The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - Q03656 (SKY1_YEAST)
Protein
Serine/threonine-protein kinase SKY1
Gene
SKY1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Constitutively active kinase, specifically and sequentially phosphorylates serine/arginine (SR)-type shuttling mRNA binding proteins in their RS dipeptide repeats.
1 PublicationMiscellaneous
Present with 2420 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 187 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 294 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 164 – 172 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: SGD
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular cation homeostasis Source: SGD
- cellular ion homeostasis Source: SGD
- intracellular signal transduction Source: GO_Central
- mRNA splice site selection Source: SGD
- peptidyl-serine phosphorylation Source: SGD
- positive regulation of protein import into nucleus Source: SGD
- protein phosphorylation Source: SGD
- regulation of cell size Source: SGD
- regulation of mRNA processing Source: GO_Central
- response to xenobiotic stimulus Source: SGD
- spliceosomal complex assembly Source: GO_Central
- stress granule disassembly Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:SKY1 Ordered Locus Names:YMR216C ORF Names:YM8261.10C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004829, SKY1 |
VEuPathDBi | FungiDB:YMR216C |
Subcellular locationi
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086658 | 1 – 742 | Serine/threonine-protein kinase SKY1Add BLAST | 742 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 383 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 386 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 388 | PhosphoserineCombined sources | 1 | |
Modified residuei | 393 | PhosphoserineCombined sources | 1 | |
Modified residuei | 410 | PhosphoserineCombined sources | 1 | |
Modified residuei | 427 | PhosphoserineCombined sources | 1 | |
Modified residuei | 432 | PhosphoserineCombined sources | 1 | |
Modified residuei | 445 | PhosphoserineCombined sources | 1 | |
Modified residuei | 449 | PhosphoserineCombined sources | 1 | |
Modified residuei | 453 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q03656 |
PaxDbi | Q03656 |
PRIDEi | Q03656 |
PTM databases
iPTMneti | Q03656 |
Interactioni
Binary interactionsi
Q03656
With | #Exp. | IntAct |
---|---|---|
TOR1 [P35169] | 2 | EBI-9800,EBI-19374 |
Protein-protein interaction databases
BioGRIDi | 35394, 412 interactors |
DIPi | DIP-2884N |
IntActi | Q03656, 16 interactors |
MINTi | Q03656 |
STRINGi | 4932.YMR216C |
Miscellaneous databases
RNActi | Q03656, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q03656 |
SMRi | Q03656 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q03656 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 158 – 706 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 549 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 146 | DisorderedSequence analysisAdd BLAST | 134 | |
Regioni | 350 – 369 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 459 – 491 | DisorderedSequence analysisAdd BLAST | 33 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 41 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 59 – 83 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 84 – 101 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 102 – 122 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 124 – 140 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 350 – 365 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1290, Eukaryota |
