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Protein

Transcription factor HES-1

Gene

Hes1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage (By similarity).By similarity1 Publication

GO - Molecular functioni

  • chromatin binding Source: RGD
  • DNA binding Source: RGD
  • E-box binding Source: RGD
  • JUN kinase binding Source: RGD
  • N-box binding Source: RGD
  • protein-containing complex binding Source: RGD
  • protein homodimerization activity Source: RGD
  • sequence-specific DNA binding Source: BHF-UCL
  • transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: UniProtKB
  • transcription factor binding Source: RGD

GO - Biological processi

  • cellular response to fatty acid Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cellular response to nerve growth factor stimulus Source: RGD
  • cellular response to tumor necrosis factor Source: RGD
  • forebrain radial glial cell differentiation Source: UniProtKB
  • hair cell differentiation Source: RGD
  • negative regulation of calcium ion import Source: RGD
  • negative regulation of forebrain neuron differentiation Source: UniProtKB
  • negative regulation of gene expression Source: RGD
  • negative regulation of glial cell proliferation Source: UniProtKB
  • negative regulation of neurogenesis Source: RGD
  • negative regulation of neuron projection development Source: RGD
  • negative regulation of oligodendrocyte differentiation Source: RGD
  • negative regulation of pro-B cell differentiation Source: UniProtKB
  • negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: RGD
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • neuronal stem cell population maintenance Source: UniProtKB
  • neuron differentiation Source: RGD
  • positive regulation of astrocyte differentiation Source: UniProtKB
  • positive regulation of DNA binding Source: UniProtKB
  • positive regulation of gene expression Source: RGD
  • positive regulation of glial cell differentiation Source: RGD
  • positive regulation of JAK-STAT cascade Source: UniProtKB
  • positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
  • protein-containing complex assembly Source: UniProtKB
  • regulation of JAK-STAT cascade Source: UniProtKB
  • regulation of protein heterodimerization activity Source: RGD
  • response to alkaloid Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to thyroid hormone Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HES-1
Alternative name(s):
Hairy and enhancer of split 1
Hairy-like protein
RHL
Gene namesi
Name:Hes1
Synonyms:Hes-1, Hl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62081 Hes1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272041 – 281Transcription factor HES-1Add BLAST281

Proteomic databases

PaxDbiQ04666

PTM databases

iPTMnetiQ04666
PhosphoSitePlusiQ04666

Expressioni

Tissue specificityi

Present in all tissues examined but highest in epithelial cells and in mesoderm-derived tissues such as embryonal muscle cells.

Interactioni

Subunit structurei

Interacts with SIRT1. Interacts weakly with TLE2. Interacts with HES6 (By similarity). Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts (via WPRW motif) with TLE1. Interacts with an FA complex, composed of FANCA, FANCF, FANCG and FANCL, but not of FANCC, nor FANCE (By similarity).By similarity

GO - Molecular functioni

  • JUN kinase binding Source: RGD
  • protein homodimerization activity Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi248210, 7 interactors
CORUMiQ04666
ELMiQ04666
IntActiQ04666, 3 interactors
MINTiQ04666
STRINGi10116.ENSRNOP00000002346

Structurei

3D structure databases

ProteinModelPortaliQ04666
SMRiQ04666
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 91bHLHPROSITE-ProRule annotationAdd BLAST58
Domaini110 – 143OrangePROSITE-ProRule annotationAdd BLAST34

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi276 – 279WRPW motif4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi156 – 247Pro-richAdd BLAST92
Compositional biasi250 – 274Ser/Thr-richAdd BLAST25

Domaini

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).1 Publication
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.1 Publication
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.By similarity

Phylogenomic databases

eggNOGiKOG4304 Eukaryota
ENOG4111F0X LUCA
HOVERGENiHBG005960
InParanoidiQ04666
KOiK06054
PhylomeDBiQ04666

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR003650 Orange_dom
PfamiView protein in Pfam
PF07527 Hairy_orange, 1 hit
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00511 ORANGE, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS51054 ORANGE, 1 hit

Sequencei

Sequence statusi: Complete.

Q04666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI
60 70 80 90 100
NESLSQLKTL ILDALKKDSS RHSKLEKADI LEMTVKHLRN LQRAQMTAAL
110 120 130 140 150
STDPSVLGKY RAGFSECMNE VTRFLSTCEG VNTEVRTRLL GHLANCMTQI
160 170 180 190 200
NAMTYPGQAH PALQAPPPPP PSGPGGPQHA PFAPPPPLVP IPGGAAPPPG
210 220 230 240 250
SAPCKLGSQA GEAAKVFGGF QVVPAPDGQF AFLIPNGAFA HSGPVIPVYT
260 270 280
SNSGTSVGPN AVSPSSGSSL TADSMWRPWR N
Length:281
Mass (Da):29,622
Last modified:June 1, 1994 - v1
Checksum:i8A98C8964F075B0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86K → N in AAA41307 (PubMed:8417318).Curated1
Sequence conflicti93R → W in AAA41307 (PubMed:8417318).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13417 mRNA Translation: BAA02682.1
L04527 mRNA Translation: AAA41307.1
BC061730 mRNA Translation: AAH61730.1
PIRiS36748
RefSeqiNP_077336.3, NM_024360.3
UniGeneiRn.19727

Genome annotation databases

GeneIDi29577
KEGGirno:29577
UCSCiRGD:62081 rat

Similar proteinsi

Entry informationi

Entry nameiHES1_RAT
AccessioniPrimary (citable) accession number: Q04666
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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