Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein alpha-2 subunit

Gene

gpa2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. This protein may be involved in the determination of the cAMP level according to nutritional conditions, most probably as a regulator of adenylyl cyclase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48MagnesiumBy similarity1
Metal bindingi179MagnesiumBy similarity1
Binding sitei326GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 48GTPBy similarity8
Nucleotide bindingi173 – 179GTPBy similarity7
Nucleotide bindingi198 – 202GTPBy similarity5
Nucleotide bindingi269 – 272GTPBy similarity4

GO - Molecular functioni

  • adenylate cyclase activator activity Source: PomBase
  • G-protein beta/gamma-subunit complex binding Source: GO_Central
  • G-protein coupled glucose receptor activity Source: PomBase
  • G-protein coupled receptor binding Source: GO_Central
  • GTPase activity Source: GO_Central
  • GTP binding Source: PomBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adenylate cyclase-activating glucose-activated G-protein coupled receptor signaling pathway Source: PomBase
  • carbon catabolite repression of transcription from RNA polymerase II promoter by glucose Source: PomBase
  • negative regulation of induction of conjugation with cellular fusion Source: PomBase
  • negative regulation of transcription by RNA polymerase II Source: PomBase
  • signal transduction involved in regulation of aerobic respiration Source: PomBase

Keywordsi

Molecular functionTransducer
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein alpha-2 subunit
Alternative name(s):
GP2-alpha
Gene namesi
Name:gpa2
ORF Names:SPAC23H3.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23H3.13c
PomBaseiSPAC23H3.13c gpa2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176R → H: Decrease in mating and sporulation efficiency. 1 Publication1
Mutagenesisi202Q → L: Decrease in mating and sporulation efficiency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002036091 – 354Guanine nucleotide-binding protein alpha-2 subunitAdd BLAST354

Proteomic databases

MaxQBiQ04665
PaxDbiQ04665
PRIDEiQ04665

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi278214, 81 interactors
STRINGi4896.SPAC23H3.13c.1

Structurei

3D structure databases

ProteinModelPortaliQ04665
SMRiQ04665
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family.Curated

Phylogenomic databases

HOGENOMiHOG000038730
InParanoidiQ04665
KOiK04630
OMAiFEHQYVN
OrthoDBiEOG092C25Q3
PhylomeDBiQ04665

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

Q04665-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIFNGLSES GESKKLNSKI EKQIENASKK DKKIYKVLLL GASDSGKSTI
60 70 80 90 100
SKQIKILNKN GFSQEEIMTF IPVIRRNLLE SAKTLVKIIV QKGINLDPLG
110 120 130 140 150
THNCEIIEKF NPTPGELINA NIGQAITSLW SANSVRSCTY GNDSVLIDSA
160 170 180 190 200
PYFFSRADEI CSRHYVPTID DILRSRNSTL GISEISFTLD HLQIRMFDVG
210 220 230 240 250
GQRTERRKWI YCFENVNSII FCVSLNDYDK KLYERAAPER NRLVESISLF
260 270 280 290 300
DSIINSQWFM HSSIILFLNK FDLFRKKLEH VPFQDYFPQY EGKNSVKSIT
310 320 330 340 350
RYILWLFVNP SINRAKHNIY PHITTAVDTS NIKVVFSAVK ETILQHSLKE

AGMF
Length:354
Mass (Da):40,523
Last modified:June 1, 1994 - v1
Checksum:iA0042174C1389B8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13366 Genomic DNA Translation: BAA02630.1
CU329670 Genomic DNA Translation: CAB16244.1
PIRiA46393
T38306
RefSeqiNP_593803.1, NM_001019232.2

Genome annotation databases

EnsemblFungiiSPAC23H3.13c.1; SPAC23H3.13c.1:pep; SPAC23H3.13c
GeneIDi2541720
KEGGispo:SPAC23H3.13c

Similar proteinsi

Entry informationi

Entry nameiGPA2_SCHPO
AccessioniPrimary (citable) accession number: Q04665
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health