UniProtKB - Q04323 (UBXN1_HUMAN)
UBX domain-containing protein 1
UBXN1
Functioni
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- K48-linked polyubiquitin modification-dependent protein binding Source: ParkinsonsUK-UCL
- K6-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- proteasome regulatory particle binding Source: UniProtKB
- ubiquitin binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
GO - Biological processi
- double-strand break repair Source: Reactome
- negative regulation of ERAD pathway Source: ParkinsonsUK-UCL
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- negative regulation of protein K48-linked deubiquitination Source: ParkinsonsUK-UCL
- negative regulation of protein ubiquitination Source: UniProtKB
- negative regulation of ubiquitin-specific protease activity Source: ParkinsonsUK-UCL
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
- protein folding Source: Reactome
- viral process Source: UniProtKB-KW
Keywordsi
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | Q04323 |
Reactomei | R-HSA-532668, N-glycan trimming in the ER and Calnexin/Calreticulin cycle R-HSA-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
SIGNORi | Q04323 |
Protein family/group databases
MEROPSi | X36.001 |
Names & Taxonomyi
Protein namesi | Recommended name: UBX domain-containing protein 1Alternative name(s): SAPK substrate protein 1 UBA/UBX 33.3 kDa protein |
Gene namesi | Name:UBXN1 Synonyms:SAKS1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18402, UBXN1 |
MIMi | 616378, gene |
neXtProti | NX_Q04323 |
VEuPathDBi | HostDB:ENSG00000162191.13 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: ParkinsonsUK-UCL
Endoplasmic reticulum
- endoplasmic reticulum Source: ParkinsonsUK-UCL
- VCP-NPL4-UFD1 AAA ATPase complex Source: BHF-UCL
Nucleus
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- dendrite Source: Ensembl
- neuronal cell body Source: Ensembl
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 13 | M → T: Abolishes binding to 'Lys-6'-linked polyubiquitin chains and ability to inhibit E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. 1 Publication | 1 | |
Mutagenesisi | 219 | R → A: Does not affect binding to 'Lys-6'-linked polyubiquitin chains and ability to inhibit E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 51035 |
OpenTargetsi | ENSG00000162191 |
PharmGKBi | PA162408336 |
Miscellaneous databases
Pharosi | Q04323, Tbio |
Genetic variation databases
BioMutai | UBXN1 |
DMDMi | 30923268 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000211023 | 2 – 297 | UBX domain-containing protein 1Add BLAST | 296 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 199 | PhosphoserineCombined sources | 1 | |
Modified residuei | 200 | Phosphoserine; by MAPK121 Publication | 1 | |
Modified residuei | 207 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 229 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 270 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q04323 |
jPOSTi | Q04323 |
MassIVEi | Q04323 |
MaxQBi | Q04323 |
PaxDbi | Q04323 |
PeptideAtlasi | Q04323 |
PRIDEi | Q04323 |
ProteomicsDBi | 58240 [Q04323-1] 58241 [Q04323-2] |
PTM databases
iPTMneti | Q04323 |
MetOSitei | Q04323 |
PhosphoSitePlusi | Q04323 |
Expressioni
Gene expression databases
Bgeei | ENSG00000162191, Expressed in testis and 241 other tissues |
ExpressionAtlasi | Q04323, baseline and differential |
Genevisiblei | Q04323, HS |
Organism-specific databases
HPAi | ENSG00000162191, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with MAVS; this interaction prevents MAVS oligomerization and thus disrupts the RLR signaling pathway (PubMed:23545497).
Interacts with CUL1; this interaction inhibits CUL1-mediated degradation of NF-kappa-B inhibitors (PubMed:28152074).
Interacts with BIRC2/c-IAP1; this interaction prevents TNFalpha-stimulated RIP1 ubiquitination and subsequent NF-kappa-B activation (PubMed:25681446).
Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B.
Interacts with HOMER2 (By similarity).
Interacts directly with VCP.
Interacts with BRCA1 and BARD1; interaction takes place when BRCA1 is not autoubiquitinated but is strongly enhanced in the presence of autoubiquitinated BRCA1.
By similarity6 Publications(Microbial infection) Interacts (via C-terminal UBX domain) with nipah virus protein V.
