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Protein

Antigen peptide transporter 2

Gene

TAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi503 – 510ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • MHC class Ib protein binding Source: UniProtKB
  • peptide antigen-transporting ATPase activity Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB
  • transporter activity Source: MGI

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Host-virus interaction, Immunity, Peptide transport, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.43 2681
ReactomeiR-HSA-1236974 ER-Phagosome pathway
R-HSA-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Protein family/group databases

TCDBi3.A.1.209.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen peptide transporter 2
Short name:
APT2
Alternative name(s):
ATP-binding cassette sub-family B member 3
Peptide supply factor 2
Peptide transporter PSF2
Short name:
PSF-2
Peptide transporter TAP2
Peptide transporter involved in antigen processing 2
Really interesting new gene 11 protein
Gene namesi
Name:TAP2
Synonyms:ABCB3, PSF2, RING11, Y1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204267.13
HGNCiHGNC:44 TAP2
MIMi170261 gene
neXtProtiNX_Q03519

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalSequence analysis6
Transmembranei7 – 27Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini28 – 56CytoplasmicSequence analysisAdd BLAST29
Transmembranei57 – 77Helical; Name=2PROSITE-ProRule annotationAdd BLAST21
Topological domaini78 – 98LumenalSequence analysisAdd BLAST21
Transmembranei99 – 119Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini120 – 148CytoplasmicSequence analysisAdd BLAST29
Transmembranei149 – 169Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini170 – 187LumenalSequence analysisAdd BLAST18
Transmembranei188 – 208Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini209 – 266CytoplasmicSequence analysisAdd BLAST58
Transmembranei267 – 287Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini288 – 293LumenalSequence analysis6
Transmembranei294 – 314Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini315 – 374CytoplasmicSequence analysisAdd BLAST60
Transmembranei375 – 395Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini396 – 408LumenalSequence analysisAdd BLAST13
Transmembranei409 – 429Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini430 – 686CytoplasmicSequence analysisAdd BLAST257

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Involvement in diseasei

Bare lymphocyte syndrome 1 (BLS1)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA HLA class I deficiency. Contrary to bare lymphocyte syndromes type 2 and type 3, which are characterized by early-onset severe combined immunodeficiency, class I antigen deficiencies are not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
See also OMIM:604571

Organism-specific databases

DisGeNETi6891
MalaCardsiTAP2
MIMi604571 phenotype
OpenTargetsiENSG00000204267
Orphaneti34592 Immunodeficiency by defective expression of HLA class 1
PharmGKBiPA35022

Polymorphism and mutation databases

BioMutaiTAP2
DMDMi549044

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933291 – 686Antigen peptide transporter 2Add BLAST686

Proteomic databases

EPDiQ03519
PaxDbiQ03519
PeptideAtlasiQ03519
PRIDEiQ03519
ProteomicsDBi58215
58216 [Q03519-2]
TopDownProteomicsiQ03519-2 [Q03519-2]

PTM databases

iPTMnetiQ03519
PhosphoSitePlusiQ03519

Expressioni

Inductioni

By IFNG/IFN-gamma.

Gene expression databases

BgeeiENSG00000204267
CleanExiHS_TAP2
ExpressionAtlasiQ03519 baseline and differential
GenevisibleiQ03519 HS

Organism-specific databases

HPAiHPA001312

Interactioni

Subunit structurei

Heterodimer of TAP1 and TAP2. Interacts with Epstein-Barr virus BLNF2a. Interacts with herpes simplex virus ICP47 (PubMed:7760936).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • MHC class Ib protein binding Source: UniProtKB
  • TAP1 binding Source: UniProtKB
  • tapasin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112754, 35 interactors
DIPiDIP-322N
IntActiQ03519, 23 interactors
MINTiQ03519
STRINGi9606.ENSP00000364034

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5U1Delectron microscopy3.97B1-686[»]
ProteinModelPortaliQ03519
SMRiQ03519
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini152 – 435ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini468 – 686ABC transporterPROSITE-ProRule annotationAdd BLAST219

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni301 – 389Involved in peptide-binding siteAdd BLAST89
Regioni414 – 433Involved in peptide-binding siteAdd BLAST20

Domaini

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058 Eukaryota
COG1132 LUCA
GeneTreeiENSGT00550000074497
HOVERGENiHBG008358
InParanoidiQ03519
KOiK05654
PhylomeDBiQ03519
TreeFamiTF105197

Family and domain databases

Gene3Di1.20.1560.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR013305 ABC_Tap-like
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR005293 Tap2/ABCB3
PfamiView protein in Pfam
PF00664 ABC_membrane, 1 hit
PF00005 ABC_tran, 1 hit
PRINTSiPR01897 TAP2PROTEIN
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF90123 SSF90123, 1 hit
TIGRFAMsiTIGR00958 3a01208, 1 hit
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 1 hit
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03519-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLPDLRPWT SLLLVDAALL WLLQGPLGTL LPQGLPGLWL EGTLRLGGLW
60 70 80 90 100
GLLKLRGLLG FVGTLLLPLC LATPLTVSLR ALVAGASRAP PARVASAPWS
110 120 130 140 150
WLLVGYGAAG LSWSLWAVLS PPGAQEKEQD QVNNKVLMWR LLKLSRPDLP
160 170 180 190 200
LLVAAFFFLV LAVLGETLIP HYSGRVIDIL GGDFDPHAFA SAIFFMCLFS
210 220 230 240 250
FGSSLSAGCR GGCFTYTMSR INLRIREQLF SSLLRQDLGF FQETKTGELN
260 270 280 290 300
SRLSSDTTLM SNWLPLNANV LLRSLVKVVG LYGFMLSISP RLTLLSLLHM
310 320 330 340 350
PFTIAAEKVY NTRHQEVLRE IQDAVARAGQ VVREAVGGLQ TVRSFGAEEH
360 370 380 390 400
EVCRYKEALE QCRQLYWRRD LERALYLLVR RVLHLGVQML MLSCGLQQMQ
410 420 430 440 450
DGELTQGSLL SFMIYQESVG SYVQTLVYIY GDMLSNVGAA EKVFSYMDRQ
460 470 480 490 500
PNLPSPGTLA PTTLQGVVKF QDVSFAYPNR PDRPVLKGLT FTLRPGEVTA
510 520 530 540 550
LVGPNGSGKS TVAALLQNLY QPTGGQVLLD EKPISQYEHC YLHSQVVSVG
560 570 580 590 600
QEPVLFSGSV RNNIAYGLQS CEDDKVMAAA QAAHADDFIQ EMEHGIYTDV
610 620 630 640 650
GEKGSQLAAG QKQRLAIARA LVRDPRVLIL DEATSALDVQ CEQALQDWNS
660 670 680
RGDRTVLVIA HRLQTVQRAH QILVLQEGKL QKLAQL
Length:686
Mass (Da):75,664
Last modified:June 1, 1994 - v1
Checksum:iE7E4A7F6A2A3B48B
GO
Isoform 2 (identifier: Q03519-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     645-686: LQDWNSRGDRTVLVIAHRLQTVQRAHQILVLQEGKLQKLAQL → KTLWKFMIF

Show »
Length:653
Mass (Da):72,003
Checksum:iAA79C3E9C6FE31AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti345F → I in BAD97020 (Ref. 8) Curated1
Sequence conflicti520Y → N in BAD97020 (Ref. 8) Curated1
Sequence conflicti655T → A in BAD97020 (Ref. 8) Curated1

Polymorphismi

4 common alleles are officially recognized: TAP2*01:01 (TAP2A or PSF2A or RING11A), TAP2*01:02 (TAP2E), TAP2*01:03 (TAP2F), and TAP2*02:01 (TAP2B or PSF2B or RING11B). Other relatively common alleles have been identified: TAP2*01D, TAP2*01E, TAP2*01F, TAP2*01G, TAP2*01H, TAP2*02B, TAP2*02C (TAP2*02:02), TAP2*02D, TAP2*02E, TAP2*02F, TAP2*03A and TAP2*04A. The sequence shown is that of TAP2*01:01.6 Publications
The allele TAP2*Bky2 is commonly found only in the Japanese population. It may be associated with susceptibility to Sjoegren syndrome, an autoimmune disorder characterized by abnormal dryness of the conjunctiva, cornea and mouth due to exocrine glands dysfunction.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03687356R → K. Corresponds to variant dbSNP:rs17220192Ensembl.1
Natural variantiVAR_014997374A → T in allele TAP2*01F, allele TAP2*01G, allele TAP2*01H, allele TAP2*02B and allele TAP2*02D. 2 PublicationsCorresponds to variant dbSNP:rs111303994EnsemblClinVar.1
Natural variantiVAR_000094379V → I in allele TAP2*01D, allele TAP2*01E, allele TAP2*01G, allele TAP2*02C and allele TAP2*02F. 3 PublicationsCorresponds to variant dbSNP:rs1800454EnsemblClinVar.1
Natural variantiVAR_014998467V → I in allele TAP2*01F and allele TAP2*02D. 2 PublicationsCorresponds to variant dbSNP:rs150253319Ensembl.1
Natural variantiVAR_014999513A → S Rare polymorphism. 1
Natural variantiVAR_000095565A → T in allele TAP2*01:02, allele TAP2*01D, allele TAP2*02E and allele TAP2*02F. 1 PublicationCorresponds to variant dbSNP:rs2228396Ensembl.1
Natural variantiVAR_015000577M → V in allele TAP2*BKY2. 1 PublicationCorresponds to variant dbSNP:rs2228391Ensembl.1
Natural variantiVAR_000096651R → C in allele TAP2*01:03 and allele TAP2*01G. 1 PublicationCorresponds to variant dbSNP:rs4148876Ensembl.1
Natural variantiVAR_000097665T → A in allele TAP2*02:01, allele TAP2*02B, allele TAP2*02C, allele TAP2*02D, allele TAP2*02E, allele TAP2*02F, allele TAP2*04A and allele TAP2*Bky2. 2 PublicationsCorresponds to variant dbSNP:rs241447Ensembl.1
Natural variantiVAR_000098686L → LQEGQDLYSRLVQQRLMD in allele TAP2*02:01, allele TAP2*02B, allele TAP2*02C, allele TAP2*02D, allele TAP2*02E, allele TAP2*02F, allele TAP2*03A and allele TAP2*BKY2. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038904645 – 686LQDWN…KLAQL → KTLWKFMIF in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66401 Genomic DNA Translation: CAA47027.1
M84748 mRNA No translation available.
M74447 mRNA Translation: AAA59841.1
Z22935 mRNA Translation: CAA80522.1
Z22936 mRNA Translation: CAA80523.1
AB073779 mRNA Translation: BAB71769.1
AF105151 mRNA Translation: AAD12059.1
X87344 Genomic DNA Translation: CAA60788.1
AK222823 mRNA Translation: BAD96543.1
AK223300 mRNA Translation: BAD97020.1
BX296564 Genomic DNA No translation available.
CR788227 Genomic DNA No translation available.
BX682530 Genomic DNA No translation available.
CR762476 Genomic DNA No translation available.
CR753889 Genomic DNA No translation available.
CT009502 Genomic DNA No translation available.
BC002751 mRNA No translation available.
U07844 mRNA Translation: AAA79901.1
AH007554 Genomic DNA Translation: AAD23381.1
L09191 mRNA Translation: AAA58648.1
L10287 mRNA Translation: AAA58649.1
CCDSiCCDS4755.1 [Q03519-2]
CCDS78129.1 [Q03519-1]
PIRiB41538
RefSeqiNP_000535.3, NM_000544.3
NP_001276972.1, NM_001290043.1 [Q03519-1]
NP_061313.2, NM_018833.2 [Q03519-2]
UniGeneiHs.502

Genome annotation databases

EnsembliENST00000374897; ENSP00000364032; ENSG00000204267 [Q03519-1]
ENST00000374899; ENSP00000364034; ENSG00000204267 [Q03519-2]
ENST00000383118; ENSP00000372599; ENSG00000206235 [Q03519-1]
ENST00000383119; ENSP00000372600; ENSG00000206235 [Q03519-2]
ENST00000383239; ENSP00000372726; ENSG00000206299 [Q03519-1]
ENST00000383240; ENSP00000372727; ENSG00000206299 [Q03519-2]
ENST00000414145; ENSP00000401377; ENSG00000228582 [Q03519-2]
ENST00000419142; ENSP00000390013; ENSG00000237599
ENST00000426977; ENSP00000387553; ENSG00000232326 [Q03519-2]
ENST00000439425; ENSP00000396156; ENSG00000225967 [Q03519-2]
ENST00000443713; ENSP00000394101; ENSG00000228582 [Q03519-1]
ENST00000451907; ENSP00000392172; ENSG00000223481 [Q03519-2]
GeneIDi6891
KEGGihsa:6891
UCSCiuc063yci.1 human [Q03519-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTAP2_HUMAN
AccessioniPrimary (citable) accession number: Q03519
Secondary accession number(s): B0V2J8
, O95410, Q53FI6, Q5HY71, Q96PT8, Q9UQ83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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