UniProtKB - Q03C44 (PAGL1_LACP3)
6-phospho-alpha-glucosidase 1
simA
Functioni
Is probably involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including the five isomeric derivatives of sucrose, i.e. trehalulose-6'-phosphate, turanose-6'-phosphate, maltulose-6'-phosphate, leucrose-6'-phosphate, and palatinose-6'-phosphate, but is not active on sucrose-6-phosphate. Can also hydrolyze maltose-6'-phosphate and methyl-alpha-glucose-6-phosphate, and poorly, trehalose-6-phosphate. Fails to hydrolyze beta-O-linked phosphorylated disaccharides such as cellobiose-6'-phosphate and gentiobiose-6'-phosphate. Does not seem to be involved in maltose catabolism.
1 PublicationCatalytic activityi
- EC:3.2.1.1221 Publication
Cofactori
Protein has several cofactor binding sites:- Mn2+1 Publication, Co2+1 Publication, Ni2+1 Publication, Fe2+1 Publication, Mg2+1 PublicationNote: Binds 1 divalent metal ion per subunit. Mn2+ is the most efficient metal, but can also use Co2+, Ni2+ and Fe2+, and to a lesser extent Mg2+. Cannot use Zn2+.1 Publication
- NAD+1 PublicationNote: Binds 1 NAD+ per subunit.1 Publication
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | SubstrateBy similarity | 1 | |
Sitei | 111 | Increases basicity of active site TyrBy similarity | 1 | |
Binding sitei | 149 | SubstrateBy similarity | 1 | |
Metal bindingi | 171 | ManganeseBy similarity | 1 | |
Active sitei | 172 | Proton donorCurated | 1 | |
Metal bindingi | 202 | ManganeseBy similarity | 1 | |
Active sitei | 265 | Proton acceptorCurated | 1 | |
Binding sitei | 285 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 6 – 72 | NADBy similarityAdd BLAST | 67 |
GO - Molecular functioni
- maltose-6'-phosphate glucosidase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Cobalt, Iron, Manganese, Metal-binding, NAD, Nickel |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:simA Ordered Locus Names:LSEI_0369 |
Organismi | Lacticaseibacillus paracasei (strain ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL B-441) (Lactobacillus paracasei) |
Taxonomic identifieri | 321967 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lacticaseibacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000389204 | 1 – 442 | 6-phospho-alpha-glucosidase 1Add BLAST | 442 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer. May also form homotetramer.
1 PublicationProtein-protein interaction databases
STRINGi | 321967.LSEI_0369 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_045951_2_0_9 |
OMAi | METYSPD |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDDRKFSVLI AGGGSTYTPG IVLTLLDHIQ KFPLRKLKFY DIDGERQQRV
60 70 80 90 100
ADACEILVKE RAPEVEFLAT TDPEEAFTDV DFVMAQIRVG KYAMRSLDEK
110 120 130 140 150
IPLKHGVVGQ ETTGPGGIAY GLRSIPGVIG LVDYMEKYSP NAWMLNYSNP
160 170 180 190 200
AAIVAEATRR LRPHSRIINI CDMPIGIMDR MAQIVGLKDR NDLVFRYYGL
210 220 230 240 250
NHFGWWTDVR DKTGKDLMPA LKQYVAKNGY WLGDKDKDTE ASWVSTFKKA
260 270 280 290 300
ADVYALDPST LPNTYLKYYL YPKYVVEHSD PNYTRTDEVE AYREKHVFDE
310 320 330 340 350
CDRIIAAGTA ADTHFKSDDH ATYIVDLCTA IAYDTKQRML AIVPNDGAIE
360 370 380 390 400
NIDPEAMVEV PCLFGANGAE RLAMGKAATF QKGLITEQNC VEKLTVDAFE
410 420 430 440
QQSYTKLWEA MSLCKIVPDA SVAKEILDEM VVANKDYWPE LK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000423 Genomic DNA Translation: ABJ69228.1 |
RefSeqi | WP_011674086.1, NC_008526.1 YP_805670.1, NC_008526.1 |
Genome annotation databases
EnsemblBacteriai | ABJ69228; ABJ69228; LSEI_0369 |
KEGGi | lca:LSEI_0369 |
PATRICi | fig|321967.11.peg.390 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000423 Genomic DNA Translation: ABJ69228.1 |
RefSeqi | WP_011674086.1, NC_008526.1 YP_805670.1, NC_008526.1 |
3D structure databases
SMRi | Q03C44 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 321967.LSEI_0369 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Genome annotation databases
EnsemblBacteriai | ABJ69228; ABJ69228; LSEI_0369 |
KEGGi | lca:LSEI_0369 |
PATRICi | fig|321967.11.peg.390 |
Phylogenomic databases
HOGENOMi | CLU_045951_2_0_9 |
OMAi | METYSPD |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAGL1_LACP3 | |
Accessioni | Q03C44Primary (citable) accession number: Q03C44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 24, 2009 |
Last sequence update: | November 14, 2006 | |
Last modified: | February 23, 2022 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families