1 PublicationBinary interactionsi
Hide detailsQ04323
Isoform 2 [Q04323-2]
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- K48-linked polyubiquitin modification-dependent protein binding Source: ParkinsonsUK-UCL
- K6-linked polyubiquitin modification-dependent protein binding Source: UniProtKB
- polyubiquitin modification-dependent protein binding Source: UniProtKB
- ubiquitin binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 119239, 171 interactors |
DIPi | DIP-29467N |
IntActi | Q04323, 73 interactors |
STRINGi | 9606.ENSP00000294119 |
Miscellaneous databases
RNActi | Q04323, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 42 | UBAPROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 211 – 293 | UBXPROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 43 – 297 | Interaction with BRCA11 PublicationAdd BLAST | 255 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 87 – 172 | Sequence analysisAdd BLAST | 86 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2689, Eukaryota |
GeneTreei | ENSGT00940000156457 |
InParanoidi | Q04323 |
OMAi | HTEVEYH |
PhylomeDBi | Q04323 |
TreeFami | TF313944 |
Family and domain databases
CDDi | cd14302, UBA_UBXN1, 1 hit |
InterProi | View protein in InterPro IPR015940, UBA IPR009060, UBA-like_sf IPR041923, UBA_UBXN1 IPR029071, Ubiquitin-like_domsf IPR001012, UBX_dom |
Pfami | View protein in Pfam PF00627, UBA, 1 hit PF00789, UBX, 1 hit |
SMARTi | View protein in SMART SM00165, UBA, 1 hit SM00166, UBX, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50030, UBA, 1 hit PS50033, UBX, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAELTALESL IEMGFPRGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE
60 70 80 90 100
PLETPLGHIL GREPTSSEQG GLEGSGSAAG EGKPALSEEE RQEQTKRMLE
110 120 130 140 150
LVAQKQRERE EREEREALER ERQRRRQGQE LSAARQRLQE DEMRRAAEER
160 170 180 190 200
RREKAEELAA RQRVREKIER DKAERAKKYG GSVGSQPPPV APEPGPVPSS
210 220 230 240 250
PSQEPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR LYVELHRGEE
260 270 280 290
LGGGQDPVQL LSGFPRRAFS EADMERPLQE LGLVPSAVLI VAKKCPS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WTZ5 | A0A087WTZ5_HUMAN | UBX domain-containing protein 1 | UBXN1 | 238 | Annotation score: | ||
E9PJ81 | E9PJ81_HUMAN | UBX domain-containing protein 1 | UBXN1 | 290 | Annotation score: | ||
E9PRQ7 | E9PRQ7_HUMAN | UBX domain-containing protein 1 | UBXN1 | 293 | Annotation score: | ||
E9PS08 | E9PS08_HUMAN | UBX domain-containing protein 1 | UBXN1 | 100 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 76 | G → A in AAA36396 (Ref. 1) Curated | 1 | |
Sequence conflicti | 148 | E → AE in AAA36396 (Ref. 1) Curated | 1 | |
Sequence conflicti | 154 | K → N in AAA36396 (Ref. 1) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057370 | 56 | L → F. Corresponds to variant dbSNP:rs11543359Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020367 | 283 – 297 | LVPSA…KKCPS → MAARLETRTRNWGSREACLG KGGMQREGAL in isoform 2. 1 PublicationAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68864 mRNA Translation: AAA36396.1 BC000902 mRNA Translation: AAH00902.1 BC001372 mRNA Translation: AAH01372.1 BC040129 mRNA Translation: AAH40129.1 |
CCDSi | CCDS66105.1 [Q04323-1] CCDS8029.1 [Q04323-2] |
PIRi | S27965 |
RefSeqi | NP_001273006.1, NM_001286077.1 [Q04323-1] NP_001273007.1, NM_001286078.1 NP_056937.2, NM_015853.4 [Q04323-2] XP_005274090.1, XM_005274033.4 [Q04323-2] XP_016873363.1, XM_017017874.1 [Q04323-1] |
