apxIIIB

Functionⁱ

Involved in the transport of the toxin RTX-III.

Miscellaneous

The sequence shown is that of serotype 2.

Caution

Tyr-13 is present instead of the conserved Cys which is expected to be the active site residue of peptidase C39. Thus they are presumed to be without peptidase activity.Curated

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	87	PROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	506 – 513	ATPPROSITE-ProRule annotation		8

GO - Molecular functionⁱ

ATPase activity, coupled to transmembrane movement of substances Source: InterPro
ATP binding Source: UniProtKB-KW
peptidase activity Source: InterPro
protein transporter activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cytolysis Source: UniProtKB-KW
protein secretion by the type I secretion system Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cytolysis, Transport
Ligand	ATP-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Toxin RTX-III translocation ATP-binding protein Alternative name(s): APX-IIIB Cytolysin IIIB Short name: CLY-IIIB RTX-III toxin determinant B
Gene namesⁱ	Name:apxIIIB Synonyms:clyIIIB, rtxB
Organismⁱ	Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Taxonomic identifierⁱ	715 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Subcellular locationⁱ

Cell membrane ; Multi-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	162 – 182	HelicalPROSITE-ProRule annotationAdd BLAST	21
Transmembraneⁱ	195 – 215	HelicalPROSITE-ProRule annotationAdd BLAST	21
Transmembraneⁱ	273 – 293	HelicalPROSITE-ProRule annotationAdd BLAST	21
Transmembraneⁱ	299 – 319	HelicalPROSITE-ProRule annotationAdd BLAST	21
Transmembraneⁱ	392 – 412	HelicalPROSITE-ProRule annotationAdd BLAST	21

GO - Cellular componentⁱ

integral component of membrane Source: UniProtKB-KW
plasma membrane Source: UniProtKB-SubCell
type I protein secretion system complex Source: InterPro

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000092391	1 – 711	Toxin RTX-III translocation ATP-binding proteinAdd BLAST		711

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q04473
SMRⁱ	Q04473
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1 – 129	Peptidase C39PROSITE-ProRule annotationAdd BLAST	129
Domainⁱ	158 – 440	ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST	283
Domainⁱ	472 – 707	ABC transporterPROSITE-ProRule annotationAdd BLAST	236

Sequence similaritiesⁱ

Belongs to the ABC transporter superfamily. Protein-1 exporter (TC 3.A.1.109) family. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02417 Peptidase_C39_likeA, 1 hit
Gene3Dⁱ	1.20.1560.10, 1 hit
InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR011527 ABC1_TM_dom IPR036640 ABC1_TM_sf IPR003439 ABC_transporter-like IPR017871 ABC_transporter_CS IPR010132 ATPase_T1SS_HlyB IPR027417 P-loop_NTPase IPR005074 Peptidase_C39 IPR039395 Peptidase_C39_likeA
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00664 ABC_membrane, 1 hit PF00005 ABC_tran, 1 hit PF03412 Peptidase_C39, 1 hit
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF90123 SSF90123, 1 hit
TIGRFAMsⁱ	TIGR01846 type_I_sec_HlyB, 1 hit
PROSITEⁱ	View protein in PROSITE PS50929 ABC_TM1F, 1 hit PS00211 ABC_TRANSPORTER_1, 1 hit PS50893 ABC_TRANSPORTER_2, 1 hit PS50990 PEPTIDASE_C39, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q04473-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MESQMPFNEK IDYGLHALVI LAQYHNVAVN PEEVKHKFDL DGKGLDLVAW 
        60         70         80         90        100
LLAAKSLELK MKRVKKSIER LPFIHLPALI WRDDGQHVIL MKIDTQTNRY 
       110        120        130        140        150
LIFDLEERNP KVLSAAEFHE IFQGGMILIT SRASIMGQLA KFDFTWFIPA 
       160        170        180        190        200
VIKYRKIFVE TIIVSIFLQL FALITPLFFQ VVMDKVLVHR GFSTLNVITV 
       210        220        230        240        250
ALSVVVIFEI VLSGLRTYIF SHSTSRIDVE LGAKLFRHLL ALPISYFENR 
       260        270        280        290        300
RVGDTVARVR ELDQIRNFLT GQALTSVLDL LFSFIFFAVM WYYSPKLTIV 
       310        320        330        340        350
ILLSLPCYIA WSIFISPILR RRLDEKFARN ADNQSFLVES VSAIDTIKAL 
       360        370        380        390        400
AVTPQMTNIW DKQLASYVSA DFRVTVLATI GQQGVQLIQK TVMIINLWLG 
       410        420        430        440        450
AHLVISGDLS IGQLITFNML SGQVIAPVVR LAQLWQDFQQ VGISITRLGD 
       460        470        480        490        500
VLNSPTENYQ GKLSLPEIFG DIAFKHIRFR YKPDAPIILD DVNLSVKQGE 
       510        520        530        540        550
VIGIVGRSGS GKSTLTKLLQ RFYIPENGQV LIDGHDLALA DPNWLRRQIG 
       560        570        580        590        600
VVLQDNVLLN RSIRDNIALT DPSMSMERVI YAAKLAGAHD FISELREGYN 
       610        620        630        640        650
TIVGELGAGL SGGQRQRIAI ARALVNNPRI LIFDEATSAL DYESEHIIMQ 
       660        670        680        690        700
NMQKICHGRT VIIIAHRLST VKNADRIIVM EKGHIVEQGK HNQLLENENG 
       710 
LYYYLNQLQS N

