UniProtKB - Q03558 (OYE2_YEAST)
Protein
NADPH dehydrogenase 2
Gene
OYE2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Flavin-dependent enoate reductase that catalyzes the chemo- and stereoslective hydrogenation of electron-poor alkenes. The enzyme is reduced by NADPH, and oxygen, quinones, and alpha,beta-unsaturated aldehydes and ketones can act as electron acceptors to complete catalytic turnover. The physiological oxidant remains elusive (By similarity). Has an antioxidant activity, reducing reactive oxygen species (ROS) levels when overexpressed. Formation of OYE2-OYE3 heterodimers contribute to the induction of programmed cell death upon oxidative stress (PubMed:17897954).By similarity1 Publication
Miscellaneous
Present with 15100 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:1.6.99.11 Publication
Cofactori
FMN1 Publication
Kineticsi
- KM=1 µM for cyclohexenone1 Publication
- KM=1.65 mM for oxygen1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | FMNBy similarity | 1 | |
Binding sitei | 115 | FMNBy similarity | 1 | |
Binding sitei | 192 | SubstrateBy similarity | 1 | |
Binding sitei | 195 | SubstrateBy similarity | 1 | |
Active sitei | 197 | Proton donorBy similarity | 1 | |
Binding sitei | 244 | FMNBy similarity | 1 | |
Binding sitei | 349 | FMNBy similarity | 1 | |
Binding sitei | 376 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- FMN binding Source: InterPro
- NADPH dehydrogenase activity Source: SGD
- oxidoreductase activity Source: GO_Central
- pentaerythritol trinitrate reductase activity Source: UniProtKB-EC
GO - Biological processi
- apoptotic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN, NADP |
Enzyme and pathway databases
BioCyci | MetaCyc:YHR179W-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH dehydrogenase 21 Publication (EC:1.6.99.11 Publication)Alternative name(s): Old yellow enzyme 21 Publication |
Gene namesi | Name:OYE21 Publication Ordered Locus Names:YHR179W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YHR179W |
SGDi | S000001222, OYE2 |
Subcellular locationi
Mitochondrion
- Mitochondrion 2 Publications
Nucleus
- Nucleus 1 Publication
Other locations
- Cytoplasm 1 Publication
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000194474 | 2 – 400 | NADPH dehydrogenase 2Add BLAST | 399 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 353 | PhosphoserineCombined sources | 1 | |
Modified residuei | 379 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q03558 |
PaxDbi | Q03558 |
PRIDEi | Q03558 |
TopDownProteomicsi | Q03558 |
PTM databases
iPTMneti | Q03558 |
Interactioni
Subunit structurei
Homodimer or heterodimer with OYE3.
1 PublicationBinary interactionsi
Q03558
With | #Exp. | IntAct |
---|---|---|
OYE3 [P41816] | 2 | EBI-12729,EBI-12734 |
Protein-protein interaction databases
BioGRIDi | 36612, 113 interactors |
DIPi | DIP-213N |
IntActi | Q03558, 29 interactors |
MINTi | Q03558 |
STRINGi | 4932.YHR179W |
Miscellaneous databases
RNActi | Q03558, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.Curated
Phylogenomic databases
eggNOGi | KOG0134, Eukaryota |
GeneTreei | ENSGT00940000176560 |
HOGENOMi | CLU_012153_0_0_1 |
InParanoidi | Q03558 |
OMAi | MQIMHGG |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001155, OxRdtase_FMN_N |
Pfami | View protein in Pfam PF00724, Oxidored_FMN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q03558-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPFVKDFKPQ ALGDTNLFKP IKIGNNELLH RAVIPPLTRM RAQHPGNIPN
60 70 80 90 100
RDWAVEYYAQ RAQRPGTLII TEGTFPSPQS GGYDNAPGIW SEEQIKEWTK
110 120 130 140 150
IFKAIHENKS FAWVQLWVLG WAAFPDTLAR DGLRYDSASD NVYMNAEQEE
160 170 180 190 200
KAKKANNPQH SITKDEIKQY VKEYVQAAKN SIAAGADGVE IHSANGYLLN
210 220 230 240 250
QFLDPHSNNR TDEYGGSIEN RARFTLEVVD AVVDAIGPEK VGLRLSPYGV
260 270 280 290 300
FNSMSGGAET GIVAQYAYVL GELERRAKAG KRLAFVHLVE PRVTNPFLTE
310 320 330 340 350
GEGEYNGGSN KFAYSIWKGP IIRAGNFALH PEVVREEVKD PRTLIGYGRF
360 370 380 390 400
FISNPDLVDR LEKGLPLNKY DRDTFYKMSA EGYIDYPTYE EALKLGWDKN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 90 | W → R in AAS56612 (PubMed:17322287).Curated | 1 |
Mass spectrometryi
Molecular mass is 44886 Da. Determined by ESI. 1 Publication
Molecular mass is 44865 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06124 Genomic DNA Translation: AAA83386.1 U00027 Genomic DNA Translation: AAB68024.1 AY558286 Genomic DNA Translation: AAS56612.1 BK006934 Genomic DNA Translation: DAA06872.1 |
PIRi | A46009 |
RefSeqi | NP_012049.1, NM_001179310.1 |
Genome annotation databases
EnsemblFungii | YHR179W_mRNA; YHR179W; YHR179W |
GeneIDi | 856584 |
KEGGi | sce:YHR179W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L06124 Genomic DNA Translation: AAA83386.1 U00027 Genomic DNA Translation: AAB68024.1 AY558286 Genomic DNA Translation: AAS56612.1 BK006934 Genomic DNA Translation: DAA06872.1 |
PIRi | A46009 |
RefSeqi | NP_012049.1, NM_001179310.1 |
3D structure databases
SMRi | Q03558 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36612, 113 interactors |
DIPi | DIP-213N |
IntActi | Q03558, 29 interactors |
MINTi | Q03558 |
STRINGi | 4932.YHR179W |
PTM databases
iPTMneti | Q03558 |
Proteomic databases
MaxQBi | Q03558 |
PaxDbi | Q03558 |
PRIDEi | Q03558 |
TopDownProteomicsi | Q03558 |
Genome annotation databases
EnsemblFungii | YHR179W_mRNA; YHR179W; YHR179W |
GeneIDi | 856584 |
KEGGi | sce:YHR179W |
Organism-specific databases
EuPathDBi | FungiDB:YHR179W |
SGDi | S000001222, OYE2 |
Phylogenomic databases
eggNOGi | KOG0134, Eukaryota |
GeneTreei | ENSGT00940000176560 |
HOGENOMi | CLU_012153_0_0_1 |
InParanoidi | Q03558 |
OMAi | MQIMHGG |
Enzyme and pathway databases
BioCyci | MetaCyc:YHR179W-MONOMER |
Miscellaneous databases
PROi | PR:Q03558 |
RNActi | Q03558, protein |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR001155, OxRdtase_FMN_N |
Pfami | View protein in Pfam PF00724, Oxidored_FMN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OYE2_YEAST | |
Accessioni | Q03558Primary (citable) accession number: Q03558 Secondary accession number(s): D3DLC8, E9P8V8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names