UniProtKB - Q03524 (SP5D_BACSU)
Protein
Stage V sporulation protein D
Gene
spoVD
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Penicillin-binding protein with an unknown catalytic activity. May have a specialized role in the morphogenesis of spore cortex, which is a modified form of peptidoglycan. Spore cortex formation is determined primarily by the mother cell.1 Publication
Catalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.Curated EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 294 | Acyl-ester intermediateBy similarity | 1 |
GO - Molecular functioni
- penicillin binding Source: GO_Central
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC
GO - Biological processi
- cell wall organization Source: GO_Central
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- regulation of cell shape Source: UniProtKB-KW
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Sporulation |
Enzyme and pathway databases
BioCyci | BSUB:BSU15170-MONOMER |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: Stage V sporulation protein D (EC:3.4.16.4Curated)Alternative name(s): Sporulation-specific penicillin-binding protein |
Gene namesi | Name:spoVD Ordered Locus Names:BSU15170 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein Curated
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Asporogeneous; spore development starts but does not develop the spore cortex.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195466 | 1 – 646 | Stage V sporulation protein DAdd BLAST | 646 |
Proteomic databases
PaxDbi | Q03524 |
PRIDEi | Q03524 |
Expressioni
Developmental stagei
Expressed during sporulation; strong expression 60-150 minutes after sporulation onset, falls to background expression by 240 minutes. Found only in mother cells.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 580 – 638 | PASTAPROSITE-ProRule annotationAdd BLAST | 59 |
Sequence similaritiesi
Belongs to the transpeptidase family.Curated
Phylogenomic databases
eggNOGi | COG0768, Bacteria |
InParanoidi | Q03524 |
OMAi | DPQIVVY |
PhylomeDBi | Q03524 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005543, PASTA_dom IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept IPR011927, SpoVD_pbp |
Pfami | View protein in Pfam PF03793, PASTA, 1 hit PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SMARTi | View protein in SMART SM00740, PASTA, 1 hit |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR02214, spoVD_pbp, 1 hit |
PROSITEi | View protein in PROSITE PS51178, PASTA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q03524-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVSNVTVRK RLLFVLLFGV IVFLIIDTRL GYVQFVMGEK LTSLAKDSWS
60 70 80 90 100
RNLPFEPERG EILDRNGVKL ATNKSAPTVF VVPRQVQNPM KTSKQLAAVL
110 120 130 140 150
NMSEEKVYKH VTKKASIEKI TPEGRKISNE KAKEIKALDL KGVYVAEDSI
160 170 180 190 200
RHYPFGSFLS HVLGFAGIDN QGLLGLEAYY DDDLKGEKGS VKFYTDAKGK
210 220 230 240 250
KMPDEADDYT PPKDGLDMKL TVDSKVQTIM ERELDNAEAK YHPDGMIAVA
260 270 280 290 300
MNPKNGEILG MSSRPDFDPA DYQSVDPSVY NRNLPVWSTY EPGSTFKIIT
310 320 330 340 350
LAAALEEQKV NLKRDQFYDK GHAEVDGARL RCWKRGGHGL QTYLEVVQNS
360 370 380 390 400
CNPGFVELGE RLGKEKLFKY IKDFGFGQKT GIDLQGEGTG ILFPLERVGP
410 420 430 440 450
VEQATTAFGQ GVSVTPIQQV AAVSAAVNGG TLYTPYIAKE WIDPVTKKTV
460 470 480 490 500
KKQSPIAKKQ VISEETSKQI RYALESVVAE GTGRNAFVEG YRVGGKTGTA
510 520 530 540 550
QKVKDGKYLE NNHIVSFIGF APADDPSLVV YVAVDNPKGT IQFGGTVAAP
560 570 580 590 600
IVGNIMRDSL PELGVKPRKN QIEKKYQWND TKTIEVPNVV GMSVSDLESL
610 620 630 640
LVNLNVDASG KGSKIVKQSP AAGTKVKEGS KIRVYLTEED EKEAAD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 540 – 549 | TIQFGGTVAA → AFSLAERWQ in CAA81085 (PubMed:8289242).Curated | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25865 Genomic DNA Translation: CAA81085.1 AL009126 Genomic DNA Translation: CAB13390.2 L09703 Unassigned DNA Translation: AAC36838.1 Z15056 Genomic DNA Translation: CAA78766.1 Z68230 Genomic DNA Translation: CAA92528.1 |
PIRi | S49570 |
RefSeqi | NP_389400.2, NC_000964.3 WP_003245048.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13390; CAB13390; BSU_15170 |
GeneIDi | 936661 |
KEGGi | bsu:BSU15170 |
PATRICi | fig|224308.179.peg.1653 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z25865 Genomic DNA Translation: CAA81085.1 AL009126 Genomic DNA Translation: CAB13390.2 L09703 Unassigned DNA Translation: AAC36838.1 Z15056 Genomic DNA Translation: CAA78766.1 Z68230 Genomic DNA Translation: CAA92528.1 |
PIRi | S49570 |
RefSeqi | NP_389400.2, NC_000964.3 WP_003245048.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | Q03524 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU15170 |
Proteomic databases
PaxDbi | Q03524 |
PRIDEi | Q03524 |
Genome annotation databases
EnsemblBacteriai | CAB13390; CAB13390; BSU_15170 |
GeneIDi | 936661 |
KEGGi | bsu:BSU15170 |
PATRICi | fig|224308.179.peg.1653 |
Phylogenomic databases
eggNOGi | COG0768, Bacteria |
InParanoidi | Q03524 |
OMAi | DPQIVVY |
PhylomeDBi | Q03524 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | BSUB:BSU15170-MONOMER |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005543, PASTA_dom IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept IPR011927, SpoVD_pbp |
Pfami | View protein in Pfam PF03793, PASTA, 1 hit PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SMARTi | View protein in SMART SM00740, PASTA, 1 hit |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
TIGRFAMsi | TIGR02214, spoVD_pbp, 1 hit |
PROSITEi | View protein in PROSITE PS51178, PASTA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SP5D_BACSU | |
Accessioni | Q03524Primary (citable) accession number: Q03524 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | June 16, 2009 | |
Last modified: | April 7, 2021 | |
This is version 139 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families