UniProtKB - Q03818 (ATG16_YEAST)
Autophagy protein 16
ATG16
Functioni
Stabilizes the ATG5-ATG12 conjugate and mediates the formation of the 350 kDa complex, which is necessary for autophagy. The ATG5-ATG12/ATG16 complex is required for efficient promotion of ATG8-conjugation to phosphatidylethanolamine and ATG8 localization to the pre-autophagosomal structure (PAS). Recruits also ATG3 to the PAS. Involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress.
10 PublicationsMiscellaneous
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein-macromolecule adaptor activity Source: SGD
GO - Biological processi
- autophagosome assembly Source: ComplexPortal
- autophagosome organization Source: SGD
- autophagy Source: UniProtKB
- autophagy of mitochondrion Source: SGD
- C-terminal protein lipidation Source: SGD
- cytoplasm to vacuole transport by the Cvt pathway Source: SGD
- late nucleophagy Source: SGD
- macroautophagy Source: SGD
- piecemeal microautophagy of the nucleus Source: SGD
- protein lipidation Source: ComplexPortal
Keywordsi
Biological process | Autophagy, Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Autophagy protein 16Alternative name(s): Cytoplasm to vacuole targeting protein 11 SAP18 homolog |
Gene namesi | Name:ATG16 Synonyms:APG15, APG16, CVT11, SAP18 Ordered Locus Names:YMR159C ORF Names:YM8520.08C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004769, ATG16 |
VEuPathDBi | FungiDB:YMR159C |
Subcellular locationi
Other locations
- Preautophagosomal structure membrane 4 Publications; Peripheral membrane protein 4 Publications
Cytosol
- cytosol Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- Atg12-Atg5-Atg16 complex Source: SGD
- cytoplasm Source: SGD
- phagophore Source: SGD
- phagophore assembly site Source: SGD
- phagophore assembly site membrane Source: ComplexPortal
- vacuole-isolation membrane contact site Source: SGD
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 | R → A: Impairs interaction with ATG5and autophagy. 1 Publication | 1 | |
Mutagenesisi | 46 | F → A: Impairs interaction with ATG5and autophagy. 1 Publication | 1 | |
Mutagenesisi | 101 | D → A: Significantly reduces autophagic activity; when associated with A-102. 1 Publication | 1 | |
Mutagenesisi | 102 | E → A: Significantly reduces autophagic activity; when associated with A-101. 1 Publication | 1 | |
Mutagenesisi | 104 | I → A: Significantly reduces autophagic activity; when associated with A-108 and A-112. 1 Publication | 1 | |
Mutagenesisi | 108 | I → A: Significantly reduces autophagic activity; when associated with A-104 and A-112. 1 Publication | 1 | |
Mutagenesisi | 112 | V → A: Significantly reduces autophagic activity; when associated with A-104 and A-108. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218595 | 1 – 150 | Autophagy protein 16Add BLAST | 150 |
Proteomic databases
PaxDbi | Q03818 |
PRIDEi | Q03818 |
Interactioni
Subunit structurei
Homodimer. Part of the 350 kDa complex which is at least composed of ATG5, ATG12 and ATG16. Several units of each may be present in this complex.
Interacts directly with ATG12.
6 PublicationsBinary interactionsi
Q03818
With | #Exp. | IntAct |
---|---|---|
ATG12 [P38316] | 10 | EBI-27344,EBI-2692 |
itself | 3 | EBI-27344,EBI-27344 |
ATG5 [Q12380] | 4 | EBI-27344,EBI-2664 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 35336, 108 interactors |
ComplexPortali | CPX-1849, ATG12-ATG5-ATG16 complex |
DIPi | DIP-2144N |
IntActi | Q03818, 8 interactors |
MINTi | Q03818 |
STRINGi | 4932.YMR159C |
Miscellaneous databases
RNActi | Q03818, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q03818 |
SMRi | Q03818 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q03818 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 23 | DisorderedSequence analysisAdd BLAST | 23 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 58 – 130 | Sequence analysisAdd BLAST | 73 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 7 – 23 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502S6TV, Eukaryota |
HOGENOMi | CLU_158825_0_0_1 |
InParanoidi | Q03818 |
OMAi | QLRNKDY |
Family and domain databases
IDEALi | IID50085 |
InterProi | View protein in InterPro IPR013923, Autophagy-rel_prot_16_dom |
Pfami | View protein in Pfam PF08614, ATG16, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS
60 70 80 90 100
GAIGGNIVSH DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN
110 120 130 140 150
DELISGTIEN NVLQQKLSDL KKEHSQLVAR WLKKTEKETE AMNSEIDGTK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49705 Genomic DNA Translation: CAA89795.1 BK006946 Genomic DNA Translation: DAA10055.1 |
PIRi | S54517 |
RefSeqi | NP_013882.1, NM_001182663.1 |
Genome annotation databases
EnsemblFungii | YMR159C_mRNA; YMR159C; YMR159C |
GeneIDi | 855194 |
KEGGi | sce:YMR159C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49705 Genomic DNA Translation: CAA89795.1 BK006946 Genomic DNA Translation: DAA10055.1 |
PIRi | S54517 |
RefSeqi | NP_013882.1, NM_001182663.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DYM | X-ray | 2.20 | B/D/F/H | 1-46 | [»] | |
2DYO | X-ray | 1.97 | B | 1-57 | [»] | |
3A7O | X-ray | 2.50 | A/B/C/D/E/F | 50-123 | [»] | |
3A7P | X-ray | 2.80 | A/B | 1-150 | [»] | |
3W1S | X-ray | 2.60 | B | 1-46 | [»] | |
AlphaFoldDBi | Q03818 | |||||
SMRi | Q03818 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35336, 108 interactors |
ComplexPortali | CPX-1849, ATG12-ATG5-ATG16 complex |
DIPi | DIP-2144N |
IntActi | Q03818, 8 interactors |
MINTi | Q03818 |
STRINGi | 4932.YMR159C |
Proteomic databases
PaxDbi | Q03818 |
PRIDEi | Q03818 |
Genome annotation databases
EnsemblFungii | YMR159C_mRNA; YMR159C; YMR159C |
GeneIDi | 855194 |
KEGGi | sce:YMR159C |
Organism-specific databases
SGDi | S000004769, ATG16 |
VEuPathDBi | FungiDB:YMR159C |
Phylogenomic databases
eggNOGi | ENOG502S6TV, Eukaryota |
HOGENOMi | CLU_158825_0_0_1 |
InParanoidi | Q03818 |
OMAi | QLRNKDY |
Miscellaneous databases
EvolutionaryTracei | Q03818 |
PROi | PR:Q03818 |
RNActi | Q03818, protein |
Family and domain databases
IDEALi | IID50085 |
InterProi | View protein in InterPro IPR013923, Autophagy-rel_prot_16_dom |
Pfami | View protein in Pfam PF08614, ATG16, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG16_YEAST | |
Accessioni | Q03818Primary (citable) accession number: Q03818 Secondary accession number(s): D6VZY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families