UniProtKB - Q03933 (HSF2_HUMAN)
Protein
Heat shock factor protein 2
Gene
HSF2
Organism
Homo sapiens (Human)
Status
Functioni
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 7 – 112 | By similarityAdd BLAST | 106 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: GO_Central
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- identical protein binding Source: Ensembl
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: ARUK-UCL
- RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- spermatogenesis Source: Ensembl
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Stress response, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q03933 |
SignaLinki | Q03933 |
SIGNORi | Q03933 |
Names & Taxonomyi
Protein namesi | Recommended name: Heat shock factor protein 2Short name: HSF 2 Alternative name(s): Heat shock transcription factor 2 Short name: HSTF 2 |
Gene namesi | Name:HSF2 Synonyms:HSTF2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:5225, HSF2 |
MIMi | 140581, gene |
neXtProti | NX_Q03933 |
VEuPathDBi | HostDB:ENSG00000025156.12 |
Subcellular locationi
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 109 | R → G: Fails to translocate to nucleus. 1 Publication | 1 | |
Mutagenesisi | 196 – 198 | RKR → ASS: Fails to translocate to nucleus. 1 Publication | 3 |
Organism-specific databases
DisGeNETi | 3298 |
OpenTargetsi | ENSG00000025156 |
PharmGKBi | PA29494 |
Miscellaneous databases
Pharosi | Q03933, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3988631 |
Genetic variation databases
BioMutai | HSF2 |
DMDMi | 462334 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124569 | 1 – 536 | Heat shock factor protein 2Add BLAST | 536 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 2 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 82 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 135 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 139 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 151 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 210 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 218 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 237 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q03933 |
jPOSTi | Q03933 |
MassIVEi | Q03933 |
MaxQBi | Q03933 |
PaxDbi | Q03933 |
PeptideAtlasi | Q03933 |
PRIDEi | Q03933 |
ProteomicsDBi | 58228 [Q03933-1] 58229 [Q03933-2] |
PTM databases
iPTMneti | Q03933 |
PhosphoSitePlusi | Q03933 |
Expressioni
Gene expression databases
Bgeei | ENSG00000025156, Expressed in female gonad and 236 other tissues |
ExpressionAtlasi | Q03933, baseline and differential |
Genevisiblei | Q03933, HS |
Organism-specific databases
HPAi | ENSG00000025156, Low tissue specificity |
Interactioni
Subunit structurei
DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.
Binary interactionsi
Hide detailsQ03933
Isoform 2 [Q03933-2]
GO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 109531, 48 interactors |
CORUMi | Q03933 |
DIPi | DIP-56593N |
IntActi | Q03933, 80 interactors |
STRINGi | 9606.ENSP00000357440 |
Miscellaneous databases
RNActi | Q03933, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q03933 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 119 – 192 | Hydrophobic repeat HR-A/BAdd BLAST | 74 | |
Regioni | 360 – 385 | Hydrophobic repeat HR-CAdd BLAST | 26 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 108 – 122 | Nuclear localization signalSequence analysisAdd BLAST | 15 | |
Motifi | 195 – 210 | Nuclear localization signalSequence analysisAdd BLAST | 16 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 170 – 173 | Poly-Gln | 4 |
Sequence similaritiesi
Belongs to the HSF family.Curated
Phylogenomic databases
eggNOGi | KOG0627, Eukaryota |
GeneTreei | ENSGT00940000155906 |
HOGENOMi | CLU_038829_1_0_1 |
InParanoidi | Q03933 |
OMAi | QMNPTDH |
OrthoDBi | 1154048at2759 |
PhylomeDBi | Q03933 |
TreeFami | TF330401 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR027712, HSF2 IPR000232, HSF_DNA-bd IPR027725, HSF_fam IPR010542, Vert_HSTF_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR10015, PTHR10015, 1 hit PTHR10015:SF185, PTHR10015:SF185, 1 hit |
Pfami | View protein in Pfam PF00447, HSF_DNA-bind, 1 hit PF06546, Vert_HS_TF, 1 hit |
PRINTSi | PR00056, HSFDOMAIN |
SMARTi | View protein in SMART SM00415, HSF, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00434, HSF_DOMAIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q03933-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP
60 70 80 90 100
KYFKHNNMAS FVRQLNMYGF RKVVHIDSGI VKQERDGPVE FQHPYFKQGQ
110 120 130 140 150
DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL
160 170 180 190 200
KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL
210 220 230 240 250
LLNTNGAQKK NLFQHIVKEP TDNHHHKVPH SRTEGLKPRE RISDDIIIYD
260 270 280 290 300
VTDDNADEEN IPVIPETNED VISDPSNCSQ YPDIVIVEDD NEDEYAPVIQ
310 320 330 340 350
SGEQNEPARE SLSSGSDGSS PLMSSAVQLN GSSSLTSEDP VTMMDSILND
360 370 380 390 400
NINLLGKVEL LDYLDSIDCS LEDFQAMLSG RQFSIDPDLL VDLFTSSVQM
410 420 430 440 450
NPTDYINNTK SENKGLETTK NNVVQPVSEE GRKSKSKPDK QLIQYTAFPL
460 470 480 490 500
LAFLDGNPAS SVEQASTTAS SEVLSSVDKP IEVDELLDSS LDPEPTQSKL
510 520 530
VRLEPLTEAE ASEATLFYLC ELAPAPLDSD MPLLDS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketV9GZ44 | V9GZ44_HUMAN | Heat shock factor protein 2 | HSF2 | 214 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041128 | 393 – 410 | Missing in isoform 2. 2 PublicationsAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65217 mRNA Translation: AAA36017.1 DQ492684 Genomic DNA Translation: ABF47087.1 AK294624 mRNA Translation: BAG57805.1 AK316377 mRNA Translation: BAH14748.1 AL121954 Genomic DNA No translation available. Z99129 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48176.1 BC112323 mRNA Translation: AAI12324.1 BC121050 mRNA Translation: AAI21051.1 BC121051 mRNA Translation: AAI21052.1 BC128420 mRNA Translation: AAI28421.1 |
CCDSi | CCDS47470.1 [Q03933-2] CCDS5124.1 [Q03933-1] |
PIRi | A41138 |
RefSeqi | NP_001129036.1, NM_001135564.1 [Q03933-2] NP_004497.1, NM_004506.3 [Q03933-1] |
Genome annotation databases
Ensembli | ENST00000368455; ENSP00000357440; ENSG00000025156 [Q03933-1] ENST00000452194; ENSP00000400380; ENSG00000025156 [Q03933-2] |
GeneIDi | 3298 |
KEGGi | hsa:3298 |
UCSCi | uc003pyu.3, human [Q03933-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65217 mRNA Translation: AAA36017.1 DQ492684 Genomic DNA Translation: ABF47087.1 AK294624 mRNA Translation: BAG57805.1 AK316377 mRNA Translation: BAH14748.1 AL121954 Genomic DNA No translation available. Z99129 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48176.1 BC112323 mRNA Translation: AAI12324.1 BC121050 mRNA Translation: AAI21051.1 BC121051 mRNA Translation: AAI21052.1 BC128420 mRNA Translation: AAI28421.1 |
CCDSi | CCDS47470.1 [Q03933-2] CCDS5124.1 [Q03933-1] |
PIRi | A41138 |
RefSeqi | NP_001129036.1, NM_001135564.1 [Q03933-2] NP_004497.1, NM_004506.3 [Q03933-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5D8K | X-ray | 1.73 | B | 8-115 | [»] | |
5D8L | X-ray | 2.07 | B/D/F/H | 8-115 | [»] | |
5HDK | X-ray | 1.32 | A/B/C/D | 7-112 | [»] | |
SMRi | Q03933 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109531, 48 interactors |
CORUMi | Q03933 |
DIPi | DIP-56593N |
IntActi | Q03933, 80 interactors |
STRINGi | 9606.ENSP00000357440 |
Chemistry databases
ChEMBLi | CHEMBL3988631 |
PTM databases
iPTMneti | Q03933 |
PhosphoSitePlusi | Q03933 |
Genetic variation databases
BioMutai | HSF2 |
DMDMi | 462334 |
Proteomic databases
EPDi | Q03933 |
jPOSTi | Q03933 |
MassIVEi | Q03933 |
MaxQBi | Q03933 |
PaxDbi | Q03933 |
PeptideAtlasi | Q03933 |
PRIDEi | Q03933 |
ProteomicsDBi | 58228 [Q03933-1] 58229 [Q03933-2] |
Protocols and materials databases
Antibodypediai | 4231, 665 antibodies |
DNASUi | 3298 |
Genome annotation databases
Ensembli | ENST00000368455; ENSP00000357440; ENSG00000025156 [Q03933-1] ENST00000452194; ENSP00000400380; ENSG00000025156 [Q03933-2] |
GeneIDi | 3298 |
KEGGi | hsa:3298 |
UCSCi | uc003pyu.3, human [Q03933-1] |
Organism-specific databases
CTDi | 3298 |
DisGeNETi | 3298 |
GeneCardsi | HSF2 |
HGNCi | HGNC:5225, HSF2 |
HPAi | ENSG00000025156, Low tissue specificity |
MIMi | 140581, gene |
neXtProti | NX_Q03933 |
OpenTargetsi | ENSG00000025156 |
PharmGKBi | PA29494 |
VEuPathDBi | HostDB:ENSG00000025156.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0627, Eukaryota |
GeneTreei | ENSGT00940000155906 |
HOGENOMi | CLU_038829_1_0_1 |
InParanoidi | Q03933 |
OMAi | QMNPTDH |
OrthoDBi | 1154048at2759 |
PhylomeDBi | Q03933 |
TreeFami | TF330401 |
Enzyme and pathway databases
PathwayCommonsi | Q03933 |
SignaLinki | Q03933 |
SIGNORi | Q03933 |
Miscellaneous databases
BioGRID-ORCSi | 3298, 26 hits in 897 CRISPR screens |
ChiTaRSi | HSF2, human |
GeneWikii | HSF2 |
GenomeRNAii | 3298 |
Pharosi | Q03933, Tbio |
PROi | PR:Q03933 |
RNActi | Q03933, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000025156, Expressed in female gonad and 236 other tissues |
ExpressionAtlasi | Q03933, baseline and differential |
Genevisiblei | Q03933, HS |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR027712, HSF2 IPR000232, HSF_DNA-bd IPR027725, HSF_fam IPR010542, Vert_HSTF_C IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR10015, PTHR10015, 1 hit PTHR10015:SF185, PTHR10015:SF185, 1 hit |
Pfami | View protein in Pfam PF00447, HSF_DNA-bind, 1 hit PF06546, Vert_HS_TF, 1 hit |
PRINTSi | PR00056, HSFDOMAIN |
SMARTi | View protein in SMART SM00415, HSF, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00434, HSF_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HSF2_HUMAN | |
Accessioni | Q03933Primary (citable) accession number: Q03933 Secondary accession number(s): B4DGJ4 Q9H445 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM