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UniProtKB - Q03764 (EKI1_YEAST)
Protein
Ethanolamine kinase
Gene
EKI1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the committed step of phosphatidylethanolamine synthesis via the CDP-ethanolamine branch of the Kennedy pathway. Also exhibits choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway, but its preferred substrate is ethanolamine.
1 PublicationMiscellaneous
Present with 3420 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.7.1.821 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=0.171 mM for ethanolamine1 Publication
- KM=0.275 mM for choline1 Publication
- Vmax=346 nmol/min/mg enzyme for ethanolamine1 Publication
- Vmax=249 nmol/min/mg enzyme for choline1 Publication
: phosphatidylethanolamine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine. This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- choline kinase activity Source: SGD
- ethanolamine kinase activity Source: SGD
GO - Biological processi
- phosphatidylcholine biosynthetic process Source: SGD
- phosphatidylethanolamine biosynthetic process Source: SGD
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1483191, Synthesis of PC R-SCE-1483213, Synthesis of PE |
UniPathwayi | UPA00558;UER00741 |
Chemistry databases
SwissLipidsi | SLP:000000065 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:EKI1 Ordered Locus Names:YDR147W ORF Names:YD8358.04 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002554, EKI1 |
VEuPathDBi | FungiDB:YDR147W |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206230 | 1 – 534 | Ethanolamine kinaseAdd BLAST | 534 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q03764 |
PaxDbi | Q03764 |
PRIDEi | Q03764 |
PTM databases
iPTMneti | Q03764 |
Expressioni
Inductioni
Interactioni
Protein-protein interaction databases
BioGRIDi | 32201, 108 interactors |
MINTi | Q03764 |
STRINGi | 4932.YDR147W |
Miscellaneous databases
RNActi | Q03764, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the choline/ethanolamine kinase family.Curated
Phylogenomic databases
eggNOGi | KOG2686, Eukaryota |
GeneTreei | ENSGT00950000182939 |
HOGENOMi | CLU_012712_4_2_1 |
InParanoidi | Q03764 |
Family and domain databases
InterProi | View protein in InterPro IPR007521, Choline_kin_N IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF04428, Choline_kin_N, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
i Sequence
Sequence statusi: Complete.
Q03764-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYTNYSLTSS DAMPRTYLVG TASPEMSKKK RQSANCDKPT RRVIHIIDTN
60 70 80 90 100
EHSEVDLKNE LPITCTNEDG EMTSSSWTSQ TANDFLKLAY VNAKLDPSLP
110 120 130 140 150
SQYFKQDIIN VLQSLEIPGW SVPGSKESSL NKNLLTLTQI KGALTNVIYK
160 170 180 190 200
IHYPNLPPLL MRIFGDSIDS VIDREYELKV IARLSFYDLG PKLEGFFENG
210 220 230 240 250
RFEKYIEGSR TSTQADFIDR DTSIKIAKKL KELHCTVPLT HKEITDQPSC
260 270 280 290 300
WTTFDQWIKL IDSHKEWVSN NVNISENLRC SSWNFFLKSF KNYKRWLYND
310 320 330 340 350
SAFTSKLLRE DDKDSMINSG LKMVFCHNDL QHGNLLFKSK GKDDISVGDL
360 370 380 390 400
TIIDFEYAGP NPVVFDLSNH LNEWMQDYND VQSFKSHIDK YPKEEDILVF
410 420 430 440 450
AQSYINHMNE NHVKIASQEV RILYNLIIEW RPCTQLFWCL WALLQSGRLP
460 470 480 490 500
QRPLIEGEKL MSEKAGLGDE THLMEHKNKE NGKYDCSEDD SFNYLGFCKE
510 520 530
KMSVFWGDLI TLGVIDKDCP DIGKTDYLDT KLIF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z50046 Genomic DNA Translation: CAA90370.1 BK006938 Genomic DNA Translation: DAA11990.1 |
PIRi | S57974 |
RefSeqi | NP_010431.3, NM_001180454.3 |
Genome annotation databases
EnsemblFungii | YDR147W_mRNA; YDR147W; YDR147W |
GeneIDi | 851725 |
KEGGi | sce:YDR147W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z50046 Genomic DNA Translation: CAA90370.1 BK006938 Genomic DNA Translation: DAA11990.1 |
PIRi | S57974 |
RefSeqi | NP_010431.3, NM_001180454.3 |
3D structure databases
SMRi | Q03764 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32201, 108 interactors |
MINTi | Q03764 |
STRINGi | 4932.YDR147W |
Chemistry databases
SwissLipidsi | SLP:000000065 |
PTM databases
iPTMneti | Q03764 |
Proteomic databases
MaxQBi | Q03764 |
PaxDbi | Q03764 |
PRIDEi | Q03764 |
Genome annotation databases
EnsemblFungii | YDR147W_mRNA; YDR147W; YDR147W |
GeneIDi | 851725 |
KEGGi | sce:YDR147W |
Organism-specific databases
SGDi | S000002554, EKI1 |
VEuPathDBi | FungiDB:YDR147W |
Phylogenomic databases
eggNOGi | KOG2686, Eukaryota |
GeneTreei | ENSGT00950000182939 |
HOGENOMi | CLU_012712_4_2_1 |
InParanoidi | Q03764 |
Enzyme and pathway databases
UniPathwayi | UPA00558;UER00741 |
Reactomei | R-SCE-1483191, Synthesis of PC R-SCE-1483213, Synthesis of PE |
Miscellaneous databases
PROi | PR:Q03764 |
RNActi | Q03764, protein |
Family and domain databases
InterProi | View protein in InterPro IPR007521, Choline_kin_N IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF04428, Choline_kin_N, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EKI1_YEAST | |
Accessioni | Q03764Primary (citable) accession number: Q03764 Secondary accession number(s): D6VSD0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1996 | |
Last modified: | June 2, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families