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Protein

AT-rich interactive domain-containing protein 5A

Gene

ARID5A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer-dependent gene expressison (PubMed:8649988). May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (By similarity). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (PubMed:15941852). As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor, RNA-binding
Biological processImmunity, Innate immunity, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 5A
Short name:
ARID domain-containing protein 5A
Alternative name(s):
Modulator recognition factor 1
Short name:
MRF-1
Gene namesi
Name:ARID5A
Synonyms:MRF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000196843.15
HGNCiHGNC:17361 ARID5A
MIMi611583 gene
neXtProtiNX_Q03989

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000196843
PharmGKBiPA134937822

Polymorphism and mutation databases

BioMutaiARID5A
DMDMi148840818

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002889301 – 594AT-rich interactive domain-containing protein 5AAdd BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineBy similarity1
Cross-linki85Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki94Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei256PhosphoserineBy similarity1
Modified residuei289PhosphoserineBy similarity1
Modified residuei438PhosphoserineBy similarity1
Modified residuei463PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by MAPK14 on serine residues involving a TLR4 signaling pathway upon lipopolysaccharide (LPS) stimulation leading to its ubiquitination and proteasomal degradation.By similarity
Ubiquitinated leading to proteasomal degradation; involving WWP1 linked to MAPK14-mediated phosphorylation upon LPS stimulation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ03989
PaxDbiQ03989
PeptideAtlasiQ03989
PRIDEiQ03989
ProteomicsDBi58234

PTM databases

iPTMnetiQ03989
PhosphoSitePlusiQ03989

Expressioni

Gene expression databases

BgeeiENSG00000196843 Expressed in 217 organ(s), highest expression level in tibial artery
CleanExiHS_ARID5A
ExpressionAtlasiQ03989 baseline and differential
GenevisibleiQ03989 HS

Organism-specific databases

HPAiHPA002860
HPA023879

Interactioni

Subunit structurei

Interacts with SOX9 (By similarity). Interacts with ESR1 (PubMed:15941852). Interacts with RORC (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116074, 20 interactors
IntActiQ03989, 123 interactors
STRINGi9606.ENSP00000350078

Structurei

3D structure databases

ProteinModelPortaliQ03989
SMRiQ03989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 147ARIDPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 300Interaction with SOX9By similarityAdd BLAST300

Phylogenomic databases

eggNOGiKOG2744 Eukaryota
ENOG410Y2AP LUCA
GeneTreeiENSGT00900000140901
HOGENOMiHOG000034050
HOVERGENiHBG062210
InParanoidiQ03989
OMAiHFFHLNT
OrthoDBiEOG091G0E6N
PhylomeDBiQ03989
TreeFamiTF324725

Family and domain databases

Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
PfamiView protein in Pfam
PF01388 ARID, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SUPFAMiSSF46774 SSF46774, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q03989-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAPVKGNRK QSTEGDALDP PASPKPAGKQ NGIQNPISLE DSPEAGGERE
60 70 80 90 100
EEQEREEEQA FLVSLYKFMK ERHTPIERVP HLGFKQINLW KIYKAVEKLG
110 120 130 140 150
AYELVTGRRL WKNVYDELGG SPGSTSAATC TRRHYERLVL PYVRHLKGED
160 170 180 190 200
DKPLPTSKPR KQYKMAKENR GDDGATERPK KAKEERRMDQ MMPGKTKADA
210 220 230 240 250
ADPAPLPSQE PPRNSTEQQG LASGSSVSFV GASGCPEAYK RLLSSFYCKG
260 270 280 290 300
THGIMSPLAK KKLLAQVSKV EALQCQEEGC RHGAEPQASP AVHLPESPQS
310 320 330 340 350
PKGLTENSRH RLTPQEGLQA PGGSLREEAQ AGPCPAAPIF KGCFYTHPTE
360 370 380 390 400
VLKPVSQHPR DFFSRLKDGV LLGPPGKEGL SVKEPQLVWG GDANRPSAFH
410 420 430 440 450
KGGSRKGILY PKPKACWVSP MAKVPAESPT LPPTFPSSPG LGSKRSLEEE
460 470 480 490 500
GAAHSGKRLR AVSPFLKEAD AKKCGAKPAG SGLVSCLLGP ALGPVPPEAY
510 520 530 540 550
RGTMLHCPLN FTGTPGPLKG QAALPFSPLV IPAFPAHFLA TAGPSPMAAG
560 570 580 590
LMHFPPTSFD SALRHRLCPA SSAWHAPPVT TYAAPHFFHL NTKL
Length:594
Mass (Da):64,074
Last modified:May 29, 2007 - v2
Checksum:i89A494C4560416FF
GO
Isoform 2 (identifier: Q03989-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Show »
Length:526
Mass (Da):56,694
Checksum:i2CB14C05D57D1A35
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Q9D3F6Q9D3_HUMAN
AT-rich interactive domain-containi...
ARID5A
58Annotation score:

Sequence cautioni

The sequence AAA36325 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti392D → G in BC047390 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0589691 – 68Missing in isoform 2. Add BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62324 mRNA Translation: AAA36325.1 Different initiation.
AC013270 Genomic DNA No translation available.
CH471207 Genomic DNA Translation: EAW71359.1
BC047390 mRNA No translation available.
CCDSiCCDS33251.1 [Q03989-1]
CCDS82484.1 [Q03989-5]
PIRiS27962
RefSeqiNP_001306021.1, NM_001319092.1 [Q03989-5]
NP_997646.1, NM_212481.2 [Q03989-1]
UniGeneiHs.920

Genome annotation databases

EnsembliENST00000357485; ENSP00000350078; ENSG00000196843 [Q03989-1]
ENST00000454558; ENSP00000400785; ENSG00000196843 [Q03989-5]
GeneIDi10865
KEGGihsa:10865
UCSCiuc002swe.4 human [Q03989-1]
uc031ron.2 human

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62324 mRNA Translation: AAA36325.1 Different initiation.
AC013270 Genomic DNA No translation available.
CH471207 Genomic DNA Translation: EAW71359.1
BC047390 mRNA No translation available.
CCDSiCCDS33251.1 [Q03989-1]
CCDS82484.1 [Q03989-5]
PIRiS27962
RefSeqiNP_001306021.1, NM_001319092.1 [Q03989-5]
NP_997646.1, NM_212481.2 [Q03989-1]
UniGeneiHs.920

3D structure databases

ProteinModelPortaliQ03989
SMRiQ03989
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116074, 20 interactors
IntActiQ03989, 123 interactors
STRINGi9606.ENSP00000350078

PTM databases

iPTMnetiQ03989
PhosphoSitePlusiQ03989

Polymorphism and mutation databases

BioMutaiARID5A
DMDMi148840818

Proteomic databases

MaxQBiQ03989
PaxDbiQ03989
PeptideAtlasiQ03989
PRIDEiQ03989
ProteomicsDBi58234

Protocols and materials databases

DNASUi10865
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357485; ENSP00000350078; ENSG00000196843 [Q03989-1]
ENST00000454558; ENSP00000400785; ENSG00000196843 [Q03989-5]
GeneIDi10865
KEGGihsa:10865
UCSCiuc002swe.4 human [Q03989-1]
uc031ron.2 human

Organism-specific databases

CTDi10865
EuPathDBiHostDB:ENSG00000196843.15
GeneCardsiARID5A
HGNCiHGNC:17361 ARID5A
HPAiHPA002860
HPA023879
MIMi611583 gene
neXtProtiNX_Q03989
OpenTargetsiENSG00000196843
PharmGKBiPA134937822
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2744 Eukaryota
ENOG410Y2AP LUCA
GeneTreeiENSGT00900000140901
HOGENOMiHOG000034050
HOVERGENiHBG062210
InParanoidiQ03989
OMAiHFFHLNT
OrthoDBiEOG091G0E6N
PhylomeDBiQ03989
TreeFamiTF324725

Miscellaneous databases

ChiTaRSiARID5A human
GenomeRNAii10865
PROiPR:Q03989
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196843 Expressed in 217 organ(s), highest expression level in tibial artery
CleanExiHS_ARID5A
ExpressionAtlasiQ03989 baseline and differential
GenevisibleiQ03989 HS

Family and domain databases

Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
PfamiView protein in Pfam
PF01388 ARID, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SUPFAMiSSF46774 SSF46774, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARI5A_HUMAN
AccessioniPrimary (citable) accession number: Q03989
Secondary accession number(s): C9J1Q0, Q6NX37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: September 12, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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