UniProtKB - Q03750 (TAF8_YEAST)
Transcription initiation factor TFIID subunit 8
TAF8
Functioni
Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.
6 PublicationsMiscellaneous
GO - Biological processi
- positive regulation of transcription by RNA polymerase II Source: ComplexPortal
- RNA polymerase II preinitiation complex assembly Source: SGD
- transcription by RNA polymerase II Source: SGD
Keywordsi
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor TFIID subunit 8Alternative name(s): TAFII-65 TBP-associated factor 65 kDa TBP-associated factor 8 |
Gene namesi | Name:TAF8 Synonyms:TAF65 Ordered Locus Names:YML114C ORF Names:YM8339.05C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004582, TAF8 |
VEuPathDBi | FungiDB:YML114C |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: SGD
- transcription factor TFIID complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118887 | 1 – 510 | Transcription initiation factor TFIID subunit 8Add BLAST | 510 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 269 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q03750 |
PaxDbi | Q03750 |
PRIDEi | Q03750 |
PTM databases
iPTMneti | Q03750 |
Interactioni
Subunit structurei
In TFIID, TAF8 heterodimerizes with TAF10. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.
1 PublicationBinary interactionsi
Q03750
With | #Exp. | IntAct |
---|---|---|
TAF10 [Q12030] | 7 | EBI-27947,EBI-18889 |
Protein-protein interaction databases
BioGRIDi | 35090, 299 interactors |
ComplexPortali | CPX-1642, General transcription factor complex TFIID |
DIPi | DIP-1954N |
IntActi | Q03750, 21 interactors |
MINTi | Q03750 |
STRINGi | 4932.YML114C |
Miscellaneous databases
RNActi | Q03750, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 103 | Histone-foldAdd BLAST | 67 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 361 – 382 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 459 – 510 | DisorderedSequence analysisAdd BLAST | 52 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 142 – 165 | Sequence analysisAdd BLAST | 24 | |
Coiled coili | 346 – 383 | Sequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 459 – 498 | Polar residuesSequence analysisAdd BLAST | 40 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4336, Eukaryota |
HOGENOMi | CLU_529078_0_0_1 |
InParanoidi | Q03750 |
OMAi | YTRIQRR |
Family and domain databases
CDDi | cd08049, TAF8, 1 hit |
InterProi | View protein in InterPro IPR037818, TAF8 IPR019473, TFIID_su8_C |
PANTHERi | PTHR46338, PTHR46338, 1 hit |
Pfami | View protein in Pfam PF10406, TAF8_C, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTSKTSESGT GTQSTIVQLR NLPDLTEISH LEIDAPVVEI LKKTVLFQLN
60 70 80 90 100
SLNICISNFA LDELVNLVTV QMDGMFRNLH NLTLLQRRSQ ASQADLKLLL
110 120 130 140 150
REFNLDAPSL YQQFQASEFI KSKHSTEYEK LMSWSSLAAL PHNEEDEEDE
160 170 180 190 200
LNNIEEQQNE INVLLPPSNP LEKQIPSWLP NFPPDHTYKF TPEFNHPITD
210 220 230 240 250
LKTIKKEIVK ESQESEKALL NLNKSLSHIS SASNTPQPPG LDDEDAIEQQ
260 270 280 290 300
LEIWGNALEE RKPTITEKSF NENNIEQYAK YRVELARERV TKFEVNQLKR
310 320 330 340 350
TKNPFLKISE TLYLPESPHQ SHKTIQKTIE LQFRKSMTLF MHNLPKVQKL
360 370 380 390 400
KKEKIRMAKE ERAKSLKRRQ EELISQRTKR EQDEGHDLEL LLNNEHARDA
410 420 430 440 450
ADDTTTPNAL NNSTIVINTN AEDEDDDINL FGILGSSEDE NEMSSMPAEN
460 470 480 490 500
LVAESEPPTM TAQDTTNTTP VAHNTTNIDA TTSHSPHSTP NENAPTSPPA
510
DIATDHDITM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49210 Genomic DNA Translation: CAA89104.1 BK006946 Genomic DNA Translation: DAA09784.1 |
PIRi | S53958 |
RefSeqi | NP_013593.1, NM_001182476.1 |
Genome annotation databases
EnsemblFungii | YML114C_mRNA; YML114C; YML114C |
GeneIDi | 854926 |
KEGGi | sce:YML114C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49210 Genomic DNA Translation: CAA89104.1 BK006946 Genomic DNA Translation: DAA09784.1 |
PIRi | S53958 |
RefSeqi | NP_013593.1, NM_001182476.1 |
3D structure databases
AlphaFoldDBi | Q03750 |
SMRi | Q03750 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35090, 299 interactors |
ComplexPortali | CPX-1642, General transcription factor complex TFIID |
DIPi | DIP-1954N |
IntActi | Q03750, 21 interactors |
MINTi | Q03750 |
STRINGi | 4932.YML114C |
PTM databases
iPTMneti | Q03750 |
Proteomic databases
MaxQBi | Q03750 |
PaxDbi | Q03750 |
PRIDEi | Q03750 |
Genome annotation databases
EnsemblFungii | YML114C_mRNA; YML114C; YML114C |
GeneIDi | 854926 |
KEGGi | sce:YML114C |
Organism-specific databases
SGDi | S000004582, TAF8 |
VEuPathDBi | FungiDB:YML114C |
Phylogenomic databases
eggNOGi | KOG4336, Eukaryota |
HOGENOMi | CLU_529078_0_0_1 |
InParanoidi | Q03750 |
OMAi | YTRIQRR |
Miscellaneous databases
PROi | PR:Q03750 |
RNActi | Q03750, protein |
Family and domain databases
CDDi | cd08049, TAF8, 1 hit |
InterProi | View protein in InterPro IPR037818, TAF8 IPR019473, TFIID_su8_C |
PANTHERi | PTHR46338, PTHR46338, 1 hit |
Pfami | View protein in Pfam PF10406, TAF8_C, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TAF8_YEAST | |
Accessioni | Q03750Primary (citable) accession number: Q03750 Secondary accession number(s): D6W0H0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - SIMILARITY comments
Index of protein domains and families