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Protein

ATP-dependent DNA helicase II subunit 2

Gene

YKU80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.14 Publications

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32803-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase II subunit 2 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent DNA helicase II subunit Ku80
High affinity DNA-binding factor subunit 2
Yeast Ku80
Gene namesi
Name:YKU80
Synonyms:HDF2
Ordered Locus Names:YMR106C
ORF Names:YM9718.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

SGDiS000004712 YKU80

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000843391 – 629ATP-dependent DNA helicase II subunit 2Add BLAST629

Proteomic databases

MaxQBiQ04437
PaxDbiQ04437
PRIDEiQ04437

Interactioni

Subunit structurei

Heterodimer of YKU70/HDF1 and YKU80/HDF2. Interacts with SIR4.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi35281, 289 interactors
ComplexPortaliCPX-1732 Ku70:Ku80 complex
DIPiDIP-2757N
IntActiQ04437, 115 interactors
MINTiQ04437
STRINGi4932.YMR106C

Structurei

Secondary structure

1629
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ04437
SMRiQ04437
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini254 – 476KuAdd BLAST223

Sequence similaritiesi

Belongs to the ku80 family.Curated

Phylogenomic databases

HOGENOMiHOG000066046
InParanoidiQ04437
KOiK10885
OMAiINVEGYP
OrthoDBiEOG092C1B2W

Family and domain databases

Gene3Di2.40.290.10, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR006164 Ku70/Ku80_beta-barrel_dom
IPR024193 Ku80
IPR005161 Ku_N
IPR016194 SPOC-like_C_dom_sf
IPR036465 vWFA_dom_sf
PANTHERiPTHR12604:SF4 PTHR12604:SF4, 1 hit
PfamiView protein in Pfam
PF02735 Ku, 1 hit
PF03731 Ku_N, 1 hit
PIRSFiPIRSF016570 Ku80, 1 hit
SMARTiView protein in SMART
SM00559 Ku78, 1 hit
SUPFAMiSSF100939 SSF100939, 1 hit
SSF53300 SSF53300, 1 hit

Sequencei

Sequence statusi: Complete.

Q04437-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSESTTFIV DVSPSMMKNN NVSKSMAYLE YTLLNKSKKS RKTDWISCYL
60 70 80 90 100
ANCPVSENSQ EIPNVFQIQS FLAPVTTTAT IGFIKRLKQY CDQHSHDSSN
110 120 130 140 150
EGLQSMIQCL LVVSLDIKQQ FQARKILKQI VVFTDNLDDL DITDEEIDLL
160 170 180 190 200
TEELSTRIIL IDCGKDTQEE RKKSNWLKLV EAIPNSRIYN MNELLVEITS
210 220 230 240 250
PATSVVKPVR VFSGELRLGA DILSTQTSNP SGSMQDENCL CIKVEAFPAT
260 270 280 290 300
KAVSGLNRKT AVEVEDSQKK ERYVGVKSII EYEIHNEGNK KNVSEDDQSG
310 320 330 340 350
SSYIPVTISK DSVTKAYRYG ADYVVLPSVL VDQTVYESFP GLDLRGFLNR
360 370 380 390 400
EALPRYFLTS ESSFITADTR LGCQSDLMAF SALVDVMLEN RKIAVARYVS
410 420 430 440 450
KKDSEVNMCA LCPVLIEHSN INSEKKFVKS LTLCRLPFAE DERVTDFPKL
460 470 480 490 500
LDRTTTSGVP LKKETDGHQI DELMEQFVDS MDTDELPEIP LGNYYQPIGE
510 520 530 540 550
VTTDTTLPLP SLNKDQEENK KDPLRIPTVF VYRQQQVLLE WIHQLMINDS
560 570 580 590 600
REFEIPELPD SLKNKISPYT HKKFDSTKLV EVLGIKKVDK LKLDSELKTE
610 620
LEREKIPDLE TLLKRGEQHS RGSPNNSNN
Length:629
Mass (Da):71,241
Last modified:November 1, 1997 - v1
Checksum:i58126164EE375643
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti149L → V in strain: DBVPG6044, SK1 and YPS128. 1
Natural varianti301S → L in strain: DBVPG1853. 1
Natural varianti349N → D in strain: DBVPG6044, SK1 and YPS128. 1
Natural varianti499G → D in strain: DBVPG1853. 1
Natural varianti518E → A in strain: DBVPG6044, SK1 and YPS128. 1
Natural varianti528T → A in strain: DBVPG1853. 1
Natural varianti585I → S in strain: DBVPG6763. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM296333 Genomic DNA Translation: CAL35984.1
AM296334 Genomic DNA Translation: CAL35983.1
AM296335 Genomic DNA Translation: CAL35982.1
AM296336 Genomic DNA Translation: CAL35981.1
AM296337 Genomic DNA Translation: CAL35980.1
AM296338 Genomic DNA Translation: CAL35979.1
AM296339 Genomic DNA Translation: CAL35978.1
AM296340 Genomic DNA Translation: CAL35977.1
AM296341 Genomic DNA Translation: CAL35976.1
AM296342 Genomic DNA Translation: CAL35975.1
AM296343 Genomic DNA Translation: CAL35974.1
AM296344 Genomic DNA Translation: CAL35973.1
AM296345 Genomic DNA Translation: CAL35972.1
Z49702 Genomic DNA Translation: CAA89742.1
BK006946 Genomic DNA Translation: DAA10003.1
PIRiS54567
RefSeqiNP_013824.1, NM_001182606.1