Length:711

Mass (Da):80,406

Last modified:November 1, 1995 - v1

Checksum:ⁱ5B8E46B889D12D92

GO

Sequence cautionⁱ

The sequence CAA48712 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	61	M → A in serotype 8.	1
Natural variantⁱ	91	M → T in serotype 8.	1
Natural variantⁱ	125 – 126	GM → DV in serotype 8.	2
Natural variantⁱ	213	S → N in serotype 8.	1
Natural variantⁱ	416	T → A in serotype 8.	1
Natural variantⁱ	469	F → K in serotype 8.	1
Natural variantⁱ	606	L → Q in serotype 8.	1
Natural variantⁱ	638	S → R in serotype 8.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L12145 Genomic DNA Translation: AAA21925.1 X80055 Genomic DNA Translation: CAA56359.1 X68815 Genomic DNA Translation: CAA48712.1 Different initiation.
PIRⁱ	S51785 C49219

Protein	Similar proteins		Species	Score	Length	Source
Q04473	Toxin (Fragment)		ACTPL		68	UniRef100_Q04473

Protein	Similar proteins		Species	Score	Length	Source
Q04473	Exotoxin translocation ATP-binding protein PaxB		PASAE		711	UniRef90_Q04473
Toxin (Fragment)		ACTPL		68
Type I secretion system permease/ATPase		Actinobacillus seminis		711
Toxin RTX-III translocation ATP-binding protein (RTX-III toxin determinant B)		ACTPJ		707
Toxin RTX-III translocation ATP-binding protein (RTX-III toxin determinant B)		Actinobacillus pleuropneumoniae serovar 6 str. Femo		707
+9

Protein	Similar proteins		Species	Score	Length	Source
Q04473	Toxin RTX-I translocation ATP-binding protein		ACTPL		707	UniRef50_P26760
Leukotoxin export ATP-binding protein LtxB		AGGAC		707
Leukotoxin translocation ATP-binding protein LktB		MANHA		708
Leukotoxin translocation ATP-binding protein LktB		MANGL		708
Leukotoxin translocation ATP-binding protein LktB		BIBTR		708
+1939

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L12145 Genomic DNA Translation: AAA21925.1 X80055 Genomic DNA Translation: CAA56359.1 X68815 Genomic DNA Translation: CAA48712.1 Different initiation.
PIRⁱ	S51785 C49219

3D structure databases

ProteinModelPortalⁱ	Q04473
SMRⁱ	Q04473
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

CDDⁱ	cd02417 Peptidase_C39_likeA, 1 hit
Gene3Dⁱ	1.20.1560.10, 1 hit
InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR011527 ABC1_TM_dom IPR036640 ABC1_TM_sf IPR003439 ABC_transporter-like IPR017871 ABC_transporter_CS IPR010132 ATPase_T1SS_HlyB IPR027417 P-loop_NTPase IPR005074 Peptidase_C39 IPR039395 Peptidase_C39_likeA
Pfamⁱ	View protein in Pfam PF00664 ABC_membrane, 1 hit PF00005 ABC_tran, 1 hit PF03412 Peptidase_C39, 1 hit
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF90123 SSF90123, 1 hit
TIGRFAMsⁱ	TIGR01846 type_I_sec_HlyB, 1 hit
PROSITEⁱ	View protein in PROSITE PS50929 ABC_TM1F, 1 hit PS00211 ABC_TRANSPORTER_1, 1 hit PS50893 ABC_TRANSPORTER_2, 1 hit PS50990 PEPTIDASE_C39, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RTX3B_ACTPL
Accessionⁱ	Q04473Primary (citable) accession number: Q04473
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
	Last sequence update:	November 1, 1995
	Last modified:	September 12, 2018
	This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Documents

SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q04473 (RTX3B_ACTPL)

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Toxin RTX-III translocation ATP-binding protein

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Caution

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Natural variant

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