UniProtKB - Q04049 (POLH_YEAST)
DNA polymerase eta
RAD30
Functioni
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O6-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.
34 PublicationsMiscellaneous
Catalytic activityi
- EC:2.7.7.7
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 30 | MagnesiumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 155 | MagnesiumPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 545 – 580 | UBZ3-typePROSITE-ProRule annotationAdd BLAST | 36 |
GO - Molecular functioni
- damaged DNA binding Source: InterPro
- DNA-directed DNA polymerase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- chromosome segregation Source: SGD
- DNA replication Source: UniProtKB-KW
- error-free translesion synthesis Source: SGD
- error-prone translesion synthesis Source: SGD
- mitotic sister chromatid cohesion Source: SGD
- response to radiation Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication, DNA synthesis |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-110320, Translesion Synthesis by POLH |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RAD30 Synonyms:DBH1 Ordered Locus Names:YDR419W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002827, RAD30 |
VEuPathDBi | FungiDB:YDR419W |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- replication fork Source: SGD
- site of double-strand break Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 30 | D → A: Abolishes DNA polymerase activity. 1 Publication | 1 | |
Mutagenesisi | 34 | F → L: Alters translesion activity. 1 Publication | 1 | |
Mutagenesisi | 39 | E → A: Abolishes DNA polymerase activity. 1 Publication | 1 | |
Mutagenesisi | 64 | Y → F or A: Decreases efficiency of nucleotide incorporation. 1 Publication | 1 | |
Mutagenesisi | 67 | R → A: Decreases efficiency of nucleotide incorporation. 1 Publication | 1 | |
Mutagenesisi | 155 | D → A: Abolishes DNA polymerase activity and increases UV-induced mutations. 1 Publication | 1 | |
Mutagenesisi | 156 | E → A: Decreases efficiency of nucleotide incorporation. 1 Publication | 1 | |
Mutagenesisi | 279 | K → A: Decreases efficiency of nucleotide incorporation. 1 Publication | 1 | |
Mutagenesisi | 627 | F → A: Abolishes POL30-binding; when associated with A-628. | 1 | |
Mutagenesisi | 628 | F → A: Abolishes POL30-binding; when associated with A-627. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000268698 | 1 – 632 | DNA polymerase etaAdd BLAST | 632 |
Proteomic databases
MaxQBi | Q04049 |
PaxDbi | Q04049 |
PRIDEi | Q04049 |
PTM databases
iPTMneti | Q04049 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Interacts with POL30. This interaction is essential for the polymerase eta function.
1 PublicationBinary interactionsi
Q04049
With | #Exp. | IntAct |
---|---|---|
POL30 [P15873] | 7 | EBI-36214,EBI-12993 |
Protein-protein interaction databases
BioGRIDi | 32477, 149 interactors |
DIPi | DIP-6500N |
IntActi | Q04049, 19 interactors |
MINTi | Q04049 |
STRINGi | 4932.YDR419W |
Miscellaneous databases
RNActi | Q04049, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q04049 |
SMRi | Q04049 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q04049 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 309 | UmuCPROSITE-ProRule annotationAdd BLAST | 284 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 598 – 632 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 625 – 632 | POL30-binding | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 604 – 626 | Polar residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 545 – 580 | UBZ3-typePROSITE-ProRule annotationAdd BLAST | 36 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2095, Eukaryota |
GeneTreei | ENSGT00940000157048 |
HOGENOMi | CLU_012348_7_3_1 |
InParanoidi | Q04049 |
OMAi | SACNKPN |
Family and domain databases