Genome annotation databases

EnsemblFungiiYMR106C; YMR106C; YMR106C
GeneIDi855132
KEGGisce:YMR106C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM296333 Genomic DNA Translation: CAL35984.1
AM296334 Genomic DNA Translation: CAL35983.1
AM296335 Genomic DNA Translation: CAL35982.1
AM296336 Genomic DNA Translation: CAL35981.1
AM296337 Genomic DNA Translation: CAL35980.1
AM296338 Genomic DNA Translation: CAL35979.1
AM296339 Genomic DNA Translation: CAL35978.1
AM296340 Genomic DNA Translation: CAL35977.1
AM296341 Genomic DNA Translation: CAL35976.1
AM296342 Genomic DNA Translation: CAL35975.1
AM296343 Genomic DNA Translation: CAL35974.1
AM296344 Genomic DNA Translation: CAL35973.1
AM296345 Genomic DNA Translation: CAL35972.1
Z49702 Genomic DNA Translation: CAA89742.1
BK006946 Genomic DNA Translation: DAA10003.1
PIRiS54567
RefSeqiNP_013824.1, NM_001182606.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5Y58X-ray2.80B/D/F2-629[»]
5Y59X-ray2.40B2-200[»]
ProteinModelPortaliQ04437
SMRiQ04437
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35281, 289 interactors
ComplexPortaliCPX-1732 Ku70:Ku80 complex
DIPiDIP-2757N
IntActiQ04437, 115 interactors
MINTiQ04437
STRINGi4932.YMR106C

Proteomic databases

MaxQBiQ04437
PaxDbiQ04437
PRIDEiQ04437

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR106C; YMR106C; YMR106C
GeneIDi855132
KEGGisce:YMR106C

Organism-specific databases

SGDiS000004712 YKU80

Phylogenomic databases

HOGENOMiHOG000066046
InParanoidiQ04437
KOiK10885
OMAiINVEGYP
OrthoDBiEOG092C1B2W

Enzyme and pathway databases

BioCyciYEAST:G3O-32803-MONOMER

Miscellaneous databases

PROiPR:Q04437

Family and domain databases

Gene3Di2.40.290.10, 1 hit
3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR006164 Ku70/Ku80_beta-barrel_dom
IPR024193 Ku80
IPR005161 Ku_N
IPR016194 SPOC-like_C_dom_sf
IPR036465 vWFA_dom_sf
PANTHERiPTHR12604:SF4 PTHR12604:SF4, 1 hit
PfamiView protein in Pfam
PF02735 Ku, 1 hit
PF03731 Ku_N, 1 hit
PIRSFiPIRSF016570 Ku80, 1 hit
SMARTiView protein in SMART
SM00559 Ku78, 1 hit
SUPFAMiSSF100939 SSF100939, 1 hit
SSF53300 SSF53300, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKU80_YEAST
AccessioniPrimary (citable) accession number: Q04437
Secondary accession number(s): D6VZS9
, Q0P737, Q0P738, Q0P741, Q0P749
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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