UniProtKB - Q03989 (ARI5A_HUMAN)
AT-rich interactive domain-containing protein 5A
ARID5A
Functioni
Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer-dependent gene expressison (PubMed:8649988).
May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (By similarity).
Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (PubMed:15941852).
As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (By similarity).
Stabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR; thereby competing with the mRNA-destabilizing functions of RC3H1 and endoribonuclease ZC3H12A (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- chromatin binding Source: Ensembl
- DNA binding Source: GDB
- estrogen receptor binding Source: UniProtKB
- identical protein binding Source: IntAct
- retinoid X receptor binding Source: UniProtKB
- RNA stem-loop binding Source: Ensembl
- sequence-specific DNA binding Source: NTNU_SB
- thyroid hormone receptor binding Source: UniProtKB
- transcription cis-regulatory region binding Source: GO_Central
- transcription corepressor activity Source: UniProtKB
- transcription factor binding Source: UniProtKB
GO - Biological processi
- cellular response to estrogen stimulus Source: UniProtKB
- cellular response to lipopolysaccharide Source: Ensembl
- chondrocyte differentiation Source: Ensembl
- innate immune response Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: GDB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of histone acetylation Source: Ensembl
- positive regulation of interferon-gamma production Source: Ensembl
- positive regulation of interleukin-17 production Source: Ensembl
- positive regulation of interleukin-6 production Source: Ensembl
- positive regulation of T-helper 17 type immune response Source: Ensembl
- positive regulation of T-helper 1 cell cytokine production Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: Ensembl
- positive regulation of tumor necrosis factor production Source: Ensembl
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor, RNA-binding |
Biological process | Immunity, Innate immunity, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q03989 |
SignaLinki | Q03989 |
Names & Taxonomyi
Protein namesi | Recommended name: AT-rich interactive domain-containing protein 5AShort name: ARID domain-containing protein 5A Alternative name(s): Modulator recognition factor 1 Short name: MRF-1 |
Gene namesi | Name:ARID5A Synonyms:MRF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17361, ARID5A |
MIMi | 611583, gene |
neXtProti | NX_Q03989 |
VEuPathDBi | HostDB:ENSG00000196843 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- transcription regulator complex Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10865 |
OpenTargetsi | ENSG00000196843 |
PharmGKBi | PA134937822 |
Miscellaneous databases
Pharosi | Q03989, Tbio |
Genetic variation databases
BioMutai | ARID5A |
DMDMi | 148840818 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000288930 | 1 – 594 | AT-rich interactive domain-containing protein 5AAdd BLAST | 594 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Cross-linki | 85 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 94 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 256 | PhosphoserineBy similarity | 1 | |
Modified residuei | 289 | PhosphoserineBy similarity | 1 | |
Modified residuei | 438 | PhosphoserineBy similarity | 1 | |
Modified residuei | 463 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q03989 |
MassIVEi | Q03989 |
MaxQBi | Q03989 |
PaxDbi | Q03989 |
PeptideAtlasi | Q03989 |
PRIDEi | Q03989 |
ProteomicsDBi | 58234 8111 |
PTM databases
iPTMneti | Q03989 |
PhosphoSitePlusi | Q03989 |
Expressioni
Gene expression databases
Bgeei | ENSG00000196843, Expressed in tibial artery and 227 other tissues |
ExpressionAtlasi | Q03989, baseline and differential |
Genevisiblei | Q03989, HS |
Organism-specific databases
HPAi | ENSG00000196843, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with SOX9 (By similarity).
Interacts with ESR1 (PubMed:15941852).
Interacts with RORC (By similarity).
By similarity1 PublicationBinary interactionsi
Q03989
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- estrogen receptor binding Source: UniProtKB
- identical protein binding Source: IntAct
- retinoid X receptor binding Source: UniProtKB
- thyroid hormone receptor binding Source: UniProtKB
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116074, 132 interactors |
IntActi | Q03989, 125 interactors |
MINTi | Q03989 |
STRINGi | 9606.ENSP00000350078 |
Miscellaneous databases
RNActi | Q03989, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 147 | ARIDPROSITE-ProRule annotationAdd BLAST | 93 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 300 | Interaction with SOX9By similarityAdd BLAST | 300 | |
Regioni | 1 – 56 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 146 – 223 | DisorderedSequence analysisAdd BLAST | 78 | |
Regioni | 281 – 331 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 426 – 454 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 146 – 193 | Basic and acidic residuesSequence analysisAdd BLAST | 48 | |
Compositional biasi | 300 – 314 | Polar residuesSequence analysisAdd BLAST | 15 |
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota |
GeneTreei | ENSGT00940000161253 |
InParanoidi | Q03989 |
OMAi | ECGAKSM |
OrthoDBi | 368297at2759 |
PhylomeDBi | Q03989 |
TreeFami | TF324725 |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR001606, ARID_dom IPR036431, ARID_dom_sf |
Pfami | View protein in Pfam PF01388, ARID, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit |
SUPFAMi | SSF46774, SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAPVKGNRK QSTEGDALDP PASPKPAGKQ NGIQNPISLE DSPEAGGERE
60 70 80 90 100
EEQEREEEQA FLVSLYKFMK ERHTPIERVP HLGFKQINLW KIYKAVEKLG
110 120 130 140 150
AYELVTGRRL WKNVYDELGG SPGSTSAATC TRRHYERLVL PYVRHLKGED
160 170 180 190 200
DKPLPTSKPR KQYKMAKENR GDDGATERPK KAKEERRMDQ MMPGKTKADA
210 220 230 240 250
ADPAPLPSQE PPRNSTEQQG LASGSSVSFV GASGCPEAYK RLLSSFYCKG
260 270 280 290 300
THGIMSPLAK KKLLAQVSKV EALQCQEEGC RHGAEPQASP AVHLPESPQS
310 320 330 340 350
PKGLTENSRH RLTPQEGLQA PGGSLREEAQ AGPCPAAPIF KGCFYTHPTE
360 370 380 390 400
VLKPVSQHPR DFFSRLKDGV LLGPPGKEGL SVKEPQLVWG GDANRPSAFH
410 420 430 440 450
KGGSRKGILY PKPKACWVSP MAKVPAESPT LPPTFPSSPG LGSKRSLEEE
460 470 480 490 500
GAAHSGKRLR AVSPFLKEAD AKKCGAKPAG SGLVSCLLGP ALGPVPPEAY
510 520 530 540 550
RGTMLHCPLN FTGTPGPLKG QAALPFSPLV IPAFPAHFLA TAGPSPMAAG
560 570 580 590
LMHFPPTSFD SALRHRLCPA SSAWHAPPVT TYAAPHFFHL NTKL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A669KBH0 | A0A669KBH0_HUMAN | AT-rich interactive domain-containi... | ARID5A | 327 | Annotation score: | ||
F6Q9D3 | F6Q9D3_HUMAN | AT-rich interactive domain-containi... | ARID5A | 58 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 392 | D → G in BC047390 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058969 | 1 – 68 | Missing in isoform 2. Add BLAST | 68 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M62324 mRNA Translation: AAA36325.1 Different initiation. AC013270 Genomic DNA No translation available. CH471207 Genomic DNA Translation: EAW71359.1 BC047390 mRNA No translation available. |
CCDSi | CCDS33251.1 [Q03989-1] CCDS82484.1 [Q03989-5] |
PIRi | S27962 |
RefSeqi | NP_001306021.1, NM_001319092.1 [Q03989-5] NP_997646.1, NM_212481.2 [Q03989-1] |
Genome annotation databases
Ensembli | ENST00000357485; ENSP00000350078; ENSG00000196843 ENST00000454558; ENSP00000400785; ENSG00000196843 [Q03989-5] |
GeneIDi | 10865 |
KEGGi | hsa:10865 |
MANE-Selecti | ENST00000357485.8; ENSP00000350078.3; NM_212481.3; NP_997646.1 |
UCSCi | uc002swe.4, human [Q03989-1] uc031ron.2, human |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M62324 mRNA Translation: AAA36325.1 Different initiation. AC013270 Genomic DNA No translation available. CH471207 Genomic DNA Translation: EAW71359.1 BC047390 mRNA No translation available. |
CCDSi | CCDS33251.1 [Q03989-1] CCDS82484.1 [Q03989-5] |
PIRi | S27962 |
RefSeqi | NP_001306021.1, NM_001319092.1 [Q03989-5] NP_997646.1, NM_212481.2 [Q03989-1] |
3D structure databases
SMRi | Q03989 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116074, 132 interactors |
IntActi | Q03989, 125 interactors |
MINTi | Q03989 |
STRINGi | 9606.ENSP00000350078 |
PTM databases
iPTMneti | Q03989 |
PhosphoSitePlusi | Q03989 |
Genetic variation databases
BioMutai | ARID5A |
DMDMi | 148840818 |
Proteomic databases
jPOSTi | Q03989 |
MassIVEi | Q03989 |
MaxQBi | Q03989 |
PaxDbi | Q03989 |
PeptideAtlasi | Q03989 |
PRIDEi | Q03989 |
ProteomicsDBi | 58234 8111 |
Protocols and materials databases
Antibodypediai | 1050, 218 antibodies from 18 providers |
DNASUi | 10865 |
Genome annotation databases
Ensembli | ENST00000357485; ENSP00000350078; ENSG00000196843 ENST00000454558; ENSP00000400785; ENSG00000196843 [Q03989-5] |
GeneIDi | 10865 |
KEGGi | hsa:10865 |
MANE-Selecti | ENST00000357485.8; ENSP00000350078.3; NM_212481.3; NP_997646.1 |
UCSCi | uc002swe.4, human [Q03989-1] uc031ron.2, human |
Organism-specific databases
CTDi | 10865 |
DisGeNETi | 10865 |
GeneCardsi | ARID5A |
HGNCi | HGNC:17361, ARID5A |
HPAi | ENSG00000196843, Low tissue specificity |
MIMi | 611583, gene |
neXtProti | NX_Q03989 |
OpenTargetsi | ENSG00000196843 |
PharmGKBi | PA134937822 |
VEuPathDBi | HostDB:ENSG00000196843 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota |
GeneTreei | ENSGT00940000161253 |
InParanoidi | Q03989 |
OMAi | ECGAKSM |
OrthoDBi | 368297at2759 |
PhylomeDBi | Q03989 |
TreeFami | TF324725 |
Enzyme and pathway databases
PathwayCommonsi | Q03989 |
SignaLinki | Q03989 |
Miscellaneous databases
BioGRID-ORCSi | 10865, 27 hits in 1071 CRISPR screens |
ChiTaRSi | ARID5A, human |
GenomeRNAii | 10865 |
Pharosi | Q03989, Tbio |
PROi | PR:Q03989 |
RNActi | Q03989, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196843, Expressed in tibial artery and 227 other tissues |
ExpressionAtlasi | Q03989, baseline and differential |
Genevisiblei | Q03989, HS |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR001606, ARID_dom IPR036431, ARID_dom_sf |
Pfami | View protein in Pfam PF01388, ARID, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit |
SUPFAMi | SSF46774, SSF46774, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ARI5A_HUMAN | |
Accessioni | Q03989Primary (citable) accession number: Q03989 Secondary accession number(s): C9J1Q0, Q6NX37 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | May 29, 2007 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot