RUB1

Functionⁱ

Ubiquitin-like protein modifier that can be covalently attached to lysine residues of target proteins. Activated by the dimeric UBA3-ULA1 E1 enzyme and conjugated by the E2 UBC12 to substrate proteins. RUB1-conjugated (neddylated) substrate proteins include the cullins CDC53, RTT101 and CUL3, and the modification enhances the ubiquitin-ligase activity of the corresponding cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs).3 Publications

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functionⁱ

protein tag
Source: SGD
Inferred from direct assayⁱ
- 9531531
- 9545234

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein neddylation
Source: SGD
Inferred from direct assayⁱ
- 9531531
- 9545234

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Ubl conjugation pathway

Biological process

Ubl conjugation pathway

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29736-MONOMER
Reactomeⁱ	R-SCE-5689603 UCH proteinases R-SCE-8951664 Neddylation R-SCE-917937 Iron uptake and transport

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NEDD8-like protein RUB1 Alternative name(s): Related to ubiquitin protein 1 Ubiquitin-like protein RUB1
Gene namesⁱ	Name:RUB1 Ordered Locus Names:YDR139C ORF Names:YD9302.15C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IV

Organism-specific databases

EuPathDBⁱ	FungiDB:YDR139C
Saccharomyces Genome Database More...SGDⁱ	S000002546 RUB1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000035979	1 – 76	NEDD8-like protein RUB1Add BLAST		76
PropeptideⁱPRO_0000035980	77	Curated		1

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	76	Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMⁱ

Isopeptide bond

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q03919
PaxDbⁱ	Q03919
PRIDEⁱ	Q03919

Interactionⁱ

Subunit structureⁱ

Interacts with CDC53 and DCN1.2 Publications

Protein-protein interaction databases

BioGridⁱ	32193, 66 interactors
Database of interacting proteins More...DIPⁱ	DIP-1627N
IntActⁱ	Q03919, 13 interactors
Molecular INTeraction database More...MINTⁱ	Q03919
STRINGⁱ	4932.YDR139C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q03919
SMRⁱ	Q03919
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 74	Ubiquitin-likePROSITE-ProRule annotationAdd BLAST		74

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000141147
HOGENOMⁱ	HOG000233942
InParanoidⁱ	Q03919
KEGG Orthology (KO) More...KOⁱ	K12158
OMAⁱ	GKQMTDD
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C5P9Y

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01806 Nedd8, 1 hit
InterProⁱ	View protein in InterPro IPR038738 Nedd8-like IPR019956 Ubiquitin IPR029071 Ubiquitin-like_domsf IPR019954 Ubiquitin_CS IPR000626 Ubiquitin_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00240 ubiquitin, 1 hit
PRINTSⁱ	PR00348 UBIQUITIN
SMARTⁱ	View protein in SMART SM00213 UBQ, 1 hit
SUPFAMⁱ	SSF54236 SSF54236, 1 hit
PROSITEⁱ	View protein in PROSITE PS00299 UBIQUITIN_1, 1 hit PS50053 UBIQUITIN_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q03919-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIVKVKTLTG KEISVELKES DLVYHIKELL EEKEGIPPSQ QRLIFQGKQI 
        60         70 
DDKLTVTDAH LVEGMQLHLV LTLRGGN

Length:77

Mass (Da):8,693

Last modified:November 1, 1996 - v1

Checksum:ⁱF05DBF3C8E464FD1

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y16890 Genomic DNA Translation: CAA76516.1 Z48179 Genomic DNA Translation: CAA88221.1 BK006938 Genomic DNA Translation: DAA11982.1
PIRⁱ	S51867
NCBI Reference Sequences More...RefSeqⁱ	NP_010423.4, NM_001180446.3

Genome annotation databases

EnsemblFungiⁱ	YDR139C; YDR139C; YDR139C
GeneIDⁱ	851717
KEGGⁱ	sce:YDR139C

Protein	Similar proteins		Species	Score	Length
Q03919	Uncharacterized protein		YEAS1		77
K7_Rub1p		YEASK		77
Rub1p		YEAS8		77
Rub1p		YEASC		77
Rub1p		YEAS2		77
+1

Protein	Similar proteins		Species	Score	Length
Q03919	Uncharacterized protein		YEAS1		77
K7_Rub1p		YEASK		77
Rub1p		YEAS8		77
Rub1p		YEASC		77
Rub1p		YEAS2		77
+1

Protein	Similar proteins		Species	Score	Length
Q03919	Uncharacterized protein		YEAS1		77
K7_Rub1p		YEASK		77
Rub1p		YEAS8		77
Rub1p		YEASC		77
Rub1p		YEAS2		77
+64

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y16890 Genomic DNA Translation: CAA76516.1 Z48179 Genomic DNA Translation: CAA88221.1 BK006938 Genomic DNA Translation: DAA11982.1
PIRⁱ	S51867
NCBI Reference Sequences More...RefSeqⁱ	NP_010423.4, NM_001180446.3

3D structure databases

ProteinModelPortalⁱ	Q03919
SMRⁱ	Q03919
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32193, 66 interactors
Database of interacting proteins More...DIPⁱ	DIP-1627N
IntActⁱ	Q03919, 13 interactors
Molecular INTeraction database More...MINTⁱ	Q03919
STRINGⁱ	4932.YDR139C

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q03919
PaxDbⁱ	Q03919
PRIDEⁱ	Q03919

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YDR139C; YDR139C; YDR139C
GeneIDⁱ	851717
KEGGⁱ	sce:YDR139C

Organism-specific databases

EuPathDBⁱ	FungiDB:YDR139C
Saccharomyces Genome Database More...SGDⁱ	S000002546 RUB1

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000141147
HOGENOMⁱ	HOG000233942
InParanoidⁱ	Q03919
KEGG Orthology (KO) More...KOⁱ	K12158
OMAⁱ	GKQMTDD
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C5P9Y

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-29736-MONOMER
Reactomeⁱ	R-SCE-5689603 UCH proteinases R-SCE-8951664 Neddylation R-SCE-917937 Iron uptake and transport

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q03919

PROⁱ

PR:Q03919

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01806 Nedd8, 1 hit
InterProⁱ	View protein in InterPro IPR038738 Nedd8-like IPR019956 Ubiquitin IPR029071 Ubiquitin-like_domsf IPR019954 Ubiquitin_CS IPR000626 Ubiquitin_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00240 ubiquitin, 1 hit
PRINTSⁱ	PR00348 UBIQUITIN
SMARTⁱ	View protein in SMART SM00213 UBQ, 1 hit
SUPFAMⁱ	SSF54236 SSF54236, 1 hit
PROSITEⁱ	View protein in PROSITE PS00299 UBIQUITIN_1, 1 hit PS50053 UBIQUITIN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RUB1_YEAST
Accessionⁱ	Q03919Primary (citable) accession number: Q03919 Secondary accession number(s): D6VSC2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
	Last sequence update:	November 1, 1996
	Last modified:	July 18, 2018
	This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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UniProtKB - Q03919 (RUB1_YEAST)

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NEDD8-like protein RUB1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