q04666 |
STRINGi | 10116.ENSRNOP00000002346 |
Chemistry databases
ChEMBLi | CHEMBL4105998 |
PTM databases
iPTMneti | Q04666 |
PhosphoSitePlusi | Q04666 |
Proteomic databases
PaxDbi | Q04666 |
PRIDEi | Q04666 |
Genome annotation databases
GeneIDi | 29577 |
KEGGi | rno:29577 |
UCSCi | RGD:62081, rat |
Organism-specific databases
CTDi | 3280 |
RGDi | 62081, Hes1 |
Phylogenomic databases
eggNOGi | KOG4304, Eukaryota |
InParanoidi | Q04666 |
OrthoDBi | 1427802at2759 |
PhylomeDBi | Q04666 |
Miscellaneous databases
PROi | PR:Q04666 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR003650, Orange_dom |
Pfami | View protein in Pfam PF07527, Hairy_orange, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00511, ORANGE, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS51054, ORANGE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HES1_RAT | |
Accessioni | Q04666Primary (citable) accession number: Q04666 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |