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Protein

Thrombospondin-2

Gene

Thbs2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • negative regulation of angiogenesis Source: UniProtKB
  • positive regulation of synapse assembly Source: MGI

Keywordsi

Molecular functionHeparin-binding
Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-186797 Signaling by PDGF
R-MMU-5173214 O-glycosylation of TSR domain-containing proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-2
Gene namesi
Name:Thbs2
Synonyms:Tsp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98738 Thbs2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000003584719 – 1172Thrombospondin-2Add BLAST1154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi151N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi266InterchainCurated
Disulfide bondi270InterchainCurated
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi330N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi393 ↔ 425By similarity
Disulfide bondi397 ↔ 430By similarity
Disulfide bondi408 ↔ 415By similarity
Disulfide bondi449 ↔ 486By similarity
Disulfide bondi453 ↔ 491By similarity
Glycosylationi457N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi464 ↔ 476By similarity
Disulfide bondi506 ↔ 543By similarity
Disulfide bondi510 ↔ 548By similarity
Disulfide bondi521 ↔ 533By similarity
Disulfide bondi553 ↔ 564By similarity
Disulfide bondi558 ↔ 574By similarity
Disulfide bondi577 ↔ 588By similarity
Glycosylationi584N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi594 ↔ 610By similarity
Disulfide bondi601 ↔ 619By similarity
Disulfide bondi622 ↔ 646By similarity
Disulfide bondi652 ↔ 665By similarity
Disulfide bondi659 ↔ 678By similarity
Disulfide bondi680 ↔ 691By similarity
Disulfide bondi707 ↔ 715By similarity
Glycosylationi710N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi720 ↔ 740By similarity
Disulfide bondi756 ↔ 776By similarity
Disulfide bondi779 ↔ 799By similarity
Disulfide bondi815 ↔ 835By similarity
Disulfide bondi838 ↔ 858By similarity
Disulfide bondi876 ↔ 896By similarity
Disulfide bondi912 ↔ 932By similarity
Disulfide bondi948 ↔ 1169By similarity
Glycosylationi1069N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ03350
PaxDbiQ03350
PeptideAtlasiQ03350
PRIDEiQ03350

PTM databases

iPTMnetiQ03350
PhosphoSitePlusiQ03350

Expressioni

Gene expression databases

BgeeiENSMUSG00000023885
CleanExiMM_THBS2
GenevisibleiQ03350 MM

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Can bind to fibrinogen, fibronectin, laminin and type V collagen (By similarity). Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS2 with CD36. Can bind to fibrinogen, fibronectin, laminin.By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi204176, 2 interactors
ComplexPortaliCPX-3023 Thrombospondin 2 complex
IntActiQ03350, 3 interactors
MINTiQ03350
STRINGi10090.ENSMUSP00000128308

Structurei

3D structure databases

ProteinModelPortaliQ03350
SMRiQ03350
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 215Laminin G-likeAdd BLAST197
Domaini318 – 375VWFCPROSITE-ProRule annotationAdd BLAST58
Domaini381 – 431TSP type-1 1PROSITE-ProRule annotationAdd BLAST51
Domaini437 – 492TSP type-1 2PROSITE-ProRule annotationAdd BLAST56
Domaini494 – 549TSP type-1 3PROSITE-ProRule annotationAdd BLAST56
Domaini549 – 589EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini648 – 692EGF-like 2PROSITE-ProRule annotationAdd BLAST45
Repeati693 – 728TSP type-3 1Add BLAST36
Repeati729 – 764TSP type-3 2Add BLAST36
Repeati765 – 787TSP type-3 3Add BLAST23
Repeati788 – 823TSP type-3 4Add BLAST36
Repeati824 – 846TSP type-3 5Add BLAST23
Repeati847 – 884TSP type-3 6Add BLAST38
Repeati885 – 920TSP type-3 7Add BLAST36
Repeati921 – 956TSP type-3 8Add BLAST36
Domaini960 – 1172TSP C-terminalPROSITE-ProRule annotationAdd BLAST213

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 232Heparin-bindingSequence analysisAdd BLAST214

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi928 – 930Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the thrombospondin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ Eukaryota
ENOG410XQKE LUCA
GeneTreeiENSGT00850000132273
HOGENOMiHOG000007542
HOVERGENiHBG018006
InParanoidiQ03350
KOiK04659
OMAiTEKQVCE
OrthoDBiEOG091G00TV
TreeFamiTF324917