DisProti | DP02736 |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR043128, Rev_trsase/Diguanyl_cyclase IPR041298, UBZ3 IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit PS51907, ZF_UBZ3, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKFTWKELI QLGSPSKAYE SSLACIAHID MNAFFAQVEQ MRCGLSKEDP
60 70 80 90 100
VVCVQWNSII AVSYAARKYG ISRMDTIQEA LKKCSNLIPI HTAVFKKGED
110 120 130 140 150
FWQYHDGCGS WVQDPAKQIS VEDHKVSLEP YRRESRKALK IFKSACDLVE
160 170 180 190 200
RASIDEVFLD LGRICFNMLM FDNEYELTGD LKLKDALSNI REAFIGGNYD
210 220 230 240 250
INSHLPLIPE KIKSLKFEGD VFNPEGRDLI TDWDDVILAL GSQVCKGIRD
260 270 280 290 300
SIKDILGYTT SCGLSSTKNV CKLASNYKKP DAQTIVKNDC LLDFLDCGKF
310 320 330 340 350
EITSFWTLGG VLGKELIDVL DLPHENSIKH IRETWPDNAG QLKEFLDAKV
360 370 380 390 400
KQSDYDRSTS NIDPLKTADL AEKLFKLSRG RYGLPLSSRP VVKSMMSNKN
410 420 430 440 450
LRGKSCNSIV DCISWLEVFC AELTSRIQDL EQEYNKIVIP RTVSISLKTK
460 470 480 490 500
SYEVYRKSGP VAYKGINFQS HELLKVGIKF VTDLDIKGKN KSYYPLTKLS
510 520 530 540 550
MTITNFDIID LQKTVVDMFG NQVHTFKSSA GKEDEEKTTS SKADEKTPKL
560 570 580 590 600
ECCKYQVTFT DQKALQEHAD YHLALKLSEG LNGAEESSKN LSFGEKRLLF
610 620 630
SRKRPNSQHT ATPQKKQVTS SKNILSFFTR KK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33007 Genomic DNA Translation: AAB64856.1 BK006938 Genomic DNA Translation: DAA12259.1 |
PIRi | S69702 |
RefSeqi | NP_010707.3, NM_001180727.3 |
Genome annotation databases
EnsemblFungii | YDR419W_mRNA; YDR419W; YDR419W |
GeneIDi | 852028 |
KEGGi | sce:YDR419W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33007 Genomic DNA Translation: AAB64856.1 BK006938 Genomic DNA Translation: DAA12259.1 |
PIRi | S69702 |
RefSeqi | NP_010707.3, NM_001180727.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JIH | X-ray | 2.25 | A/B | 1-513 | [»] | |
2R8J | X-ray | 3.10 | A/B | 1-531 | [»] | |
2R8K | X-ray | 3.30 | A/B | 1-531 | [»] | |
2WTF | X-ray | 2.50 | A/B | 1-513 | [»] | |
2XGP | X-ray | 2.70 | A/B | 1-513 | [»] | |
2XGQ | X-ray | 2.70 | A/B | 1-513 | [»] | |
3MFH | X-ray | 2.00 | A | 1-513 | [»] | |
3MFI | X-ray | 1.76 | A | 1-513 | [»] | |
3OHA | X-ray | 2.00 | A | 1-513 | [»] | |
3OHB | X-ray | 2.00 | A | 1-513 | [»] | |
5VTP | X-ray | 2.80 | A | 1-528 | [»] | |
AlphaFoldDBi | Q04049 | |||||
SMRi | Q04049 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32477, 149 interactors |
DIPi | DIP-6500N |
IntActi | Q04049, 19 interactors |
MINTi | Q04049 |
STRINGi | 4932.YDR419W |
PTM databases
iPTMneti | Q04049 |
Proteomic databases
MaxQBi | Q04049 |
PaxDbi | Q04049 |
PRIDEi | Q04049 |
Genome annotation databases
EnsemblFungii | YDR419W_mRNA; YDR419W; YDR419W |
GeneIDi | 852028 |
KEGGi | sce:YDR419W |
Organism-specific databases
SGDi | S000002827, RAD30 |
VEuPathDBi | FungiDB:YDR419W |
Phylogenomic databases
eggNOGi | KOG2095, Eukaryota |
GeneTreei | ENSGT00940000157048 |
HOGENOMi | CLU_012348_7_3_1 |
InParanoidi | Q04049 |
OMAi | SACNKPN |
Enzyme and pathway databases
Reactomei | R-SCE-110320, Translesion Synthesis by POLH |
Miscellaneous databases
EvolutionaryTracei | Q04049 |
PROi | PR:Q04049 |
RNActi | Q04049, protein |
Family and domain databases
DisProti | DP02736 |
Gene3Di | 3.30.1490.100, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR036775, DNA_pol_Y-fam_lit_finger_sf IPR017961, DNA_pol_Y-fam_little_finger IPR043128, Rev_trsase/Diguanyl_cyclase IPR041298, UBZ3 IPR001126, UmuC |
Pfami | View protein in Pfam PF00817, IMS, 1 hit PF11799, IMS_C, 1 hit |
SUPFAMi | SSF100879, SSF100879, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS50173, UMUC, 1 hit PS51907, ZF_UBZ3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POLH_YEAST | |
Accessioni | Q04049Primary (citable) accession number: Q04049 Secondary accession number(s): D6VT49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 12, 2006 |
Last sequence update: | November 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families