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Protein

DNA polymerase eta

Gene

RAD30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O6-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.34 Publications

Miscellaneous

Present with 1860 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30MagnesiumPROSITE-ProRule annotation1
Metal bindingi155MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication, DNA synthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29960-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase eta (EC:2.7.7.7)
Alternative name(s):
Radiation-sensitive protein 30
Gene namesi
Name:RAD30
Synonyms:DBH1
Ordered Locus Names:YDR419W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR419W
SGDiS000002827 RAD30

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30D → A: Abolishes DNA polymerase activity. 1 Publication1
Mutagenesisi34F → L: Alters translesion activity. 1 Publication1
Mutagenesisi39E → A: Abolishes DNA polymerase activity. 1 Publication1
Mutagenesisi64Y → F or A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi67R → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi155D → A: Abolishes DNA polymerase activity and increases UV-induced mutations. 1 Publication1
Mutagenesisi156E → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi279K → A: Decreases efficiency of nucleotide incorporation. 1 Publication1
Mutagenesisi627F → A: Abolishes POL30-binding; when associated with A-628. 1
Mutagenesisi628F → A: Abolishes POL30-binding; when associated with A-627. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686981 – 632DNA polymerase etaAdd BLAST632

Proteomic databases

MaxQBiQ04049
PaxDbiQ04049
PRIDEiQ04049

Expressioni

Inductioni

By UV radiation and heat shock. The mRNA is stabilized during stationary phase.2 Publications

Interactioni

Subunit structurei

Interacts with POL30. This interaction is essential for the polymerase eta function.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
POL30P158737EBI-36214,EBI-12993

Protein-protein interaction databases

BioGridi32477, 138 interactors
DIPiDIP-6500N
IntActiQ04049, 19 interactors
MINTiQ04049
STRINGi4932.YDR419W

Structurei

Secondary structure

1632
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ04049
SMRiQ04049
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ04049

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 309UmuCPROSITE-ProRule annotationAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni625 – 632POL30-binding8

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

HOGENOMiHOG000065930
InParanoidiQ04049
KOiK03509
OMAiFGEESIW
OrthoDBiEOG092C18P5

Family and domain databases

Gene3Di3.30.1490.100, 1 hit
InterProiView protein in InterPro
IPR017061 DNA_pol_eta/kappa/iota/IV
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PIRSFiPIRSF036603 DPol_eta, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit

Sequencei

Sequence statusi: Complete.

Q04049-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKFTWKELI QLGSPSKAYE SSLACIAHID MNAFFAQVEQ MRCGLSKEDP
60 70 80 90 100
VVCVQWNSII AVSYAARKYG ISRMDTIQEA LKKCSNLIPI HTAVFKKGED
110 120 130 140 150
FWQYHDGCGS WVQDPAKQIS VEDHKVSLEP YRRESRKALK IFKSACDLVE
160 170 180 190 200
RASIDEVFLD LGRICFNMLM FDNEYELTGD LKLKDALSNI REAFIGGNYD
210 220 230 240 250
INSHLPLIPE KIKSLKFEGD VFNPEGRDLI TDWDDVILAL GSQVCKGIRD
260 270 280 290 300
SIKDILGYTT SCGLSSTKNV CKLASNYKKP DAQTIVKNDC LLDFLDCGKF
310 320 330 340 350
EITSFWTLGG VLGKELIDVL DLPHENSIKH IRETWPDNAG QLKEFLDAKV
360 370 380 390 400
KQSDYDRSTS NIDPLKTADL AEKLFKLSRG RYGLPLSSRP VVKSMMSNKN
410 420 430 440 450
LRGKSCNSIV DCISWLEVFC AELTSRIQDL EQEYNKIVIP RTVSISLKTK
460 470 480 490 500
SYEVYRKSGP VAYKGINFQS HELLKVGIKF VTDLDIKGKN KSYYPLTKLS
510 520 530 540 550
MTITNFDIID LQKTVVDMFG NQVHTFKSSA GKEDEEKTTS SKADEKTPKL
560 570 580 590 600
ECCKYQVTFT DQKALQEHAD YHLALKLSEG LNGAEESSKN LSFGEKRLLF
610 620 630
SRKRPNSQHT ATPQKKQVTS SKNILSFFTR KK
Length:632
Mass (Da):71,515
Last modified:November 1, 1996 - v1
Checksum:iCFB1A9FBC8AFE39B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA Translation: AAB64856.1
BK006938 Genomic DNA Translation: DAA12259.1
PIRiS69702
RefSeqiNP_010707.3, NM_001180727.3

Genome annotation databases

EnsemblFungiiYDR419W; YDR419W; YDR419W
GeneIDi852028
KEGGisce:YDR419W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33007 Genomic DNA Translation: AAB64856.1
BK006938 Genomic DNA Translation: DAA12259.1
PIRiS69702
RefSeqiNP_010707.3, NM_001180727.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JIHX-ray2.25A/B1-513[»]
2R8JX-ray3.10A/B1-531[»]
2R8KX-ray3.30A/B1-531[»]
2WTFX-ray2.50A/B1-513[»]
2XGPX-ray2.70A/B1-513[»]
2XGQX-ray2.70A/B1-513[»]
3MFHX-ray2.00A1-513[»]
3MFIX-ray1.76A1-513[»]
3OHAX-ray2.00A1-513[»]
3OHBX-ray2.00A1-513[»]
5VTPX-ray2.80A1-528[»]
ProteinModelPortaliQ04049
SMRiQ04049
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32477, 138 interactors
DIPiDIP-6500N
IntActiQ04049, 19 interactors
MINTiQ04049
STRINGi4932.YDR419W

Proteomic databases

MaxQBiQ04049
PaxDbiQ04049
PRIDEiQ04049

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR419W; YDR419W; YDR419W
GeneIDi852028
KEGGisce:YDR419W

Organism-specific databases

EuPathDBiFungiDB:YDR419W
SGDiS000002827 RAD30

Phylogenomic databases

HOGENOMiHOG000065930
InParanoidiQ04049
KOiK03509
OMAiFGEESIW
OrthoDBiEOG092C18P5

Enzyme and pathway databases

BioCyciYEAST:G3O-29960-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ04049
PROiPR:Q04049

Family and domain databases

Gene3Di3.30.1490.100, 1 hit
InterProiView protein in InterPro
IPR017061 DNA_pol_eta/kappa/iota/IV
IPR036775 DNA_pol_Y-fam_lit_finger_sf
IPR017961 DNA_pol_Y-fam_little_finger
IPR001126 UmuC
PfamiView protein in Pfam
PF00817 IMS, 1 hit
PF11799 IMS_C, 1 hit
PIRSFiPIRSF036603 DPol_eta, 1 hit
SUPFAMiSSF100879 SSF100879, 1 hit
PROSITEiView protein in PROSITE
PS50173 UMUC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPOLH_YEAST
AccessioniPrimary (citable) accession number: Q04049
Secondary accession number(s): D6VT49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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