UniProtKB - Q03963 (E2AK2_MOUSE)
Interferon-induced, double-stranded RNA-activated protein kinase
Eif2ak2
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 271 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 376 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 248 – 256 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- double-stranded RNA binding Source: MGI
- eukaryotic translation initiation factor 2alpha kinase activity Source: MGI
- identical protein binding Source: MGI
- non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
- protein kinase activity Source: MGI
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- activation of MAPKK activity Source: UniProtKB
- cellular response to amino acid starvation Source: MGI
- defense response to virus Source: UniProtKB-KW
- endoplasmic reticulum unfolded protein response Source: MGI
- innate immune response Source: UniProtKB-KW
- negative regulation of apoptotic process Source: MGI
- negative regulation of osteoblast proliferation Source: UniProtKB
- negative regulation of translation Source: UniProtKB
- negative regulation of viral genome replication Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of chemokine production Source: UniProtKB
- positive regulation of cytokine production Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
- positive regulation of stress-activated MAPK cascade Source: UniProtKB
- protein autophosphorylation Source: UniProtKB
- protein phosphorylation Source: MGI
- regulation of hematopoietic progenitor cell differentiation Source: UniProtKB
- regulation of hematopoietic stem cell differentiation Source: UniProtKB
- regulation of hematopoietic stem cell proliferation Source: UniProtKB
- regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
- response to interferon-alpha Source: UniProtKB
- response to lipopolysaccharide Source: MGI
- response to toxic substance Source: MGI
- response to virus Source: UniProtKB
- response to vitamin E Source: MGI
- translation Source: MGI
Keywordsi
Molecular function | Kinase, RNA-binding, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase |
Biological process | Antiviral defense, Immunity, Innate immunity, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1169408, ISG15 antiviral mechanism |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon-induced, double-stranded RNA-activated protein kinase (EC:2.7.11.1)Alternative name(s): Eukaryotic translation initiation factor 2-alpha kinase 2 Short name: eIF-2A protein kinase 2 Interferon-inducible RNA-dependent protein kinase P1/eIF-2A protein kinase Protein kinase RNA-activated Short name: PKR Short name: Protein kinase R1 Publication Serine/threonine-protein kinase TIK Tyrosine-protein kinase EIF2AK2 (EC:2.7.10.2) p68 kinase |
Gene namesi | Name:Eif2ak2 Synonyms:Pkr, Prkr, Tik |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1353449, Eif2ak2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
Cytosol
- cytosol Source: MGI
- cytosolic ribosome Source: GO_Central
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL1795121 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000085946 | 2 – 515 | Interferon-induced, double-stranded RNA-activated protein kinaseAdd BLAST | 514 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Cross-linki | 68 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity | ||
Modified residuei | 84 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 96 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Cross-linki | 154 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity | ||
Modified residuei | 157 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 233 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 268 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 409 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 414 | Phosphothreonine; by autocatalysisBy similarity | 1 | |
Modified residuei | 419 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q03963 |
jPOSTi | Q03963 |
MaxQBi | Q03963 |
PaxDbi | Q03963 |
PeptideAtlasi | Q03963 |
PRIDEi | Q03963 |
PTM databases
iPTMneti | Q03963 |
PhosphoSitePlusi | Q03963 |
SwissPalmi | Q03963 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000024079, Expressed in epithelium of small intestine and 275 other tissues |
Genevisiblei | Q03963, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with DNAJC3 and STRBP (By similarity).
Forms a complex with FANCA, FANCC, FANCG and HSP70 (By similarity).
Interacts with ADAR/ADAR1. The inactive form interacts with NCK1.
Interacts (via the kinase catalytic domain) with STAT3 (via SH2 domain), TRAF2 (C-terminus), TRAF5 (C-terminus) and TRAF6 (C-terminus).
Interacts with MAP2K6, TARBP2, NLRP1, NLRC4 and AIM2.
Interacts (via DRBM 1 domain) with DUS2L (via DRBM domain) (By similarity).
Interacts with DHX9 (via N-terminus) and this interaction is dependent upon activation of the kinase. The inactive form interacts with GSN.
Interacts with IKBKB/IKKB, NPM1, NLRP3 and IRS1.