Family and domain databases

Gene3Di2.20.100.10, 3 hits
4.10.1080.10, 2 hits
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR024731 EGF_dom
IPR001791 Laminin_G
IPR037349 Thrombospondin
IPR003367 Thrombospondin_3-like_rpt
IPR017897 Thrombospondin_3_rpt
IPR008859 Thrombospondin_C
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
IPR015455 TSP2
IPR028974 TSP_type-3_rpt
IPR001007 VWF_dom
PANTHERiPTHR10199 PTHR10199, 2 hits
PTHR10199:SF10 PTHR10199:SF10, 2 hits
PfamiView protein in Pfam
PF12947 EGF_3, 1 hit
PF07645 EGF_CA, 1 hit
PF00090 TSP_1, 3 hits
PF02412 TSP_3, 7 hits
PF05735 TSP_C, 1 hit
PF00093 VWC, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00209 TSP1, 3 hits
SM00210 TSPN, 1 hit
SM00214 VWC, 1 hit
SUPFAMiSSF103647 SSF103647, 3 hits
SSF49899 SSF49899, 2 hits
SSF82895 SSF82895, 3 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS50092 TSP1, 3 hits
PS51234 TSP3, 8 hits
PS51236 TSP_CTER, 1 hit
PS01208 VWFC_1, 1 hit
PS50184 VWFC_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWALALLAL GIGPRASAGD HVKDTSFDLF SISNINRKTI GAKQFRGPDP
60 70 80 90 100
GVPAYRFVRF DYIPPVNTDD LNRIVKLARR KEGFFLTAQL KQDRKSRGTL
110 120 130 140 150
LVLEGPGTSQ RQFEIVSNGP GDTLDLNYWV EGNQHTNFLE DVGLADSQWK
160 170 180 190 200
NVTVQVASDT YSLYVGCDLI DSVTLEEPFY EQLEVDRSRM YVAKGASRES
210 220 230 240 250
HFRGLLQNVH LVFADSVEDI LSKKGCQHSQ GAEVNTISEH TETLHLSPHI
260 270 280 290 300
TTDLVVQGVE KAQEVCTHSC EELSNMMNEL SGLHVMVNQL SKNLERVSSD
310 320 330 340 350
NQFLLELIGG PLKTRNMSAC VQEGRIFAEN ETWVVDSCTT CTCKKFKTVC
360 370 380 390 400
HQITCSPATC ANPSFVEGEC CPSCSHSADS DEGWSPWAEW TECSVTCGSG
410 420 430 440 450
TQQRGRSCDV TSNTCLGPSI QTRTCSLGKC DTRIRQNGGW SHWSPWSSCS
460 470 480 490 500
VTCGVGNVTR IRLCNSPVPQ MGGKNCKGSG RETKPCQRDP CPIDGRWSPW
510 520 530 540 550
SPWSACTVTC AGGIRERSRV CNSPEPQYGG KDCVGDVTEH QMCNKRSCPI
560 570 580 590 600
DGCLSNPCFP GAKCNSFPDG SWSCGSCPVG FLGNGTHCED LDECAVVTDI
610 620 630 640 650
CFSTNKAPRC VNTNPGFHCL PCPPRYKGNQ PFGVGLEDAR TEKQVCEPEN
660 670 680 690 700
PCKDKTHSCH KNAECIYLGH FSDPMYKCEC QIGYAGDGLI CGEDSDLDGW
710 720 730 740 750
PNNNLVCATN ATYHCIKDNC PKLPNSGQED FDKDGIGDAC DEDDDNDGVS
760 770 780 790 800
DEKDNCQLLF NPRQLDYDKD EVGDRCDNCP YVHNPAQIDT DNNGEGDACS
810 820 830 840 850
VDIDGDDVFN ERDNCPYVYN TDQRDTDGDG VGDHCDNCPL MHNPDQIDQD
860 870 880 890 900
NDLVGDQCDN NEDIDDDGHQ NNQDNCPYIS NSNQADHDND GKGDACDSDD
910 920 930 940 950
DNDGVPDDRD NCRLVFNPDQ EDSDGDGRGD ICKDDFDNDN VPDIDDVCPE
960 970 980 990 1000
NNAITETDFR NFQMVPLDPK GTTQIDPNWV IRHQGKELVQ TANSDPGIAV
1010 1020 1030 1040 1050
GFDEFGSVDF SGTFYVNTDR DDDYAGFVFG YQSSSRFYVV MWKQVTQTYW
1060 1070 1080 1090 1100
EDKPSRAYGY SGVSLKVVNS TTGTGEHLRN ALWHTGNTEG QVRTLWHDPK
1110 1120 1130 1140 1150
NIGWKDYTAY RWHLIHRPKT GYMRVLVHEG KQVMADSGPI YDQTYAGGRL
1160 1170
GLFVFSQEMV YFSDLKYECR DA
Length:1,172
Mass (Da):129,882
Last modified:July 27, 2011 - v2
Checksum:i020ACD7EB5137B25
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225G → S in AAA53064 (PubMed:1371115).Curated1
Sequence conflicti225G → S in AAA40432 (PubMed:1712771).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07803 mRNA Translation: AAA53064.1
AF549256 Genomic DNA Translation: AAO16244.1
CH466630 Genomic DNA Translation: EDL20481.1
M64866 mRNA Translation: AAA40432.1
CCDSiCCDS49958.1
PIRiA42587
RefSeqiNP_035711.2, NM_011581.3
UniGeneiMm.26688

Genome annotation databases

EnsembliENSMUST00000170872; ENSMUSP00000128308; ENSMUSG00000023885
GeneIDi21826
KEGGimmu:21826
UCSCiuc008anb.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTSP2_MOUSE
AccessioniPrimary (citable) accession number: Q03350
Secondary accession number(s): Q8CG21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: July 18, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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