Genome annotation databases
Ensembli | ENST00000294119; ENSP00000294119; ENSG00000162191 [Q04323-2] ENST00000301935; ENSP00000303991; ENSG00000162191 [Q04323-1] |
GeneIDi | 51035 |
KEGGi | hsa:51035 |
UCSCi | uc001nuj.5, human [Q04323-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68864 mRNA Translation: AAA36396.1 BC000902 mRNA Translation: AAH00902.1 BC001372 mRNA Translation: AAH01372.1 BC040129 mRNA Translation: AAH40129.1 |
CCDSi | CCDS66105.1 [Q04323-1] CCDS8029.1 [Q04323-2] |
PIRi | S27965 |
RefSeqi | NP_001273006.1, NM_001286077.1 [Q04323-1] NP_001273007.1, NM_001286078.1 NP_056937.2, NM_015853.4 [Q04323-2] XP_005274090.1, XM_005274033.4 [Q04323-2] XP_016873363.1, XM_017017874.1 [Q04323-1] |
3D structure databases
SMRi | Q04323 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119239, 171 interactors |
DIPi | DIP-29467N |
IntActi | Q04323, 73 interactors |
STRINGi | 9606.ENSP00000294119 |
Protein family/group databases
MEROPSi | X36.001 |
PTM databases
iPTMneti | Q04323 |
MetOSitei | Q04323 |
PhosphoSitePlusi | Q04323 |
Genetic variation databases
BioMutai | UBXN1 |
DMDMi | 30923268 |
Proteomic databases
EPDi | Q04323 |
jPOSTi | Q04323 |
MassIVEi | Q04323 |
MaxQBi | Q04323 |
PaxDbi | Q04323 |
PeptideAtlasi | Q04323 |
PRIDEi | Q04323 |
ProteomicsDBi | 58240 [Q04323-1] 58241 [Q04323-2] |
Protocols and materials databases
Antibodypediai | 1591, 118 antibodies |
DNASUi | 51035 |
Genome annotation databases
Ensembli | ENST00000294119; ENSP00000294119; ENSG00000162191 [Q04323-2] ENST00000301935; ENSP00000303991; ENSG00000162191 [Q04323-1] |
GeneIDi | 51035 |
KEGGi | hsa:51035 |
UCSCi | uc001nuj.5, human [Q04323-1] |
Organism-specific databases
CTDi | 51035 |
DisGeNETi | 51035 |
GeneCardsi | UBXN1 |
HGNCi | HGNC:18402, UBXN1 |
HPAi | ENSG00000162191, Low tissue specificity |
MIMi | 616378, gene |
neXtProti | NX_Q04323 |
OpenTargetsi | ENSG00000162191 |
PharmGKBi | PA162408336 |
VEuPathDBi | HostDB:ENSG00000162191.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2689, Eukaryota |
GeneTreei | ENSGT00940000156457 |
InParanoidi | Q04323 |
OMAi | HTEVEYH |
PhylomeDBi | Q04323 |
TreeFami | TF313944 |
Enzyme and pathway databases
PathwayCommonsi | Q04323 |
Reactomei | R-HSA-532668, N-glycan trimming in the ER and Calnexin/Calreticulin cycle R-HSA-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
SIGNORi | Q04323 |
Miscellaneous databases
BioGRID-ORCSi | 51035, 15 hits in 999 CRISPR screens |
ChiTaRSi | UBXN1, human |
GenomeRNAii | 51035 |
Pharosi | Q04323, Tbio |
PROi | PR:Q04323 |
RNActi | Q04323, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000162191, Expressed in testis and 241 other tissues |
ExpressionAtlasi | Q04323, baseline and differential |
Genevisiblei | Q04323, HS |
Family and domain databases
CDDi | cd14302, UBA_UBXN1, 1 hit |
InterProi | View protein in InterPro IPR015940, UBA IPR009060, UBA-like_sf IPR041923, UBA_UBXN1 IPR029071, Ubiquitin-like_domsf IPR001012, UBX_dom |
Pfami | View protein in Pfam PF00627, UBA, 1 hit PF00789, UBX, 1 hit |
SMARTi | View protein in SMART SM00165, UBA, 1 hit SM00166, UBX, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50030, UBA, 1 hit PS50033, UBX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBXN1_HUMAN | |
Accessioni | Q04323Primary (citable) accession number: Q04323 Secondary accession number(s): Q9BV93, Q9BVV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 16, 2003 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot