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Protein

DNA polymerase epsilon subunit D

Gene

DPB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. Also functions as component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. The ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex.6 Publications

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 2100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29721-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit D (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit D
Gene namesi
Name:DPB4
Ordered Locus Names:YDR121W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002528 DPB4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001917541 – 196DNA polymerase epsilon subunit DAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183Phosphoserine; by ATM or ATRCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04603
PaxDbiQ04603
PRIDEiQ04603

PTM databases

iPTMnetiQ04603

Interactioni

Subunit structurei

DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4. Component of the ISW2 complex, which at least consists of ISW2, ITC1, DLS1 and DPB4.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DLS1P403663EBI-29938,EBI-25910

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32177, 153 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
CPX-728 ISW2 chromatin remodeling complex
DIPiDIP-4621N
IntActiQ04603, 21 interactors
MINTiQ04603
STRINGi4932.YDR121W

Structurei

3D structure databases

ProteinModelPortaliQ04603
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000112253
InParanoidiQ04603
KOiK02326
OMAiGWRKDAQ
OrthoDBiEOG092C4T63

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
SUPFAMiSSF47113 SSF47113, 1 hit

Sequencei

Sequence statusi: Complete.

Q04603-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPPKGWRKDA QGNYPTTSYI KEQENITIQD LLFPKSTIVN LAREVPQQSG
60 70 80 90 100
KKLLINKDAS LALQRGATVF VNHLLLFARE IAKSQDKKSC SVDDVLSALD
110 120 130 140 150
HIGHSALKGP VRDKLDEYQA AVEQRKKEKL DSGEVDADGD IDMGEDKENV
160 170 180 190
PVEKVKEHDE IEEQGDALQD VEESSEKKQK TESQDVETRV QNLEQT
Length:196
Mass (Da):21,997
Last modified:November 1, 1996 - v1
Checksum:i5D061EEA836B8BD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA Translation: CAA88674.1
AY557673 Genomic DNA Translation: AAS55999.1
BK006938 Genomic DNA Translation: DAA11967.1
PIRiS52686
RefSeqiNP_010406.3, NM_001180429.3

Genome annotation databases

EnsemblFungiiYDR121W; YDR121W; YDR121W
GeneIDi851699
KEGGisce:YDR121W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48758 Genomic DNA Translation: CAA88674.1
AY557673 Genomic DNA Translation: AAS55999.1
BK006938 Genomic DNA Translation: DAA11967.1
PIRiS52686
RefSeqiNP_010406.3, NM_001180429.3

3D structure databases

ProteinModelPortaliQ04603
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32177, 153 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
CPX-728 ISW2 chromatin remodeling complex
DIPiDIP-4621N
IntActiQ04603, 21 interactors
MINTiQ04603
STRINGi4932.YDR121W

PTM databases

iPTMnetiQ04603

Proteomic databases

MaxQBiQ04603
PaxDbiQ04603
PRIDEiQ04603

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR121W; YDR121W; YDR121W
GeneIDi851699
KEGGisce:YDR121W

Organism-specific databases

SGDiS000002528 DPB4

Phylogenomic databases

HOGENOMiHOG000112253
InParanoidiQ04603
KOiK02326
OMAiGWRKDAQ
OrthoDBiEOG092C4T63

Enzyme and pathway databases

BioCyciYEAST:G3O-29721-MONOMER

Miscellaneous databases

PROiPR:Q04603

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
SUPFAMiSSF47113 SSF47113, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPB4_YEAST
AccessioniPrimary (citable) accession number: Q04603
Secondary accession number(s): D6VSA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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