UniProtKB - Q03522 (MURD_BACSU)
Protein
UDP-N-acetylmuramoylalanine--D-glutamate ligase
Gene
murD
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).By similarity
Catalytic activityi
- ATP + D-glutamate + UDP-N-acetyl-α-D-muramoyl-L-alanine = ADP + H+ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamateEC:6.3.2.9
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 120 – 126 | ATPSequence analysis | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU15200-MONOMER |
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC:6.3.2.9)Alternative name(s): D-glutamic acid-adding enzyme UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase |
Gene namesi | Name:murD Ordered Locus Names:BSU15200 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000108966 | 1 – 451 | UDP-N-acetylmuramoylalanine--D-glutamate ligaseAdd BLAST | 451 |
Proteomic databases
PaxDbi | Q03522 |
PRIDEi | Q03522 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MurCDEF family.Curated
Phylogenomic databases
eggNOGi | COG0771, Bacteria |
InParanoidi | Q03522 |
OMAi | ACASYDQ |
PhylomeDBi | Q03522 |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_00639, MurD, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR005762, MurD |
Pfami | View protein in Pfam PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit |
TIGRFAMsi | TIGR01087, murD, 1 hit |
i Sequence
Sequence statusi: Complete.
Q03522-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MENDQFLQKQ HFLILGLAKS GYAAASILHE KGIYVAVNDQ KPFEENEPAQ
60 70 80 90 100
KLSEKGIEVV CGEHPVSLFD QHQITILIKN PGIPYENIMV QEAEKRGIPV
110 120 130 140 150
WTEIELAYYL TSAKFIGITG SNGKTTTTTL IYEMLKADSQ KALIAGNIGT
160 170 180 190 200
VASEVAYHAD GDEWIVTELS SFQLMGTHAF RPEISLILNV FDAHLDYHHT
210 220 230 240 250
RENYEKAKQK VYLHQTASDK AIVNQDDETV VRLAEAGKAE IVPFSVSKTL
260 270 280 290 300
EQGAYVKDSM IMFNGEAILP LEEVVLPGAH NLENILAAIA VVKTAGASNE
310 320 330 340 350
AVKKVLTSFT GVKHRLQYVT TVNGRKFYND SKATNILATS KALSAFDKPV
360 370 380 390 400
ILLAGGLDRG NGFDDLKPYM KHVKAVLTFG QTAPKLEKLG NELGIQHVKR
410 420 430 440 450
VDNVEQAVSA AFALSNEGDV ILLSPACASW DQFKTFEERG DMFIDAVHML
K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 342 | A → R in CAA45555 (PubMed:1391053).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z15056 Genomic DNA Translation: CAA78769.1 AL009126 Genomic DNA Translation: CAB13393.1 X64258 Genomic DNA Translation: CAA45555.1 |
PIRi | D47691 |
RefSeqi | NP_389403.1, NC_000964.3 WP_003232189.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13393; CAB13393; BSU_15200 |
GeneIDi | 935950 |
KEGGi | bsu:BSU15200 |
PATRICi | fig|224308.179.peg.1658 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z15056 Genomic DNA Translation: CAA78769.1 AL009126 Genomic DNA Translation: CAB13393.1 X64258 Genomic DNA Translation: CAA45555.1 |
PIRi | D47691 |
RefSeqi | NP_389403.1, NC_000964.3 WP_003232189.1, NZ_JNCM01000035.1 |
3D structure databases
SMRi | Q03522 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU15200 |
Proteomic databases
PaxDbi | Q03522 |
PRIDEi | Q03522 |
Protocols and materials databases
DNASUi | 935950 |
Genome annotation databases
EnsemblBacteriai | CAB13393; CAB13393; BSU_15200 |
GeneIDi | 935950 |
KEGGi | bsu:BSU15200 |
PATRICi | fig|224308.179.peg.1658 |
Phylogenomic databases
eggNOGi | COG0771, Bacteria |
InParanoidi | Q03522 |
OMAi | ACASYDQ |
PhylomeDBi | Q03522 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | BSUB:BSU15200-MONOMER |
Family and domain databases
Gene3Di | 3.40.1190.10, 1 hit 3.90.190.20, 1 hit |
HAMAPi | MF_00639, MurD, 1 hit |
InterProi | View protein in InterPro IPR036565, Mur-like_cat_sf IPR004101, Mur_ligase_C IPR036615, Mur_ligase_C_dom_sf IPR013221, Mur_ligase_cen IPR005762, MurD |
Pfami | View protein in Pfam PF02875, Mur_ligase_C, 1 hit PF08245, Mur_ligase_M, 1 hit |
SUPFAMi | SSF53244, SSF53244, 1 hit SSF53623, SSF53623, 1 hit |
TIGRFAMsi | TIGR01087, murD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURD_BACSU | |
Accessioni | Q03522Primary (citable) accession number: Q03522 Secondary accession number(s): Q59246 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | April 7, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families