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Protein

Crossover junction endonuclease MUS81

Gene

MUS81

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with MMS4 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks with regressed leading strands and nicked Holliday junctions. Cleavage probably occurs approximately half a helical turn upstream of the free 5'-end in these structures. May be required in mitosis for the processing of stalled replication fork intermediates arising spontaneously or subsequent to treatment with DNA damaging agents such as methylmethane sulfonate (MMS), camptothecin (CPT) or UV. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI). This involves consecutive cleavage of D-loops and nicked Holliday junctions leading to sister chromatid crossover. In contrast to MSH4-MSH5 dependent crossover, double Holliday junctions do not seem to be involved. Spore formation and viability are severely impaired in deletion strains.11 Publications

Miscellaneous

Two distinct classes of meiotic crossovers have been demonstrated in budding yeast. Class I crossovers exhibit crossover interference and require MSH4 and MSH5 for their resolution, while class II crossovers exhibit no crossover interference and require MUS81 and MMS4. While class I crossovers represent the majority of crossovers in S.cerevisiae, they are virtually absent in S.pombe, which lacks orthologs of MSH4 and MSH5.
Present with 300 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Kineticsi

  1. KM=31.1 nM for a nicked Holliday junction1 Publication
  2. KM=6.84 nM for a regressed leading strand replication fork1 Publication
  3. KM=4.8 nM for for a 3'-flap structure1 Publication
  4. KM=3.45 nM for a nicked duplex1 Publication
  5. KM=14.0 nM for a regressed lagging strand replication fork1 Publication
  6. KM=245 nM for a Y structure1 Publication
  7. KM=173 nM for a double flap structure1 Publication
  1. Vmax=55.6 nmol/min/ng enzyme with a nicked Holliday junction as substrate1 Publication
  2. Vmax=31.3 nmol/min/ng enzyme with a regressed leading strand replication fork as substrate1 Publication
  3. Vmax=24.4 nmol/min/ng enzyme with a 3'-flap structure as substrate1 Publication
  4. Vmax=2.21 nmol/min/ng enzyme with a nicked duplex as substrate1 Publication
  5. Vmax=0.832 nmol/min/ng enzyme with a regressed lagging strand replication fork as subsystrate1 Publication
  6. Vmax=0.0468 nmol/min/ng enzyme with a Y structure as substrate1 Publication
  7. Vmax=0.0879 nmol/min/ng enzyme with a double flap structure as substrate1 Publication

pH dependencei

Optimum pH is 8.0 for the cleavage of a 3'-flap structure.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei415Curated1

GO - Molecular functioni

  • 3'-flap endonuclease activity Source: GO_Central
  • DNA binding Source: InterPro
  • enzyme inhibitor activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • DNA repair Source: SGD
  • DNA topological change Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • intra-S DNA damage checkpoint Source: GO_Central
  • mitotic G2 DNA damage checkpoint Source: GO_Central
  • negative regulation of helicase activity Source: SGD
  • reciprocal meiotic recombination Source: SGD
  • replication fork processing Source: GO_Central
  • resolution of meiotic recombination intermediates Source: SGD

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair, Meiosis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29934-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Alternative name(s):
MMS and UV-sensitive protein 81
Gene namesi
Name:MUS81
Synonyms:SLX3
Ordered Locus Names:YDR386W
ORF Names:D9509.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR386W
SGDiS000002794 MUS81

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi414 – 415DD → AA in allele MUS81-DD; abrogates endonuclease activity. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988611 – 632Crossover junction endonuclease MUS81Add BLAST632

Proteomic databases

MaxQBiQ04149
PaxDbiQ04149
PRIDEiQ04149

PTM databases

iPTMnetiQ04149

Interactioni

Subunit structurei

Interacts with MMS4 and RAD54.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MMS4P382575EBI-33508,EBI-21547

Protein-protein interaction databases

BioGridi32447, 205 interactors
ComplexPortaliCPX-1670 Deoxyribonuclease complex MUS81-MMS4
DIPiDIP-1009N
IntActiQ04149, 59 interactors
MINTiQ04149
STRINGi4932.YDR386W

Structurei

3D structure databases

ProteinModelPortaliQ04149
SMRiQ04149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini351 – 448ERCC4Add BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni527 – 632Interaction with MMS4Add BLAST106

Sequence similaritiesi

Belongs to the XPF family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005498
HOGENOMiHOG000248281
InParanoidiQ04149
KOiK08991
OMAiKCGRLQR
OrthoDBiEOG092C1AVP

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.10.150.110, 1 hit
InterProiView protein in InterPro
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR006166 ERCC4_domain
IPR011335 Restrct_endonuc-II-like
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
SMARTiView protein in SMART
SM00891 ERCC4, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52980 SSF52980, 1 hit

Sequencei

Sequence statusi: Complete.

Q04149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSSNLKDL YIEWLQELVD GLTPKQEQLK IAYEKAKRNL QNAEGSFYYP
60 70 80 90 100
TDLKKVKGIG NTIIKRLDTK LRNYCKIHHI SPVEAPSLTQ TSSTRPPKRT
110 120 130 140 150
TTALRSIVNS CENDKNEAPE EKGTKKRKTR KYIPKKRSGG YAILLSLLEL
160 170 180 190 200
NAIPRGVSKE QIIEVAGKYS DHCMTPNFST KEFYGAWSSI AALKKHSLVL
210 220 230 240 250
EEGRPKRYSL TEEGVELTKS LKTADGISFP KENEEPNEYS VTRNESSEFT
260 270 280 290 300
ANLTDLRGEY GKEEEPCDIN NTSFMLDITF QDLSTPQRLQ NNVFKNDRLN
310 320 330 340 350
SQTNISSHKL EEVSDDQTVP DSALKAKSTI KRRRYNGVSY ELWCSGDFEV
360 370 380 390 400
FPIIDHREIK SQSDREFFSR AFERKGMKSE IRQLALGDII WVAKNKNTGL
410 420 430 440 450
QCVLNTIVER KRLDDLALSI RDNRFMEQKN RLEKSGCEHK YYLIEETMSG
460 470 480 490 500
NIGNMNEALK TALWVILVYY KFSMIRTCNS DETVEKIHAL HTVISHHYSQ
510 520 530 540 550
KDLIVIFPSD LKSKDDYKKV LLQFRREFER KGGIECCHNL ECFQELMGKG
560 570 580 590 600
DLKTVGELTI HVLMLVKGIS LEKAVAIQEI FPTLNKILMA YKTCSSEEEA
610 620 630
KLLMFNVLGD APGAKKITKS LSEKIYDAFG KL
Length:632
Mass (Da):72,309
Last modified:November 1, 1996 - v1
Checksum:iF73FA3856C5F87F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32274 Genomic DNA Translation: AAB64828.1
BK006938 Genomic DNA Translation: DAA12230.1
PIRiS69670
RefSeqiNP_010674.3, NM_001180694.3

Genome annotation databases

EnsemblFungiiYDR386W; YDR386W; YDR386W
GeneIDi851994
KEGGisce:YDR386W

Similar proteinsi

Entry informationi

Entry nameiMUS81_YEAST
AccessioniPrimary (citable) accession number: Q04149
Secondary accession number(s): D6VT20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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