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Protein

Kynurenine formamidase

Gene

BNA7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.UniRule annotation1 Publication

Catalytic activityi

N-formyl-L-kynurenine + H2O = formate + L-kynurenine.UniRule annotation1 Publication

Pathwayi: L-tryptophan degradation via kynurenine pathway

This protein is involved in step 2 of the subpathway that synthesizes L-kynurenine from L-tryptophan.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Kynurenine formamidase (BNA7)
This subpathway is part of the pathway L-tryptophan degradation via kynurenine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-kynurenine from L-tryptophan, the pathway L-tryptophan degradation via kynurenine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei110NucleophileUniRule annotation1
Active sitei211UniRule annotation1
Active sitei243UniRule annotation1

GO - Molecular functioni

  • arylformamidase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTryptophan catabolism

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-17
BRENDAi3.5.1.9 984
UniPathwayi
UPA00333;UER00454

Protein family/group databases

ESTHERiyeast-YDR428C Kynurenine-formamidase

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine formamidaseUniRule annotation (EC:3.5.1.9UniRule annotation)
Short name:
KFAUniRule annotation
Short name:
KFaseUniRule annotation
Alternative name(s):
ArylformamidaseUniRule annotation
Biosynthesis of nicotinic acid protein 7
N-formylkynurenine formamidaseUniRule annotation
Short name:
FKFUniRule annotation
Gene namesi
Name:BNA7UniRule annotation
Ordered Locus Names:YDR428C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR428C
SGDiS000002836 BNA7

Pathology & Biotechi

Disruption phenotypei

Cells exhibit slow growth in the absence of nicotinate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002346601 – 261Kynurenine formamidaseAdd BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ04066
PaxDbiQ04066
PRIDEiQ04066

PTM databases

iPTMnetiQ04066

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi32486, 30 interactors
DIPiDIP-4139N
IntActiQ04066, 1 interactor
STRINGi4932.YDR428C

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ04066
SMRiQ04066
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04066

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 40HGGXW5

Domaini

The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.1 Publication

Sequence similaritiesi

Belongs to the kynurenine formamidase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000065929
InParanoidiQ04066
KOiK14263
OMAiHSCSAHM
OrthoDBiEOG092C48GX

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

Q04066-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNTVRAISP DITLFNKTLT FQEISQNTRE AVIYIHGGAW NDPENTPNDF
60 70 80 90 100
NQLANTIKSM DTESTVCQYS IEYRLSPEIT NPRNLYDAVS NITRLVKEKG
110 120 130 140 150
LTNINMVGHS VGATFIWQIL AALKDPQEKM SEAQLQMLGL LQIVKRVFLL
160 170 180 190 200
DGIYSLKELL VEYPEYDCFT RLAFPDGIQM YEEEPSRVMP YVKKALSRFS
210 220 230 240 250
IDMHLVHSYS DELLTLRQTN CLISCLQDYQ LSFKLYLDDL GLHNDVYKNG
260
KVAKYIFDNI C
Length:261
Mass (Da):29,991
Last modified:November 1, 1996 - v1
Checksum:iF07FE52DD9D2EF92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA Translation: AAB64885.1
BK006938 Genomic DNA Translation: DAA12267.1
PIRiS69709
RefSeqiNP_010716.3, NM_001180736.3

Genome annotation databases

EnsemblFungiiYDR428C; YDR428C; YDR428C
GeneIDi852038
KEGGisce:YDR428C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA Translation: AAB64885.1
BK006938 Genomic DNA Translation: DAA12267.1
PIRiS69709
RefSeqiNP_010716.3, NM_001180736.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VKHX-ray1.85A/B1-261[»]
ProteinModelPortaliQ04066
SMRiQ04066
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32486, 30 interactors
DIPiDIP-4139N
IntActiQ04066, 1 interactor
STRINGi4932.YDR428C

Protein family/group databases

ESTHERiyeast-YDR428C Kynurenine-formamidase

PTM databases

iPTMnetiQ04066

Proteomic databases

MaxQBiQ04066
PaxDbiQ04066
PRIDEiQ04066

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR428C; YDR428C; YDR428C
GeneIDi852038
KEGGisce:YDR428C

Organism-specific databases

EuPathDBiFungiDB:YDR428C
SGDiS000002836 BNA7

Phylogenomic databases

HOGENOMiHOG000065929
InParanoidiQ04066
KOiK14263
OMAiHSCSAHM
OrthoDBiEOG092C48GX

Enzyme and pathway databases

UniPathwayi
UPA00333;UER00454

BioCyciYEAST:MONOMER3O-17
BRENDAi3.5.1.9 984

Miscellaneous databases

EvolutionaryTraceiQ04066
PROiPR:Q04066

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_03014 KFase, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013094 AB_hydrolase_3
IPR027519 KFase_ver/fungi-typ
PfamiView protein in Pfam
PF07859 Abhydrolase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKFA_YEAST
AccessioniPrimary (citable) accession number: Q04066
Secondary accession number(s): D6VT57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: October 10, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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