GeneTreei | ENSGT00940000170102 |
HOGENOMi | CLU_000288_81_8_1 |
InParanoidi | Q03656 |
OMAi | NTTGKHV |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
Q03656-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSSINYPGF VTKSAHLADT STDASISCEE ATSSQEAKKN FFQRDYNMMK
60 70 80 90 100
KAPAPTKSKL SLALQTSKSS SSANGTVQED TSSKTEDFST KSIKKKPDSG
110 120 130 140 150
VESHVSIQSD SGPQSDSDLD SDSSISSCDE RNEESLKDYR PGGYHPAFKG
160 170 180 190 200
EPYKDARYIL VRKLGWGHFS TVWLAKDMVN NTHVAMKIVR GDKVYTEAAE
210 220 230 240 250
DEIKLLQRVN DADNTKEDSM GANHILKLLD HFNHKGPNGV HVVMVFEVLG
260 270 280 290 300
ENLLALIKKY EHRGIPLIYV KQISKQLLLG LDYMHRRCGI IHTDIKPENV
310 320 330 340 350
LMEIGDVEGI VQMVEALDKQ KREAKRLQRH VSRSSDITAN DSSDEKWAEC
360 370 380 390 400
QTSMPCGSSS NSKSRSIEKD LSKRCFRRPR RHTIITGSQP LPSPISSSNF
410 420 430 440 450
FEMRAHFCGS SHNSFSSVSG NRNIPSSINN NSINNGIGIK NSNNSFLNSV
460 470 480 490 500
PHSVTRMFIN EDSNDNNNND NSKNKNNNNN NSNNNNNEDI MNTPLHEEQL
510 520 530 540 550
ADSLSTFDIS NISQSSDTNG PYISNTMDSN SNVSTDINSP ENLIQIKIAD
560 570 580 590 600
LGNACWYDEH YTNSIQTREY RSPEVLLGAP WGCGADIWST ACLIFELITG
610 620 630 640 650
DFLFEPDEGH SYTKDDDHIA QIIELLGELP SYLLRNGKYT RTFFNSRGLL
660 670 680 690 700
RNISKLKFWP LEDVLTEKYK FSKDEAKEIS DFLSPMLQLD PRKRADAGGL
710 720 730 740
VNHPWLKDTL GMEEIRVPDR ELYGSGSDIP GWFEEVRDHK RH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49809 Genomic DNA Translation: CAA89931.1 BK006946 Genomic DNA Translation: DAA10115.1 |
PIRi | S55098 |
RefSeqi | NP_013943.1, NM_001182723.1 |
Genome annotation databases
EnsemblFungii | YMR216C_mRNA; YMR216C; YMR216C |
GeneIDi | 855256 |
KEGGi | sce:YMR216C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z49809 Genomic DNA Translation: CAA89931.1 BK006946 Genomic DNA Translation: DAA10115.1 |
PIRi | S55098 |
RefSeqi | NP_013943.1, NM_001182723.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HOW | X-ray | 2.10 | A | 138-304 | [»] | |
A | 539-742 | [»] | ||||
1Q8Y | X-ray | 2.05 | A/B | 138-304 | [»] | |
A/B | 539-742 | [»] | ||||
1Q8Z | X-ray | 2.35 | A/B | 138-304 | [»] | |
A/B | 539-742 | [»] | ||||
1Q97 | X-ray | 2.30 | A/B | 138-304 | [»] | |
A/B | 539-742 | [»] | ||||
1Q99 | X-ray | 2.11 | A/B | 138-304 | [»] | |
A/B | 539-742 | [»] | ||||
2JD5 | X-ray | 2.50 | A/B | 138-742 | [»] | |
AlphaFoldDBi | Q03656 | |||||
SMRi | Q03656 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35394, 412 interactors |
DIPi | DIP-2884N |
IntActi | Q03656, 16 interactors |
MINTi | Q03656 |
STRINGi | 4932.YMR216C |
PTM databases
iPTMneti | Q03656 |
Proteomic databases
MaxQBi | Q03656 |
PaxDbi | Q03656 |
PRIDEi | Q03656 |
Genome annotation databases
EnsemblFungii | YMR216C_mRNA; YMR216C; YMR216C |
GeneIDi | 855256 |
KEGGi | sce:YMR216C |
Organism-specific databases
SGDi | S000004829, SKY1 |
VEuPathDBi | FungiDB:YMR216C |
Phylogenomic databases
eggNOGi | KOG1290, Eukaryota |
GeneTreei | ENSGT00940000170102 |
HOGENOMi | CLU_000288_81_8_1 |
InParanoidi | Q03656 |
OMAi | NTTGKHV |
Miscellaneous databases
EvolutionaryTracei | Q03656 |
PROi | PR:Q03656 |
RNActi | Q03656, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SKY1_YEAST | |
Accessioni | Q03656Primary (citable) accession number: Q03656 Secondary accession number(s): D6W041 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 186 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families