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Protein

NEDD8-like protein RUB1

Gene

RUB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein modifier that can be covalently attached to lysine residues of target proteins. Activated by the dimeric UBA3-ULA1 E1 enzyme and conjugated by the E2 UBC12 to substrate proteins. RUB1-conjugated (neddylated) substrate proteins include the cullins CDC53, RTT101 and CUL3, and the modification enhances the ubiquitin-ligase activity of the corresponding cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs).3 Publications

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein tag Source: SGD

GO - Biological processi

  • protein neddylation Source: SGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-29736-MONOMER
ReactomeiR-SCE-5689603 UCH proteinases
R-SCE-8951664 Neddylation
R-SCE-917937 Iron uptake and transport

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-like protein RUB1
Alternative name(s):
Related to ubiquitin protein 1
Ubiquitin-like protein RUB1
Gene namesi
Name:RUB1
Ordered Locus Names:YDR139C
ORF Names:YD9302.15C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR139C
SGDiS000002546 RUB1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000359791 – 76NEDD8-like protein RUB1Add BLAST76
PropeptideiPRO_000003598077Curated1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond

Proteomic databases

MaxQBiQ03919
PaxDbiQ03919
PRIDEiQ03919

Interactioni

Subunit structurei

Interacts with CDC53 and DCN1.2 Publications

Protein-protein interaction databases

BioGridi32193, 66 interactors
DIPiDIP-1627N
IntActiQ03919, 13 interactors
MINTiQ03919
STRINGi4932.YDR139C

Structurei

3D structure databases

ProteinModelPortaliQ03919
SMRiQ03919
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 74Ubiquitin-likePROSITE-ProRule annotationAdd BLAST74

Phylogenomic databases

GeneTreeiENSGT00900000141147
HOGENOMiHOG000233942
InParanoidiQ03919
KOiK12158
OMAiGKQMTDD
OrthoDBiEOG092C5P9Y

Family and domain databases

CDDicd01806 Nedd8, 1 hit
InterProiView protein in InterPro
IPR038738 Nedd8-like
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVKVKTLTG KEISVELKES DLVYHIKELL EEKEGIPPSQ QRLIFQGKQI
60 70
DDKLTVTDAH LVEGMQLHLV LTLRGGN
Length:77
Mass (Da):8,693
Last modified:November 1, 1996 - v1
Checksum:iF05DBF3C8E464FD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y16890 Genomic DNA Translation: CAA76516.1
Z48179 Genomic DNA Translation: CAA88221.1
BK006938 Genomic DNA Translation: DAA11982.1
PIRiS51867
RefSeqiNP_010423.4, NM_001180446.3

Genome annotation databases

EnsemblFungiiYDR139C; YDR139C; YDR139C
GeneIDi851717
KEGGisce:YDR139C

Similar proteinsi

Entry informationi

Entry nameiRUB1_YEAST
AccessioniPrimary (citable) accession number: Q03919
Secondary accession number(s): D6VSC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: July 18, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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