By similarity7 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202376, 11 interactors |
DIPi | DIP-41411N |
IntActi | Q03963, 4 interactors |
STRINGi | 10090.ENSMUSP00000024884 |
Miscellaneous databases
RNActi | Q03963, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q03963 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q03963 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 76 | DRBM 1PROSITE-ProRule annotationAdd BLAST | 69 | |
Domaini | 95 – 162 | DRBM 2PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 242 – 504 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 263 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 241 – 515 | Interaction with TRAF5By similarityAdd BLAST | 275 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 183 – 187 | Poly-Ser | 5 | |
Compositional biasi | 241 – 247 | Asp/Glu-rich (acidic) | 7 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1033, Eukaryota |
GeneTreei | ENSGT00940000160736 |
HOGENOMi | CLU_023682_1_0_1 |
InParanoidi | Q03963 |
OMAi | LIQMEFC |
OrthoDBi | 1195366at2759 |
PhylomeDBi | Q03963 |
TreeFami | TF317576 |
Family and domain databases
CDDi | cd00048, DSRM, 2 hits |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MASDTPGFYM DKLNKYRQMH GVAITYKELS TSGPPHDRRF TFQVLIDEKE
60 70 80 90 100
FPEAKGRSKQ EARNAAAKLA VDILDNENKV DCHTSASEQG LFVGNYIGLV
110 120 130 140 150
NSFAQKKKLS VNYEQCEPNS ELPQRFICKC KIGQTMYGTG SGVTKQEAKQ
160 170 180 190 200
LAAKEAYQKL LKSPPKTAGT SSSVVTSTFS GFSSSSSMTS NGVSQSAPGS
210 220 230 240 250
FSSENVFTNG LGENKRKSGV KVSPDDVQRN KYTLDARFNS DFEDIEEIGL
260 270 280 290 300
GGFGQVFKAK HRIDGKRYAI KRVKYNTEKA EHEVQALAEL NHVNIVQYHS
310 320 330 340 350
CWEGVDYDPE HSMSDTSRYK TRCLFIQMEF CDKGTLEQWM RNRNQSKVDK
360 370 380 390 400
ALILDLYEQI VTGVEYIHSK GLIHRDLKPG NIFLVDERHI KIGDFGLATA
410 420 430 440 450
LENDGKSRTR RTGTLQYMSP EQLFLKHYGK EVDIFALGLI LAELLHTCFT
460 470 480 490 500
ESEKIKFFES LRKGDFSNDI FDNKEKSLLK KLLSEKPKDR PETSEILKTL
510
AEWRNISEKK KRNTC
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 52 | P → G in AAA40150 (PubMed:1714905).Curated | 1 | |
Sequence conflicti | 60 | Q → T in AAA40150 (PubMed:1714905).Curated | 1 | |
Sequence conflicti | 87 | S → C in AAA40150 (PubMed:1714905).Curated | 1 | |
Sequence conflicti | 144 | T → I in AAA39885 (PubMed:1351683).Curated | 1 | |
Sequence conflicti | 281 – 282 | EH → DR in AAA39885 (PubMed:1351683).Curated | 2 | |
Sequence conflicti | 510 | K → E in AAA40150 (PubMed:1714905).Curated | 1 | |
Sequence conflicti | 512 – 515 | RNTC → KHMLGPF in AAA40150 (PubMed:1714905).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65029 mRNA Translation: AAA40150.1 M93567 mRNA Translation: AAA39885.1 U09928 , U09914, U09915, U09916, U09917, U09918, U09919, U09920, U09921, U09922, U09923, U09924, U09925, U09926, U09927 Genomic DNA Translation: AAC24729.1 AK028602 mRNA Translation: BAC26027.1 |
CCDSi | CCDS28980.1 |
PIRi | A59309 |
RefSeqi | NP_035293.1, NM_011163.4 |
Genome annotation databases
Ensembli | ENSMUST00000024884; ENSMUSP00000024884; ENSMUSG00000024079 |
GeneIDi | 19106 |
KEGGi | mmu:19106 |
UCSCi | uc008dph.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65029 mRNA Translation: AAA40150.1 M93567 mRNA Translation: AAA39885.1 U09928 , U09914, U09915, U09916, U09917, U09918, U09919, U09920, U09921, U09922, U09923, U09924, U09925, U09926, U09927 Genomic DNA Translation: AAC24729.1 AK028602 mRNA Translation: BAC26027.1 |
CCDSi | CCDS28980.1 |
PIRi | A59309 |
RefSeqi | NP_035293.1, NM_011163.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X48 | NMR | - | A | 96-170 | [»] | |
1X49 | NMR | - | A | 1-84 | [»] | |
SMRi | Q03963 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202376, 11 interactors |
DIPi | DIP-41411N |
IntActi | Q03963, 4 interactors |
STRINGi | 10090.ENSMUSP00000024884 |
Chemistry databases
ChEMBLi | CHEMBL1795121 |
PTM databases
iPTMneti | Q03963 |
PhosphoSitePlusi | Q03963 |
SwissPalmi | Q03963 |
Proteomic databases
EPDi | Q03963 |
jPOSTi | Q03963 |
MaxQBi | Q03963 |
PaxDbi | Q03963 |
PeptideAtlasi | Q03963 |
PRIDEi | Q03963 |
Protocols and materials databases
Antibodypediai | 3548, 1138 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000024884; ENSMUSP00000024884; ENSMUSG00000024079 |
GeneIDi | 19106 |
KEGGi | mmu:19106 |
UCSCi | uc008dph.2, mouse |
Organism-specific databases
CTDi | 5610 |
MGIi | MGI:1353449, Eif2ak2 |
Phylogenomic databases
eggNOGi | KOG1033, Eukaryota |
GeneTreei | ENSGT00940000160736 |
HOGENOMi | CLU_023682_1_0_1 |
InParanoidi | Q03963 |
OMAi | LIQMEFC |
OrthoDBi | 1195366at2759 |
PhylomeDBi | Q03963 |
TreeFami | TF317576 |
Enzyme and pathway databases
Reactomei | R-MMU-1169408, ISG15 antiviral mechanism |
Miscellaneous databases
BioGRID-ORCSi | 19106, 2 hits in 19 CRISPR screens |
ChiTaRSi | Eif2ak2, mouse |
EvolutionaryTracei | Q03963 |
PROi | PR:Q03963 |
RNActi | Q03963, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024079, Expressed in epithelium of small intestine and 275 other tissues |
Genevisiblei | Q03963, MM |
Family and domain databases
CDDi | cd00048, DSRM, 2 hits |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | E2AK2_MOUSE | |
Accessioni | Q03963Primary (citable) accession number: Q03963 Secondary accession number(s): Q61742, Q62026 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index