UniProtKB - Q03919 (RUB1_YEAST)
Protein
NEDD8-like protein RUB1
Gene
RUB1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Ubiquitin-like protein modifier that can be covalently attached to lysine residues of target proteins. Activated by the dimeric UBA3-ULA1 E1 enzyme and conjugated by the E2 UBC12 to substrate proteins. RUB1-conjugated (neddylated) substrate proteins include the cullins CDC53, RTT101 and CUL3, and the modification enhances the ubiquitin-ligase activity of the corresponding cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs).3 Publications
Miscellaneous
Present with 1310 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- protein tag Source: SGD
- ubiquitin protein ligase binding Source: GO_Central
GO - Biological processi
- modification-dependent protein catabolic process Source: GO_Central
- protein neddylation Source: SGD
- regulation of proteolysis Source: GO_Central
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-SCE-5689603, UCH proteinases R-SCE-8856825, Cargo recognition for clathrin-mediated endocytosis R-SCE-8951664, Neddylation |
Names & Taxonomyi
Protein namesi | Recommended name: NEDD8-like protein RUB1Alternative name(s): Related to ubiquitin protein 1 Ubiquitin-like protein RUB1 |
Gene namesi | Name:RUB1 Ordered Locus Names:YDR139C ORF Names:YD9302.15C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002546, RUB1 |
VEuPathDBi | FungiDB:YDR139C |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000035979 | 1 – 76 | NEDD8-like protein RUB1Add BLAST | 76 | |
PropeptideiPRO_0000035980 | 77 | Curated | 1 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 76 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation |
Keywords - PTMi
Isopeptide bondProteomic databases
MaxQBi | Q03919 |
PaxDbi | Q03919 |
PRIDEi | Q03919 |
PTM databases
iPTMneti | Q03919 |
Interactioni
Subunit structurei
Interacts with CDC53 and DCN1.
2 PublicationsGO - Molecular functioni
- ubiquitin protein ligase binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 32193, 68 interactors |
DIPi | DIP-1627N |
IntActi | Q03919, 9 interactors |
MINTi | Q03919 |
STRINGi | 4932.YDR139C |
Miscellaneous databases
RNActi | Q03919, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 74 | Ubiquitin-likePROSITE-ProRule annotationAdd BLAST | 74 |
Phylogenomic databases
eggNOGi | KOG0001, Eukaryota |
GeneTreei | ENSGT00940000155856 |
HOGENOMi | CLU_010412_6_4_1 |
InParanoidi | Q03919 |
OMAi | YGGKQMS |
Family and domain databases
CDDi | cd01806, Ubl_NEDD8, 1 hit |
InterProi | View protein in InterPro IPR038738, Nedd8-like IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom |
Pfami | View protein in Pfam PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q03919-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVKVKTLTG KEISVELKES DLVYHIKELL EEKEGIPPSQ QRLIFQGKQI
60 70
DDKLTVTDAH LVEGMQLHLV LTLRGGN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y16890 Genomic DNA Translation: CAA76516.1 Z48179 Genomic DNA Translation: CAA88221.1 BK006938 Genomic DNA Translation: DAA11982.1 |
PIRi | S51867 |
RefSeqi | NP_010423.4, NM_001180446.3 |
Genome annotation databases
EnsemblFungii | YDR139C_mRNA; YDR139C; YDR139C |
GeneIDi | 851717 |
KEGGi | sce:YDR139C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y16890 Genomic DNA Translation: CAA76516.1 Z48179 Genomic DNA Translation: CAA88221.1 BK006938 Genomic DNA Translation: DAA11982.1 |
PIRi | S51867 |
RefSeqi | NP_010423.4, NM_001180446.3 |
3D structure databases
SMRi | Q03919 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32193, 68 interactors |
DIPi | DIP-1627N |
IntActi | Q03919, 9 interactors |
MINTi | Q03919 |
STRINGi | 4932.YDR139C |
PTM databases
iPTMneti | Q03919 |
Proteomic databases
MaxQBi | Q03919 |
PaxDbi | Q03919 |
PRIDEi | Q03919 |
Genome annotation databases
EnsemblFungii | YDR139C_mRNA; YDR139C; YDR139C |
GeneIDi | 851717 |
KEGGi | sce:YDR139C |
Organism-specific databases
SGDi | S000002546, RUB1 |
VEuPathDBi | FungiDB:YDR139C |
Phylogenomic databases
eggNOGi | KOG0001, Eukaryota |
GeneTreei | ENSGT00940000155856 |
HOGENOMi | CLU_010412_6_4_1 |
InParanoidi | Q03919 |
OMAi | YGGKQMS |
Enzyme and pathway databases
Reactomei | R-SCE-5689603, UCH proteinases R-SCE-8856825, Cargo recognition for clathrin-mediated endocytosis R-SCE-8951664, Neddylation |
Miscellaneous databases
PROi | PR:Q03919 |
RNActi | Q03919, protein |
Family and domain databases
CDDi | cd01806, Ubl_NEDD8, 1 hit |
InterProi | View protein in InterPro IPR038738, Nedd8-like IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR019954, Ubiquitin_CS IPR019956, Ubiquitin_dom |
Pfami | View protein in Pfam PF00240, ubiquitin, 1 hit |
PRINTSi | PR00348, UBIQUITIN |
SMARTi | View protein in SMART SM00213, UBQ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00299, UBIQUITIN_1, 1 hit PS50053, UBIQUITIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RUB1_YEAST | |
Accessioni | Q03919Primary (citable) accession number: Q03919 Secondary accession number(s): D6VSC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names